Malassezia restricta CBS 7877

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina; Malasseziomycetes; Malasseziales; Malasseziaceae; Malassezia; Malassezia restricta

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4096 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2S953|A0A3G2S953_9BASI Uncharacterized protein OS=Malassezia restricta CBS 7877 OX=425264 GN=DNF11_2819 PE=4 SV=1
MM1 pKa = 7.68APLSFYY7 pKa = 11.32DD8 pKa = 3.88EE9 pKa = 4.63VEE11 pKa = 4.3LEE13 pKa = 4.01DD14 pKa = 4.22MEE16 pKa = 4.71YY17 pKa = 11.1DD18 pKa = 3.42EE19 pKa = 6.16DD20 pKa = 3.94KK21 pKa = 11.32QLFHH25 pKa = 6.61YY26 pKa = 7.81PCPCGDD32 pKa = 3.38RR33 pKa = 11.84FEE35 pKa = 5.22ISIAQLRR42 pKa = 11.84AEE44 pKa = 4.5EE45 pKa = 4.33DD46 pKa = 3.57VARR49 pKa = 11.84CPSCTLLIRR58 pKa = 11.84VIYY61 pKa = 10.37DD62 pKa = 3.38PLDD65 pKa = 3.85FMDD68 pKa = 5.17SDD70 pKa = 4.32GANPPAVSPMVSAAAA85 pKa = 3.8

Molecular weight: 9.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2SBL7|A0A3G2SBL7_9BASI Sporulation protein RMD8 OS=Malassezia restricta CBS 7877 OX=425264 GN=RMD8 PE=3 SV=1
MM1 pKa = 6.56VQTVASDD8 pKa = 3.37AQLAFPWRR16 pKa = 11.84EE17 pKa = 3.8CVQGFFLRR25 pKa = 11.84YY26 pKa = 8.31PNPQASHH33 pKa = 5.76VLSVDD38 pKa = 3.22VVARR42 pKa = 11.84RR43 pKa = 11.84IEE45 pKa = 4.07EE46 pKa = 4.02RR47 pKa = 11.84APPEE51 pKa = 4.43PGPLPRR57 pKa = 11.84LVLCTSRR64 pKa = 11.84LILKK68 pKa = 10.16SGSLPSWVPRR78 pKa = 11.84NIIQQTTSWVLEE90 pKa = 4.18DD91 pKa = 4.71SEE93 pKa = 4.76VDD95 pKa = 4.04LSAEE99 pKa = 4.16APRR102 pKa = 11.84VMSIWTRR109 pKa = 11.84NLDD112 pKa = 3.68HH113 pKa = 6.92TSVLAVTEE121 pKa = 4.06ALTLRR126 pKa = 11.84EE127 pKa = 4.68DD128 pKa = 3.97GASTRR133 pKa = 11.84MHH135 pKa = 6.72IDD137 pKa = 2.91ADD139 pKa = 3.79VRR141 pKa = 11.84SGVSFRR147 pKa = 11.84LLRR150 pKa = 11.84RR151 pKa = 11.84RR152 pKa = 11.84IEE154 pKa = 4.09KK155 pKa = 10.26FGHH158 pKa = 5.55KK159 pKa = 9.71RR160 pKa = 11.84YY161 pKa = 9.27LSHH164 pKa = 6.64MEE166 pKa = 3.95SSRR169 pKa = 11.84HH170 pKa = 4.19GLIWAILRR178 pKa = 11.84IAPHH182 pKa = 6.53LVHH185 pKa = 6.87TPQPQAPVPYY195 pKa = 9.72RR196 pKa = 11.84QRR198 pKa = 11.84LLHH201 pKa = 6.41ALRR204 pKa = 11.84PPFLDD209 pKa = 5.37GYY211 pKa = 10.07PLTPWQWLKK220 pKa = 10.77KK221 pKa = 9.05QGRR224 pKa = 11.84RR225 pKa = 11.84LRR227 pKa = 11.84DD228 pKa = 3.29KK229 pKa = 10.65FRR231 pKa = 11.84QRR233 pKa = 11.84PTDD236 pKa = 3.27TQTT239 pKa = 2.9

Molecular weight: 27.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4096

0

4096

2061471

69

4842

503.3

55.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.846 ± 0.05

1.286 ± 0.014

5.911 ± 0.02

6.022 ± 0.03

3.249 ± 0.023

5.68 ± 0.031

3.275 ± 0.021

3.983 ± 0.026

3.774 ± 0.036

9.741 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.757 ± 0.014

2.72 ± 0.023

6.254 ± 0.044

4.26 ± 0.022

6.967 ± 0.032

7.791 ± 0.044

5.677 ± 0.02

6.701 ± 0.026

1.43 ± 0.015

2.673 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski