Chicken stool associated circular virus 2

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346BLF0|A0A346BLF0_9VIRU Capsid protein OS=Chicken stool associated circular virus 2 OX=2109374 PE=4 SV=1
MM1 pKa = 7.94RR2 pKa = 11.84SNFVISNSVANFRR15 pKa = 11.84ARR17 pKa = 11.84RR18 pKa = 11.84DD19 pKa = 3.05IEE21 pKa = 4.04TAAKK25 pKa = 9.75RR26 pKa = 11.84WCFTINNPTDD36 pKa = 3.57RR37 pKa = 11.84DD38 pKa = 4.12RR39 pKa = 11.84FWEE42 pKa = 4.38SPDD45 pKa = 3.59ALEE48 pKa = 3.8QLSYY52 pKa = 10.77IIVQEE57 pKa = 3.92EE58 pKa = 3.92RR59 pKa = 11.84GEE61 pKa = 4.23NGTVHH66 pKa = 5.51YY67 pKa = 10.46QGFLILKK74 pKa = 9.08KK75 pKa = 10.1RR76 pKa = 11.84NRR78 pKa = 11.84VTWLKK83 pKa = 11.11RR84 pKa = 11.84NMNEE88 pKa = 3.56RR89 pKa = 11.84AHH91 pKa = 5.82WEE93 pKa = 4.07KK94 pKa = 10.25TRR96 pKa = 11.84GTDD99 pKa = 3.39KK100 pKa = 10.91QAADD104 pKa = 3.9YY105 pKa = 10.55CRR107 pKa = 11.84KK108 pKa = 10.24DD109 pKa = 3.56DD110 pKa = 3.53THH112 pKa = 8.26VPGGLRR118 pKa = 11.84FEE120 pKa = 4.79HH121 pKa = 6.37GKK123 pKa = 9.44PPRR126 pKa = 11.84EE127 pKa = 4.14KK128 pKa = 10.17KK129 pKa = 10.55DD130 pKa = 3.35KK131 pKa = 9.81EE132 pKa = 4.04QEE134 pKa = 3.93ANEE137 pKa = 4.13ILDD140 pKa = 3.9SLRR143 pKa = 11.84TGYY146 pKa = 10.13KK147 pKa = 9.75RR148 pKa = 11.84PQEE151 pKa = 4.08IPAEE155 pKa = 4.11LLRR158 pKa = 11.84SRR160 pKa = 11.84NFISAYY166 pKa = 9.9KK167 pKa = 10.67LITTDD172 pKa = 2.79VLGPFRR178 pKa = 11.84PNLKK182 pKa = 9.92IITLVGPPGCGKK194 pKa = 10.54SYY196 pKa = 10.46LINYY200 pKa = 7.58LFPDD204 pKa = 4.32CARR207 pKa = 11.84LIMGNAGFWWQNPCSKK223 pKa = 10.09VACIEE228 pKa = 4.04EE229 pKa = 4.22FAGQIPLQKK238 pKa = 9.64MLNLLDD244 pKa = 5.27PYY246 pKa = 10.09PQSLEE251 pKa = 4.25VKK253 pKa = 10.22GGTRR257 pKa = 11.84PCLFEE262 pKa = 5.05VIFITSNSCPEE273 pKa = 3.2DD274 pKa = 3.02WYY276 pKa = 10.94RR277 pKa = 11.84NKK279 pKa = 10.07VPGIPEE285 pKa = 3.48EE286 pKa = 4.26AKK288 pKa = 10.72RR289 pKa = 11.84HH290 pKa = 5.5DD291 pKa = 4.61ALLALYY297 pKa = 10.01DD298 pKa = 3.62RR299 pKa = 11.84LGYY302 pKa = 10.07HH303 pKa = 6.7PAWHH307 pKa = 6.72HH308 pKa = 5.76GALEE312 pKa = 4.32RR313 pKa = 11.84TCGHH317 pKa = 5.91YY318 pKa = 10.71LEE320 pKa = 5.5PPQLGAITPEE330 pKa = 4.59WIQEE334 pKa = 3.92TRR336 pKa = 11.84KK337 pKa = 8.9WLLRR341 pKa = 11.84EE342 pKa = 3.71VRR344 pKa = 11.84RR345 pKa = 11.84ILEE348 pKa = 4.11MPEE351 pKa = 3.88EE352 pKa = 4.21QPAAAAAAAQPEE364 pKa = 4.3EE365 pKa = 4.54QPTQRR370 pKa = 11.84LEE372 pKa = 3.96EE373 pKa = 4.42AEE375 pKa = 4.64EE376 pKa = 4.36FPEE379 pKa = 4.17HH380 pKa = 6.37QPEE383 pKa = 4.3LTLLEE388 pKa = 4.56TTTSSQTSITLKK400 pKa = 10.59LSADD404 pKa = 3.43KK405 pKa = 10.06THH407 pKa = 6.97LGKK410 pKa = 9.23TSTSSKK416 pKa = 10.28HH417 pKa = 5.72KK418 pKa = 10.6AHH420 pKa = 6.27NTQDD424 pKa = 3.01THH426 pKa = 4.28STKK429 pKa = 10.61RR430 pKa = 11.84KK431 pKa = 8.89FVSQLRR437 pKa = 11.84QLKK440 pKa = 9.58LWAVV444 pKa = 3.6

Molecular weight: 51.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346BLF0|A0A346BLF0_9VIRU Capsid protein OS=Chicken stool associated circular virus 2 OX=2109374 PE=4 SV=1
MM1 pKa = 7.6PRR3 pKa = 11.84YY4 pKa = 9.49KK5 pKa = 10.32SRR7 pKa = 11.84RR8 pKa = 11.84TSSRR12 pKa = 11.84YY13 pKa = 6.91SRR15 pKa = 11.84RR16 pKa = 11.84YY17 pKa = 8.52RR18 pKa = 11.84RR19 pKa = 11.84YY20 pKa = 8.67RR21 pKa = 11.84RR22 pKa = 11.84YY23 pKa = 9.88RR24 pKa = 11.84RR25 pKa = 11.84YY26 pKa = 9.85ARR28 pKa = 11.84IPRR31 pKa = 11.84RR32 pKa = 11.84VSYY35 pKa = 11.52ANGRR39 pKa = 11.84DD40 pKa = 3.48SSRR43 pKa = 11.84TNIKK47 pKa = 10.23VVTTTYY53 pKa = 10.57RR54 pKa = 11.84AGDD57 pKa = 3.46IAAGANDD64 pKa = 4.55SIVFTLPAFSRR75 pKa = 11.84TGGSGPSIVDD85 pKa = 3.77SICVLNSPLYY95 pKa = 9.59RR96 pKa = 11.84AYY98 pKa = 9.26TSLYY102 pKa = 10.63EE103 pKa = 4.07EE104 pKa = 4.17VLLRR108 pKa = 11.84GVSYY112 pKa = 10.55EE113 pKa = 3.75ITVNNPIGASATTLPPFMIFTSVDD137 pKa = 2.46RR138 pKa = 11.84RR139 pKa = 11.84YY140 pKa = 10.72AYY142 pKa = 10.61GEE144 pKa = 4.13VPPSQSQLTNYY155 pKa = 10.07SSSKK159 pKa = 8.56PISYY163 pKa = 8.03VTYY166 pKa = 10.41NVTKK170 pKa = 9.18FRR172 pKa = 11.84KK173 pKa = 9.16YY174 pKa = 9.91VGARR178 pKa = 11.84DD179 pKa = 3.82LVEE182 pKa = 3.63KK183 pKa = 8.91TQYY186 pKa = 10.64HH187 pKa = 7.25DD188 pKa = 3.83CTVSYY193 pKa = 10.56YY194 pKa = 11.1NSGGNQYY201 pKa = 10.16WYY203 pKa = 10.51DD204 pKa = 3.59PAVNSAGVNPNFFNPALFFFVRR226 pKa = 11.84FPFAPTSTMQLQLSIRR242 pKa = 11.84CTFYY246 pKa = 10.53LTFRR250 pKa = 11.84NPAPFDD256 pKa = 3.94SVPVPAAAAAAASGPFEE273 pKa = 4.28SFSTLSADD281 pKa = 3.9EE282 pKa = 4.12QLLDD286 pKa = 4.46IPDD289 pKa = 3.97PHH291 pKa = 7.0PLSGSTDD298 pKa = 3.42PPLIEE303 pKa = 4.13TT304 pKa = 4.52

Molecular weight: 34.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

748

304

444

374.0

42.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.62 ± 0.36

1.604 ± 0.368

4.144 ± 0.079

6.283 ± 2.177

4.278 ± 0.783

5.08 ± 0.087

2.139 ± 0.883

4.813 ± 0.32

4.679 ± 1.613

8.155 ± 1.332

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.07 ± 0.049

4.545 ± 0.232

7.487 ± 0.635

3.743 ± 0.859

8.289 ± 0.549

7.62 ± 2.32

7.219 ± 0.403

4.813 ± 1.249

1.604 ± 0.76

4.813 ± 1.837
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski