Trematomus bernacchii polyomavirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U9K629|A0A2U9K629_9POLY VP2 OS=Trematomus bernacchii polyomavirus 1 OX=2218588 GN=VP2 PE=4 SV=1
MM1 pKa = 7.38GLVISVVAGISAFFAEE17 pKa = 4.74LTVAAAMAGTSAEE30 pKa = 3.93AVTASFEE37 pKa = 4.22LSVVLGEE44 pKa = 4.26SAEE47 pKa = 3.99DD48 pKa = 3.49VMAGEE53 pKa = 4.72EE54 pKa = 4.06FTLFSGEE61 pKa = 3.9TAEE64 pKa = 4.37AEE66 pKa = 4.67GYY68 pKa = 10.1VDD70 pKa = 4.42EE71 pKa = 5.64GAINDD76 pKa = 3.42AWNWSPIEE84 pKa = 3.97GTTRR88 pKa = 11.84STFSGVARR96 pKa = 11.84SRR98 pKa = 11.84LAQTVGSGIVAASVIGAVAGTQLEE122 pKa = 4.38GDD124 pKa = 3.43QGLDD128 pKa = 2.94MVRR131 pKa = 11.84AVFNPEE137 pKa = 3.34SSGDD141 pKa = 3.67HH142 pKa = 5.31TVSDD146 pKa = 3.48TMEE149 pKa = 5.78RR150 pKa = 11.84IMTPEE155 pKa = 3.84EE156 pKa = 4.61YY157 pKa = 9.61IDD159 pKa = 3.55NMQILSEE166 pKa = 4.29EE167 pKa = 4.45YY168 pKa = 10.6SNGMLSRR175 pKa = 11.84DD176 pKa = 3.71LPLSVAVADD185 pKa = 3.72YY186 pKa = 10.56SAAIGRR192 pKa = 11.84SGRR195 pKa = 11.84LAHH198 pKa = 6.55RR199 pKa = 11.84TRR201 pKa = 11.84EE202 pKa = 3.95TMGADD207 pKa = 3.59KK208 pKa = 11.02SDD210 pKa = 3.91LLIFSAMKK218 pKa = 10.4SEE220 pKa = 4.22PKK222 pKa = 10.17VGSWTLPGADD232 pKa = 4.91GIPITRR238 pKa = 11.84AAWNRR243 pKa = 11.84YY244 pKa = 5.2TMGYY248 pKa = 8.53YY249 pKa = 10.2HH250 pKa = 6.81GTAAQMRR257 pKa = 11.84EE258 pKa = 3.98MLAHH262 pKa = 6.66GEE264 pKa = 4.25VLRR267 pKa = 11.84QAGQNIWPLFQRR279 pKa = 11.84AAHH282 pKa = 6.64LAAAPVAVALDD293 pKa = 3.67AALDD297 pKa = 3.92VPQDD301 pKa = 3.44GVRR304 pKa = 11.84QQLRR308 pKa = 11.84GIGRR312 pKa = 11.84RR313 pKa = 11.84AAHH316 pKa = 6.85RR317 pKa = 11.84LAGDD321 pKa = 3.41AGVAVAQLGDD331 pKa = 3.51RR332 pKa = 11.84VANALVAGGNRR343 pKa = 11.84AGHH346 pKa = 5.24QAVEE350 pKa = 4.25TVIAALTYY358 pKa = 10.58GVTEE362 pKa = 4.34YY363 pKa = 10.77FSSTSSPKK371 pKa = 9.92PGPDD375 pKa = 3.4AIEE378 pKa = 5.23IEE380 pKa = 4.95DD381 pKa = 5.12DD382 pKa = 3.63PDD384 pKa = 3.57LPKK387 pKa = 10.59EE388 pKa = 3.98IEE390 pKa = 4.09VDD392 pKa = 2.65GRR394 pKa = 11.84TYY396 pKa = 11.08VKK398 pKa = 10.54EE399 pKa = 3.87KK400 pKa = 11.0ADD402 pKa = 3.78LHH404 pKa = 5.94TVTHH408 pKa = 6.82PEE410 pKa = 3.49GTYY413 pKa = 8.94THH415 pKa = 7.07TEE417 pKa = 4.01VHH419 pKa = 5.99WYY421 pKa = 9.69EE422 pKa = 3.92PTEE425 pKa = 4.27RR426 pKa = 11.84PQIAPGYY433 pKa = 7.93DD434 pKa = 2.93TAFKK438 pKa = 10.12YY439 pKa = 10.74AEE441 pKa = 4.43PRR443 pKa = 11.84SPDD446 pKa = 3.06QMLALTEE453 pKa = 3.89VLGYY457 pKa = 9.59FVHH460 pKa = 7.21GYY462 pKa = 10.64DD463 pKa = 3.19ITPPLTTSQRR473 pKa = 11.84LILSALTLQVARR485 pKa = 11.84RR486 pKa = 11.84HH487 pKa = 5.42ASRR490 pKa = 11.84KK491 pKa = 8.13RR492 pKa = 11.84RR493 pKa = 11.84IRR495 pKa = 11.84GTEE498 pKa = 3.3HH499 pKa = 7.24RR500 pKa = 11.84SRR502 pKa = 11.84SKK504 pKa = 10.67RR505 pKa = 11.84KK506 pKa = 9.72RR507 pKa = 11.84EE508 pKa = 3.82AA509 pKa = 3.11

Molecular weight: 54.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U9K628|A0A2U9K628_9POLY Large T antigen OS=Trematomus bernacchii polyomavirus 1 OX=2218588 PE=4 SV=1
MM1 pKa = 7.43PVVKK5 pKa = 10.34GGYY8 pKa = 8.35EE9 pKa = 3.87VLNIVADD16 pKa = 4.19LNEE19 pKa = 4.23SEE21 pKa = 5.34RR22 pKa = 11.84LDD24 pKa = 3.62INCYY28 pKa = 9.42LAPEE32 pKa = 5.46AYY34 pKa = 7.92TQAVGSNKK42 pKa = 9.61SPKK45 pKa = 8.99WLTSAIDD52 pKa = 3.69LPKK55 pKa = 10.38IPVAAKK61 pKa = 10.09GNKK64 pKa = 8.29IKK66 pKa = 10.1IWEE69 pKa = 4.48CYY71 pKa = 9.04KK72 pKa = 10.85CSTGVLTGDD81 pKa = 3.61PNHH84 pKa = 6.14VNEE87 pKa = 5.18IPSQSPYY94 pKa = 10.43NGPSRR99 pKa = 11.84SSWCVSGLPMEE110 pKa = 5.83GFGKK114 pKa = 9.73WPFPEE119 pKa = 5.62APPQPSILMKK129 pKa = 10.59NDD131 pKa = 4.16LYY133 pKa = 11.33DD134 pKa = 3.81STDD137 pKa = 3.78FTPNPWMEE145 pKa = 3.66NAKK148 pKa = 10.64YY149 pKa = 10.14YY150 pKa = 10.47VSKK153 pKa = 11.1QMGTQSYY160 pKa = 10.32AAGDD164 pKa = 3.89NNSSVVLLADD174 pKa = 3.77ADD176 pKa = 4.3GFGIMCPQGSCFISALDD193 pKa = 4.22FFTQYY198 pKa = 10.9ISGKK202 pKa = 7.47TVFQCYY208 pKa = 9.53QIPRR212 pKa = 11.84YY213 pKa = 8.96FKK215 pKa = 10.55LYY217 pKa = 10.09FRR219 pKa = 11.84QRR221 pKa = 11.84WVKK224 pKa = 10.61SPVVLMDD231 pKa = 5.24LFTQMVNQQVQGYY244 pKa = 9.54AGEE247 pKa = 4.27TGNIEE252 pKa = 4.3HH253 pKa = 7.11VSLAWGKK260 pKa = 10.5RR261 pKa = 11.84DD262 pKa = 3.72LVPGGLLGNTLNKK275 pKa = 10.3LGAGTSTSTTMDD287 pKa = 3.33TEE289 pKa = 4.39EE290 pKa = 4.62PPALPVKK297 pKa = 10.03LAKK300 pKa = 10.49SQVGSYY306 pKa = 9.11KK307 pKa = 10.35QPSASKK313 pKa = 9.69IPPGVTITNKK323 pKa = 10.31

Molecular weight: 35.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1504

323

672

501.3

55.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.71 ± 1.925

2.128 ± 0.932

5.718 ± 0.556

6.316 ± 0.696

3.856 ± 0.737

7.779 ± 0.576

2.194 ± 0.462

4.322 ± 0.163

5.319 ± 1.258

7.713 ± 0.25

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.593 ± 0.112

3.457 ± 0.763

5.718 ± 0.79

3.989 ± 0.32

5.053 ± 1.034

7.513 ± 0.34

5.851 ± 0.63

7.181 ± 0.204

1.263 ± 0.28

3.324 ± 0.402
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski