Pectobacterium phage Wc4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ounavirinae; unclassified Ounavirinae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 145 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8D407|A0A5P8D407_9CAUD Uncharacterized protein OS=Pectobacterium phage Wc4 OX=2652428 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.03CFHH5 pKa = 6.21GTSLAKK11 pKa = 10.35AIEE14 pKa = 4.59IINGSTEE21 pKa = 3.97KK22 pKa = 10.45DD23 pKa = 2.9TGLWNCCDD31 pKa = 3.81RR32 pKa = 11.84DD33 pKa = 3.73GAFYY37 pKa = 10.62VYY39 pKa = 9.32PQNKK43 pKa = 9.03IISSQCLDD51 pKa = 3.7EE52 pKa = 6.55DD53 pKa = 4.25EE54 pKa = 5.71EE55 pKa = 4.42DD56 pKa = 3.68LQEE59 pKa = 4.92QVLSWGVSNSLWHH72 pKa = 6.3AQSATCKK79 pKa = 10.02EE80 pKa = 3.93LAKK83 pKa = 10.94DD84 pKa = 3.32EE85 pKa = 4.66TYY87 pKa = 11.03VILEE91 pKa = 3.77MDD93 pKa = 3.9IPDD96 pKa = 4.55EE97 pKa = 4.36LLQDD101 pKa = 3.51DD102 pKa = 4.53HH103 pKa = 7.1SANNMEE109 pKa = 4.41SQASFITEE117 pKa = 4.3DD118 pKa = 3.76DD119 pKa = 3.75MEE121 pKa = 6.15SITFQVCHH129 pKa = 5.86VGQLNVYY136 pKa = 9.68LRR138 pKa = 11.84PFVLASVIDD147 pKa = 3.81NVNFGDD153 pKa = 3.87FGVDD157 pKa = 3.24EE158 pKa = 5.13SLLSMAQLLAANNVWLNTEE177 pKa = 3.66EE178 pKa = 4.23DD179 pKa = 3.71ANLSVFPINDD189 pKa = 3.2FKK191 pKa = 11.2EE192 pKa = 4.07IYY194 pKa = 10.29INAA197 pKa = 4.2

Molecular weight: 22.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8D5A3|A0A5P8D5A3_9CAUD Uncharacterized protein OS=Pectobacterium phage Wc4 OX=2652428 PE=4 SV=1
MM1 pKa = 6.87FTKK4 pKa = 10.52KK5 pKa = 10.47NLIIAAVVTGLFILMVSSCGSDD27 pKa = 2.75KK28 pKa = 11.19TEE30 pKa = 3.58QTAQYY35 pKa = 8.35VQPVQQAPAQQVMQQAPVQEE55 pKa = 4.22RR56 pKa = 11.84QPVYY60 pKa = 10.62DD61 pKa = 3.5QNQPNVVVVQQPPQTVVQDD80 pKa = 3.6SGGITAGHH88 pKa = 7.25LAAGALGYY96 pKa = 9.99FAGRR100 pKa = 11.84SSSSNSNRR108 pKa = 11.84GYY110 pKa = 8.87SQRR113 pKa = 11.84TVTNNRR119 pKa = 11.84TTIIKK124 pKa = 8.96QAPRR128 pKa = 11.84QKK130 pKa = 10.82SYY132 pKa = 10.73FGSSSRR138 pKa = 11.84TRR140 pKa = 11.84TKK142 pKa = 10.54SFTSSRR148 pKa = 11.84RR149 pKa = 11.84RR150 pKa = 3.35

Molecular weight: 16.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

145

0

145

26727

35

1041

184.3

20.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.94 ± 0.321

1.321 ± 0.107

5.942 ± 0.17

6.177 ± 0.243

4.131 ± 0.13

7.023 ± 0.224

2.005 ± 0.113

5.811 ± 0.166

6.357 ± 0.291

8.093 ± 0.153

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.84 ± 0.128

4.886 ± 0.16

3.274 ± 0.124

3.985 ± 0.127

4.939 ± 0.169

6.69 ± 0.271

6.364 ± 0.271

7.236 ± 0.199

1.414 ± 0.079

3.573 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski