Halopiger xanaduensis (strain DSM 18323 / JCM 14033 / SH-6)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales; Natrialbaceae; Halopiger; Halopiger xanaduensis

Average proteome isoelectric point is 4.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4221 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8DE38|F8DE38_HALXS Uncharacterized protein OS=Halopiger xanaduensis (strain DSM 18323 / JCM 14033 / SH-6) OX=797210 GN=Halxa_0064 PE=4 SV=1
MM1 pKa = 6.76QQTIEE6 pKa = 4.2TKK8 pKa = 9.72EE9 pKa = 4.04VNGQTVEE16 pKa = 3.76ITRR19 pKa = 11.84VDD21 pKa = 4.03GEE23 pKa = 4.68VKK25 pKa = 9.68TATIRR30 pKa = 11.84SDD32 pKa = 2.98EE33 pKa = 4.12SDD35 pKa = 3.69RR36 pKa = 11.84YY37 pKa = 8.67TSAASSDD44 pKa = 3.28VGTQFVGTEE53 pKa = 4.17SLWDD57 pKa = 3.38NMDD60 pKa = 3.86YY61 pKa = 11.63NKK63 pKa = 9.86MSQILSWVSDD73 pKa = 2.91AWNSHH78 pKa = 6.04IEE80 pKa = 4.08DD81 pKa = 4.71DD82 pKa = 4.57GDD84 pKa = 3.6YY85 pKa = 11.11DD86 pKa = 4.2SWFTAQGPAGVTIPEE101 pKa = 4.42SEE103 pKa = 4.57LSNSSDD109 pKa = 3.57LGDD112 pKa = 3.99RR113 pKa = 11.84LSDD116 pKa = 3.47ADD118 pKa = 3.26EE119 pKa = 3.97WLRR122 pKa = 11.84DD123 pKa = 3.87NEE125 pKa = 4.07GDD127 pKa = 3.93FYY129 pKa = 11.3DD130 pKa = 4.88GPSSIIVVDD139 pKa = 3.54YY140 pKa = 11.35HH141 pKa = 8.63GDD143 pKa = 3.47DD144 pKa = 4.22DD145 pKa = 3.86NTYY148 pKa = 10.33GIGYY152 pKa = 9.01VGRR155 pKa = 11.84AGEE158 pKa = 4.1SSYY161 pKa = 11.79KK162 pKa = 9.89NALVDD167 pKa = 3.94VHH169 pKa = 8.37HH170 pKa = 7.2EE171 pKa = 4.41DD172 pKa = 3.97NNNLPSEE179 pKa = 4.06LSEE182 pKa = 4.15VEE184 pKa = 4.15SEE186 pKa = 4.01GVAFHH191 pKa = 6.91EE192 pKa = 4.81LLHH195 pKa = 8.27IYY197 pKa = 10.24DD198 pKa = 4.08ATHH201 pKa = 6.91PSDD204 pKa = 3.18VSTRR208 pKa = 11.84SGNPDD213 pKa = 4.17EE214 pKa = 4.38ISIMYY219 pKa = 9.77SWDD222 pKa = 3.36GVSCSDD228 pKa = 5.03NGDD231 pKa = 3.48TEE233 pKa = 5.29QIVEE237 pKa = 4.27WVSDD241 pKa = 3.71CTEE244 pKa = 4.4GSVHH248 pKa = 7.4SYY250 pKa = 10.34IDD252 pKa = 3.53SNFF255 pKa = 3.01

Molecular weight: 28.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8D5X0|F8D5X0_HALXS Glutamine--fructose-6-phosphate aminotransferase [isomerizing] OS=Halopiger xanaduensis (strain DSM 18323 / JCM 14033 / SH-6) OX=797210 GN=glmS PE=3 SV=1
MM1 pKa = 7.46RR2 pKa = 11.84VVRR5 pKa = 11.84VNRR8 pKa = 11.84SKK10 pKa = 10.81RR11 pKa = 11.84ANHH14 pKa = 6.68FGTICEE20 pKa = 3.75KK21 pKa = 10.64RR22 pKa = 11.84MARR25 pKa = 11.84KK26 pKa = 9.31RR27 pKa = 11.84RR28 pKa = 11.84FNLEE32 pKa = 3.4RR33 pKa = 11.84ASWHH37 pKa = 6.34DD38 pKa = 3.27ARR40 pKa = 11.84FGNGTPVEE48 pKa = 4.43IKK50 pKa = 9.61STMLEE55 pKa = 4.12HH56 pKa = 7.86SNGQPGNFKK65 pKa = 10.48VYY67 pKa = 10.38RR68 pKa = 11.84EE69 pKa = 3.97YY70 pKa = 10.82HH71 pKa = 5.35EE72 pKa = 3.98KK73 pKa = 10.53LRR75 pKa = 11.84RR76 pKa = 11.84HH77 pKa = 6.29DD78 pKa = 2.98GWYY81 pKa = 10.05CFVVYY86 pKa = 10.14RR87 pKa = 11.84PHH89 pKa = 6.53GRR91 pKa = 11.84SGLTIVKK98 pKa = 10.21DD99 pKa = 3.58KK100 pKa = 10.51MIRR103 pKa = 11.84ACDD106 pKa = 3.56LPLLQWHH113 pKa = 7.01GGGDD117 pKa = 3.67HH118 pKa = 7.56RR119 pKa = 11.84GTQQAKK125 pKa = 9.92IPIRR129 pKa = 11.84DD130 pKa = 3.26IFF132 pKa = 4.04

Molecular weight: 15.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4221

0

4221

1232325

30

1656

292.0

31.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.763 ± 0.057

0.731 ± 0.012

8.802 ± 0.053

9.437 ± 0.058

3.217 ± 0.025

8.299 ± 0.043

1.924 ± 0.021

4.282 ± 0.029

1.717 ± 0.021

8.81 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.706 ± 0.017

2.384 ± 0.023

4.664 ± 0.027

2.506 ± 0.027

6.545 ± 0.038

5.523 ± 0.032

6.255 ± 0.029

8.517 ± 0.038

1.153 ± 0.016

2.765 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski