Ruegeria phage vB_RpoP-V13

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Rhodovirinae; Pomeroyivirus; Ruegeria virus V13

Average proteome isoelectric point is 5.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4QH08|A0A2Z4QH08_9CAUD Uncharacterized protein OS=Ruegeria phage vB_RpoP-V13 OX=2218612 GN=vBRpoPV13_40 PE=4 SV=1
MM1 pKa = 7.76AEE3 pKa = 4.07RR4 pKa = 11.84RR5 pKa = 11.84PLVLVSGEE13 pKa = 4.01IQEE16 pKa = 5.22LPTGDD21 pKa = 4.09TLPGAGGLQNNYY33 pKa = 10.09AATAAPAVTDD43 pKa = 4.44DD44 pKa = 4.9DD45 pKa = 4.92GSGYY49 pKa = 11.09AVGSRR54 pKa = 11.84WIDD57 pKa = 3.18VTNDD61 pKa = 3.01KK62 pKa = 11.1EE63 pKa = 4.64YY64 pKa = 11.03VCLDD68 pKa = 3.49ASTGAAVWTEE78 pKa = 3.9TTGAGGGGTTNNYY91 pKa = 10.23LIARR95 pKa = 11.84QEE97 pKa = 3.62FDD99 pKa = 3.46LAMGTTTSTSAYY111 pKa = 8.83AAKK114 pKa = 10.26GDD116 pKa = 3.61IMQAEE121 pKa = 4.37NDD123 pKa = 3.61FHH125 pKa = 7.81FLGVNTYY132 pKa = 9.4LASGQTVKK140 pKa = 11.05LMVLQVTHH148 pKa = 6.49VDD150 pKa = 3.25NGTATITSVLYY161 pKa = 10.75DD162 pKa = 3.56GTPVVISSTGNYY174 pKa = 9.45NFYY177 pKa = 8.8ITNGIAMTTGNRR189 pKa = 11.84YY190 pKa = 9.94LITFVRR196 pKa = 11.84TDD198 pKa = 3.06GSTTSALGIPFSGTTATDD216 pKa = 3.46EE217 pKa = 4.3NGNLSWRR224 pKa = 11.84NAIRR228 pKa = 11.84DD229 pKa = 3.82TVLDD233 pKa = 3.79YY234 pKa = 11.15AVSDD238 pKa = 3.68VVPAYY243 pKa = 10.94VSTTPVGMRR252 pKa = 11.84FYY254 pKa = 10.63TVDD257 pKa = 3.56NEE259 pKa = 4.11FLTILTTGPAGADD272 pKa = 3.47GADD275 pKa = 3.77GADD278 pKa = 3.69GLSAAISIVEE288 pKa = 4.19EE289 pKa = 4.09TTTARR294 pKa = 11.84VASNGDD300 pKa = 3.36LAGSVLVEE308 pKa = 4.19MNNAGAQTYY317 pKa = 7.31TVNSGLTGTEE327 pKa = 3.98PVTLIQKK334 pKa = 9.58GAGAVTVVAGASVTINSLDD353 pKa = 3.75GNLKK357 pKa = 10.46LAGQYY362 pKa = 11.14ASATLIPSGTDD373 pKa = 2.65NYY375 pKa = 10.63YY376 pKa = 11.03LVGALIAA383 pKa = 4.69

Molecular weight: 39.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4QGL5|A0A2Z4QGL5_9CAUD Uncharacterized protein OS=Ruegeria phage vB_RpoP-V13 OX=2218612 GN=vBRpoPV13_75 PE=4 SV=1
MM1 pKa = 7.3NWVLWFIFIPQIWSFMTYY19 pKa = 8.66TFTQRR24 pKa = 11.84KK25 pKa = 8.12PLSQFPRR32 pKa = 11.84LVGFNEE38 pKa = 4.58SVCRR42 pKa = 11.84RR43 pKa = 11.84VLDD46 pKa = 4.97SIWLPEE52 pKa = 4.03KK53 pKa = 10.16PQGIEE58 pKa = 3.63KK59 pKa = 9.8RR60 pKa = 11.84DD61 pKa = 3.67YY62 pKa = 11.23LVV64 pKa = 3.15

Molecular weight: 7.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

23347

37

3545

295.5

32.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.575 ± 0.397

0.737 ± 0.159

6.772 ± 0.156

7.054 ± 0.25

3.795 ± 0.162

7.132 ± 0.218

1.653 ± 0.162

5.401 ± 0.205

5.769 ± 0.317

7.808 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.187 ± 0.166

5.118 ± 0.194

4.125 ± 0.206

4.463 ± 0.267

4.656 ± 0.232

5.817 ± 0.15

6.378 ± 0.28

6.737 ± 0.211

1.473 ± 0.148

3.349 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski