Gordonia phage DumpsterDude

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ruthyvirus; Gordonia virus DumpsterDude

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8R065|A0A6G8R065_9CAUD Tail terminator OS=Gordonia phage DumpsterDude OX=2713262 GN=11 PE=4 SV=1
MM1 pKa = 7.29TAYY4 pKa = 10.34QLVAIEE10 pKa = 4.2FTEE13 pKa = 4.12VLARR17 pKa = 11.84NEE19 pKa = 4.04KK20 pKa = 9.46GQPTKK25 pKa = 9.87IHH27 pKa = 7.64RR28 pKa = 11.84YY29 pKa = 7.93RR30 pKa = 11.84QGDD33 pKa = 3.89TIEE36 pKa = 4.19LTGSEE41 pKa = 4.21EE42 pKa = 3.88QRR44 pKa = 11.84LVKK47 pKa = 10.59AGAVVPVVEE56 pKa = 4.72VEE58 pKa = 4.15PVDD61 pKa = 5.23AGDD64 pKa = 4.43PPADD68 pKa = 4.44GPDD71 pKa = 3.51GDD73 pKa = 5.57DD74 pKa = 4.03PDD76 pKa = 5.18GSGDD80 pKa = 4.56GDD82 pKa = 5.18DD83 pKa = 5.46SDD85 pKa = 5.36PDD87 pKa = 4.01PDD89 pKa = 5.68GDD91 pKa = 5.33DD92 pKa = 3.35EE93 pKa = 4.97LKK95 pKa = 10.54RR96 pKa = 11.84PRR98 pKa = 11.84KK99 pKa = 9.78AGPVDD104 pKa = 3.25DD105 pKa = 3.69WRR107 pKa = 11.84AYY109 pKa = 10.1AVSKK113 pKa = 11.24GIDD116 pKa = 3.29ADD118 pKa = 3.69KK119 pKa = 11.49AEE121 pKa = 4.2GMTKK125 pKa = 10.5AEE127 pKa = 4.75LIEE130 pKa = 4.16LVGGEE135 pKa = 4.26DD136 pKa = 3.27

Molecular weight: 14.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8R0C2|A0A6G8R0C2_9CAUD BrnT-like toxin OS=Gordonia phage DumpsterDude OX=2713262 GN=38 PE=4 SV=1
MM1 pKa = 7.62AHH3 pKa = 6.69RR4 pKa = 11.84RR5 pKa = 11.84WPKK8 pKa = 9.51GSWMRR13 pKa = 11.84LEE15 pKa = 4.18SAATLRR21 pKa = 11.84AIMGQRR27 pKa = 11.84KK28 pKa = 7.87MSMARR33 pKa = 11.84LARR36 pKa = 11.84YY37 pKa = 8.81AGCSKK42 pKa = 10.95SFISALCLEE51 pKa = 5.03RR52 pKa = 11.84KK53 pKa = 5.99TTCTPQLAEE62 pKa = 4.94RR63 pKa = 11.84IAEE66 pKa = 4.08ALEE69 pKa = 4.36VPLSLLFVVNEE80 pKa = 4.22SAASGRR86 pKa = 11.84NTQKK90 pKa = 10.58VSAA93 pKa = 4.53

Molecular weight: 10.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

16402

47

1652

231.0

24.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.486 ± 0.375

0.902 ± 0.162

6.987 ± 0.328

5.548 ± 0.32

2.652 ± 0.161

8.725 ± 0.514

2.128 ± 0.224

4.688 ± 0.218

3.445 ± 0.342

7.731 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.799 ± 0.114

2.701 ± 0.159

5.786 ± 0.217

3.176 ± 0.153

7.389 ± 0.464

5.505 ± 0.216

6.871 ± 0.286

7.603 ± 0.269

1.89 ± 0.119

1.988 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski