Paenibacillus phage Gryphonian

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fernvirus; unclassified Fernvirus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345AYW7|A0A345AYW7_9CAUD Resolvase OS=Paenibacillus phage Gryphonian OX=2249775 GN=LYCANUS2_60 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.05IKK4 pKa = 10.36YY5 pKa = 8.81RR6 pKa = 11.84LSIGYY11 pKa = 8.57PGAVRR16 pKa = 11.84EE17 pKa = 4.52DD18 pKa = 3.58EE19 pKa = 4.69VEE21 pKa = 3.93IDD23 pKa = 3.95DD24 pKa = 5.19EE25 pKa = 4.32EE26 pKa = 6.18LEE28 pKa = 4.26GLTPEE33 pKa = 4.09EE34 pKa = 4.6AEE36 pKa = 5.63DD37 pKa = 4.53RR38 pKa = 11.84ICDD41 pKa = 3.35IVNEE45 pKa = 4.18HH46 pKa = 5.74VQDD49 pKa = 5.35FISLSWEE56 pKa = 4.02EE57 pKa = 3.99VEE59 pKa = 4.75EE60 pKa = 4.21

Molecular weight: 7.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345AYU2|A0A345AYU2_9CAUD Putative membrane lipoprotein OS=Paenibacillus phage Gryphonian OX=2249775 GN=LYCANUS2_33 PE=4 SV=1
MM1 pKa = 7.84WYY3 pKa = 10.57NGTSNSGLYY12 pKa = 10.09VGGRR16 pKa = 11.84RR17 pKa = 11.84HH18 pKa = 5.74PLNRR22 pKa = 11.84KK23 pKa = 8.66GVTPMEE29 pKa = 4.24TFQALQLMFLFGMFILALLTFIKK52 pKa = 10.47KK53 pKa = 9.92

Molecular weight: 6.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

12085

36

878

183.1

20.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.645 ± 0.422

0.902 ± 0.123

5.627 ± 0.286

8.109 ± 0.424

3.815 ± 0.21

6.206 ± 0.372

1.903 ± 0.186

7.174 ± 0.339

8.804 ± 0.355

8.457 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.962 ± 0.177

4.51 ± 0.187

3.335 ± 0.203

4.187 ± 0.236

4.576 ± 0.298

6.074 ± 0.264

5.453 ± 0.281

6.165 ± 0.224

1.498 ± 0.164

3.6 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski