Amino acid dipepetide frequency for Bacillus phage vB_BpuM-BpSp

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.821AlaAla: 0.821 ± 0.133
0.077AlaCys: 0.077 ± 0.031
1.886AlaAsp: 1.886 ± 0.198
1.707AlaGlu: 1.707 ± 0.191
1.399AlaPhe: 1.399 ± 0.152
1.36AlaGly: 1.36 ± 0.21
0.398AlaHis: 0.398 ± 0.098
2.823AlaIle: 2.823 ± 0.181
3.092AlaLys: 3.092 ± 0.264
2.9AlaLeu: 2.9 ± 0.245
0.462AlaMet: 0.462 ± 0.078
2.104AlaAsn: 2.104 ± 0.198
0.475AlaPro: 0.475 ± 0.087
0.526AlaGln: 0.526 ± 0.09
1.104AlaArg: 1.104 ± 0.139
2.284AlaSer: 2.284 ± 0.24
1.578AlaThr: 1.578 ± 0.164
1.835AlaVal: 1.835 ± 0.17
0.192AlaTrp: 0.192 ± 0.056
1.142AlaTyr: 1.142 ± 0.128
0.0AlaXaa: 0.0 ± 0.0
Cys
0.167CysAla: 0.167 ± 0.045
0.026CysCys: 0.026 ± 0.017
0.231CysAsp: 0.231 ± 0.053
0.372CysGlu: 0.372 ± 0.077
0.372CysPhe: 0.372 ± 0.06
0.192CysGly: 0.192 ± 0.046
0.103CysHis: 0.103 ± 0.037
0.539CysIle: 0.539 ± 0.082
0.642CysLys: 0.642 ± 0.105
0.385CysLeu: 0.385 ± 0.063
0.103CysMet: 0.103 ± 0.036
0.346CysAsn: 0.346 ± 0.07
0.205CysPro: 0.205 ± 0.062
0.269CysGln: 0.269 ± 0.056
0.205CysArg: 0.205 ± 0.05
0.308CysSer: 0.308 ± 0.07
0.218CysThr: 0.218 ± 0.047
0.218CysVal: 0.218 ± 0.053
0.013CysTrp: 0.013 ± 0.012
0.269CysTyr: 0.269 ± 0.059
0.0CysXaa: 0.0 ± 0.0
Asp
1.565AspAla: 1.565 ± 0.124
0.423AspCys: 0.423 ± 0.088
4.145AspAsp: 4.145 ± 0.269
5.402AspGlu: 5.402 ± 0.325
4.132AspPhe: 4.132 ± 0.196
2.951AspGly: 2.951 ± 0.215
0.77AspHis: 0.77 ± 0.105
7.378AspIle: 7.378 ± 0.323
6.955AspLys: 6.955 ± 0.313
7.327AspLeu: 7.327 ± 0.286
1.193AspMet: 1.193 ± 0.138
5.453AspAsn: 5.453 ± 0.292
1.796AspPro: 1.796 ± 0.194
1.758AspGln: 1.758 ± 0.15
2.451AspArg: 2.451 ± 0.168
4.388AspSer: 4.388 ± 0.273
2.733AspThr: 2.733 ± 0.183
2.938AspVal: 2.938 ± 0.204
0.808AspTrp: 0.808 ± 0.138
3.875AspTyr: 3.875 ± 0.228
0.0AspXaa: 0.0 ± 0.0
Glu
2.117GluAla: 2.117 ± 0.18
0.436GluCys: 0.436 ± 0.084
5.736GluAsp: 5.736 ± 0.335
9.585GluGlu: 9.585 ± 0.523
4.773GluPhe: 4.773 ± 0.31
2.759GluGly: 2.759 ± 0.217
0.873GluHis: 0.873 ± 0.124
8.726GluIle: 8.726 ± 0.302
9.778GluLys: 9.778 ± 0.471
8.418GluLeu: 8.418 ± 0.414
1.694GluMet: 1.694 ± 0.163
7.327GluAsn: 7.327 ± 0.407
1.168GluPro: 1.168 ± 0.125
1.501GluGln: 1.501 ± 0.127
3.105GluArg: 3.105 ± 0.232
4.966GluSer: 4.966 ± 0.249
3.4GluThr: 3.4 ± 0.212
4.08GluVal: 4.08 ± 0.235
0.539GluTrp: 0.539 ± 0.076
4.145GluTyr: 4.145 ± 0.26
0.0GluXaa: 0.0 ± 0.0
Phe
1.488PheAla: 1.488 ± 0.152
0.308PheCys: 0.308 ± 0.069
3.516PheAsp: 3.516 ± 0.239
3.054PheGlu: 3.054 ± 0.209
2.63PhePhe: 2.63 ± 0.187
1.822PheGly: 1.822 ± 0.201
0.667PheHis: 0.667 ± 0.097
6.749PheIle: 6.749 ± 0.372
5.146PheLys: 5.146 ± 0.218
5.069PheLeu: 5.069 ± 0.282
1.078PheMet: 1.078 ± 0.132
5.094PheAsn: 5.094 ± 0.295
1.193PhePro: 1.193 ± 0.13
1.155PheGln: 1.155 ± 0.116
1.63PheArg: 1.63 ± 0.131
3.952PheSer: 3.952 ± 0.222
2.4PheThr: 2.4 ± 0.202
2.015PheVal: 2.015 ± 0.174
0.334PheTrp: 0.334 ± 0.064
2.643PheTyr: 2.643 ± 0.191
0.0PheXaa: 0.0 ± 0.0
Gly
1.181GlyAla: 1.181 ± 0.211
0.192GlyCys: 0.192 ± 0.045
2.233GlyAsp: 2.233 ± 0.171
2.81GlyGlu: 2.81 ± 0.186
2.117GlyPhe: 2.117 ± 0.177
1.835GlyGly: 1.835 ± 0.312
0.526GlyHis: 0.526 ± 0.084
3.567GlyIle: 3.567 ± 0.237
4.299GlyLys: 4.299 ± 0.3
3.529GlyLeu: 3.529 ± 0.275
0.744GlyMet: 0.744 ± 0.119
3.439GlyAsn: 3.439 ± 0.213
0.423GlyPro: 0.423 ± 0.063
0.95GlyGln: 0.95 ± 0.128
1.488GlyArg: 1.488 ± 0.146
3.118GlySer: 3.118 ± 0.269
1.899GlyThr: 1.899 ± 0.167
1.848GlyVal: 1.848 ± 0.168
0.295GlyTrp: 0.295 ± 0.056
1.848GlyTyr: 1.848 ± 0.15
0.0GlyXaa: 0.0 ± 0.0
His
0.398HisAla: 0.398 ± 0.079
0.128HisCys: 0.128 ± 0.038
0.616HisAsp: 0.616 ± 0.077
0.86HisGlu: 0.86 ± 0.134
0.796HisPhe: 0.796 ± 0.081
0.436HisGly: 0.436 ± 0.079
0.321HisHis: 0.321 ± 0.066
1.463HisIle: 1.463 ± 0.154
1.309HisLys: 1.309 ± 0.118
1.45HisLeu: 1.45 ± 0.129
0.244HisMet: 0.244 ± 0.062
0.757HisAsn: 0.757 ± 0.106
0.629HisPro: 0.629 ± 0.122
0.59HisGln: 0.59 ± 0.097
0.513HisArg: 0.513 ± 0.076
0.924HisSer: 0.924 ± 0.088
0.449HisThr: 0.449 ± 0.08
0.744HisVal: 0.744 ± 0.106
0.192HisTrp: 0.192 ± 0.048
0.808HisTyr: 0.808 ± 0.1
0.0HisXaa: 0.0 ± 0.0
Ile
2.695IleAla: 2.695 ± 0.174
0.462IleCys: 0.462 ± 0.085
7.263IleAsp: 7.263 ± 0.353
9.957IleGlu: 9.957 ± 0.492
4.119IlePhe: 4.119 ± 0.207
3.272IleGly: 3.272 ± 0.21
1.232IleHis: 1.232 ± 0.139
8.636IleIle: 8.636 ± 0.438
11.651IleLys: 11.651 ± 0.439
7.93IleLeu: 7.93 ± 0.405
2.156IleMet: 2.156 ± 0.163
9.701IleAsn: 9.701 ± 0.478
2.605IlePro: 2.605 ± 0.247
2.027IleGln: 2.027 ± 0.157
3.49IleArg: 3.49 ± 0.209
7.468IleSer: 7.468 ± 0.333
4.889IleThr: 4.889 ± 0.323
4.119IleVal: 4.119 ± 0.199
0.398IleTrp: 0.398 ± 0.063
4.337IleTyr: 4.337 ± 0.303
0.0IleXaa: 0.0 ± 0.0
Lys
2.759LysAla: 2.759 ± 0.23
0.539LysCys: 0.539 ± 0.096
8.469LysAsp: 8.469 ± 0.326
11.022LysGlu: 11.022 ± 0.414
5.633LysPhe: 5.633 ± 0.291
3.939LysGly: 3.939 ± 0.227
1.578LysHis: 1.578 ± 0.173
10.176LysIle: 10.176 ± 0.444
10.483LysLys: 10.483 ± 0.462
9.957LysLeu: 9.957 ± 0.473
2.528LysMet: 2.528 ± 0.186
9.239LysAsn: 9.239 ± 0.369
1.886LysPro: 1.886 ± 0.211
1.873LysGln: 1.873 ± 0.155
3.721LysArg: 3.721 ± 0.231
6.403LysSer: 6.403 ± 0.327
4.555LysThr: 4.555 ± 0.258
5.351LysVal: 5.351 ± 0.332
0.603LysTrp: 0.603 ± 0.101
6.185LysTyr: 6.185 ± 0.347
0.0LysXaa: 0.0 ± 0.0
Leu
2.707LeuAla: 2.707 ± 0.238
0.436LeuCys: 0.436 ± 0.079
7.096LeuAsp: 7.096 ± 0.304
8.238LeuGlu: 8.238 ± 0.446
4.696LeuPhe: 4.696 ± 0.285
3.49LeuGly: 3.49 ± 0.373
1.168LeuHis: 1.168 ± 0.122
8.61LeuIle: 8.61 ± 0.478
10.65LeuLys: 10.65 ± 0.441
8.174LeuLeu: 8.174 ± 0.431
2.451LeuMet: 2.451 ± 0.169
8.905LeuAsn: 8.905 ± 0.357
2.207LeuPro: 2.207 ± 0.175
1.514LeuGln: 1.514 ± 0.149
3.118LeuArg: 3.118 ± 0.216
7.622LeuSer: 7.622 ± 0.353
4.26LeuThr: 4.26 ± 0.241
3.991LeuVal: 3.991 ± 0.199
0.398LeuTrp: 0.398 ± 0.075
3.631LeuTyr: 3.631 ± 0.233
0.0LeuXaa: 0.0 ± 0.0
Met
0.706MetAla: 0.706 ± 0.106
0.115MetCys: 0.115 ± 0.04
1.54MetAsp: 1.54 ± 0.167
2.066MetGlu: 2.066 ± 0.161
0.693MetPhe: 0.693 ± 0.095
0.757MetGly: 0.757 ± 0.1
0.346MetHis: 0.346 ± 0.061
1.912MetIle: 1.912 ± 0.157
2.194MetLys: 2.194 ± 0.168
1.463MetLeu: 1.463 ± 0.136
0.5MetMet: 0.5 ± 0.08
1.63MetAsn: 1.63 ± 0.164
0.513MetPro: 0.513 ± 0.079
0.436MetGln: 0.436 ± 0.077
0.783MetArg: 0.783 ± 0.101
1.784MetSer: 1.784 ± 0.152
0.86MetThr: 0.86 ± 0.105
1.193MetVal: 1.193 ± 0.129
0.103MetTrp: 0.103 ± 0.035
0.988MetTyr: 0.988 ± 0.121
0.0MetXaa: 0.0 ± 0.0
Asn
2.079AsnAla: 2.079 ± 0.165
0.372AsnCys: 0.372 ± 0.08
5.235AsnAsp: 5.235 ± 0.253
6.814AsnGlu: 6.814 ± 0.339
4.684AsnPhe: 4.684 ± 0.302
3.696AsnGly: 3.696 ± 0.23
1.399AsnHis: 1.399 ± 0.154
8.995AsnIle: 8.995 ± 0.444
9.649AsnLys: 9.649 ± 0.43
7.84AsnLeu: 7.84 ± 0.299
1.681AsnMet: 1.681 ± 0.148
7.763AsnAsn: 7.763 ± 0.38
2.271AsnPro: 2.271 ± 0.201
2.066AsnGln: 2.066 ± 0.171
3.259AsnArg: 3.259 ± 0.233
5.851AsnSer: 5.851 ± 0.314
3.785AsnThr: 3.785 ± 0.253
3.875AsnVal: 3.875 ± 0.228
0.95AsnTrp: 0.95 ± 0.152
4.709AsnTyr: 4.709 ± 0.296
0.0AsnXaa: 0.0 ± 0.0
Pro
0.885ProAla: 0.885 ± 0.123
0.103ProCys: 0.103 ± 0.039
1.848ProAsp: 1.848 ± 0.16
1.861ProGlu: 1.861 ± 0.184
1.36ProPhe: 1.36 ± 0.143
0.334ProGly: 0.334 ± 0.059
0.295ProHis: 0.295 ± 0.067
2.04ProIle: 2.04 ± 0.206
2.169ProLys: 2.169 ± 0.163
1.886ProLeu: 1.886 ± 0.15
0.449ProMet: 0.449 ± 0.078
1.912ProAsn: 1.912 ± 0.145
0.565ProPro: 0.565 ± 0.107
0.436ProGln: 0.436 ± 0.083
0.654ProArg: 0.654 ± 0.091
1.835ProSer: 1.835 ± 0.185
1.168ProThr: 1.168 ± 0.15
1.463ProVal: 1.463 ± 0.137
0.051ProTrp: 0.051 ± 0.021
1.283ProTyr: 1.283 ± 0.131
0.0ProXaa: 0.0 ± 0.0
Gln
0.719GlnAla: 0.719 ± 0.092
0.128GlnCys: 0.128 ± 0.04
1.476GlnAsp: 1.476 ± 0.159
1.989GlnGlu: 1.989 ± 0.166
0.975GlnPhe: 0.975 ± 0.099
0.744GlnGly: 0.744 ± 0.124
0.282GlnHis: 0.282 ± 0.07
1.873GlnIle: 1.873 ± 0.154
2.707GlnLys: 2.707 ± 0.236
2.002GlnLeu: 2.002 ± 0.135
0.475GlnMet: 0.475 ± 0.071
1.655GlnAsn: 1.655 ± 0.123
0.5GlnPro: 0.5 ± 0.079
0.603GlnGln: 0.603 ± 0.098
0.757GlnArg: 0.757 ± 0.097
1.553GlnSer: 1.553 ± 0.143
1.116GlnThr: 1.116 ± 0.124
1.258GlnVal: 1.258 ± 0.132
0.192GlnTrp: 0.192 ± 0.053
1.155GlnTyr: 1.155 ± 0.116
0.0GlnXaa: 0.0 ± 0.0
Arg
1.193ArgAla: 1.193 ± 0.119
0.154ArgCys: 0.154 ± 0.042
2.695ArgAsp: 2.695 ± 0.178
2.964ArgGlu: 2.964 ± 0.204
2.117ArgPhe: 2.117 ± 0.174
1.527ArgGly: 1.527 ± 0.15
0.552ArgHis: 0.552 ± 0.096
3.336ArgIle: 3.336 ± 0.219
3.542ArgLys: 3.542 ± 0.213
3.567ArgLeu: 3.567 ± 0.247
0.706ArgMet: 0.706 ± 0.09
2.682ArgAsn: 2.682 ± 0.193
0.642ArgPro: 0.642 ± 0.095
0.783ArgGln: 0.783 ± 0.105
1.219ArgArg: 1.219 ± 0.138
2.143ArgSer: 2.143 ± 0.179
1.655ArgThr: 1.655 ± 0.143
1.873ArgVal: 1.873 ± 0.157
0.218ArgTrp: 0.218 ± 0.043
1.745ArgTyr: 1.745 ± 0.154
0.0ArgXaa: 0.0 ± 0.0
Ser
2.002SerAla: 2.002 ± 0.22
0.385SerCys: 0.385 ± 0.065
4.491SerAsp: 4.491 ± 0.264
5.492SerGlu: 5.492 ± 0.353
4.029SerPhe: 4.029 ± 0.249
3.169SerGly: 3.169 ± 0.272
0.988SerHis: 0.988 ± 0.121
7.147SerIle: 7.147 ± 0.294
7.378SerLys: 7.378 ± 0.33
7.173SerLeu: 7.173 ± 0.28
1.245SerMet: 1.245 ± 0.121
6.198SerAsn: 6.198 ± 0.326
1.54SerPro: 1.54 ± 0.128
1.527SerGln: 1.527 ± 0.163
2.258SerArg: 2.258 ± 0.174
5.287SerSer: 5.287 ± 0.454
3.426SerThr: 3.426 ± 0.219
3.4SerVal: 3.4 ± 0.254
0.462SerTrp: 0.462 ± 0.081
3.644SerTyr: 3.644 ± 0.233
0.0SerXaa: 0.0 ± 0.0
Thr
1.578ThrAla: 1.578 ± 0.203
0.231ThrCys: 0.231 ± 0.058
2.964ThrAsp: 2.964 ± 0.232
3.234ThrGlu: 3.234 ± 0.189
2.297ThrPhe: 2.297 ± 0.159
1.963ThrGly: 1.963 ± 0.186
0.706ThrHis: 0.706 ± 0.119
5.261ThrIle: 5.261 ± 0.273
4.453ThrLys: 4.453 ± 0.256
4.838ThrLeu: 4.838 ± 0.236
0.783ThrMet: 0.783 ± 0.095
3.298ThrAsn: 3.298 ± 0.177
1.334ThrPro: 1.334 ± 0.132
1.039ThrGln: 1.039 ± 0.145
1.642ThrArg: 1.642 ± 0.16
3.118ThrSer: 3.118 ± 0.221
2.361ThrThr: 2.361 ± 0.245
2.823ThrVal: 2.823 ± 0.218
0.411ThrTrp: 0.411 ± 0.068
2.22ThrTyr: 2.22 ± 0.178
0.0ThrXaa: 0.0 ± 0.0
Val
1.476ValAla: 1.476 ± 0.138
0.295ValCys: 0.295 ± 0.067
3.105ValAsp: 3.105 ± 0.21
3.606ValGlu: 3.606 ± 0.215
2.284ValPhe: 2.284 ± 0.166
1.95ValGly: 1.95 ± 0.175
0.59ValHis: 0.59 ± 0.088
4.209ValIle: 4.209 ± 0.243
5.107ValLys: 5.107 ± 0.287
4.363ValLeu: 4.363 ± 0.226
1.155ValMet: 1.155 ± 0.125
3.862ValAsn: 3.862 ± 0.214
1.309ValPro: 1.309 ± 0.169
1.052ValGln: 1.052 ± 0.132
1.63ValArg: 1.63 ± 0.165
4.003ValSer: 4.003 ± 0.299
2.592ValThr: 2.592 ± 0.201
2.72ValVal: 2.72 ± 0.222
0.282ValTrp: 0.282 ± 0.05
2.4ValTyr: 2.4 ± 0.179
0.0ValXaa: 0.0 ± 0.0
Trp
0.167TrpAla: 0.167 ± 0.036
0.051TrpCys: 0.051 ± 0.022
0.616TrpAsp: 0.616 ± 0.089
0.757TrpGlu: 0.757 ± 0.106
0.282TrpPhe: 0.282 ± 0.062
0.244TrpGly: 0.244 ± 0.053
0.064TrpHis: 0.064 ± 0.026
0.411TrpIle: 0.411 ± 0.068
0.616TrpLys: 0.616 ± 0.108
0.5TrpLeu: 0.5 ± 0.068
0.09TrpMet: 0.09 ± 0.033
0.796TrpAsn: 0.796 ± 0.135
0.0TrpPro: 0.0 ± 0.0
0.231TrpGln: 0.231 ± 0.059
0.257TrpArg: 0.257 ± 0.058
0.552TrpSer: 0.552 ± 0.075
0.488TrpThr: 0.488 ± 0.114
0.334TrpVal: 0.334 ± 0.065
0.103TrpTrp: 0.103 ± 0.035
0.334TrpTyr: 0.334 ± 0.062
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.476TyrAla: 1.476 ± 0.142
0.321TyrCys: 0.321 ± 0.073
3.285TyrAsp: 3.285 ± 0.25
3.336TyrGlu: 3.336 ± 0.188
2.554TyrPhe: 2.554 ± 0.2
1.899TyrGly: 1.899 ± 0.134
0.77TyrHis: 0.77 ± 0.107
4.555TyrIle: 4.555 ± 0.312
5.081TyrLys: 5.081 ± 0.317
4.594TyrLeu: 4.594 ± 0.3
0.834TyrMet: 0.834 ± 0.105
4.812TyrAsn: 4.812 ± 0.264
1.334TyrPro: 1.334 ± 0.166
1.822TyrGln: 1.822 ± 0.166
2.027TyrArg: 2.027 ± 0.163
3.683TyrSer: 3.683 ± 0.245
2.72TyrThr: 2.72 ± 0.203
1.912TyrVal: 1.912 ± 0.156
0.346TyrTrp: 0.346 ± 0.069
2.836TyrTyr: 2.836 ± 0.23
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 318 proteins (77933 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski