Ceratocystis fimbriata CBS 114723

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Microascales; Ceratocystidaceae; Ceratocystis; Ceratocystis fimbriata

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7264 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2C5XF84|A0A2C5XF84_9PEZI Sulfate permease 2 OS=Ceratocystis fimbriata CBS 114723 OX=1035309 GN=cys-14_1 PE=3 SV=1
MM1 pKa = 7.17KK2 pKa = 9.95TFATIVASASLVAAQGYY19 pKa = 6.88MTGCSIGNQNFKK31 pKa = 10.62FSNVSSTDD39 pKa = 2.82SYY41 pKa = 11.95SNVNADD47 pKa = 4.1RR48 pKa = 11.84ISRR51 pKa = 11.84YY52 pKa = 5.16TTGSTGVKK60 pKa = 10.14DD61 pKa = 3.79VTSLDD66 pKa = 3.9MQCGATGGKK75 pKa = 7.88GTQGAPEE82 pKa = 4.18SAKK85 pKa = 10.08VAAGEE90 pKa = 4.22SVQMHH95 pKa = 5.71WSKK98 pKa = 8.04WTKK101 pKa = 9.14VNKK104 pKa = 10.2GPTVTYY110 pKa = 9.02MAKK113 pKa = 10.44CPDD116 pKa = 4.68AGCQDD121 pKa = 4.33YY122 pKa = 11.33VPGTDD127 pKa = 3.57AVWFKK132 pKa = 10.68IQEE135 pKa = 4.41AGPTASGQWPSTSLGTSGSQDD156 pKa = 2.97TYY158 pKa = 11.03TIPEE162 pKa = 4.24CLASGNYY169 pKa = 9.23LVRR172 pKa = 11.84HH173 pKa = 5.54EE174 pKa = 5.17AISLGGSAQFYY185 pKa = 10.03IGCHH189 pKa = 4.21QLEE192 pKa = 4.51VSGSGTTNPTDD203 pKa = 3.28LVSFPGAYY211 pKa = 9.35KK212 pKa = 10.74SSDD215 pKa = 3.18SGIKK219 pKa = 9.82FDD221 pKa = 5.33PNTMPPSSYY230 pKa = 9.66TVPGPSVFTCGGSSGSTPSAAGSSSGPKK258 pKa = 9.68PSTGPSSAVSSNSPVGPSNVPMGTGNTPKK287 pKa = 9.52VTGNLPKK294 pKa = 10.37GTGNVPLGTGSVGSNGGSATTPASGYY320 pKa = 7.07QTIASVSGSASAAATTPAPGGEE342 pKa = 4.27YY343 pKa = 10.32GAEE346 pKa = 4.15DD347 pKa = 3.93VSSVPVRR354 pKa = 11.84GTGSATSASSPVTTSGPEE372 pKa = 3.84DD373 pKa = 3.83TEE375 pKa = 4.36DD376 pKa = 4.31CPVEE380 pKa = 4.39NDD382 pKa = 3.96TVPASPTSSRR392 pKa = 11.84VPLSGTNSTIPDD404 pKa = 3.91GGSTTVGSSPLTTGSTGSGSGSDD427 pKa = 3.89YY428 pKa = 11.25EE429 pKa = 5.05SGDD432 pKa = 3.73DD433 pKa = 3.67EE434 pKa = 5.88CPVEE438 pKa = 5.66DD439 pKa = 5.67GEE441 pKa = 4.57TTPAGSATTASSPASTGAGSTTPVEE466 pKa = 4.71SDD468 pKa = 4.11DD469 pKa = 4.13EE470 pKa = 4.62CPADD474 pKa = 4.43DD475 pKa = 5.41SEE477 pKa = 4.59EE478 pKa = 4.3TTSNSSPTTPEE489 pKa = 3.67TKK491 pKa = 9.99TPTGSSPSSSDD502 pKa = 2.76SKK504 pKa = 10.09TPSDD508 pKa = 4.09SSPSTSDD515 pKa = 2.71SKK517 pKa = 10.24TPTSSSLSSSGSNTPSGSSPSSYY540 pKa = 10.81GSKK543 pKa = 9.06TPSGSSPSTSDD554 pKa = 2.74SKK556 pKa = 10.21TPTSSSPSSSSSSSSTGSDD575 pKa = 3.12SYY577 pKa = 10.88STSNTEE583 pKa = 3.95STSTNTGKK591 pKa = 10.21NSYY594 pKa = 8.17STEE597 pKa = 3.85SGYY600 pKa = 11.56GSGTGSGTGTGSGSTTNPSTPKK622 pKa = 10.6SSTTSAASPQSPQTSSSASPEE643 pKa = 3.98NDD645 pKa = 3.5DD646 pKa = 3.64EE647 pKa = 5.44CPAEE651 pKa = 4.43DD652 pKa = 4.07EE653 pKa = 4.53EE654 pKa = 5.46SVEE657 pKa = 5.11EE658 pKa = 4.94DD659 pKa = 3.9DD660 pKa = 4.32EE661 pKa = 5.06CPAEE665 pKa = 4.17EE666 pKa = 5.25DD667 pKa = 3.95EE668 pKa = 4.92PATTSPVEE676 pKa = 4.15TQNDD680 pKa = 3.99DD681 pKa = 3.02QCAADD686 pKa = 5.06DD687 pKa = 4.92DD688 pKa = 4.81EE689 pKa = 4.8EE690 pKa = 4.63TTSNSSPTTPTTTEE704 pKa = 4.29EE705 pKa = 5.25DD706 pKa = 4.07DD707 pKa = 4.28DD708 pKa = 4.59ACPADD713 pKa = 4.07EE714 pKa = 5.8DD715 pKa = 4.61EE716 pKa = 4.97PTTTTPTSPSSTSGSASTSGTSTSSASPEE745 pKa = 4.13TEE747 pKa = 3.72EE748 pKa = 4.15EE749 pKa = 4.53CPVEE753 pKa = 4.03YY754 pKa = 10.49EE755 pKa = 3.94RR756 pKa = 11.84DD757 pKa = 3.33LSYY760 pKa = 11.46VEE762 pKa = 4.94EE763 pKa = 4.22NLPRR767 pKa = 11.84RR768 pKa = 11.84HH769 pKa = 5.86WDD771 pKa = 3.11RR772 pKa = 11.84RR773 pKa = 11.84SWW775 pKa = 3.2

Molecular weight: 77.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2C5WXN3|A0A2C5WXN3_9PEZI Uncharacterized protein OS=Ceratocystis fimbriata CBS 114723 OX=1035309 GN=CFIMG_007429RA00001 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.09WRR6 pKa = 11.84KK7 pKa = 8.87KK8 pKa = 8.73RR9 pKa = 11.84VRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.94RR15 pKa = 11.84KK16 pKa = 7.8RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.6MRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 11.11

Molecular weight: 3.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7264

0

7264

3783215

17

7066

520.8

57.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.052 ± 0.035

1.151 ± 0.009

5.711 ± 0.022

5.971 ± 0.033

3.431 ± 0.016

6.37 ± 0.025

2.436 ± 0.014

4.822 ± 0.02

4.871 ± 0.024

8.468 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.394 ± 0.012

3.848 ± 0.015

6.269 ± 0.033

4.09 ± 0.023

5.969 ± 0.028

9.263 ± 0.038

6.174 ± 0.025

5.943 ± 0.022

1.228 ± 0.01

2.53 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski