Brazilian marseillevirus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Marseilleviridae; Marseillevirus; unclassified Marseillevirus

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 491 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142CKN4|A0A142CKN4_9VIRU DUF5858 domain-containing protein OS=Brazilian marseillevirus OX=1813599 PE=4 SV=1
MM1 pKa = 7.38QNTTNFFRR9 pKa = 11.84DD10 pKa = 3.14ISLVGSVTSLPPVLSRR26 pKa = 11.84GTLVYY31 pKa = 10.64PEE33 pKa = 5.22DD34 pKa = 3.8SSNLYY39 pKa = 9.91ISNGSQWVIAGGDD52 pKa = 3.71VGPLAAQVAQNTADD66 pKa = 3.3IGVLQGDD73 pKa = 3.92VSTLQGEE80 pKa = 4.8VATNTTDD87 pKa = 3.38IAGLQTDD94 pKa = 3.94VSTLQGQVSSLSTDD108 pKa = 3.05VSALQTQVATNTTDD122 pKa = 2.61IGTLQGDD129 pKa = 3.76VSTLQGEE136 pKa = 4.85VATNTADD143 pKa = 3.34ISTLQGDD150 pKa = 4.07VSTLQTQVNANTLDD164 pKa = 3.27ISALQTQVATNTTDD178 pKa = 2.61IGTLQGDD185 pKa = 3.76VSTLQGEE192 pKa = 4.8VATNTSNITTLQGQVATNTLDD213 pKa = 3.05IGTLQTDD220 pKa = 3.6VTTLQGDD227 pKa = 3.79VSTLQTQVGTNTSNITTLQGQVATNTTDD255 pKa = 2.86ISALQTQVSTNTTNIGTLQGDD276 pKa = 3.84VSTLQTQVTTNTLDD290 pKa = 2.96IGNLQTDD297 pKa = 4.08VSTLQTDD304 pKa = 4.24VAQNTLDD311 pKa = 3.23ISGLQGQVATNTTNIATNTSNISTLQGQTATNTTNITTLQGQVSTNTSDD360 pKa = 3.16ISTLQTQVSTNTADD374 pKa = 2.92IGTLQGQVATNTTNIATNTSNISALQGQVSTNTSNISTLQTTKK417 pKa = 10.58EE418 pKa = 4.04DD419 pKa = 3.52KK420 pKa = 10.68SNKK423 pKa = 8.73GVSGGYY429 pKa = 10.08CPLTVSATPVVPNTNLAVATKK450 pKa = 8.93TVNGSLYY457 pKa = 9.96FPFYY461 pKa = 10.63TRR463 pKa = 11.84LSLTADD469 pKa = 4.01WSPGTATGTINRR481 pKa = 11.84WVVQNDD487 pKa = 3.09TSYY490 pKa = 11.99ALNNAYY496 pKa = 10.04GAGPKK501 pKa = 10.08GATNITFWKK510 pKa = 9.15CPRR513 pKa = 11.84DD514 pKa = 3.56AVVLFVFNIAFSVATSTNRR533 pKa = 11.84FFSIVITPVVPANTPPPYY551 pKa = 10.09QYY553 pKa = 11.6NFGLDD558 pKa = 3.77NYY560 pKa = 10.25ASSYY564 pKa = 11.27SFASSLIIPLRR575 pKa = 11.84QGDD578 pKa = 3.72QLQINWSMAGQSPAFQTTYY597 pKa = 10.23TYY599 pKa = 9.78WHH601 pKa = 6.7INEE604 pKa = 4.39MSFGVYY610 pKa = 10.13DD611 pKa = 3.98PNLL614 pKa = 3.53

Molecular weight: 64.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142CJG9|A0A142CJG9_9VIRU MORN repeat-containing protein OS=Brazilian marseillevirus OX=1813599 PE=4 SV=1
MM1 pKa = 6.85QARR4 pKa = 11.84IRR6 pKa = 11.84EE7 pKa = 4.12YY8 pKa = 10.32QRR10 pKa = 11.84LFPSVKK16 pKa = 9.53GLKK19 pKa = 9.29IVPSDD24 pKa = 3.74RR25 pKa = 11.84SQKK28 pKa = 9.79RR29 pKa = 11.84YY30 pKa = 9.81KK31 pKa = 9.93ATFVMDD37 pKa = 3.85GEE39 pKa = 4.5EE40 pKa = 4.19KK41 pKa = 10.51VVHH44 pKa = 6.29FGQRR48 pKa = 11.84GAFTFADD55 pKa = 4.51GAPEE59 pKa = 4.34SKK61 pKa = 10.11RR62 pKa = 11.84QAYY65 pKa = 8.43RR66 pKa = 11.84ARR68 pKa = 11.84HH69 pKa = 4.98SKK71 pKa = 9.98ILNKK75 pKa = 10.06GRR77 pKa = 11.84TAYY80 pKa = 9.31KK81 pKa = 10.41VPGSASSLAWVILWW95 pKa = 3.75

Molecular weight: 10.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

491

0

491

116783

34

1553

237.8

27.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.175 ± 0.119

2.435 ± 0.102

4.935 ± 0.066

8.161 ± 0.14

5.736 ± 0.087

6.122 ± 0.113

1.848 ± 0.064

5.004 ± 0.072

8.541 ± 0.18

8.905 ± 0.111

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.16 ± 0.053

4.203 ± 0.081

3.847 ± 0.087

3.364 ± 0.082

5.435 ± 0.093

7.615 ± 0.103

5.24 ± 0.118

6.673 ± 0.074

1.463 ± 0.048

3.139 ± 0.061
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski