Amino acid dipepetide frequency for Gordonia phage Reyja

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.449AlaAla: 20.449 ± 3.067
1.03AlaCys: 1.03 ± 0.281
7.723AlaAsp: 7.723 ± 0.792
8.018AlaGlu: 8.018 ± 0.705
3.236AlaPhe: 3.236 ± 0.624
14.123AlaGly: 14.123 ± 1.344
3.236AlaHis: 3.236 ± 0.583
4.561AlaIle: 4.561 ± 0.629
3.972AlaLys: 3.972 ± 0.55
9.489AlaLeu: 9.489 ± 1.005
2.942AlaMet: 2.942 ± 0.531
3.384AlaAsn: 3.384 ± 0.54
5.737AlaPro: 5.737 ± 0.539
4.781AlaGln: 4.781 ± 0.688
9.636AlaArg: 9.636 ± 0.979
5.811AlaSer: 5.811 ± 0.979
7.356AlaThr: 7.356 ± 1.091
6.105AlaVal: 6.105 ± 0.641
2.133AlaTrp: 2.133 ± 0.427
2.648AlaTyr: 2.648 ± 0.444
0.0AlaXaa: 0.0 ± 0.0
Cys
0.662CysAla: 0.662 ± 0.239
0.0CysCys: 0.0 ± 0.0
0.441CysAsp: 0.441 ± 0.18
0.441CysGlu: 0.441 ± 0.156
0.074CysPhe: 0.074 ± 0.063
0.662CysGly: 0.662 ± 0.196
0.221CysHis: 0.221 ± 0.172
0.147CysIle: 0.147 ± 0.097
0.147CysLys: 0.147 ± 0.098
0.294CysLeu: 0.294 ± 0.168
0.074CysMet: 0.074 ± 0.071
0.294CysAsn: 0.294 ± 0.14
0.294CysPro: 0.294 ± 0.146
0.074CysGln: 0.074 ± 0.071
0.736CysArg: 0.736 ± 0.223
0.368CysSer: 0.368 ± 0.213
0.662CysThr: 0.662 ± 0.272
0.588CysVal: 0.588 ± 0.208
0.0CysTrp: 0.0 ± 0.0
0.294CysTyr: 0.294 ± 0.158
0.0CysXaa: 0.0 ± 0.0
Asp
7.429AspAla: 7.429 ± 0.964
0.368AspCys: 0.368 ± 0.219
5.517AspAsp: 5.517 ± 0.817
4.487AspGlu: 4.487 ± 0.66
1.618AspPhe: 1.618 ± 0.436
5.958AspGly: 5.958 ± 0.603
1.692AspHis: 1.692 ± 0.39
3.163AspIle: 3.163 ± 0.421
1.25AspLys: 1.25 ± 0.434
5.664AspLeu: 5.664 ± 0.661
0.883AspMet: 0.883 ± 0.226
2.06AspAsn: 2.06 ± 0.389
4.266AspPro: 4.266 ± 0.503
2.06AspGln: 2.06 ± 0.351
4.634AspArg: 4.634 ± 0.521
2.501AspSer: 2.501 ± 0.432
4.266AspThr: 4.266 ± 0.603
5.517AspVal: 5.517 ± 0.764
1.692AspTrp: 1.692 ± 0.308
1.692AspTyr: 1.692 ± 0.362
0.0AspXaa: 0.0 ± 0.0
Glu
6.547GluAla: 6.547 ± 0.821
0.515GluCys: 0.515 ± 0.18
2.795GluAsp: 2.795 ± 0.543
3.163GluGlu: 3.163 ± 0.429
1.398GluPhe: 1.398 ± 0.272
4.119GluGly: 4.119 ± 0.534
2.133GluHis: 2.133 ± 0.374
3.825GluIle: 3.825 ± 0.564
1.986GluLys: 1.986 ± 0.303
5.59GluLeu: 5.59 ± 0.879
1.177GluMet: 1.177 ± 0.306
2.354GluAsn: 2.354 ± 0.375
2.942GluPro: 2.942 ± 0.483
2.427GluGln: 2.427 ± 0.459
4.34GluArg: 4.34 ± 0.493
3.604GluSer: 3.604 ± 0.462
3.236GluThr: 3.236 ± 0.472
4.413GluVal: 4.413 ± 0.587
1.398GluTrp: 1.398 ± 0.328
1.398GluTyr: 1.398 ± 0.307
0.0GluXaa: 0.0 ± 0.0
Phe
2.722PheAla: 2.722 ± 0.424
0.147PheCys: 0.147 ± 0.096
1.692PheAsp: 1.692 ± 0.434
1.912PheGlu: 1.912 ± 0.443
0.515PhePhe: 0.515 ± 0.205
1.986PheGly: 1.986 ± 0.376
0.294PheHis: 0.294 ± 0.179
0.809PheIle: 0.809 ± 0.224
0.736PheLys: 0.736 ± 0.27
2.207PheLeu: 2.207 ± 0.45
0.294PheMet: 0.294 ± 0.145
0.662PheAsn: 0.662 ± 0.219
1.324PhePro: 1.324 ± 0.283
0.588PheGln: 0.588 ± 0.304
1.545PheArg: 1.545 ± 0.343
1.692PheSer: 1.692 ± 0.313
1.765PheThr: 1.765 ± 0.334
2.06PheVal: 2.06 ± 0.395
0.368PheTrp: 0.368 ± 0.156
0.736PheTyr: 0.736 ± 0.171
0.0PheXaa: 0.0 ± 0.0
Gly
8.9GlyAla: 8.9 ± 1.313
0.515GlyCys: 0.515 ± 0.187
7.356GlyAsp: 7.356 ± 0.81
4.855GlyGlu: 4.855 ± 0.579
2.942GlyPhe: 2.942 ± 0.478
8.165GlyGly: 8.165 ± 0.815
2.207GlyHis: 2.207 ± 0.516
4.487GlyIle: 4.487 ± 0.854
3.163GlyLys: 3.163 ± 0.598
6.841GlyLeu: 6.841 ± 0.651
1.398GlyMet: 1.398 ± 0.307
2.869GlyAsn: 2.869 ± 0.48
3.972GlyPro: 3.972 ± 0.556
1.765GlyGln: 1.765 ± 0.327
6.179GlyArg: 6.179 ± 0.771
4.487GlySer: 4.487 ± 0.816
6.988GlyThr: 6.988 ± 0.846
6.62GlyVal: 6.62 ± 0.642
1.618GlyTrp: 1.618 ± 0.46
3.089GlyTyr: 3.089 ± 0.346
0.0GlyXaa: 0.0 ± 0.0
His
2.869HisAla: 2.869 ± 0.553
0.147HisCys: 0.147 ± 0.103
1.177HisAsp: 1.177 ± 0.325
0.809HisGlu: 0.809 ± 0.181
0.294HisPhe: 0.294 ± 0.205
1.103HisGly: 1.103 ± 0.389
0.883HisHis: 0.883 ± 0.273
1.177HisIle: 1.177 ± 0.368
0.515HisLys: 0.515 ± 0.198
2.795HisLeu: 2.795 ± 0.65
0.515HisMet: 0.515 ± 0.205
0.809HisAsn: 0.809 ± 0.238
1.618HisPro: 1.618 ± 0.397
0.809HisGln: 0.809 ± 0.229
2.06HisArg: 2.06 ± 0.423
1.324HisSer: 1.324 ± 0.378
1.986HisThr: 1.986 ± 0.352
1.618HisVal: 1.618 ± 0.256
0.294HisTrp: 0.294 ± 0.136
0.221HisTyr: 0.221 ± 0.137
0.0HisXaa: 0.0 ± 0.0
Ile
6.547IleAla: 6.547 ± 0.502
0.147IleCys: 0.147 ± 0.096
3.825IleAsp: 3.825 ± 0.523
3.457IleGlu: 3.457 ± 0.498
0.736IlePhe: 0.736 ± 0.193
4.266IleGly: 4.266 ± 0.537
0.662IleHis: 0.662 ± 0.158
1.324IleIle: 1.324 ± 0.318
0.736IleLys: 0.736 ± 0.205
3.016IleLeu: 3.016 ± 0.436
0.588IleMet: 0.588 ± 0.2
1.765IleAsn: 1.765 ± 0.382
2.427IlePro: 2.427 ± 0.333
1.25IleGln: 1.25 ± 0.376
2.869IleArg: 2.869 ± 0.557
1.692IleSer: 1.692 ± 0.28
3.898IleThr: 3.898 ± 0.549
3.678IleVal: 3.678 ± 0.409
0.736IleTrp: 0.736 ± 0.244
0.736IleTyr: 0.736 ± 0.243
0.0IleXaa: 0.0 ± 0.0
Lys
4.708LysAla: 4.708 ± 0.877
0.294LysCys: 0.294 ± 0.131
1.03LysAsp: 1.03 ± 0.263
0.956LysGlu: 0.956 ± 0.277
0.515LysPhe: 0.515 ± 0.199
2.06LysGly: 2.06 ± 0.464
0.662LysHis: 0.662 ± 0.2
1.324LysIle: 1.324 ± 0.276
0.588LysLys: 0.588 ± 0.224
3.31LysLeu: 3.31 ± 0.485
0.515LysMet: 0.515 ± 0.163
0.441LysAsn: 0.441 ± 0.219
1.839LysPro: 1.839 ± 0.337
0.736LysGln: 0.736 ± 0.207
2.28LysArg: 2.28 ± 0.429
1.986LysSer: 1.986 ± 0.412
1.986LysThr: 1.986 ± 0.385
2.06LysVal: 2.06 ± 0.395
0.515LysTrp: 0.515 ± 0.206
0.588LysTyr: 0.588 ± 0.207
0.0LysXaa: 0.0 ± 0.0
Leu
11.328LeuAla: 11.328 ± 0.759
0.368LeuCys: 0.368 ± 0.153
5.885LeuAsp: 5.885 ± 0.555
5.075LeuGlu: 5.075 ± 0.535
1.103LeuPhe: 1.103 ± 0.243
6.547LeuGly: 6.547 ± 0.523
1.471LeuHis: 1.471 ± 0.326
3.604LeuIle: 3.604 ± 0.552
2.574LeuLys: 2.574 ± 0.606
4.561LeuLeu: 4.561 ± 0.59
1.618LeuMet: 1.618 ± 0.396
2.28LeuAsn: 2.28 ± 0.467
4.561LeuPro: 4.561 ± 0.859
1.765LeuGln: 1.765 ± 0.37
5.664LeuArg: 5.664 ± 0.698
3.604LeuSer: 3.604 ± 0.558
5.223LeuThr: 5.223 ± 0.701
6.399LeuVal: 6.399 ± 0.542
1.471LeuTrp: 1.471 ± 0.265
1.324LeuTyr: 1.324 ± 0.244
0.0LeuXaa: 0.0 ± 0.0
Met
1.398MetAla: 1.398 ± 0.325
0.147MetCys: 0.147 ± 0.1
0.956MetAsp: 0.956 ± 0.318
0.662MetGlu: 0.662 ± 0.261
0.809MetPhe: 0.809 ± 0.249
1.03MetGly: 1.03 ± 0.257
0.294MetHis: 0.294 ± 0.138
0.736MetIle: 0.736 ± 0.274
0.294MetLys: 0.294 ± 0.184
0.956MetLeu: 0.956 ± 0.213
0.294MetMet: 0.294 ± 0.152
0.662MetAsn: 0.662 ± 0.217
1.398MetPro: 1.398 ± 0.302
0.736MetGln: 0.736 ± 0.193
1.912MetArg: 1.912 ± 0.433
1.912MetSer: 1.912 ± 0.363
3.457MetThr: 3.457 ± 0.381
1.618MetVal: 1.618 ± 0.324
0.588MetTrp: 0.588 ± 0.221
0.294MetTyr: 0.294 ± 0.236
0.0MetXaa: 0.0 ± 0.0
Asn
3.898AsnAla: 3.898 ± 0.532
0.221AsnCys: 0.221 ± 0.122
2.207AsnAsp: 2.207 ± 0.403
1.912AsnGlu: 1.912 ± 0.35
0.588AsnPhe: 0.588 ± 0.266
3.604AsnGly: 3.604 ± 0.507
0.736AsnHis: 0.736 ± 0.227
1.177AsnIle: 1.177 ± 0.255
0.809AsnLys: 0.809 ± 0.286
2.28AsnLeu: 2.28 ± 0.504
0.368AsnMet: 0.368 ± 0.162
0.662AsnAsn: 0.662 ± 0.196
2.501AsnPro: 2.501 ± 0.466
0.368AsnGln: 0.368 ± 0.188
2.869AsnArg: 2.869 ± 0.574
1.545AsnSer: 1.545 ± 0.387
2.648AsnThr: 2.648 ± 0.619
1.912AsnVal: 1.912 ± 0.363
0.588AsnTrp: 0.588 ± 0.197
0.588AsnTyr: 0.588 ± 0.223
0.0AsnXaa: 0.0 ± 0.0
Pro
7.356ProAla: 7.356 ± 0.699
0.368ProCys: 0.368 ± 0.166
4.634ProAsp: 4.634 ± 0.581
4.708ProGlu: 4.708 ± 0.46
1.177ProPhe: 1.177 ± 0.274
4.928ProGly: 4.928 ± 0.527
1.25ProHis: 1.25 ± 0.301
2.648ProIle: 2.648 ± 0.416
1.471ProLys: 1.471 ± 0.327
3.457ProLeu: 3.457 ± 0.558
1.03ProMet: 1.03 ± 0.251
1.986ProAsn: 1.986 ± 0.325
4.046ProPro: 4.046 ± 0.853
2.354ProGln: 2.354 ± 0.57
3.163ProArg: 3.163 ± 0.576
3.236ProSer: 3.236 ± 0.489
5.002ProThr: 5.002 ± 0.603
4.119ProVal: 4.119 ± 0.468
1.912ProTrp: 1.912 ± 0.428
0.736ProTyr: 0.736 ± 0.198
0.0ProXaa: 0.0 ± 0.0
Gln
3.604GlnAla: 3.604 ± 0.624
0.294GlnCys: 0.294 ± 0.123
1.398GlnAsp: 1.398 ± 0.384
1.177GlnGlu: 1.177 ± 0.259
0.441GlnPhe: 0.441 ± 0.203
2.28GlnGly: 2.28 ± 0.418
0.883GlnHis: 0.883 ± 0.28
1.545GlnIle: 1.545 ± 0.261
0.662GlnLys: 0.662 ± 0.215
2.427GlnLeu: 2.427 ± 0.393
0.809GlnMet: 0.809 ± 0.22
1.03GlnAsn: 1.03 ± 0.336
1.839GlnPro: 1.839 ± 0.366
1.912GlnGln: 1.912 ± 0.435
2.795GlnArg: 2.795 ± 0.477
1.177GlnSer: 1.177 ± 0.286
1.839GlnThr: 1.839 ± 0.459
2.427GlnVal: 2.427 ± 0.451
0.662GlnTrp: 0.662 ± 0.261
0.956GlnTyr: 0.956 ± 0.261
0.0GlnXaa: 0.0 ± 0.0
Arg
8.018ArgAla: 8.018 ± 0.715
0.588ArgCys: 0.588 ± 0.215
4.928ArgAsp: 4.928 ± 0.692
4.193ArgGlu: 4.193 ± 0.829
1.839ArgPhe: 1.839 ± 0.313
5.37ArgGly: 5.37 ± 0.774
1.692ArgHis: 1.692 ± 0.502
3.457ArgIle: 3.457 ± 0.506
2.869ArgLys: 2.869 ± 0.468
5.296ArgLeu: 5.296 ± 0.736
1.912ArgMet: 1.912 ± 0.389
2.501ArgAsn: 2.501 ± 0.389
5.59ArgPro: 5.59 ± 0.848
1.765ArgGln: 1.765 ± 0.415
8.312ArgArg: 8.312 ± 1.177
3.384ArgSer: 3.384 ± 0.464
5.811ArgThr: 5.811 ± 0.55
4.928ArgVal: 4.928 ± 0.61
1.618ArgTrp: 1.618 ± 0.393
1.545ArgTyr: 1.545 ± 0.277
0.0ArgXaa: 0.0 ± 0.0
Ser
6.399SerAla: 6.399 ± 1.048
0.294SerCys: 0.294 ± 0.144
2.942SerAsp: 2.942 ± 0.386
2.722SerGlu: 2.722 ± 0.445
1.25SerPhe: 1.25 ± 0.354
5.59SerGly: 5.59 ± 0.674
0.736SerHis: 0.736 ± 0.251
2.427SerIle: 2.427 ± 0.454
1.177SerLys: 1.177 ± 0.259
3.531SerLeu: 3.531 ± 0.439
1.692SerMet: 1.692 ± 0.26
1.986SerAsn: 1.986 ± 0.398
2.942SerPro: 2.942 ± 0.478
0.956SerGln: 0.956 ± 0.209
3.457SerArg: 3.457 ± 0.573
2.354SerSer: 2.354 ± 0.426
4.487SerThr: 4.487 ± 0.75
4.119SerVal: 4.119 ± 0.464
1.03SerTrp: 1.03 ± 0.275
0.809SerTyr: 0.809 ± 0.212
0.0SerXaa: 0.0 ± 0.0
Thr
10.739ThrAla: 10.739 ± 1.217
0.441ThrCys: 0.441 ± 0.174
4.046ThrAsp: 4.046 ± 0.647
3.678ThrGlu: 3.678 ± 0.593
2.207ThrPhe: 2.207 ± 0.347
7.282ThrGly: 7.282 ± 0.997
1.177ThrHis: 1.177 ± 0.348
3.751ThrIle: 3.751 ± 0.519
1.912ThrLys: 1.912 ± 0.383
5.958ThrLeu: 5.958 ± 0.858
1.03ThrMet: 1.03 ± 0.244
1.986ThrAsn: 1.986 ± 0.372
5.443ThrPro: 5.443 ± 0.565
1.618ThrGln: 1.618 ± 0.321
5.664ThrArg: 5.664 ± 0.758
4.193ThrSer: 4.193 ± 0.519
5.664ThrThr: 5.664 ± 0.655
6.032ThrVal: 6.032 ± 0.641
0.662ThrTrp: 0.662 ± 0.235
1.545ThrTyr: 1.545 ± 0.354
0.0ThrXaa: 0.0 ± 0.0
Val
9.195ValAla: 9.195 ± 1.087
0.221ValCys: 0.221 ± 0.131
5.737ValAsp: 5.737 ± 0.755
4.928ValGlu: 4.928 ± 0.511
1.986ValPhe: 1.986 ± 0.417
5.296ValGly: 5.296 ± 0.64
1.398ValHis: 1.398 ± 0.302
3.089ValIle: 3.089 ± 0.481
2.207ValLys: 2.207 ± 0.602
4.634ValLeu: 4.634 ± 0.627
1.839ValMet: 1.839 ± 0.467
2.427ValAsn: 2.427 ± 0.5
4.487ValPro: 4.487 ± 0.557
2.795ValGln: 2.795 ± 0.439
4.487ValArg: 4.487 ± 0.701
3.531ValSer: 3.531 ± 0.495
6.252ValThr: 6.252 ± 0.669
5.737ValVal: 5.737 ± 0.541
1.103ValTrp: 1.103 ± 0.262
1.618ValTyr: 1.618 ± 0.351
0.0ValXaa: 0.0 ± 0.0
Trp
1.986TrpAla: 1.986 ± 0.271
0.221TrpCys: 0.221 ± 0.12
0.956TrpAsp: 0.956 ± 0.234
0.956TrpGlu: 0.956 ± 0.252
0.662TrpPhe: 0.662 ± 0.199
1.545TrpGly: 1.545 ± 0.384
0.809TrpHis: 0.809 ± 0.245
0.662TrpIle: 0.662 ± 0.306
0.441TrpLys: 0.441 ± 0.184
2.133TrpLeu: 2.133 ± 0.401
0.368TrpMet: 0.368 ± 0.143
0.441TrpAsn: 0.441 ± 0.149
1.25TrpPro: 1.25 ± 0.262
0.809TrpGln: 0.809 ± 0.243
1.03TrpArg: 1.03 ± 0.243
1.471TrpSer: 1.471 ± 0.308
0.809TrpThr: 0.809 ± 0.193
1.765TrpVal: 1.765 ± 0.416
0.883TrpTrp: 0.883 ± 0.31
0.074TrpTyr: 0.074 ± 0.073
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.618TyrAla: 1.618 ± 0.424
0.147TyrCys: 0.147 ± 0.103
1.471TyrAsp: 1.471 ± 0.296
1.25TyrGlu: 1.25 ± 0.327
0.662TyrPhe: 0.662 ± 0.187
2.501TyrGly: 2.501 ± 0.542
0.588TyrHis: 0.588 ± 0.235
0.441TyrIle: 0.441 ± 0.273
0.956TyrLys: 0.956 ± 0.3
1.986TyrLeu: 1.986 ± 0.284
0.736TyrMet: 0.736 ± 0.275
0.883TyrAsn: 0.883 ± 0.247
1.103TyrPro: 1.103 ± 0.286
0.588TyrGln: 0.588 ± 0.202
1.986TyrArg: 1.986 ± 0.383
1.03TyrSer: 1.03 ± 0.25
1.692TyrThr: 1.692 ± 0.326
1.25TyrVal: 1.25 ± 0.256
0.0TyrTrp: 0.0 ± 0.0
0.368TyrTyr: 0.368 ± 0.151
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 65 proteins (13596 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski