Amino acid dipepetide frequency for Vibrio phage Phriendly

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.93AlaAla: 6.93 ± 0.877
0.907AlaCys: 0.907 ± 0.198
4.21AlaAsp: 4.21 ± 0.475
5.44AlaGlu: 5.44 ± 0.553
2.461AlaPhe: 2.461 ± 0.405
5.505AlaGly: 5.505 ± 0.875
1.813AlaHis: 1.813 ± 0.318
4.857AlaIle: 4.857 ± 0.573
5.311AlaLys: 5.311 ± 0.677
7.707AlaLeu: 7.707 ± 0.848
2.655AlaMet: 2.655 ± 0.504
4.015AlaAsn: 4.015 ± 0.534
2.85AlaPro: 2.85 ± 0.405
3.432AlaGln: 3.432 ± 0.489
4.015AlaArg: 4.015 ± 0.465
4.274AlaSer: 4.274 ± 0.603
4.274AlaThr: 4.274 ± 0.572
5.375AlaVal: 5.375 ± 0.586
0.842AlaTrp: 0.842 ± 0.231
2.72AlaTyr: 2.72 ± 0.389
0.0AlaXaa: 0.0 ± 0.0
Cys
1.036CysAla: 1.036 ± 0.296
0.194CysCys: 0.194 ± 0.109
0.777CysAsp: 0.777 ± 0.284
0.583CysGlu: 0.583 ± 0.175
0.583CysPhe: 0.583 ± 0.215
1.101CysGly: 1.101 ± 0.337
0.518CysHis: 0.518 ± 0.245
0.648CysIle: 0.648 ± 0.203
0.971CysLys: 0.971 ± 0.293
0.648CysLeu: 0.648 ± 0.206
0.324CysMet: 0.324 ± 0.173
0.648CysAsn: 0.648 ± 0.188
0.324CysPro: 0.324 ± 0.133
0.453CysGln: 0.453 ± 0.22
0.971CysArg: 0.971 ± 0.303
0.453CysSer: 0.453 ± 0.185
0.453CysThr: 0.453 ± 0.141
0.712CysVal: 0.712 ± 0.178
0.13CysTrp: 0.13 ± 0.078
0.259CysTyr: 0.259 ± 0.133
0.0CysXaa: 0.0 ± 0.0
Asp
4.922AspAla: 4.922 ± 0.646
0.648AspCys: 0.648 ± 0.241
3.886AspAsp: 3.886 ± 0.535
4.987AspGlu: 4.987 ± 0.638
2.591AspPhe: 2.591 ± 0.555
5.829AspGly: 5.829 ± 0.692
1.166AspHis: 1.166 ± 0.268
5.051AspIle: 5.051 ± 0.63
4.598AspLys: 4.598 ± 0.606
5.375AspLeu: 5.375 ± 0.68
1.813AspMet: 1.813 ± 0.35
3.627AspAsn: 3.627 ± 0.442
2.591AspPro: 2.591 ± 0.425
1.49AspGln: 1.49 ± 0.279
1.684AspArg: 1.684 ± 0.337
3.951AspSer: 3.951 ± 0.509
3.562AspThr: 3.562 ± 0.486
3.886AspVal: 3.886 ± 0.502
1.425AspTrp: 1.425 ± 0.325
2.979AspTyr: 2.979 ± 0.498
0.0AspXaa: 0.0 ± 0.0
Glu
6.994GluAla: 6.994 ± 0.958
0.389GluCys: 0.389 ± 0.165
4.728GluAsp: 4.728 ± 0.641
6.347GluGlu: 6.347 ± 1.351
2.008GluPhe: 2.008 ± 0.341
4.598GluGly: 4.598 ± 0.485
1.49GluHis: 1.49 ± 0.327
3.238GluIle: 3.238 ± 0.441
4.987GluLys: 4.987 ± 0.765
7.448GluLeu: 7.448 ± 0.869
2.461GluMet: 2.461 ± 0.353
2.85GluAsn: 2.85 ± 0.47
1.684GluPro: 1.684 ± 0.351
3.562GluGln: 3.562 ± 0.485
3.109GluArg: 3.109 ± 0.486
3.173GluSer: 3.173 ± 0.549
3.044GluThr: 3.044 ± 0.422
5.44GluVal: 5.44 ± 0.69
1.166GluTrp: 1.166 ± 0.291
2.979GluTyr: 2.979 ± 0.397
0.0GluXaa: 0.0 ± 0.0
Phe
2.396PheAla: 2.396 ± 0.304
0.389PheCys: 0.389 ± 0.169
2.85PheAsp: 2.85 ± 0.419
2.526PheGlu: 2.526 ± 0.503
1.295PhePhe: 1.295 ± 0.289
2.137PheGly: 2.137 ± 0.309
0.648PheHis: 0.648 ± 0.222
2.202PheIle: 2.202 ± 0.385
2.785PheLys: 2.785 ± 0.453
2.72PheLeu: 2.72 ± 0.38
1.23PheMet: 1.23 ± 0.396
3.109PheAsn: 3.109 ± 0.47
1.295PhePro: 1.295 ± 0.288
0.971PheGln: 0.971 ± 0.222
1.166PheArg: 1.166 ± 0.267
2.655PheSer: 2.655 ± 0.434
1.684PheThr: 1.684 ± 0.354
2.85PheVal: 2.85 ± 0.392
0.583PheTrp: 0.583 ± 0.202
1.36PheTyr: 1.36 ± 0.302
0.0PheXaa: 0.0 ± 0.0
Gly
5.57GlyAla: 5.57 ± 0.732
1.101GlyCys: 1.101 ± 0.322
4.857GlyAsp: 4.857 ± 0.584
4.792GlyGlu: 4.792 ± 0.561
3.238GlyPhe: 3.238 ± 0.478
6.671GlyGly: 6.671 ± 1.063
1.36GlyHis: 1.36 ± 0.275
5.051GlyIle: 5.051 ± 0.57
4.922GlyLys: 4.922 ± 0.494
5.311GlyLeu: 5.311 ± 0.737
2.137GlyMet: 2.137 ± 0.319
5.246GlyAsn: 5.246 ± 0.621
0.259GlyPro: 0.259 ± 0.158
2.526GlyGln: 2.526 ± 0.455
3.691GlyArg: 3.691 ± 0.555
5.051GlySer: 5.051 ± 0.645
5.181GlyThr: 5.181 ± 0.844
4.533GlyVal: 4.533 ± 0.499
0.712GlyTrp: 0.712 ± 0.202
2.655GlyTyr: 2.655 ± 0.513
0.0GlyXaa: 0.0 ± 0.0
His
1.23HisAla: 1.23 ± 0.303
0.389HisCys: 0.389 ± 0.161
1.036HisAsp: 1.036 ± 0.247
0.842HisGlu: 0.842 ± 0.213
0.907HisPhe: 0.907 ± 0.307
1.619HisGly: 1.619 ± 0.372
0.648HisHis: 0.648 ± 0.188
1.749HisIle: 1.749 ± 0.35
1.554HisLys: 1.554 ± 0.365
1.684HisLeu: 1.684 ± 0.301
0.712HisMet: 0.712 ± 0.199
1.36HisAsn: 1.36 ± 0.297
0.971HisPro: 0.971 ± 0.3
0.971HisGln: 0.971 ± 0.29
0.648HisArg: 0.648 ± 0.236
0.777HisSer: 0.777 ± 0.245
0.971HisThr: 0.971 ± 0.217
1.425HisVal: 1.425 ± 0.364
0.389HisTrp: 0.389 ± 0.136
1.23HisTyr: 1.23 ± 0.271
0.0HisXaa: 0.0 ± 0.0
Ile
3.432IleAla: 3.432 ± 0.406
0.907IleCys: 0.907 ± 0.233
4.21IleAsp: 4.21 ± 0.521
3.044IleGlu: 3.044 ± 0.423
1.878IlePhe: 1.878 ± 0.274
3.109IleGly: 3.109 ± 0.452
1.036IleHis: 1.036 ± 0.234
2.396IleIle: 2.396 ± 0.438
4.792IleLys: 4.792 ± 0.649
4.469IleLeu: 4.469 ± 0.615
1.49IleMet: 1.49 ± 0.325
3.821IleAsn: 3.821 ± 0.375
2.202IlePro: 2.202 ± 0.374
1.813IleGln: 1.813 ± 0.359
3.044IleArg: 3.044 ± 0.412
3.173IleSer: 3.173 ± 0.516
4.404IleThr: 4.404 ± 0.619
3.756IleVal: 3.756 ± 0.484
0.842IleTrp: 0.842 ± 0.239
1.878IleTyr: 1.878 ± 0.386
0.0IleXaa: 0.0 ± 0.0
Lys
6.152LysAla: 6.152 ± 0.726
0.583LysCys: 0.583 ± 0.22
5.505LysAsp: 5.505 ± 0.545
5.829LysGlu: 5.829 ± 0.659
2.331LysPhe: 2.331 ± 0.389
4.598LysGly: 4.598 ± 0.491
1.878LysHis: 1.878 ± 0.318
3.756LysIle: 3.756 ± 0.525
4.339LysLys: 4.339 ± 0.715
5.311LysLeu: 5.311 ± 0.648
2.526LysMet: 2.526 ± 0.415
2.785LysAsn: 2.785 ± 0.444
2.526LysPro: 2.526 ± 0.334
4.08LysGln: 4.08 ± 0.565
3.173LysArg: 3.173 ± 0.56
2.914LysSer: 2.914 ± 0.444
3.562LysThr: 3.562 ± 0.486
4.857LysVal: 4.857 ± 0.548
0.842LysTrp: 0.842 ± 0.221
2.72LysTyr: 2.72 ± 0.404
0.0LysXaa: 0.0 ± 0.0
Leu
5.958LeuAla: 5.958 ± 0.758
0.907LeuCys: 0.907 ± 0.273
6.023LeuAsp: 6.023 ± 0.495
6.865LeuGlu: 6.865 ± 0.657
2.914LeuPhe: 2.914 ± 0.525
6.476LeuGly: 6.476 ± 0.794
1.036LeuHis: 1.036 ± 0.216
3.756LeuIle: 3.756 ± 0.494
5.116LeuLys: 5.116 ± 0.657
5.246LeuLeu: 5.246 ± 0.526
2.461LeuMet: 2.461 ± 0.464
3.951LeuAsn: 3.951 ± 0.476
2.979LeuPro: 2.979 ± 0.42
3.432LeuGln: 3.432 ± 0.478
3.562LeuArg: 3.562 ± 0.582
4.145LeuSer: 4.145 ± 0.589
4.145LeuThr: 4.145 ± 0.491
4.469LeuVal: 4.469 ± 0.538
0.712LeuTrp: 0.712 ± 0.25
2.591LeuTyr: 2.591 ± 0.531
0.0LeuXaa: 0.0 ± 0.0
Met
2.655MetAla: 2.655 ± 0.459
0.583MetCys: 0.583 ± 0.209
1.878MetAsp: 1.878 ± 0.289
1.943MetGlu: 1.943 ± 0.355
1.425MetPhe: 1.425 ± 0.33
2.137MetGly: 2.137 ± 0.494
0.777MetHis: 0.777 ± 0.185
1.101MetIle: 1.101 ± 0.275
2.008MetLys: 2.008 ± 0.391
2.267MetLeu: 2.267 ± 0.405
0.971MetMet: 0.971 ± 0.235
1.554MetAsn: 1.554 ± 0.315
1.749MetPro: 1.749 ± 0.231
1.36MetGln: 1.36 ± 0.328
1.49MetArg: 1.49 ± 0.313
2.072MetSer: 2.072 ± 0.379
2.072MetThr: 2.072 ± 0.417
0.971MetVal: 0.971 ± 0.227
0.324MetTrp: 0.324 ± 0.14
1.101MetTyr: 1.101 ± 0.262
0.0MetXaa: 0.0 ± 0.0
Asn
4.08AsnAla: 4.08 ± 0.613
0.583AsnCys: 0.583 ± 0.216
2.914AsnAsp: 2.914 ± 0.393
3.886AsnGlu: 3.886 ± 0.701
2.267AsnPhe: 2.267 ± 0.495
5.181AsnGly: 5.181 ± 0.693
0.971AsnHis: 0.971 ± 0.271
3.303AsnIle: 3.303 ± 0.548
3.951AsnLys: 3.951 ± 0.5
4.015AsnLeu: 4.015 ± 0.526
1.166AsnMet: 1.166 ± 0.3
2.785AsnAsn: 2.785 ± 0.453
2.785AsnPro: 2.785 ± 0.385
0.971AsnGln: 0.971 ± 0.209
3.109AsnArg: 3.109 ± 0.363
3.109AsnSer: 3.109 ± 0.423
3.886AsnThr: 3.886 ± 0.473
3.044AsnVal: 3.044 ± 0.497
0.971AsnTrp: 0.971 ± 0.258
2.655AsnTyr: 2.655 ± 0.382
0.0AsnXaa: 0.0 ± 0.0
Pro
2.785ProAla: 2.785 ± 0.403
0.259ProCys: 0.259 ± 0.122
1.943ProAsp: 1.943 ± 0.379
3.432ProGlu: 3.432 ± 0.42
1.619ProPhe: 1.619 ± 0.306
0.453ProGly: 0.453 ± 0.189
0.842ProHis: 0.842 ± 0.307
1.619ProIle: 1.619 ± 0.349
2.914ProLys: 2.914 ± 0.56
2.331ProLeu: 2.331 ± 0.378
1.23ProMet: 1.23 ± 0.263
2.461ProAsn: 2.461 ± 0.39
1.166ProPro: 1.166 ± 0.413
1.554ProGln: 1.554 ± 0.321
1.425ProArg: 1.425 ± 0.276
2.591ProSer: 2.591 ± 0.595
2.137ProThr: 2.137 ± 0.454
2.785ProVal: 2.785 ± 0.375
0.842ProTrp: 0.842 ± 0.192
1.101ProTyr: 1.101 ± 0.254
0.0ProXaa: 0.0 ± 0.0
Gln
3.562GlnAla: 3.562 ± 0.539
0.389GlnCys: 0.389 ± 0.174
2.461GlnAsp: 2.461 ± 0.398
2.979GlnGlu: 2.979 ± 0.624
1.101GlnPhe: 1.101 ± 0.252
2.591GlnGly: 2.591 ± 0.477
0.971GlnHis: 0.971 ± 0.232
2.396GlnIle: 2.396 ± 0.406
2.137GlnLys: 2.137 ± 0.358
3.691GlnLeu: 3.691 ± 0.571
0.971GlnMet: 0.971 ± 0.228
1.943GlnAsn: 1.943 ± 0.37
1.295GlnPro: 1.295 ± 0.264
2.202GlnGln: 2.202 ± 0.407
2.331GlnArg: 2.331 ± 0.388
2.331GlnSer: 2.331 ± 0.321
1.554GlnThr: 1.554 ± 0.309
2.526GlnVal: 2.526 ± 0.468
0.907GlnTrp: 0.907 ± 0.192
1.49GlnTyr: 1.49 ± 0.248
0.0GlnXaa: 0.0 ± 0.0
Arg
3.497ArgAla: 3.497 ± 0.514
0.777ArgCys: 0.777 ± 0.234
3.497ArgAsp: 3.497 ± 0.564
3.109ArgGlu: 3.109 ± 0.503
1.554ArgPhe: 1.554 ± 0.277
2.785ArgGly: 2.785 ± 0.436
1.295ArgHis: 1.295 ± 0.272
2.137ArgIle: 2.137 ± 0.344
3.562ArgLys: 3.562 ± 0.563
3.497ArgLeu: 3.497 ± 0.459
1.684ArgMet: 1.684 ± 0.368
2.85ArgAsn: 2.85 ± 0.355
1.295ArgPro: 1.295 ± 0.267
1.878ArgGln: 1.878 ± 0.333
1.813ArgArg: 1.813 ± 0.377
2.072ArgSer: 2.072 ± 0.303
2.396ArgThr: 2.396 ± 0.444
2.85ArgVal: 2.85 ± 0.4
0.907ArgTrp: 0.907 ± 0.202
1.813ArgTyr: 1.813 ± 0.416
0.0ArgXaa: 0.0 ± 0.0
Ser
4.145SerAla: 4.145 ± 0.57
0.712SerCys: 0.712 ± 0.275
2.785SerAsp: 2.785 ± 0.386
3.886SerGlu: 3.886 ± 0.65
2.526SerPhe: 2.526 ± 0.35
5.44SerGly: 5.44 ± 0.793
0.971SerHis: 0.971 ± 0.233
3.432SerIle: 3.432 ± 0.518
2.914SerLys: 2.914 ± 0.448
3.109SerLeu: 3.109 ± 0.434
1.619SerMet: 1.619 ± 0.337
2.526SerAsn: 2.526 ± 0.457
1.878SerPro: 1.878 ± 0.371
2.137SerGln: 2.137 ± 0.328
3.238SerArg: 3.238 ± 0.497
3.238SerSer: 3.238 ± 0.531
3.821SerThr: 3.821 ± 0.506
3.238SerVal: 3.238 ± 0.487
1.23SerTrp: 1.23 ± 0.301
1.943SerTyr: 1.943 ± 0.335
0.0SerXaa: 0.0 ± 0.0
Thr
4.857ThrAla: 4.857 ± 0.586
0.453ThrCys: 0.453 ± 0.186
3.821ThrAsp: 3.821 ± 0.545
3.238ThrGlu: 3.238 ± 0.476
2.202ThrPhe: 2.202 ± 0.327
6.023ThrGly: 6.023 ± 0.569
1.036ThrHis: 1.036 ± 0.294
3.044ThrIle: 3.044 ± 0.402
4.339ThrLys: 4.339 ± 0.523
4.274ThrLeu: 4.274 ± 0.643
1.295ThrMet: 1.295 ± 0.274
3.109ThrAsn: 3.109 ± 0.397
2.85ThrPro: 2.85 ± 0.465
2.008ThrGln: 2.008 ± 0.334
2.072ThrArg: 2.072 ± 0.335
2.202ThrSer: 2.202 ± 0.377
2.979ThrThr: 2.979 ± 0.513
3.821ThrVal: 3.821 ± 0.46
0.712ThrTrp: 0.712 ± 0.273
1.554ThrTyr: 1.554 ± 0.382
0.0ThrXaa: 0.0 ± 0.0
Val
6.217ValAla: 6.217 ± 0.808
0.648ValCys: 0.648 ± 0.249
4.857ValAsp: 4.857 ± 0.638
4.598ValGlu: 4.598 ± 0.593
2.461ValPhe: 2.461 ± 0.481
4.987ValGly: 4.987 ± 0.954
1.49ValHis: 1.49 ± 0.242
3.173ValIle: 3.173 ± 0.511
4.987ValLys: 4.987 ± 0.634
3.756ValLeu: 3.756 ± 0.472
2.072ValMet: 2.072 ± 0.37
3.303ValAsn: 3.303 ± 0.495
2.785ValPro: 2.785 ± 0.431
2.202ValGln: 2.202 ± 0.419
2.655ValArg: 2.655 ± 0.414
3.627ValSer: 3.627 ± 0.484
2.979ValThr: 2.979 ± 0.453
4.404ValVal: 4.404 ± 0.463
1.295ValTrp: 1.295 ± 0.313
2.461ValTyr: 2.461 ± 0.352
0.0ValXaa: 0.0 ± 0.0
Trp
0.842TrpAla: 0.842 ± 0.214
0.389TrpCys: 0.389 ± 0.177
1.23TrpAsp: 1.23 ± 0.302
0.907TrpGlu: 0.907 ± 0.284
0.453TrpPhe: 0.453 ± 0.17
1.036TrpGly: 1.036 ± 0.229
0.389TrpHis: 0.389 ± 0.165
0.971TrpIle: 0.971 ± 0.263
1.101TrpLys: 1.101 ± 0.244
1.166TrpLeu: 1.166 ± 0.256
0.389TrpMet: 0.389 ± 0.14
1.036TrpAsn: 1.036 ± 0.203
0.194TrpPro: 0.194 ± 0.116
0.971TrpGln: 0.971 ± 0.291
0.648TrpArg: 0.648 ± 0.225
0.971TrpSer: 0.971 ± 0.237
0.842TrpThr: 0.842 ± 0.201
1.295TrpVal: 1.295 ± 0.298
0.324TrpTrp: 0.324 ± 0.163
0.583TrpTyr: 0.583 ± 0.205
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.331TyrAla: 2.331 ± 0.468
0.583TyrCys: 0.583 ± 0.213
2.591TyrAsp: 2.591 ± 0.425
2.461TyrGlu: 2.461 ± 0.339
1.036TyrPhe: 1.036 ± 0.256
2.591TyrGly: 2.591 ± 0.419
0.777TyrHis: 0.777 ± 0.255
1.813TyrIle: 1.813 ± 0.391
2.979TyrLys: 2.979 ± 0.432
2.785TyrLeu: 2.785 ± 0.348
1.166TyrMet: 1.166 ± 0.312
2.655TyrAsn: 2.655 ± 0.445
1.813TyrPro: 1.813 ± 0.334
1.813TyrGln: 1.813 ± 0.336
1.425TyrArg: 1.425 ± 0.336
2.008TyrSer: 2.008 ± 0.332
1.943TyrThr: 1.943 ± 0.331
2.72TyrVal: 2.72 ± 0.458
0.583TyrTrp: 0.583 ± 0.262
1.23TyrTyr: 1.23 ± 0.343
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 71 proteins (15442 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski