Glycomyces sp. MMG10089

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Glycomycetales; Glycomycetaceae; Glycomyces; unclassified Glycomyces

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4346 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R7PWP9|A0A4R7PWP9_9ACTN MoxR-like ATPase OS=Glycomyces sp. MMG10089 OX=2135788 GN=C8R39_4135 PE=4 SV=1
MM1 pKa = 6.74QAPRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84LALTGVAVGALAAGGLTFVSANQALAEE36 pKa = 4.32EE37 pKa = 5.67GCQVDD42 pKa = 4.13YY43 pKa = 11.57NVISTWGDD51 pKa = 3.12GFQANVSITAGEE63 pKa = 4.84AINGWNLQWNFPSGTTVSSAWNVDD87 pKa = 2.84WSQSGTTFTGSDD99 pKa = 3.62VGWNASIASGQTTEE113 pKa = 3.78VFGFIGSGSSTAPAQFSINGDD134 pKa = 3.56VCNGQVDD141 pKa = 4.74PDD143 pKa = 4.1PTDD146 pKa = 4.5PDD148 pKa = 3.92PTDD151 pKa = 4.88PDD153 pKa = 3.98PTDD156 pKa = 4.57PDD158 pKa = 4.71PDD160 pKa = 4.44PGNPGDD166 pKa = 4.87KK167 pKa = 10.38VDD169 pKa = 3.79NPYY172 pKa = 10.9VGAQVYY178 pKa = 8.71VNPIWSANAAAEE190 pKa = 4.31PGGSAIANEE199 pKa = 4.2PTGVWLDD206 pKa = 3.58QTSAIYY212 pKa = 10.72GNGSPTTGSYY222 pKa = 11.26GLADD226 pKa = 4.18HH227 pKa = 7.16LDD229 pKa = 3.72EE230 pKa = 5.62AVSQGADD237 pKa = 4.06LIQVVIYY244 pKa = 9.21NLPGRR249 pKa = 11.84DD250 pKa = 3.5CAALASNGQLAPNEE264 pKa = 3.72INRR267 pKa = 11.84YY268 pKa = 5.96KK269 pKa = 10.31TEE271 pKa = 4.4YY272 pKa = 9.42IDD274 pKa = 4.45EE275 pKa = 4.57IVDD278 pKa = 3.51IMSDD282 pKa = 3.2PAYY285 pKa = 10.68ADD287 pKa = 3.42LRR289 pKa = 11.84IVNVIEE295 pKa = 4.65IDD297 pKa = 3.71SLPNLITNVSPRR309 pKa = 11.84EE310 pKa = 4.09TATDD314 pKa = 3.32TCDD317 pKa = 2.98TMLANGGYY325 pKa = 9.99VDD327 pKa = 4.02GVSYY331 pKa = 10.85AAAEE335 pKa = 4.27LGALPNTYY343 pKa = 10.68NYY345 pKa = 9.95IDD347 pKa = 4.45AGHH350 pKa = 6.89HH351 pKa = 5.7GWIGWGDD358 pKa = 4.18DD359 pKa = 3.33NPEE362 pKa = 3.82YY363 pKa = 11.38DD364 pKa = 3.7NFRR367 pKa = 11.84ASAALFGSLIGQNGMTADD385 pKa = 4.11DD386 pKa = 3.54VHH388 pKa = 7.93GFISNTANYY397 pKa = 10.08SVLEE401 pKa = 4.0EE402 pKa = 4.88PYY404 pKa = 10.16WDD406 pKa = 3.13VDD408 pKa = 3.86QNVGGQALNQNPDD421 pKa = 3.97LPWVDD426 pKa = 2.72WNDD429 pKa = 3.4YY430 pKa = 11.1NNEE433 pKa = 3.89KK434 pKa = 10.92DD435 pKa = 3.33FTEE438 pKa = 4.13ALRR441 pKa = 11.84TEE443 pKa = 5.27LIANGFNSDD452 pKa = 2.87IGMLIDD458 pKa = 3.66TSRR461 pKa = 11.84NGWGGSYY468 pKa = 10.31RR469 pKa = 11.84PTGPSTSNDD478 pKa = 2.71PVTFVDD484 pKa = 4.93EE485 pKa = 4.2SRR487 pKa = 11.84IDD489 pKa = 3.47QRR491 pKa = 11.84IQKK494 pKa = 10.01GNWCNQAGAGLGEE507 pKa = 4.31RR508 pKa = 11.84PQVQPEE514 pKa = 4.0PWIDD518 pKa = 3.13AYY520 pKa = 11.47VWIKK524 pKa = 10.65PPGEE528 pKa = 4.04SDD530 pKa = 3.36GSSEE534 pKa = 6.07LIDD537 pKa = 3.69NDD539 pKa = 3.3EE540 pKa = 4.37GKK542 pKa = 10.93GFDD545 pKa = 3.89EE546 pKa = 4.9MCDD549 pKa = 3.32PTYY552 pKa = 11.1GGNPRR557 pKa = 11.84NGNNLSGARR566 pKa = 11.84GDD568 pKa = 4.01MPVSGHH574 pKa = 5.69WSSEE578 pKa = 3.76QFQEE582 pKa = 4.25LLQNAWPPINN592 pKa = 3.92

Molecular weight: 63.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V3F8E9|A0A4V3F8E9_9ACTN ATP-dependent Clp protease adapter protein ClpS OS=Glycomyces sp. MMG10089 OX=2135788 GN=clpS PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.7LLRR22 pKa = 11.84KK23 pKa = 7.95TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4346

0

4346

1448852

31

5306

333.4

35.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.809 ± 0.053

0.731 ± 0.011

6.263 ± 0.032

6.473 ± 0.031

3.096 ± 0.022

9.081 ± 0.039

2.122 ± 0.019

3.858 ± 0.026

2.136 ± 0.025

10.047 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.796 ± 0.015

2.062 ± 0.02

5.701 ± 0.037

2.683 ± 0.019

7.399 ± 0.043

5.092 ± 0.028

5.758 ± 0.025

8.084 ± 0.037

1.653 ± 0.019

2.155 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski