Amino acid dipepetide frequency for Arthrobacter phage Shoya

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.621AlaAla: 19.621 ± 1.679
0.761AlaCys: 0.761 ± 0.261
6.935AlaAsp: 6.935 ± 0.838
6.766AlaGlu: 6.766 ± 0.881
3.89AlaPhe: 3.89 ± 0.596
10.91AlaGly: 10.91 ± 1.217
1.691AlaHis: 1.691 ± 0.428
5.413AlaIle: 5.413 ± 0.897
6.089AlaLys: 6.089 ± 0.633
9.134AlaLeu: 9.134 ± 0.938
3.975AlaMet: 3.975 ± 0.544
3.552AlaAsn: 3.552 ± 0.552
6.343AlaPro: 6.343 ± 0.672
6.089AlaGln: 6.089 ± 0.835
6.766AlaArg: 6.766 ± 0.836
5.582AlaSer: 5.582 ± 0.691
6.85AlaThr: 6.85 ± 1.114
7.781AlaVal: 7.781 ± 0.962
3.721AlaTrp: 3.721 ± 0.589
2.622AlaTyr: 2.622 ± 0.583
0.0AlaXaa: 0.0 ± 0.0
Cys
0.93CysAla: 0.93 ± 0.346
0.085CysCys: 0.085 ± 0.091
0.169CysAsp: 0.169 ± 0.102
0.169CysGlu: 0.169 ± 0.102
0.169CysPhe: 0.169 ± 0.117
1.015CysGly: 1.015 ± 0.323
0.0CysHis: 0.0 ± 0.0
0.254CysIle: 0.254 ± 0.145
0.677CysLys: 0.677 ± 0.277
0.338CysLeu: 0.338 ± 0.194
0.085CysMet: 0.085 ± 0.073
0.338CysAsn: 0.338 ± 0.169
0.846CysPro: 0.846 ± 0.285
0.338CysGln: 0.338 ± 0.155
0.846CysArg: 0.846 ± 0.308
0.169CysSer: 0.169 ± 0.103
0.677CysThr: 0.677 ± 0.276
0.254CysVal: 0.254 ± 0.138
0.085CysTrp: 0.085 ± 0.079
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
6.766AspAla: 6.766 ± 0.734
0.254AspCys: 0.254 ± 0.13
4.06AspAsp: 4.06 ± 0.586
3.806AspGlu: 3.806 ± 0.586
1.269AspPhe: 1.269 ± 0.289
6.681AspGly: 6.681 ± 0.748
1.015AspHis: 1.015 ± 0.278
2.706AspIle: 2.706 ± 0.377
2.537AspLys: 2.537 ± 0.491
5.413AspLeu: 5.413 ± 0.646
1.099AspMet: 1.099 ± 0.344
1.269AspAsn: 1.269 ± 0.324
3.129AspPro: 3.129 ± 0.59
2.453AspGln: 2.453 ± 0.429
4.06AspArg: 4.06 ± 0.679
2.706AspSer: 2.706 ± 0.48
3.129AspThr: 3.129 ± 0.491
4.736AspVal: 4.736 ± 0.48
1.522AspTrp: 1.522 ± 0.293
1.269AspTyr: 1.269 ± 0.288
0.0AspXaa: 0.0 ± 0.0
Glu
8.965GluAla: 8.965 ± 0.892
0.592GluCys: 0.592 ± 0.223
3.045GluAsp: 3.045 ± 0.566
3.552GluGlu: 3.552 ± 0.653
2.03GluPhe: 2.03 ± 0.507
4.821GluGly: 4.821 ± 0.648
1.438GluHis: 1.438 ± 0.367
2.876GluIle: 2.876 ± 0.44
2.453GluLys: 2.453 ± 0.432
4.398GluLeu: 4.398 ± 0.717
1.269GluMet: 1.269 ± 0.301
1.691GluAsn: 1.691 ± 0.341
3.637GluPro: 3.637 ± 0.636
2.96GluGln: 2.96 ± 0.408
3.383GluArg: 3.383 ± 0.571
2.114GluSer: 2.114 ± 0.451
2.876GluThr: 2.876 ± 0.468
3.468GluVal: 3.468 ± 0.468
1.438GluTrp: 1.438 ± 0.354
1.522GluTyr: 1.522 ± 0.345
0.0GluXaa: 0.0 ± 0.0
Phe
3.89PheAla: 3.89 ± 0.52
0.338PheCys: 0.338 ± 0.158
1.861PheAsp: 1.861 ± 0.407
1.945PheGlu: 1.945 ± 0.418
1.099PhePhe: 1.099 ± 0.274
2.114PheGly: 2.114 ± 0.629
0.761PheHis: 0.761 ± 0.23
1.691PheIle: 1.691 ± 0.566
1.861PheLys: 1.861 ± 0.448
1.776PheLeu: 1.776 ± 0.367
0.592PheMet: 0.592 ± 0.197
0.846PheAsn: 0.846 ± 0.325
1.015PhePro: 1.015 ± 0.311
0.846PheGln: 0.846 ± 0.229
1.945PheArg: 1.945 ± 0.379
1.353PheSer: 1.353 ± 0.262
3.045PheThr: 3.045 ± 0.574
1.438PheVal: 1.438 ± 0.342
0.592PheTrp: 0.592 ± 0.266
0.761PheTyr: 0.761 ± 0.23
0.0PheXaa: 0.0 ± 0.0
Gly
10.318GlyAla: 10.318 ± 1.85
0.169GlyCys: 0.169 ± 0.12
4.905GlyAsp: 4.905 ± 0.405
4.482GlyGlu: 4.482 ± 0.525
2.537GlyPhe: 2.537 ± 0.693
6.85GlyGly: 6.85 ± 0.751
2.03GlyHis: 2.03 ± 0.541
3.468GlyIle: 3.468 ± 0.748
3.298GlyLys: 3.298 ± 0.55
7.273GlyLeu: 7.273 ± 0.875
2.03GlyMet: 2.03 ± 0.422
3.298GlyAsn: 3.298 ± 0.577
4.99GlyPro: 4.99 ± 0.884
2.96GlyGln: 2.96 ± 0.447
4.398GlyArg: 4.398 ± 0.62
4.821GlySer: 4.821 ± 0.575
6.258GlyThr: 6.258 ± 1.139
5.328GlyVal: 5.328 ± 0.86
1.438GlyTrp: 1.438 ± 0.319
3.468GlyTyr: 3.468 ± 0.631
0.0GlyXaa: 0.0 ± 0.0
His
1.861HisAla: 1.861 ± 0.374
0.254HisCys: 0.254 ± 0.193
0.93HisAsp: 0.93 ± 0.233
1.353HisGlu: 1.353 ± 0.346
0.592HisPhe: 0.592 ± 0.188
2.03HisGly: 2.03 ± 0.384
0.507HisHis: 0.507 ± 0.193
0.93HisIle: 0.93 ± 0.313
0.423HisLys: 0.423 ± 0.187
1.522HisLeu: 1.522 ± 0.4
0.338HisMet: 0.338 ± 0.143
0.677HisAsn: 0.677 ± 0.251
1.776HisPro: 1.776 ± 0.372
1.015HisGln: 1.015 ± 0.386
1.438HisArg: 1.438 ± 0.303
1.184HisSer: 1.184 ± 0.314
0.761HisThr: 0.761 ± 0.235
1.522HisVal: 1.522 ± 0.357
0.677HisTrp: 0.677 ± 0.202
0.423HisTyr: 0.423 ± 0.197
0.0HisXaa: 0.0 ± 0.0
Ile
4.652IleAla: 4.652 ± 0.64
0.338IleCys: 0.338 ± 0.178
3.383IleAsp: 3.383 ± 0.504
2.96IleGlu: 2.96 ± 0.479
1.015IlePhe: 1.015 ± 0.353
3.637IleGly: 3.637 ± 0.849
0.507IleHis: 0.507 ± 0.239
1.691IleIle: 1.691 ± 0.395
2.622IleLys: 2.622 ± 0.726
2.537IleLeu: 2.537 ± 0.406
1.184IleMet: 1.184 ± 0.343
1.184IleAsn: 1.184 ± 0.256
2.791IlePro: 2.791 ± 0.378
2.283IleGln: 2.283 ± 0.385
3.468IleArg: 3.468 ± 0.634
2.283IleSer: 2.283 ± 0.416
4.06IleThr: 4.06 ± 0.585
3.383IleVal: 3.383 ± 0.424
0.423IleTrp: 0.423 ± 0.176
0.677IleTyr: 0.677 ± 0.221
0.0IleXaa: 0.0 ± 0.0
Lys
6.766LysAla: 6.766 ± 0.776
0.085LysCys: 0.085 ± 0.08
3.214LysAsp: 3.214 ± 0.606
1.691LysGlu: 1.691 ± 0.407
1.184LysPhe: 1.184 ± 0.403
3.298LysGly: 3.298 ± 0.627
0.677LysHis: 0.677 ± 0.247
1.691LysIle: 1.691 ± 0.368
1.607LysLys: 1.607 ± 0.269
2.199LysLeu: 2.199 ± 0.407
0.93LysMet: 0.93 ± 0.3
1.269LysAsn: 1.269 ± 0.299
2.791LysPro: 2.791 ± 0.633
1.691LysGln: 1.691 ± 0.406
3.552LysArg: 3.552 ± 0.433
2.622LysSer: 2.622 ± 0.47
2.537LysThr: 2.537 ± 0.493
4.482LysVal: 4.482 ± 0.614
0.507LysTrp: 0.507 ± 0.203
1.099LysTyr: 1.099 ± 0.33
0.0LysXaa: 0.0 ± 0.0
Leu
8.542LeuAla: 8.542 ± 1.033
0.677LeuCys: 0.677 ± 0.242
4.99LeuAsp: 4.99 ± 0.794
3.637LeuGlu: 3.637 ± 0.505
2.114LeuPhe: 2.114 ± 0.361
5.582LeuGly: 5.582 ± 1.145
2.03LeuHis: 2.03 ± 0.362
3.637LeuIle: 3.637 ± 0.862
3.129LeuLys: 3.129 ± 0.507
5.159LeuLeu: 5.159 ± 0.651
1.776LeuMet: 1.776 ± 0.371
2.537LeuAsn: 2.537 ± 0.447
4.821LeuPro: 4.821 ± 0.821
2.453LeuGln: 2.453 ± 0.494
4.567LeuArg: 4.567 ± 0.727
4.398LeuSer: 4.398 ± 0.557
5.328LeuThr: 5.328 ± 0.779
6.258LeuVal: 6.258 ± 0.748
1.015LeuTrp: 1.015 ± 0.266
1.269LeuTyr: 1.269 ± 0.356
0.0LeuXaa: 0.0 ± 0.0
Met
3.214MetAla: 3.214 ± 0.436
0.085MetCys: 0.085 ± 0.093
1.099MetAsp: 1.099 ± 0.243
0.846MetGlu: 0.846 ± 0.274
0.507MetPhe: 0.507 ± 0.211
1.607MetGly: 1.607 ± 0.313
0.846MetHis: 0.846 ± 0.281
0.93MetIle: 0.93 ± 0.266
1.522MetLys: 1.522 ± 0.255
1.861MetLeu: 1.861 ± 0.472
0.338MetMet: 0.338 ± 0.159
0.761MetAsn: 0.761 ± 0.233
1.269MetPro: 1.269 ± 0.289
0.592MetGln: 0.592 ± 0.226
0.507MetArg: 0.507 ± 0.21
1.945MetSer: 1.945 ± 0.403
2.791MetThr: 2.791 ± 0.627
1.522MetVal: 1.522 ± 0.374
0.254MetTrp: 0.254 ± 0.154
0.169MetTyr: 0.169 ± 0.118
0.0MetXaa: 0.0 ± 0.0
Asn
3.89AsnAla: 3.89 ± 0.589
0.423AsnCys: 0.423 ± 0.174
1.438AsnAsp: 1.438 ± 0.355
1.945AsnGlu: 1.945 ± 0.338
0.677AsnPhe: 0.677 ± 0.234
3.637AsnGly: 3.637 ± 0.595
0.677AsnHis: 0.677 ± 0.308
1.776AsnIle: 1.776 ± 0.42
0.761AsnLys: 0.761 ± 0.266
2.453AsnLeu: 2.453 ± 0.429
0.592AsnMet: 0.592 ± 0.228
0.761AsnAsn: 0.761 ± 0.263
2.622AsnPro: 2.622 ± 0.442
0.846AsnGln: 0.846 ± 0.284
2.368AsnArg: 2.368 ± 0.452
1.945AsnSer: 1.945 ± 0.502
0.93AsnThr: 0.93 ± 0.252
2.791AsnVal: 2.791 ± 0.467
0.423AsnTrp: 0.423 ± 0.162
0.254AsnTyr: 0.254 ± 0.119
0.0AsnXaa: 0.0 ± 0.0
Pro
7.273ProAla: 7.273 ± 0.825
0.254ProCys: 0.254 ± 0.174
3.468ProAsp: 3.468 ± 0.492
4.398ProGlu: 4.398 ± 0.7
1.015ProPhe: 1.015 ± 0.267
5.92ProGly: 5.92 ± 0.635
0.846ProHis: 0.846 ± 0.22
2.283ProIle: 2.283 ± 0.423
1.945ProLys: 1.945 ± 0.476
4.06ProLeu: 4.06 ± 0.585
1.015ProMet: 1.015 ± 0.311
1.776ProAsn: 1.776 ± 0.44
3.468ProPro: 3.468 ± 0.701
1.607ProGln: 1.607 ± 0.342
3.383ProArg: 3.383 ± 0.626
2.876ProSer: 2.876 ± 0.433
4.482ProThr: 4.482 ± 0.586
5.666ProVal: 5.666 ± 0.97
1.184ProTrp: 1.184 ± 0.325
0.592ProTyr: 0.592 ± 0.187
0.0ProXaa: 0.0 ± 0.0
Gln
5.074GlnAla: 5.074 ± 0.594
0.254GlnCys: 0.254 ± 0.154
1.353GlnAsp: 1.353 ± 0.344
2.368GlnGlu: 2.368 ± 0.451
1.522GlnPhe: 1.522 ± 0.331
2.114GlnGly: 2.114 ± 0.352
0.761GlnHis: 0.761 ± 0.236
1.776GlnIle: 1.776 ± 0.314
0.93GlnLys: 0.93 ± 0.223
3.298GlnLeu: 3.298 ± 0.601
0.761GlnMet: 0.761 ± 0.246
0.93GlnAsn: 0.93 ± 0.375
2.622GlnPro: 2.622 ± 0.636
2.114GlnGln: 2.114 ± 0.513
2.622GlnArg: 2.622 ± 0.535
2.283GlnSer: 2.283 ± 0.48
2.114GlnThr: 2.114 ± 0.437
3.045GlnVal: 3.045 ± 0.413
1.438GlnTrp: 1.438 ± 0.385
1.269GlnTyr: 1.269 ± 0.289
0.0GlnXaa: 0.0 ± 0.0
Arg
7.358ArgAla: 7.358 ± 0.808
0.761ArgCys: 0.761 ± 0.248
3.298ArgAsp: 3.298 ± 0.525
3.552ArgGlu: 3.552 ± 0.511
2.114ArgPhe: 2.114 ± 0.387
4.313ArgGly: 4.313 ± 0.718
1.438ArgHis: 1.438 ± 0.401
2.706ArgIle: 2.706 ± 0.408
3.975ArgLys: 3.975 ± 0.66
4.229ArgLeu: 4.229 ± 0.752
1.691ArgMet: 1.691 ± 0.352
1.945ArgAsn: 1.945 ± 0.45
2.622ArgPro: 2.622 ± 0.44
2.199ArgGln: 2.199 ± 0.433
4.736ArgArg: 4.736 ± 0.723
3.89ArgSer: 3.89 ± 0.674
4.736ArgThr: 4.736 ± 0.675
4.736ArgVal: 4.736 ± 0.57
1.269ArgTrp: 1.269 ± 0.425
1.522ArgTyr: 1.522 ± 0.359
0.0ArgXaa: 0.0 ± 0.0
Ser
5.159SerAla: 5.159 ± 0.563
0.423SerCys: 0.423 ± 0.197
3.721SerAsp: 3.721 ± 0.609
3.975SerGlu: 3.975 ± 0.601
1.776SerPhe: 1.776 ± 0.463
5.413SerGly: 5.413 ± 0.727
0.93SerHis: 0.93 ± 0.242
2.283SerIle: 2.283 ± 0.316
1.776SerLys: 1.776 ± 0.41
3.129SerLeu: 3.129 ± 0.435
1.607SerMet: 1.607 ± 0.376
2.368SerAsn: 2.368 ± 0.497
2.791SerPro: 2.791 ± 0.523
1.438SerGln: 1.438 ± 0.374
3.214SerArg: 3.214 ± 0.452
2.622SerSer: 2.622 ± 0.471
3.298SerThr: 3.298 ± 0.56
4.567SerVal: 4.567 ± 0.647
1.269SerTrp: 1.269 ± 0.365
1.522SerTyr: 1.522 ± 0.398
0.0SerXaa: 0.0 ± 0.0
Thr
7.781ThrAla: 7.781 ± 0.787
0.423ThrCys: 0.423 ± 0.178
4.398ThrAsp: 4.398 ± 0.598
3.383ThrGlu: 3.383 ± 0.589
2.453ThrPhe: 2.453 ± 0.453
6.005ThrGly: 6.005 ± 0.777
1.522ThrHis: 1.522 ± 0.343
3.637ThrIle: 3.637 ± 0.632
2.622ThrLys: 2.622 ± 0.407
5.074ThrLeu: 5.074 ± 0.726
1.691ThrMet: 1.691 ± 0.395
1.945ThrAsn: 1.945 ± 0.556
4.398ThrPro: 4.398 ± 0.76
2.537ThrGln: 2.537 ± 0.374
4.06ThrArg: 4.06 ± 0.509
3.129ThrSer: 3.129 ± 0.542
5.666ThrThr: 5.666 ± 0.666
4.652ThrVal: 4.652 ± 0.603
1.015ThrTrp: 1.015 ± 0.228
2.03ThrTyr: 2.03 ± 0.487
0.0ThrXaa: 0.0 ± 0.0
Val
8.035ValAla: 8.035 ± 0.726
0.677ValCys: 0.677 ± 0.307
4.821ValAsp: 4.821 ± 0.658
4.821ValGlu: 4.821 ± 0.702
2.791ValPhe: 2.791 ± 1.066
4.482ValGly: 4.482 ± 0.726
1.776ValHis: 1.776 ± 0.432
3.975ValIle: 3.975 ± 0.743
3.975ValLys: 3.975 ± 0.592
5.497ValLeu: 5.497 ± 0.893
0.761ValMet: 0.761 ± 0.243
2.537ValAsn: 2.537 ± 0.399
3.637ValPro: 3.637 ± 0.487
2.283ValGln: 2.283 ± 0.411
3.975ValArg: 3.975 ± 0.478
5.244ValSer: 5.244 ± 0.81
6.089ValThr: 6.089 ± 0.912
4.736ValVal: 4.736 ± 0.878
1.691ValTrp: 1.691 ± 0.515
2.03ValTyr: 2.03 ± 0.546
0.0ValXaa: 0.0 ± 0.0
Trp
2.114TrpAla: 2.114 ± 0.466
0.169TrpCys: 0.169 ± 0.115
1.522TrpAsp: 1.522 ± 0.307
1.184TrpGlu: 1.184 ± 0.316
0.507TrpPhe: 0.507 ± 0.19
1.099TrpGly: 1.099 ± 0.303
0.507TrpHis: 0.507 ± 0.242
0.423TrpIle: 0.423 ± 0.175
1.015TrpLys: 1.015 ± 0.208
2.622TrpLeu: 2.622 ± 0.611
0.592TrpMet: 0.592 ± 0.226
1.269TrpAsn: 1.269 ± 0.298
1.099TrpPro: 1.099 ± 0.314
0.93TrpGln: 0.93 ± 0.287
1.353TrpArg: 1.353 ± 0.334
0.93TrpSer: 0.93 ± 0.265
1.099TrpThr: 1.099 ± 0.366
1.522TrpVal: 1.522 ± 0.342
0.507TrpTrp: 0.507 ± 0.189
0.338TrpTyr: 0.338 ± 0.176
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.283TyrAla: 2.283 ± 0.443
0.423TyrCys: 0.423 ± 0.237
1.269TyrAsp: 1.269 ± 0.349
1.691TyrGlu: 1.691 ± 0.441
0.507TyrPhe: 0.507 ± 0.253
2.791TyrGly: 2.791 ± 0.537
0.254TyrHis: 0.254 ± 0.151
0.93TyrIle: 0.93 ± 0.265
0.677TyrLys: 0.677 ± 0.192
1.945TyrLeu: 1.945 ± 0.335
0.254TyrMet: 0.254 ± 0.141
0.254TyrAsn: 0.254 ± 0.151
0.846TyrPro: 0.846 ± 0.29
1.015TyrGln: 1.015 ± 0.328
2.453TyrArg: 2.453 ± 0.445
1.184TyrSer: 1.184 ± 0.35
1.691TyrThr: 1.691 ± 0.497
1.945TyrVal: 1.945 ± 0.46
0.338TyrTrp: 0.338 ± 0.17
0.254TyrTyr: 0.254 ± 0.12
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (11825 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski