Tobacco vein distorting virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus

Average proteome isoelectric point is 7.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2BJG5|B2BJG5_9LUTE Readthrough protein OS=Tobacco vein distorting virus OX=192203 GN=RT PE=3 SV=1
MM1 pKa = 6.92FRR3 pKa = 11.84EE4 pKa = 4.17AFGEE8 pKa = 3.94NGGKK12 pKa = 9.14NRR14 pKa = 11.84PFCYY18 pKa = 9.56RR19 pKa = 11.84KK20 pKa = 9.86EE21 pKa = 4.28SGGSISTEE29 pKa = 3.8SHH31 pKa = 6.08EE32 pKa = 4.34EE33 pKa = 3.58ASRR36 pKa = 11.84IPXAQTTAEE45 pKa = 3.76NFRR48 pKa = 11.84RR49 pKa = 11.84YY50 pKa = 9.96FEE52 pKa = 5.39GKK54 pKa = 10.13YY55 pKa = 9.53KK56 pKa = 10.3WEE58 pKa = 4.4VPASPQEE65 pKa = 3.76IPGFEE70 pKa = 4.24EE71 pKa = 4.98CGSLPQYY78 pKa = 8.33YY79 pKa = 9.71HH80 pKa = 7.18PKK82 pKa = 9.31QIKK85 pKa = 8.64GSQWGIQLIQEE96 pKa = 4.21HH97 pKa = 7.26PEE99 pKa = 3.96LGEE102 pKa = 4.1KK103 pKa = 10.61VSGFGWPAVGPQAEE117 pKa = 4.69VTSLTLQAEE126 pKa = 4.3RR127 pKa = 11.84WLQRR131 pKa = 11.84AQSAKK136 pKa = 9.98IPSSEE141 pKa = 3.75DD142 pKa = 3.04RR143 pKa = 11.84EE144 pKa = 4.04RR145 pKa = 11.84VINKK149 pKa = 7.96TVEE152 pKa = 3.93AYY154 pKa = 11.26SNVKK158 pKa = 9.07TFGPTATRR166 pKa = 11.84GNKK169 pKa = 9.98LEE171 pKa = 3.74WRR173 pKa = 11.84QFIEE177 pKa = 4.25DD178 pKa = 3.8FKK180 pKa = 11.45SAVFSLEE187 pKa = 3.4LDD189 pKa = 3.35AGIGVPYY196 pKa = 9.85IAYY199 pKa = 9.28GRR201 pKa = 11.84PTHH204 pKa = 6.38KK205 pKa = 10.41GWVEE209 pKa = 3.94DD210 pKa = 4.03PKK212 pKa = 11.09LLPVLARR219 pKa = 11.84LTFNRR224 pKa = 11.84LQKK227 pKa = 9.49MLEE230 pKa = 4.16VEE232 pKa = 4.18SSEE235 pKa = 3.98MSAEE239 pKa = 4.07EE240 pKa = 3.87LVQAGLCDD248 pKa = 4.92PIRR251 pKa = 11.84TFVKK255 pKa = 10.42RR256 pKa = 11.84EE257 pKa = 3.58PHH259 pKa = 5.86KK260 pKa = 10.47QSKK263 pKa = 10.11LDD265 pKa = 3.38EE266 pKa = 4.27GRR268 pKa = 11.84YY269 pKa = 8.96RR270 pKa = 11.84LIMSVSLVDD279 pKa = 3.45QLVARR284 pKa = 11.84VLFQNQNKK292 pKa = 9.66RR293 pKa = 11.84EE294 pKa = 3.96IALWRR299 pKa = 11.84ANPSKK304 pKa = 10.61PGFGLSTDD312 pKa = 3.63EE313 pKa = 4.0QVLEE317 pKa = 4.39FVQALAAQVEE327 pKa = 4.75VPPEE331 pKa = 3.73EE332 pKa = 5.59VITSWEE338 pKa = 4.24KK339 pKa = 10.58YY340 pKa = 9.87LVPTDD345 pKa = 3.67CSGFDD350 pKa = 3.21WSVAEE355 pKa = 4.57WMLHH359 pKa = 5.89DD360 pKa = 5.83DD361 pKa = 3.43MVVRR365 pKa = 11.84NKK367 pKa = 9.41LTLDD371 pKa = 4.03LNPTTEE377 pKa = 4.11KK378 pKa = 10.92LRR380 pKa = 11.84FAWLKK385 pKa = 10.59CISNSVLCLSDD396 pKa = 3.31GTLLXQRR403 pKa = 11.84VPGVQKK409 pKa = 10.45SGSYY413 pKa = 8.25NTSSSNSRR421 pKa = 11.84IRR423 pKa = 11.84VMAAYY428 pKa = 9.95HH429 pKa = 6.59CGADD433 pKa = 2.88WAMAMGDD440 pKa = 3.72DD441 pKa = 4.39ALEE444 pKa = 4.26SVNTNLEE451 pKa = 4.32VYY453 pKa = 10.11KK454 pKa = 10.98SLGFKK459 pKa = 10.72VEE461 pKa = 3.93VSGQLEE467 pKa = 4.4FCSHH471 pKa = 6.9IFRR474 pKa = 11.84APDD477 pKa = 2.96LALPVNEE484 pKa = 4.57RR485 pKa = 11.84KK486 pKa = 8.59MLYY489 pKa = 10.45KK490 pKa = 10.76LIFGYY495 pKa = 10.83NPGSGSLEE503 pKa = 4.12VISNYY508 pKa = 8.13XAACASVLNEE518 pKa = 4.41LRR520 pKa = 11.84HH521 pKa = 6.43DD522 pKa = 4.37PDD524 pKa = 4.09SVALLXXWLVHH535 pKa = 6.39PVLPQNDD542 pKa = 3.36

Molecular weight: 60.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2BJG7|B2BJG7_9LUTE Movement protein OS=Tobacco vein distorting virus OX=192203 GN=MP PE=3 SV=1
MM1 pKa = 6.74NTGGARR7 pKa = 11.84SNNGNGGSRR16 pKa = 11.84VSRR19 pKa = 11.84PRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84ARR25 pKa = 11.84SVRR28 pKa = 11.84PVVVVAPPRR37 pKa = 11.84GARR40 pKa = 11.84XRR42 pKa = 11.84TRR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84NGGRR51 pKa = 11.84NRR53 pKa = 11.84RR54 pKa = 11.84GRR56 pKa = 11.84NGVGGRR62 pKa = 11.84SSNSEE67 pKa = 3.69TFIFNKK73 pKa = 10.42DD74 pKa = 3.59SIKK77 pKa = 10.81DD78 pKa = 3.66SSSGSITFGPSLSEE92 pKa = 4.12SVALSGGVLKK102 pKa = 10.54AYY104 pKa = 10.22HH105 pKa = 6.68EE106 pKa = 4.5YY107 pKa = 10.51KK108 pKa = 9.32ITMVNIRR115 pKa = 11.84FISEE119 pKa = 4.02SSSTAEE125 pKa = 3.6GSIAYY130 pKa = 9.39EE131 pKa = 4.08LDD133 pKa = 3.42PHH135 pKa = 6.93CKK137 pKa = 10.08LSSLQSTLRR146 pKa = 11.84KK147 pKa = 9.77FPVTKK152 pKa = 10.36GGQATFRR159 pKa = 11.84AAQINGVEE167 pKa = 4.07WHH169 pKa = 7.1DD170 pKa = 3.55TTEE173 pKa = 3.94DD174 pKa = 3.47QFRR177 pKa = 11.84LLYY180 pKa = 10.2KK181 pKa = 10.92GNGTKK186 pKa = 10.41GVAAGFFQIRR196 pKa = 11.84YY197 pKa = 6.05TVQLHH202 pKa = 5.2NPKK205 pKa = 10.54

Molecular weight: 22.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2533

156

723

422.2

46.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.711 ± 0.315

1.303 ± 0.282

3.908 ± 0.772

6.514 ± 0.711

3.829 ± 0.365

7.58 ± 0.721

2.132 ± 0.276

3.79 ± 0.337

5.527 ± 0.658

8.725 ± 0.91

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.856 ± 0.229

4.501 ± 0.413

6.396 ± 0.547

4.224 ± 0.281

6.79 ± 1.151

9.988 ± 0.605

5.448 ± 0.523

6.04 ± 0.8

1.54 ± 0.344

2.487 ± 0.362
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski