Tuhoko virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Rubulavirinae; Pararubulavirus; Tuhoko pararubulavirus 1

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D8WJ23|D8WJ23_9MONO Phosphoprotein OS=Tuhoko virus 1 OX=798072 GN=V/P PE=3 SV=1
MM1 pKa = 7.37SSVFKK6 pKa = 10.59AYY8 pKa = 10.12EE9 pKa = 3.97LFNLEE14 pKa = 4.0QEE16 pKa = 4.28QQEE19 pKa = 4.35RR20 pKa = 11.84GNDD23 pKa = 3.31LVLPPEE29 pKa = 4.21TLRR32 pKa = 11.84SNIRR36 pKa = 11.84VFVLNTQDD44 pKa = 3.22PEE46 pKa = 3.68IRR48 pKa = 11.84FRR50 pKa = 11.84MMCFCIRR57 pKa = 11.84LVASNSARR65 pKa = 11.84SAHH68 pKa = 5.69RR69 pKa = 11.84TGALLTLLSLPTAAMQNHH87 pKa = 5.79IRR89 pKa = 11.84IADD92 pKa = 4.23RR93 pKa = 11.84SPDD96 pKa = 3.44AEE98 pKa = 3.89IEE100 pKa = 4.18RR101 pKa = 11.84IEE103 pKa = 4.55IEE105 pKa = 4.11GFEE108 pKa = 3.96QGSYY112 pKa = 11.2KK113 pKa = 10.72LIPNARR119 pKa = 11.84TPMSPAEE126 pKa = 4.05ITALDD131 pKa = 4.05AMATDD136 pKa = 4.74LPDD139 pKa = 5.41SIMYY143 pKa = 7.57DD144 pKa = 3.54TPFMNMYY151 pKa = 9.44TEE153 pKa = 5.72AEE155 pKa = 4.22GCDD158 pKa = 3.7EE159 pKa = 4.16IEE161 pKa = 4.31EE162 pKa = 4.1FLEE165 pKa = 4.12AVYY168 pKa = 10.84SVLIQVWITVCKK180 pKa = 10.58CMTAYY185 pKa = 9.75DD186 pKa = 3.94QPTGSDD192 pKa = 3.55EE193 pKa = 4.19KK194 pKa = 11.32RR195 pKa = 11.84MAKK198 pKa = 9.77YY199 pKa = 9.33QQQGRR204 pKa = 11.84LDD206 pKa = 3.58PKK208 pKa = 9.71YY209 pKa = 9.02TLQQEE214 pKa = 4.37VRR216 pKa = 11.84RR217 pKa = 11.84LIQKK221 pKa = 8.65CIRR224 pKa = 11.84GSLPVRR230 pKa = 11.84QFLAFEE236 pKa = 4.33LQTARR241 pKa = 11.84KK242 pKa = 9.05QGTITSKK249 pKa = 9.51YY250 pKa = 8.2YY251 pKa = 11.52SMVGDD256 pKa = 3.56ISKK259 pKa = 10.72YY260 pKa = 9.72IEE262 pKa = 4.15NAGMSAFFMTARR274 pKa = 11.84YY275 pKa = 9.81ALGTRR280 pKa = 11.84WAPLALAAFTGDD292 pKa = 3.72LLKK295 pKa = 10.49MKK297 pKa = 10.44SLMLLYY303 pKa = 10.46RR304 pKa = 11.84KK305 pKa = 9.91LGEE308 pKa = 4.08KK309 pKa = 10.29ARR311 pKa = 11.84FMALLEE317 pKa = 4.48MPDD320 pKa = 3.33MMEE323 pKa = 4.7FAPANYY329 pKa = 9.99NLLYY333 pKa = 10.18SYY335 pKa = 11.64AMGIGSVMDD344 pKa = 3.55AQMRR348 pKa = 11.84NYY350 pKa = 10.61NFARR354 pKa = 11.84PFLNTAYY361 pKa = 10.01FQLGVEE367 pKa = 4.51TANKK371 pKa = 8.68QQGAVDD377 pKa = 3.57KK378 pKa = 11.61NMAAEE383 pKa = 4.73LGLSEE388 pKa = 4.75DD389 pKa = 4.66DD390 pKa = 3.88KK391 pKa = 11.62RR392 pKa = 11.84VMAQTVSRR400 pKa = 11.84LTGNKK405 pKa = 9.73AGSDD409 pKa = 3.77NQDD412 pKa = 3.25LMDD415 pKa = 3.83MLSRR419 pKa = 11.84RR420 pKa = 11.84SRR422 pKa = 11.84SGQTRR427 pKa = 11.84ALRR430 pKa = 11.84MSAQGNQAVDD440 pKa = 3.33GDD442 pKa = 4.18EE443 pKa = 4.41EE444 pKa = 5.09DD445 pKa = 4.91DD446 pKa = 3.89EE447 pKa = 4.83EE448 pKa = 4.73EE449 pKa = 4.31EE450 pKa = 4.32EE451 pKa = 4.19EE452 pKa = 4.49EE453 pKa = 4.15EE454 pKa = 5.12ARR456 pKa = 11.84DD457 pKa = 3.69LTEE460 pKa = 4.34EE461 pKa = 4.02EE462 pKa = 4.39QKK464 pKa = 11.04EE465 pKa = 4.21SEE467 pKa = 3.89AWLKK471 pKa = 9.53KK472 pKa = 8.6WKK474 pKa = 10.14EE475 pKa = 3.78IEE477 pKa = 4.23AQRR480 pKa = 11.84HH481 pKa = 3.87AAIEE485 pKa = 3.84AMKK488 pKa = 9.88KK489 pKa = 9.75HH490 pKa = 5.71KK491 pKa = 10.1AARR494 pKa = 11.84TQPSQMPSEE503 pKa = 4.24VQVHH507 pKa = 5.82NEE509 pKa = 3.94NQDD512 pKa = 3.05VRR514 pKa = 11.84GDD516 pKa = 3.61LDD518 pKa = 3.5SS519 pKa = 4.11

Molecular weight: 59.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D8WJ26|D8WJ26_9MONO Fusion glycoprotein F0 OS=Tuhoko virus 1 OX=798072 GN=F PE=3 SV=1
MM1 pKa = 7.74ASLIGASIPIPVNPDD16 pKa = 2.94SGKK19 pKa = 11.18SNLKK23 pKa = 9.36PFPIIKK29 pKa = 10.37APADD33 pKa = 3.65QSGHH37 pKa = 4.52TGKK40 pKa = 9.91IVRR43 pKa = 11.84QIRR46 pKa = 11.84IKK48 pKa = 10.91NLTPKK53 pKa = 10.38GSTEE57 pKa = 3.81IPITFMNTYY66 pKa = 10.74GFIKK70 pKa = 9.72PIWTHH75 pKa = 6.11GEE77 pKa = 3.97FFSEE81 pKa = 4.15FHH83 pKa = 6.95RR84 pKa = 11.84NNSTPSLTACMLPFGTGPFVDD105 pKa = 4.48NPSKK109 pKa = 11.07LLGDD113 pKa = 3.97LEE115 pKa = 4.71KK116 pKa = 10.74IQINVKK122 pKa = 10.0KK123 pKa = 10.24SASLRR128 pKa = 11.84EE129 pKa = 3.91EE130 pKa = 4.07VIFDD134 pKa = 3.38IKK136 pKa = 10.06TIPFAFTKK144 pKa = 9.86FQIGRR149 pKa = 11.84EE150 pKa = 3.91RR151 pKa = 11.84IICVSSDD158 pKa = 3.39RR159 pKa = 11.84YY160 pKa = 9.84VKK162 pKa = 10.85CPGKK166 pKa = 8.98LTSGVEE172 pKa = 3.81YY173 pKa = 9.79SYY175 pKa = 11.22CIAFISLTFCPEE187 pKa = 3.77SYY189 pKa = 10.27KK190 pKa = 10.63FRR192 pKa = 11.84VARR195 pKa = 11.84PLQQLRR201 pKa = 11.84TSYY204 pKa = 9.68MRR206 pKa = 11.84AIQLEE211 pKa = 4.56IIMKK215 pKa = 9.66IDD217 pKa = 3.93CAPDD221 pKa = 3.51SPIKK225 pKa = 10.65RR226 pKa = 11.84NLIYY230 pKa = 10.79DD231 pKa = 4.02KK232 pKa = 11.41EE233 pKa = 4.08NDD235 pKa = 3.17IYY237 pKa = 10.71IASVWLHH244 pKa = 6.24LCNILKK250 pKa = 10.35GKK252 pKa = 8.84NHH254 pKa = 6.73FKK256 pKa = 10.46TYY258 pKa = 10.64DD259 pKa = 3.2DD260 pKa = 3.96TYY262 pKa = 11.17FANKK266 pKa = 8.08CRR268 pKa = 11.84KK269 pKa = 7.54MQLVCGIADD278 pKa = 3.27MWGPTIIVHH287 pKa = 5.66SKK289 pKa = 10.27GRR291 pKa = 11.84IPKK294 pKa = 9.75SAAPYY299 pKa = 9.8FNSKK303 pKa = 8.39GWACHH308 pKa = 5.82PLSEE312 pKa = 4.46IAPSITKK319 pKa = 9.99ILWSVGATILQVNAVLQPSDD339 pKa = 3.18ISSAAGTSDD348 pKa = 5.18LIYY351 pKa = 10.09PKK353 pKa = 10.91VKK355 pKa = 10.31INPDD359 pKa = 3.2LAEE362 pKa = 3.92EE363 pKa = 5.32RR364 pKa = 11.84GIKK367 pKa = 8.86WNPLRR372 pKa = 11.84KK373 pKa = 9.16AVHH376 pKa = 5.25STT378 pKa = 3.02

Molecular weight: 42.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

1

7

4944

252

2274

706.3

79.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.655 ± 0.885

1.922 ± 0.289

5.239 ± 0.3

5.057 ± 0.637

3.236 ± 0.26

4.511 ± 0.306

2.124 ± 0.333

8.212 ± 0.737

5.218 ± 0.523

10.558 ± 1.375

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.447 ± 0.502

5.178 ± 0.416

5.502 ± 0.829

4.956 ± 0.447

4.49 ± 0.386

8.657 ± 0.403

6.29 ± 0.384

5.259 ± 0.315

1.032 ± 0.185

3.459 ± 0.483
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski