Hyaloscypha variabilis F

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Hyaloscyphaceae; Hyaloscypha; Hyaloscypha hepaticicola/Rhizoscyphus ericae species complex; Hyaloscypha variabilis

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20347 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2J6RIR4|A0A2J6RIR4_9HELO Adenylyl cyclase (Fragment) OS=Hyaloscypha variabilis F OX=1149755 GN=L207DRAFT_387266 PE=4 SV=1
MM1 pKa = 6.84VQISLLLGLAAISQVLAAPAPAPTAAPDD29 pKa = 3.58LEE31 pKa = 4.63KK32 pKa = 10.7RR33 pKa = 11.84ATTCTFSGSGGASSASKK50 pKa = 10.8SKK52 pKa = 9.59TSCSTIILSALAVPSGVTLDD72 pKa = 3.77LTDD75 pKa = 4.47LNEE78 pKa = 4.1GTTVIFEE85 pKa = 4.37GEE87 pKa = 4.14TTFGYY92 pKa = 10.01EE93 pKa = 3.88EE94 pKa = 4.03WSGPLFAVSGTDD106 pKa = 2.99ITVTQSSGAYY116 pKa = 10.26LNGNGADD123 pKa = 3.09WWDD126 pKa = 3.86GEE128 pKa = 4.29GSNGGKK134 pKa = 8.26TKK136 pKa = 10.58PKK138 pKa = 10.14FFQAHH143 pKa = 5.32NLISSTISDD152 pKa = 3.93IYY154 pKa = 10.64ILNPPVQVFSIDD166 pKa = 3.47DD167 pKa = 3.9CTDD170 pKa = 3.33LTVSGITIDD179 pKa = 3.74GTAGDD184 pKa = 4.1SLGANTDD191 pKa = 3.13GFDD194 pKa = 3.53IGDD197 pKa = 3.99STSVTISGANVYY209 pKa = 10.16NQDD212 pKa = 3.1DD213 pKa = 4.24CVAVNSGTDD222 pKa = 3.0ITFTGGVCSGGHH234 pKa = 5.7GLSIGSVGGRR244 pKa = 11.84SDD246 pKa = 3.59NTVDD250 pKa = 3.36TVNFLSSEE258 pKa = 4.31VKK260 pKa = 10.49DD261 pKa = 3.65SQNGVRR267 pKa = 11.84IKK269 pKa = 9.9TISGDD274 pKa = 3.49TGSVTGVTYY283 pKa = 11.04KK284 pKa = 10.86DD285 pKa = 3.11ITLSGITDD293 pKa = 3.35YY294 pKa = 11.65GIVVRR299 pKa = 11.84QDD301 pKa = 3.21YY302 pKa = 10.94DD303 pKa = 3.64GTSGDD308 pKa = 3.85PTNGITIKK316 pKa = 10.93DD317 pKa = 3.69FVLDD321 pKa = 3.85NVQGTVEE328 pKa = 4.11STATDD333 pKa = 3.61VYY335 pKa = 10.13ILCGSGSCTDD345 pKa = 3.52WTWTDD350 pKa = 3.32VSVTGGKK357 pKa = 10.07VSTGCLNLPSGISCDD372 pKa = 3.51VV373 pKa = 3.06

Molecular weight: 38.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2J6RZD0|A0A2J6RZD0_9HELO Uncharacterized protein OS=Hyaloscypha variabilis F OX=1149755 GN=L207DRAFT_422060 PE=4 SV=1
SS1 pKa = 6.85HH2 pKa = 7.5KK3 pKa = 10.71SFRR6 pKa = 11.84TKK8 pKa = 10.44QKK10 pKa = 10.05LARR13 pKa = 11.84AQKK16 pKa = 8.97QNRR19 pKa = 11.84PIPQWIRR26 pKa = 11.84LRR28 pKa = 11.84TGNTIRR34 pKa = 11.84YY35 pKa = 5.79NAKK38 pKa = 8.89RR39 pKa = 11.84RR40 pKa = 11.84HH41 pKa = 4.15WRR43 pKa = 11.84KK44 pKa = 7.38TRR46 pKa = 11.84IGII49 pKa = 4.0

Molecular weight: 6.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20340

7

20347

8940297

49

7742

439.4

48.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.089 ± 0.014

1.403 ± 0.008

5.344 ± 0.011

6.217 ± 0.018

4.005 ± 0.011

6.871 ± 0.015

2.308 ± 0.008

5.397 ± 0.013

4.949 ± 0.013

9.2 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.141 ± 0.006

3.816 ± 0.009

5.734 ± 0.018

3.865 ± 0.011

5.73 ± 0.016

8.308 ± 0.021

6.061 ± 0.013

6.031 ± 0.012

1.634 ± 0.007

2.896 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski