Blastomyces percursus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Onygenales;

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10283 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J9R7U1|A0A1J9R7U1_9EURO tRNA pseudouridine(38-40) synthase OS=Blastomyces percursus OX=1658174 GN=ACJ73_04574 PE=3 SV=1
MM1 pKa = 7.79DD2 pKa = 3.82MAAIEE7 pKa = 4.27MMDD10 pKa = 2.87IGYY13 pKa = 10.17DD14 pKa = 3.23EE15 pKa = 5.59GGDD18 pKa = 3.63YY19 pKa = 10.9SYY21 pKa = 11.52DD22 pKa = 3.43EE23 pKa = 5.37PYY25 pKa = 10.93NDD27 pKa = 5.19SEE29 pKa = 4.78SCDD32 pKa = 3.6TEE34 pKa = 5.61DD35 pKa = 3.94YY36 pKa = 10.56TGSSYY41 pKa = 11.31NRR43 pKa = 11.84NNSPSYY49 pKa = 11.11DD50 pKa = 3.6YY51 pKa = 10.19GTDD54 pKa = 3.52GASEE58 pKa = 4.49GNGPRR63 pKa = 11.84DD64 pKa = 3.38AEE66 pKa = 4.08EE67 pKa = 4.18YY68 pKa = 9.43PRR70 pKa = 11.84KK71 pKa = 9.58EE72 pKa = 4.11LEE74 pKa = 3.97VDD76 pKa = 3.22SDD78 pKa = 3.67EE79 pKa = 4.36ATYY82 pKa = 11.17SSGSSCDD89 pKa = 3.34GSLQAEE95 pKa = 4.26AEE97 pKa = 4.07SSYY100 pKa = 11.18RR101 pKa = 11.84HH102 pKa = 5.97ASVPSGSPYY111 pKa = 10.55RR112 pKa = 11.84STRR115 pKa = 11.84WGGG118 pKa = 3.05

Molecular weight: 12.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J9Q1C8|A0A1J9Q1C8_9EURO Uncharacterized protein OS=Blastomyces percursus OX=1658174 GN=ACJ73_09958 PE=4 SV=1
MM1 pKa = 7.14VFQWLQRR8 pKa = 11.84AWFTRR13 pKa = 11.84QGITYY18 pKa = 9.23FGLPIHH24 pKa = 6.77PMLGNGGNTSSTRR37 pKa = 11.84LIQFSISASTGRR49 pKa = 11.84YY50 pKa = 6.8VVCSTTHH57 pKa = 6.89PSSQTAPLMLANQRR71 pKa = 11.84IAWEE75 pKa = 4.03MSEE78 pKa = 3.93SGEE81 pKa = 4.01IFVRR85 pKa = 11.84SCADD89 pKa = 3.27VKK91 pKa = 10.81RR92 pKa = 11.84PPHH95 pKa = 6.51LLINNQLVIKK105 pKa = 10.32VKK107 pKa = 10.71LHH109 pKa = 6.06DD110 pKa = 3.83TFEE113 pKa = 4.19NAAFKK118 pKa = 11.12LNFSICTTRR127 pKa = 11.84RR128 pKa = 11.84YY129 pKa = 9.7HH130 pKa = 6.92DD131 pKa = 3.98CFRR134 pKa = 11.84AIWLRR139 pKa = 11.84APDD142 pKa = 4.05APAATTPQPVSEE154 pKa = 4.35TNSNIRR160 pKa = 11.84SRR162 pKa = 11.84LRR164 pKa = 11.84LFVRR168 pKa = 11.84QSHH171 pKa = 5.52GVNPKK176 pKa = 9.42PPIRR180 pKa = 11.84SRR182 pKa = 11.84SNAGCCLEE190 pKa = 3.96RR191 pKa = 11.84SGTGVVNIDD200 pKa = 3.45VGSNPTNRR208 pKa = 11.84RR209 pKa = 11.84GKK211 pKa = 9.25VLSGSTCASPFPVGVDD227 pKa = 3.29PDD229 pKa = 3.75LANAPEE235 pKa = 4.48HH236 pKa = 6.3PRR238 pKa = 11.84SAASRR243 pKa = 11.84EE244 pKa = 4.14QSSGTTLMRR253 pKa = 11.84PPQILASSDD262 pKa = 3.21APRR265 pKa = 11.84DD266 pKa = 3.61VPATFFIFSDD276 pKa = 3.65LCVRR280 pKa = 11.84TAGWYY285 pKa = 9.49RR286 pKa = 11.84LRR288 pKa = 11.84FQLVDD293 pKa = 3.4VQNRR297 pKa = 11.84GSPHH301 pKa = 6.52AFTRR305 pKa = 11.84SGLNLSKK312 pKa = 10.88CSLRR316 pKa = 11.84KK317 pKa = 9.66IFLGG321 pKa = 3.78

Molecular weight: 35.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10283

0

10283

4463529

30

5875

434.1

48.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.997 ± 0.019

1.229 ± 0.01

5.666 ± 0.016

6.296 ± 0.024

3.577 ± 0.015

6.594 ± 0.021

2.508 ± 0.009

5.043 ± 0.015

5.041 ± 0.022

8.813 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.078 ± 0.008

3.914 ± 0.013

6.311 ± 0.025

4.107 ± 0.019

6.576 ± 0.018

8.635 ± 0.027

5.901 ± 0.017

5.739 ± 0.015

1.304 ± 0.008

2.672 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski