Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Syntrophobacterales; Syntrophobacteraceae; Syntrophobacter; Syntrophobacter fumaroxidans

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4012 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0LNC8|A0LNC8_SYNFM GGDEF domain containing protein OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) OX=335543 GN=Sfum_3257 PE=4 SV=1
MM1 pKa = 7.79DD2 pKa = 5.68RR3 pKa = 11.84YY4 pKa = 10.51VCTVCGYY11 pKa = 10.86VYY13 pKa = 10.59DD14 pKa = 4.41PAVGDD19 pKa = 4.29PDD21 pKa = 4.78NDD23 pKa = 3.36IAPGTKK29 pKa = 9.91FEE31 pKa = 5.42DD32 pKa = 4.18LPEE35 pKa = 4.74DD36 pKa = 3.76WVCPVCGAAKK46 pKa = 10.34SDD48 pKa = 3.91FEE50 pKa = 4.73KK51 pKa = 10.88EE52 pKa = 3.86

Molecular weight: 5.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0LIX5|A0LIX5_SYNFM Alpha/beta hydrolase family protein OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) OX=335543 GN=Sfum_1690 PE=4 SV=1
MM1 pKa = 7.44LCGRR5 pKa = 11.84GGNAALSGVVSYY17 pKa = 11.27DD18 pKa = 3.39VVEE21 pKa = 4.6CMCRR25 pKa = 11.84PPRR28 pKa = 11.84EE29 pKa = 4.17PAGEE33 pKa = 3.77RR34 pKa = 11.84PAANGAMAAAGVEE47 pKa = 3.89MRR49 pKa = 11.84GRR51 pKa = 11.84GSRR54 pKa = 11.84APAVLSRR61 pKa = 11.84RR62 pKa = 11.84KK63 pKa = 7.82TVGKK67 pKa = 10.16APEE70 pKa = 4.23RR71 pKa = 11.84MTSAGSEE78 pKa = 3.94RR79 pKa = 11.84KK80 pKa = 9.46

Molecular weight: 8.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4012

0

4012

1348052

38

2031

336.0

37.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.327 ± 0.042

1.378 ± 0.017

5.192 ± 0.024

6.618 ± 0.041

4.139 ± 0.022

8.27 ± 0.036

2.133 ± 0.018

5.505 ± 0.031

4.277 ± 0.037

10.173 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.492 ± 0.018

2.885 ± 0.021

5.071 ± 0.026

2.932 ± 0.027

7.499 ± 0.037

5.728 ± 0.029

4.866 ± 0.026

7.606 ± 0.033

1.21 ± 0.015

2.698 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski