Streptomyces phage Chymera

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142K666|A0A142K666_9CAUD Uncharacterized protein OS=Streptomyces phage Chymera OX=1821728 GN=SEA_CHYMERA_40 PE=4 SV=1
MM1 pKa = 7.28ATDD4 pKa = 3.94PTDD7 pKa = 3.95FCPNEE12 pKa = 3.99QCGGARR18 pKa = 11.84DD19 pKa = 3.93ACGCPSDD26 pKa = 4.93LTTQSLMVCLVCGGRR41 pKa = 11.84WWRR44 pKa = 11.84PYY46 pKa = 10.95DD47 pKa = 3.72SDD49 pKa = 4.93SFRR52 pKa = 11.84CPHH55 pKa = 6.9PGCGQDD61 pKa = 3.34MSLITIPAARR71 pKa = 11.84RR72 pKa = 11.84PEE74 pKa = 4.14RR75 pKa = 11.84GEE77 pKa = 4.31DD78 pKa = 3.75GQPLDD83 pKa = 4.47TDD85 pKa = 4.49DD86 pKa = 5.91GPDD89 pKa = 3.46GDD91 pKa = 3.91EE92 pKa = 4.28CHH94 pKa = 7.05AEE96 pKa = 4.45FIDD99 pKa = 6.05GSWTYY104 pKa = 10.27CGCPEE109 pKa = 4.15CDD111 pKa = 3.01EE112 pKa = 5.31RR113 pKa = 11.84EE114 pKa = 4.22EE115 pKa = 4.65NDD117 pKa = 3.73TEE119 pKa = 4.17MEE121 pKa = 4.33LYY123 pKa = 10.54GG124 pKa = 4.47

Molecular weight: 13.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142K681|A0A142K681_9CAUD HNH endonuclease OS=Streptomyces phage Chymera OX=1821728 GN=SEA_CHYMERA_55 PE=4 SV=1
MM1 pKa = 7.95PSRR4 pKa = 11.84APRR7 pKa = 11.84RR8 pKa = 11.84CTTPGCGAMAAPPSAKK24 pKa = 9.81CAPHH28 pKa = 7.05DD29 pKa = 3.41KK30 pKa = 10.3RR31 pKa = 11.84RR32 pKa = 11.84PRR34 pKa = 11.84RR35 pKa = 11.84SASAKK40 pKa = 9.84GYY42 pKa = 9.32NAEE45 pKa = 3.83HH46 pKa = 5.7VQRR49 pKa = 11.84FRR51 pKa = 11.84AGVLEE56 pKa = 4.23RR57 pKa = 11.84DD58 pKa = 3.98ATCVLCKK65 pKa = 10.07AAPATQADD73 pKa = 4.03HH74 pKa = 6.73WPLSKK79 pKa = 10.69RR80 pKa = 11.84EE81 pKa = 3.94LQARR85 pKa = 11.84GLDD88 pKa = 3.35EE89 pKa = 4.89HH90 pKa = 7.12DD91 pKa = 3.59PRR93 pKa = 11.84RR94 pKa = 11.84GRR96 pKa = 11.84GLCASCHH103 pKa = 5.38SKK105 pKa = 8.52EE106 pKa = 4.47TARR109 pKa = 11.84HH110 pKa = 5.5QPGGWNAGGPRR121 pKa = 11.84YY122 pKa = 9.99

Molecular weight: 13.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

10702

36

922

194.6

21.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.091 ± 0.564

1.084 ± 0.223

6.382 ± 0.241

5.896 ± 0.358

2.009 ± 0.191

8.839 ± 0.326

2.495 ± 0.217

3.457 ± 0.29

2.439 ± 0.367

9.064 ± 0.324

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.065 ± 0.186

1.859 ± 0.146

6.513 ± 0.447

3.42 ± 0.206

8.802 ± 0.453

4.793 ± 0.264

6.569 ± 0.363

7.073 ± 0.336

1.878 ± 0.12

2.271 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski