Pseudomonas phage Fc02

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5A4N4R4|A0A5A4N4R4_9CAUD Uncharacterized protein OS=Pseudomonas phage Fc02 OX=2301634 GN=Fc02_25 PE=4 SV=1
MM1 pKa = 7.5SEE3 pKa = 4.53SINLQALLQRR13 pKa = 11.84LDD15 pKa = 3.53EE16 pKa = 4.09QAYY19 pKa = 8.97EE20 pKa = 4.0QLCIEE25 pKa = 4.66AARR28 pKa = 11.84LAEE31 pKa = 4.12EE32 pKa = 4.07NEE34 pKa = 4.05YY35 pKa = 11.08LRR37 pKa = 11.84TEE39 pKa = 4.43LTRR42 pKa = 11.84MEE44 pKa = 4.14EE45 pKa = 4.64CAEE48 pKa = 4.3GWCNEE53 pKa = 3.86AQHH56 pKa = 6.49LHH58 pKa = 5.01QQLAEE63 pKa = 4.15ATGGQAAITQSGALVVIPMEE83 pKa = 4.45RR84 pKa = 11.84CAA86 pKa = 5.14

Molecular weight: 9.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5A4N1E9|A0A5A4N1E9_9CAUD Uncharacterized protein OS=Pseudomonas phage Fc02 OX=2301634 GN=Fc02_20 PE=4 SV=1
MM1 pKa = 7.2NVVPITGRR9 pKa = 11.84LPEE12 pKa = 4.3EE13 pKa = 3.95QPKK16 pKa = 10.5AIHH19 pKa = 6.72LPLCTVLTPEE29 pKa = 5.47LARR32 pKa = 11.84CLEE35 pKa = 4.42AVNSATRR42 pKa = 11.84ALRR45 pKa = 11.84QAGIPIDD52 pKa = 3.76QTSLLDD58 pKa = 3.21RR59 pKa = 11.84RR60 pKa = 11.84LFIRR64 pKa = 11.84EE65 pKa = 3.75EE66 pKa = 4.02DD67 pKa = 3.45SLRR70 pKa = 11.84LHH72 pKa = 6.35RR73 pKa = 11.84RR74 pKa = 11.84FRR76 pKa = 11.84NAIRR80 pKa = 11.84GIRR83 pKa = 11.84QTTRR87 pKa = 11.84GMVTVHH93 pKa = 5.57VVSLLGVDD101 pKa = 4.34VAWTTPVKK109 pKa = 10.67EE110 pKa = 4.08QDD112 pKa = 3.17QQ113 pKa = 3.69

Molecular weight: 12.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

12330

47

1210

228.3

25.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.352 ± 0.718

0.835 ± 0.126

5.807 ± 0.19

6.472 ± 0.32

2.887 ± 0.186

7.632 ± 0.359

1.768 ± 0.188

4.185 ± 0.224

3.796 ± 0.329

10.211 ± 0.312

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.133 ± 0.108

3.049 ± 0.195

4.818 ± 0.348

5.182 ± 0.328

7.388 ± 0.319

5.726 ± 0.26

5.288 ± 0.24

6.358 ± 0.253

1.76 ± 0.158

2.352 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski