Rottboellia yellow mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Sobemovirus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3ISH8|A0A0E3ISH8_9VIRU Putative Px OS=Rottboellia yellow mottle virus OX=1432563 GN=ORFx PE=4 SV=1
MM1 pKa = 7.11TRR3 pKa = 11.84LEE5 pKa = 4.37IKK7 pKa = 9.54VHH9 pKa = 6.3PSQTTASAFRR19 pKa = 11.84AYY21 pKa = 10.3YY22 pKa = 10.66GSGPLYY28 pKa = 9.35KK29 pKa = 9.69WAVHH33 pKa = 5.44SCEE36 pKa = 4.05IDD38 pKa = 3.52DD39 pKa = 4.56VEE41 pKa = 5.33IEE43 pKa = 3.86GDD45 pKa = 3.57YY46 pKa = 11.06DD47 pKa = 4.12YY48 pKa = 11.55DD49 pKa = 3.77SCSYY53 pKa = 10.87LYY55 pKa = 11.0VEE57 pKa = 5.52ISCEE61 pKa = 4.26SCGKK65 pKa = 8.0TLEE68 pKa = 4.52DD69 pKa = 4.2FSVEE73 pKa = 3.55VDD75 pKa = 3.39RR76 pKa = 11.84FRR78 pKa = 11.84SLSPSRR84 pKa = 11.84IEE86 pKa = 4.14ATCEE90 pKa = 3.75QYY92 pKa = 10.92NCKK95 pKa = 10.26CGEE98 pKa = 4.15EE99 pKa = 4.21EE100 pKa = 4.33SSEE103 pKa = 4.19DD104 pKa = 3.67EE105 pKa = 3.91EE106 pKa = 5.47AEE108 pKa = 4.33FYY110 pKa = 11.17SEE112 pKa = 4.35FLSKK116 pKa = 10.32FDD118 pKa = 3.88RR119 pKa = 11.84LSAGEE124 pKa = 3.89VSGAFTAQVDD134 pKa = 3.84SDD136 pKa = 4.17SEE138 pKa = 4.32EE139 pKa = 4.16EE140 pKa = 3.73IVYY143 pKa = 10.11QGRR146 pKa = 11.84GGHH149 pKa = 7.07DD150 pKa = 3.11SDD152 pKa = 4.52

Molecular weight: 17.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3D8Q7|A0A0E3D8Q7_9VIRU Putative RNA silencing suppressor OS=Rottboellia yellow mottle virus OX=1432563 GN=P1 PE=4 SV=1
MM1 pKa = 7.14TKK3 pKa = 10.02RR4 pKa = 11.84RR5 pKa = 11.84TRR7 pKa = 11.84ARR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 8.39ATKK14 pKa = 9.44TSNRR18 pKa = 11.84TPNEE22 pKa = 3.64RR23 pKa = 11.84RR24 pKa = 11.84TMEE27 pKa = 4.01SGTRR31 pKa = 11.84SVSRR35 pKa = 11.84VSAPATAGALISYY48 pKa = 9.74RR49 pKa = 11.84GGISWSPAAGTTIVTNSGSLAVVQSSATAGAEE81 pKa = 3.94VTGTVNFNVRR91 pKa = 11.84NATEE95 pKa = 4.58LPWLSTFGPAFSKK108 pKa = 10.97YY109 pKa = 8.2RR110 pKa = 11.84VKK112 pKa = 10.65SASFTWEE119 pKa = 3.94PVVGTSTNGEE129 pKa = 3.52ICMALLYY136 pKa = 10.72DD137 pKa = 3.92QADD140 pKa = 3.82PAASSLSIRR149 pKa = 11.84RR150 pKa = 11.84LMQTSRR156 pKa = 11.84SEE158 pKa = 3.86WGPIWARR165 pKa = 11.84SKK167 pKa = 10.88QPVVVDD173 pKa = 3.59ISRR176 pKa = 11.84MSLKK180 pKa = 9.88WYY182 pKa = 10.44LSGATAGVAAGNFQTPFALAYY203 pKa = 9.33AAQSSTTSTNLGRR216 pKa = 11.84IMVQYY221 pKa = 9.58VVEE224 pKa = 5.37FIEE227 pKa = 4.75PVDD230 pKa = 3.59PTVNSS235 pKa = 3.68

Molecular weight: 25.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1981

106

934

396.2

43.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.228 ± 0.982

1.868 ± 0.408

4.846 ± 0.652

8.43 ± 0.967

3.079 ± 0.379

7.521 ± 0.381

1.767 ± 0.31

3.584 ± 0.297

4.745 ± 0.632

8.127 ± 1.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.868 ± 0.217

2.272 ± 0.344

4.796 ± 0.448

3.332 ± 0.351

7.118 ± 0.914

11.005 ± 0.866

4.695 ± 1.284

7.32 ± 0.405

2.322 ± 0.312

3.079 ± 0.546
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski