Paenibacillus pasadenensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4980 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N5NB45|A0A2N5NB45_9BACL 3'-phosphate/5'-hydroxy nucleic acid ligase OS=Paenibacillus pasadenensis OX=217090 GN=B8V81_1800 PE=3 SV=1
MM1 pKa = 7.54PPSLIKK7 pKa = 10.61HH8 pKa = 5.87FLVVTSTVSGTVDD21 pKa = 3.34TTTVTAVDD29 pKa = 3.72DD30 pKa = 4.02AVTRR34 pKa = 11.84FSATVTSAMIDD45 pKa = 3.25AGAGTVTIPAEE56 pKa = 4.26SFFDD60 pKa = 3.9DD61 pKa = 4.92AGDD64 pKa = 3.91AVPTGSLPVVSAGGYY79 pKa = 8.46VNVYY83 pKa = 9.19VNGMLQQGALTTTSPTTVVIASADD107 pKa = 3.64IVPGTPVILEE117 pKa = 3.65FHH119 pKa = 6.98SYY121 pKa = 11.25DD122 pKa = 3.37GTTSTSTSVPSLIVNSTITSS142 pKa = 3.46

Molecular weight: 14.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N5NC56|A0A2N5NC56_9BACL Uncharacterized protein OS=Paenibacillus pasadenensis OX=217090 GN=B8V81_0036 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.82PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 9.99RR21 pKa = 11.84MSSKK25 pKa = 10.47NGRR28 pKa = 11.84KK29 pKa = 9.01VLAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.58GRR39 pKa = 11.84AKK41 pKa = 10.7LSAA44 pKa = 3.92

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4980

0

4980

1626338

37

4173

326.6

35.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.231 ± 0.066

0.698 ± 0.01

5.109 ± 0.025

6.902 ± 0.041

3.614 ± 0.025

8.694 ± 0.046

1.816 ± 0.016

5.029 ± 0.035

3.981 ± 0.037

10.772 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.457 ± 0.015

2.584 ± 0.03

4.853 ± 0.04

3.471 ± 0.02

6.804 ± 0.051

6.277 ± 0.03

4.705 ± 0.035

6.67 ± 0.041

1.33 ± 0.014

3.003 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski