Streptococcus phage Javan150

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AS95|A0A4D6AS95_9CAUD Endopeptidase OS=Streptococcus phage Javan150 OX=2548008 GN=Javan150_0043 PE=4 SV=1
MM1 pKa = 7.83AIYY4 pKa = 10.41INTKK8 pKa = 6.94TRR10 pKa = 11.84NTIEE14 pKa = 3.88TDD16 pKa = 3.7LVVSGGDD23 pKa = 3.14WEE25 pKa = 5.13LVGEE29 pKa = 4.26QQASDD34 pKa = 3.91KK35 pKa = 10.74EE36 pKa = 4.36PTVPEE41 pKa = 4.5LKK43 pKa = 10.67AKK45 pKa = 10.43LDD47 pKa = 3.87EE48 pKa = 5.54LGIDD52 pKa = 3.65YY53 pKa = 10.3DD54 pKa = 4.17KK55 pKa = 11.08KK56 pKa = 10.65AKK58 pKa = 10.29KK59 pKa = 9.95PEE61 pKa = 4.03LLALLEE67 pKa = 4.45SAEE70 pKa = 4.39SNQDD74 pKa = 3.01EE75 pKa = 4.66AEE77 pKa = 4.01

Molecular weight: 8.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AUN8|A0A4D6AUN8_9CAUD Hyaluronidase OS=Streptococcus phage Javan150 OX=2548008 GN=Javan150_0046 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.66FKK5 pKa = 10.93FKK7 pKa = 11.01LNRR10 pKa = 11.84AGVAEE15 pKa = 4.22LMKK18 pKa = 10.49SSEE21 pKa = 4.17MQQVLTTKK29 pKa = 9.48ATAIRR34 pKa = 11.84EE35 pKa = 4.25RR36 pKa = 11.84CGDD39 pKa = 3.82GYY41 pKa = 9.01TQDD44 pKa = 2.62IHH46 pKa = 7.83VGKK49 pKa = 10.1NRR51 pKa = 11.84ANAMVSAKK59 pKa = 9.54TIKK62 pKa = 10.38AKK64 pKa = 10.43KK65 pKa = 10.15DD66 pKa = 3.23NSKK69 pKa = 11.36NNTLLKK75 pKa = 10.41AVRR78 pKa = 3.79

Molecular weight: 8.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

10342

45

785

206.8

23.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.078 ± 0.647

0.629 ± 0.127

6.73 ± 0.335

7.078 ± 0.472

4.08 ± 0.24

6.981 ± 0.588

1.47 ± 0.185

6.981 ± 0.34

8.896 ± 0.455

8.093 ± 0.249

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.495 ± 0.198

5.096 ± 0.218

3.007 ± 0.274

3.897 ± 0.22

3.79 ± 0.333

6.246 ± 0.425

6.179 ± 0.296

6.604 ± 0.252

1.131 ± 0.122

3.539 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski