Amino acid dipepetide frequency for Synechococcus phage S-CAM9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.891AlaAla: 4.891 ± 0.382
0.552AlaCys: 0.552 ± 0.097
3.218AlaAsp: 3.218 ± 0.261
3.585AlaGlu: 3.585 ± 0.261
2.372AlaPhe: 2.372 ± 0.236
5.24AlaGly: 5.24 ± 0.503
0.974AlaHis: 0.974 ± 0.139
3.769AlaIle: 3.769 ± 0.26
3.475AlaLys: 3.475 ± 0.341
4.321AlaLeu: 4.321 ± 0.449
1.25AlaMet: 1.25 ± 0.17
3.438AlaAsn: 3.438 ± 0.267
2.28AlaPro: 2.28 ± 0.229
2.151AlaGln: 2.151 ± 0.211
2.354AlaArg: 2.354 ± 0.194
4.082AlaSer: 4.082 ± 0.271
5.277AlaThr: 5.277 ± 0.863
3.824AlaVal: 3.824 ± 0.265
0.662AlaTrp: 0.662 ± 0.142
2.17AlaTyr: 2.17 ± 0.194
0.0AlaXaa: 0.0 ± 0.0
Cys
0.588CysAla: 0.588 ± 0.1
0.092CysCys: 0.092 ± 0.04
0.754CysAsp: 0.754 ± 0.134
0.699CysGlu: 0.699 ± 0.133
0.423CysPhe: 0.423 ± 0.09
0.662CysGly: 0.662 ± 0.149
0.239CysHis: 0.239 ± 0.06
0.552CysIle: 0.552 ± 0.1
0.552CysLys: 0.552 ± 0.127
0.607CysLeu: 0.607 ± 0.123
0.221CysMet: 0.221 ± 0.075
0.478CysAsn: 0.478 ± 0.094
0.405CysPro: 0.405 ± 0.086
0.294CysGln: 0.294 ± 0.085
0.496CysArg: 0.496 ± 0.091
0.478CysSer: 0.478 ± 0.096
0.552CysThr: 0.552 ± 0.102
0.662CysVal: 0.662 ± 0.119
0.147CysTrp: 0.147 ± 0.054
0.515CysTyr: 0.515 ± 0.102
0.0CysXaa: 0.0 ± 0.0
Asp
4.174AspAla: 4.174 ± 0.363
0.625AspCys: 0.625 ± 0.121
4.302AspAsp: 4.302 ± 0.325
4.266AspGlu: 4.266 ± 0.357
3.181AspPhe: 3.181 ± 0.239
5.056AspGly: 5.056 ± 0.337
1.14AspHis: 1.14 ± 0.178
4.192AspIle: 4.192 ± 0.332
3.383AspLys: 3.383 ± 0.268
5.553AspLeu: 5.553 ± 0.298
1.361AspMet: 1.361 ± 0.232
3.493AspAsn: 3.493 ± 0.328
3.089AspPro: 3.089 ± 0.26
1.839AspGln: 1.839 ± 0.206
2.666AspArg: 2.666 ± 0.199
4.211AspSer: 4.211 ± 0.273
3.916AspThr: 3.916 ± 0.257
4.633AspVal: 4.633 ± 0.247
1.14AspTrp: 1.14 ± 0.163
3.549AspTyr: 3.549 ± 0.259
0.0AspXaa: 0.0 ± 0.0
Glu
3.475GluAla: 3.475 ± 0.306
0.699GluCys: 0.699 ± 0.131
4.523GluAsp: 4.523 ± 0.367
4.781GluGlu: 4.781 ± 0.65
3.273GluPhe: 3.273 ± 0.231
3.806GluGly: 3.806 ± 0.345
1.103GluHis: 1.103 ± 0.163
4.872GluIle: 4.872 ± 0.308
4.394GluLys: 4.394 ± 0.435
4.946GluLeu: 4.946 ± 0.403
1.71GluMet: 1.71 ± 0.255
3.402GluAsn: 3.402 ± 0.259
2.17GluPro: 2.17 ± 0.228
2.354GluGln: 2.354 ± 0.253
2.905GluArg: 2.905 ± 0.262
3.806GluSer: 3.806 ± 0.316
3.641GluThr: 3.641 ± 0.279
4.744GluVal: 4.744 ± 0.327
0.827GluTrp: 0.827 ± 0.159
3.071GluTyr: 3.071 ± 0.289
0.0GluXaa: 0.0 ± 0.0
Phe
2.611PheAla: 2.611 ± 0.241
0.607PheCys: 0.607 ± 0.123
3.42PheAsp: 3.42 ± 0.24
3.052PheGlu: 3.052 ± 0.261
1.839PhePhe: 1.839 ± 0.224
3.181PheGly: 3.181 ± 0.255
0.791PheHis: 0.791 ± 0.157
2.979PheIle: 2.979 ± 0.225
2.758PheLys: 2.758 ± 0.223
3.107PheLeu: 3.107 ± 0.291
1.232PheMet: 1.232 ± 0.157
2.96PheAsn: 2.96 ± 0.266
1.802PhePro: 1.802 ± 0.191
1.967PheGln: 1.967 ± 0.181
1.655PheArg: 1.655 ± 0.186
3.089PheSer: 3.089 ± 0.281
3.199PheThr: 3.199 ± 0.284
3.328PheVal: 3.328 ± 0.275
0.515PheTrp: 0.515 ± 0.097
1.508PheTyr: 1.508 ± 0.161
0.0PheXaa: 0.0 ± 0.0
Gly
4.523GlyAla: 4.523 ± 0.458
0.699GlyCys: 0.699 ± 0.113
4.468GlyAsp: 4.468 ± 0.374
4.247GlyGlu: 4.247 ± 0.323
3.714GlyPhe: 3.714 ± 0.265
5.479GlyGly: 5.479 ± 0.437
1.195GlyHis: 1.195 ± 0.17
5.737GlyIle: 5.737 ± 0.628
3.88GlyLys: 3.88 ± 0.349
5.056GlyLeu: 5.056 ± 0.294
1.471GlyMet: 1.471 ± 0.171
3.641GlyAsn: 3.641 ± 0.363
2.574GlyPro: 2.574 ± 0.587
3.291GlyGln: 3.291 ± 0.36
3.034GlyArg: 3.034 ± 0.292
5.682GlySer: 5.682 ± 0.469
6.748GlyThr: 6.748 ± 0.725
5.829GlyVal: 5.829 ± 0.523
0.938GlyTrp: 0.938 ± 0.147
3.107GlyTyr: 3.107 ± 0.351
0.0GlyXaa: 0.0 ± 0.0
His
0.846HisAla: 0.846 ± 0.148
0.257HisCys: 0.257 ± 0.076
0.938HisAsp: 0.938 ± 0.158
1.526HisGlu: 1.526 ± 0.213
0.827HisPhe: 0.827 ± 0.155
1.434HisGly: 1.434 ± 0.174
0.496HisHis: 0.496 ± 0.123
0.791HisIle: 0.791 ± 0.119
1.011HisLys: 1.011 ± 0.143
1.158HisLeu: 1.158 ± 0.183
0.331HisMet: 0.331 ± 0.082
0.827HisAsn: 0.827 ± 0.12
0.883HisPro: 0.883 ± 0.134
0.46HisGln: 0.46 ± 0.096
0.699HisArg: 0.699 ± 0.114
0.864HisSer: 0.864 ± 0.143
1.158HisThr: 1.158 ± 0.173
1.14HisVal: 1.14 ± 0.147
0.368HisTrp: 0.368 ± 0.088
0.919HisTyr: 0.919 ± 0.151
0.0HisXaa: 0.0 ± 0.0
Ile
4.284IleAla: 4.284 ± 0.316
0.552IleCys: 0.552 ± 0.105
4.707IleAsp: 4.707 ± 0.286
4.468IleGlu: 4.468 ± 0.275
2.556IlePhe: 2.556 ± 0.248
4.909IleGly: 4.909 ± 0.549
1.048IleHis: 1.048 ± 0.142
3.861IleIle: 3.861 ± 0.314
4.284IleLys: 4.284 ± 0.299
4.615IleLeu: 4.615 ± 0.27
1.177IleMet: 1.177 ± 0.185
4.321IleAsn: 4.321 ± 0.451
2.335IlePro: 2.335 ± 0.239
2.574IleGln: 2.574 ± 0.223
3.034IleArg: 3.034 ± 0.233
4.56IleSer: 4.56 ± 0.353
5.112IleThr: 5.112 ± 0.452
4.137IleVal: 4.137 ± 0.395
0.588IleTrp: 0.588 ± 0.136
2.188IleTyr: 2.188 ± 0.222
0.0IleXaa: 0.0 ± 0.0
Lys
2.868LysAla: 2.868 ± 0.255
0.533LysCys: 0.533 ± 0.131
3.788LysAsp: 3.788 ± 0.331
4.505LysGlu: 4.505 ± 0.471
2.482LysPhe: 2.482 ± 0.248
3.585LysGly: 3.585 ± 0.438
0.791LysHis: 0.791 ± 0.123
4.266LysIle: 4.266 ± 0.397
5.387LysLys: 5.387 ± 0.761
5.24LysLeu: 5.24 ± 0.346
1.397LysMet: 1.397 ± 0.197
3.383LysAsn: 3.383 ± 0.234
2.464LysPro: 2.464 ± 0.271
2.225LysGln: 2.225 ± 0.228
2.501LysArg: 2.501 ± 0.272
4.063LysSer: 4.063 ± 0.33
3.714LysThr: 3.714 ± 0.299
4.247LysVal: 4.247 ± 0.308
0.754LysTrp: 0.754 ± 0.172
3.365LysTyr: 3.365 ± 0.297
0.0LysXaa: 0.0 ± 0.0
Leu
4.321LeuAla: 4.321 ± 0.334
0.607LeuCys: 0.607 ± 0.106
5.02LeuAsp: 5.02 ± 0.377
4.909LeuGlu: 4.909 ± 0.389
2.923LeuPhe: 2.923 ± 0.244
5.167LeuGly: 5.167 ± 0.326
1.25LeuHis: 1.25 ± 0.191
4.155LeuIle: 4.155 ± 0.287
5.13LeuLys: 5.13 ± 0.36
4.266LeuLeu: 4.266 ± 0.474
1.269LeuMet: 1.269 ± 0.17
4.909LeuAsn: 4.909 ± 0.323
2.887LeuPro: 2.887 ± 0.239
2.721LeuGln: 2.721 ± 0.244
4.063LeuArg: 4.063 ± 0.28
4.872LeuSer: 4.872 ± 0.352
5.001LeuThr: 5.001 ± 0.411
4.358LeuVal: 4.358 ± 0.292
0.883LeuTrp: 0.883 ± 0.201
3.089LeuTyr: 3.089 ± 0.237
0.0LeuXaa: 0.0 ± 0.0
Met
1.379MetAla: 1.379 ± 0.202
0.202MetCys: 0.202 ± 0.055
1.14MetAsp: 1.14 ± 0.197
1.103MetGlu: 1.103 ± 0.184
0.993MetPhe: 0.993 ± 0.152
0.919MetGly: 0.919 ± 0.153
0.313MetHis: 0.313 ± 0.084
1.177MetIle: 1.177 ± 0.161
1.747MetLys: 1.747 ± 0.242
1.269MetLeu: 1.269 ± 0.211
0.57MetMet: 0.57 ± 0.125
1.397MetAsn: 1.397 ± 0.189
0.993MetPro: 0.993 ± 0.156
0.772MetGln: 0.772 ± 0.145
0.827MetArg: 0.827 ± 0.112
1.581MetSer: 1.581 ± 0.183
1.342MetThr: 1.342 ± 0.188
1.214MetVal: 1.214 ± 0.175
0.257MetTrp: 0.257 ± 0.084
0.809MetTyr: 0.809 ± 0.138
0.0MetXaa: 0.0 ± 0.0
Asn
3.126AsnAla: 3.126 ± 0.335
0.552AsnCys: 0.552 ± 0.112
3.622AsnAsp: 3.622 ± 0.306
3.163AsnGlu: 3.163 ± 0.204
2.923AsnPhe: 2.923 ± 0.23
4.597AsnGly: 4.597 ± 0.482
0.993AsnHis: 0.993 ± 0.131
4.211AsnIle: 4.211 ± 0.353
2.96AsnLys: 2.96 ± 0.249
3.898AsnLeu: 3.898 ± 0.241
0.974AsnMet: 0.974 ± 0.152
3.291AsnAsn: 3.291 ± 0.234
3.144AsnPro: 3.144 ± 0.3
1.949AsnGln: 1.949 ± 0.178
2.887AsnArg: 2.887 ± 0.268
4.394AsnSer: 4.394 ± 0.391
3.475AsnThr: 3.475 ± 0.413
4.082AsnVal: 4.082 ± 0.408
0.68AsnTrp: 0.68 ± 0.114
2.464AsnTyr: 2.464 ± 0.227
0.0AsnXaa: 0.0 ± 0.0
Pro
2.133ProAla: 2.133 ± 0.222
0.313ProCys: 0.313 ± 0.078
2.684ProAsp: 2.684 ± 0.278
3.071ProGlu: 3.071 ± 0.29
1.581ProPhe: 1.581 ± 0.186
3.107ProGly: 3.107 ± 0.286
0.791ProHis: 0.791 ± 0.148
2.39ProIle: 2.39 ± 0.235
2.188ProLys: 2.188 ± 0.207
2.574ProLeu: 2.574 ± 0.263
0.625ProMet: 0.625 ± 0.115
2.629ProAsn: 2.629 ± 0.232
2.151ProPro: 2.151 ± 0.328
1.434ProGln: 1.434 ± 0.16
1.6ProArg: 1.6 ± 0.18
2.85ProSer: 2.85 ± 0.247
3.99ProThr: 3.99 ± 0.514
2.666ProVal: 2.666 ± 0.235
0.46ProTrp: 0.46 ± 0.101
1.434ProTyr: 1.434 ± 0.166
0.0ProXaa: 0.0 ± 0.0
Gln
1.857GlnAla: 1.857 ± 0.184
0.276GlnCys: 0.276 ± 0.071
1.765GlnAsp: 1.765 ± 0.19
2.721GlnGlu: 2.721 ± 0.259
2.023GlnPhe: 2.023 ± 0.222
2.427GlnGly: 2.427 ± 0.245
0.533GlnHis: 0.533 ± 0.108
2.813GlnIle: 2.813 ± 0.258
2.482GlnLys: 2.482 ± 0.323
3.089GlnLeu: 3.089 ± 0.262
0.919GlnMet: 0.919 ± 0.149
2.17GlnAsn: 2.17 ± 0.234
1.379GlnPro: 1.379 ± 0.188
1.544GlnGln: 1.544 ± 0.148
1.949GlnArg: 1.949 ± 0.157
2.317GlnSer: 2.317 ± 0.202
1.82GlnThr: 1.82 ± 0.201
2.427GlnVal: 2.427 ± 0.245
0.515GlnTrp: 0.515 ± 0.099
1.692GlnTyr: 1.692 ± 0.177
0.0GlnXaa: 0.0 ± 0.0
Arg
2.409ArgAla: 2.409 ± 0.221
0.349ArgCys: 0.349 ± 0.078
2.666ArgAsp: 2.666 ± 0.21
2.703ArgGlu: 2.703 ± 0.28
2.28ArgPhe: 2.28 ± 0.187
3.089ArgGly: 3.089 ± 0.24
0.754ArgHis: 0.754 ± 0.133
2.868ArgIle: 2.868 ± 0.215
3.199ArgLys: 3.199 ± 0.394
3.696ArgLeu: 3.696 ± 0.312
0.993ArgMet: 0.993 ± 0.188
2.041ArgAsn: 2.041 ± 0.2
1.655ArgPro: 1.655 ± 0.189
1.747ArgGln: 1.747 ± 0.226
2.409ArgArg: 2.409 ± 0.31
3.199ArgSer: 3.199 ± 0.298
2.133ArgThr: 2.133 ± 0.269
2.776ArgVal: 2.776 ± 0.245
0.515ArgTrp: 0.515 ± 0.124
2.262ArgTyr: 2.262 ± 0.214
0.0ArgXaa: 0.0 ± 0.0
Ser
4.486SerAla: 4.486 ± 0.389
0.625SerCys: 0.625 ± 0.131
4.229SerAsp: 4.229 ± 0.389
3.733SerGlu: 3.733 ± 0.261
3.163SerPhe: 3.163 ± 0.232
6.288SerGly: 6.288 ± 0.518
1.269SerHis: 1.269 ± 0.158
4.505SerIle: 4.505 ± 0.374
4.155SerLys: 4.155 ± 0.343
5.148SerLeu: 5.148 ± 0.284
1.269SerMet: 1.269 ± 0.187
4.211SerAsn: 4.211 ± 0.515
2.593SerPro: 2.593 ± 0.297
2.427SerGln: 2.427 ± 0.213
2.813SerArg: 2.813 ± 0.244
4.909SerSer: 4.909 ± 0.459
5.498SerThr: 5.498 ± 0.595
4.854SerVal: 4.854 ± 0.364
0.827SerTrp: 0.827 ± 0.115
2.519SerTyr: 2.519 ± 0.198
0.0SerXaa: 0.0 ± 0.0
Thr
5.001ThrAla: 5.001 ± 0.548
0.405ThrCys: 0.405 ± 0.091
4.652ThrAsp: 4.652 ± 0.434
3.824ThrGlu: 3.824 ± 0.25
3.769ThrPhe: 3.769 ± 0.386
6.215ThrGly: 6.215 ± 1.01
1.324ThrHis: 1.324 ± 0.183
5.093ThrIle: 5.093 ± 0.497
3.071ThrLys: 3.071 ± 0.26
5.038ThrLeu: 5.038 ± 0.403
0.717ThrMet: 0.717 ± 0.116
3.714ThrAsn: 3.714 ± 0.495
3.181ThrPro: 3.181 ± 0.244
2.629ThrGln: 2.629 ± 0.254
2.482ThrArg: 2.482 ± 0.226
5.424ThrSer: 5.424 ± 0.49
6.509ThrThr: 6.509 ± 0.841
5.847ThrVal: 5.847 ± 0.621
0.735ThrTrp: 0.735 ± 0.135
2.317ThrTyr: 2.317 ± 0.221
0.0ThrXaa: 0.0 ± 0.0
Val
4.266ValAla: 4.266 ± 0.323
0.754ValCys: 0.754 ± 0.119
5.222ValAsp: 5.222 ± 0.326
4.486ValGlu: 4.486 ± 0.263
2.923ValPhe: 2.923 ± 0.283
6.343ValGly: 6.343 ± 0.638
0.919ValHis: 0.919 ± 0.144
4.027ValIle: 4.027 ± 0.288
4.082ValLys: 4.082 ± 0.348
4.027ValLeu: 4.027 ± 0.251
1.379ValMet: 1.379 ± 0.198
3.88ValAsn: 3.88 ± 0.276
2.758ValPro: 2.758 ± 0.191
2.298ValGln: 2.298 ± 0.163
2.997ValArg: 2.997 ± 0.249
5.461ValSer: 5.461 ± 0.62
5.553ValThr: 5.553 ± 0.559
4.909ValVal: 4.909 ± 0.385
0.478ValTrp: 0.478 ± 0.107
2.758ValTyr: 2.758 ± 0.213
0.0ValXaa: 0.0 ± 0.0
Trp
0.625TrpAla: 0.625 ± 0.12
0.239TrpCys: 0.239 ± 0.06
0.68TrpAsp: 0.68 ± 0.134
0.956TrpGlu: 0.956 ± 0.183
0.515TrpPhe: 0.515 ± 0.119
0.809TrpGly: 0.809 ± 0.146
0.257TrpHis: 0.257 ± 0.073
0.883TrpIle: 0.883 ± 0.137
0.754TrpLys: 0.754 ± 0.12
0.809TrpLeu: 0.809 ± 0.177
0.276TrpMet: 0.276 ± 0.069
0.68TrpAsn: 0.68 ± 0.11
0.386TrpPro: 0.386 ± 0.09
0.405TrpGln: 0.405 ± 0.084
0.699TrpArg: 0.699 ± 0.12
0.699TrpSer: 0.699 ± 0.15
0.827TrpThr: 0.827 ± 0.142
0.809TrpVal: 0.809 ± 0.136
0.239TrpTrp: 0.239 ± 0.076
0.588TrpTyr: 0.588 ± 0.115
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.023TyrAla: 2.023 ± 0.178
0.478TyrCys: 0.478 ± 0.136
3.972TyrAsp: 3.972 ± 0.274
2.703TyrGlu: 2.703 ± 0.264
1.839TyrPhe: 1.839 ± 0.185
2.997TyrGly: 2.997 ± 0.259
0.754TyrHis: 0.754 ± 0.152
2.188TyrIle: 2.188 ± 0.189
2.666TyrLys: 2.666 ± 0.291
3.383TyrLeu: 3.383 ± 0.292
0.846TyrMet: 0.846 ± 0.14
2.519TyrAsn: 2.519 ± 0.266
1.544TyrPro: 1.544 ± 0.191
1.636TyrGln: 1.636 ± 0.192
1.673TyrArg: 1.673 ± 0.196
2.979TyrSer: 2.979 ± 0.244
2.482TyrThr: 2.482 ± 0.216
3.034TyrVal: 3.034 ± 0.235
0.607TyrTrp: 0.607 ± 0.131
1.949TyrTyr: 1.949 ± 0.176
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 229 proteins (54388 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski