Bacteriophage sp.

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 29 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H7BVF6|H7BVF6_9VIRU Uncharacterized protein OS=Bacteriophage sp. OX=38018 GN=2019_scaffold132_00094 PE=4 SV=1
MM1 pKa = 7.55WYY3 pKa = 10.37VYY5 pKa = 10.21TDD7 pKa = 3.62PTSGSQYY14 pKa = 10.66VFDD17 pKa = 4.33TSEE20 pKa = 3.8VMHH23 pKa = 6.77FKK25 pKa = 10.46TSFSFDD31 pKa = 3.3GVTGLPVQQILRR43 pKa = 11.84DD44 pKa = 4.12TISGASASQRR54 pKa = 11.84YY55 pKa = 7.49MNSLYY60 pKa = 10.76EE61 pKa = 4.3SGLTAKK67 pKa = 9.39ATLEE71 pKa = 4.04YY72 pKa = 10.37TGEE75 pKa = 4.19LNDD78 pKa = 3.8KK79 pKa = 10.81AKK81 pKa = 10.39EE82 pKa = 3.82ALVKK86 pKa = 10.72SFEE89 pKa = 4.53DD90 pKa = 3.81FGSGARR96 pKa = 11.84NTGKK100 pKa = 10.43IIPVPLGMKK109 pKa = 7.67LTPLDD114 pKa = 3.64IKK116 pKa = 11.16LSDD119 pKa = 3.56SQFFEE124 pKa = 4.3LKK126 pKa = 10.31KK127 pKa = 10.06YY128 pKa = 8.25TALQIAAAFGVKK140 pKa = 9.71PNQINDD146 pKa = 3.6YY147 pKa = 10.4SKK149 pKa = 10.95SSYY152 pKa = 11.28ANSEE156 pKa = 3.97LQQLSFYY163 pKa = 11.13VDD165 pKa = 3.13TEE167 pKa = 4.29LFVIKK172 pKa = 10.31QYY174 pKa = 10.56EE175 pKa = 3.9EE176 pKa = 4.05EE177 pKa = 4.25INYY180 pKa = 10.24KK181 pKa = 9.41MLTDD185 pKa = 4.18EE186 pKa = 4.42EE187 pKa = 4.43QDD189 pKa = 3.1DD190 pKa = 4.29GFYY193 pKa = 11.06YY194 pKa = 10.52KK195 pKa = 10.95YY196 pKa = 10.49NEE198 pKa = 4.11KK199 pKa = 10.81VLFRR203 pKa = 11.84TDD205 pKa = 3.02SKK207 pKa = 10.19TQMEE211 pKa = 4.39YY212 pKa = 11.08LKK214 pKa = 10.72NGVSGSIMKK223 pKa = 10.07PNEE226 pKa = 3.52ARR228 pKa = 11.84RR229 pKa = 11.84KK230 pKa = 9.88LDD232 pKa = 3.66LPDD235 pKa = 4.81GEE237 pKa = 5.86GGDD240 pKa = 3.77TLLANGSIVPLTMAGAAYY258 pKa = 10.07QKK260 pKa = 10.95GQIEE264 pKa = 4.2QEE266 pKa = 4.16EE267 pKa = 4.87TEE269 pKa = 4.48KK270 pKa = 11.03PEE272 pKa = 3.94QPEE275 pKa = 4.3EE276 pKa = 3.93EE277 pKa = 4.49TEE279 pKa = 4.22PDD281 pKa = 3.4TEE283 pKa = 4.41QPDD286 pKa = 3.89TTGQPDD292 pKa = 3.62EE293 pKa = 4.24TDD295 pKa = 3.13EE296 pKa = 5.91AEE298 pKa = 4.95DD299 pKa = 3.96EE300 pKa = 4.32EE301 pKa = 4.41EE302 pKa = 4.55QEE304 pKa = 4.4GGEE307 pKa = 4.12

Molecular weight: 34.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H7BVF0|H7BVF0_9VIRU Uncharacterized protein OS=Bacteriophage sp. OX=38018 GN=2019_scaffold132_00065 PE=4 SV=1
MM1 pKa = 7.42GKK3 pKa = 10.14KK4 pKa = 9.73SVNNLYY10 pKa = 10.79KK11 pKa = 10.75PMLEE15 pKa = 4.19HH16 pKa = 7.33SNVEE20 pKa = 4.28QKK22 pKa = 9.6FHH24 pKa = 6.86KK25 pKa = 9.78AAKK28 pKa = 9.9GKK30 pKa = 7.47TEE32 pKa = 3.98RR33 pKa = 11.84PDD35 pKa = 3.1VAVILEE41 pKa = 3.98PTNIQRR47 pKa = 11.84HH48 pKa = 4.44VKK50 pKa = 10.21NVIEE54 pKa = 4.05QLEE57 pKa = 4.23NTAPEE62 pKa = 5.42GYY64 pKa = 10.01DD65 pKa = 3.26VPHH68 pKa = 7.3PEE70 pKa = 4.37KK71 pKa = 9.9AWKK74 pKa = 9.12PSRR77 pKa = 11.84HH78 pKa = 5.32GKK80 pKa = 8.94VCINEE85 pKa = 3.98GTSRR89 pKa = 11.84KK90 pKa = 8.89VRR92 pKa = 11.84MIEE95 pKa = 3.81KK96 pKa = 9.67PRR98 pKa = 11.84YY99 pKa = 8.55NYY101 pKa = 9.96EE102 pKa = 3.9QVIHH106 pKa = 6.74HH107 pKa = 6.44IVVSACYY114 pKa = 9.98DD115 pKa = 2.95IFMKK119 pKa = 10.55GMYY122 pKa = 9.2EE123 pKa = 4.13FSCGSVPNRR132 pKa = 11.84GAHH135 pKa = 5.31YY136 pKa = 8.87GKK138 pKa = 10.44KK139 pKa = 10.42YY140 pKa = 8.75IEE142 pKa = 3.89RR143 pKa = 11.84WIQRR147 pKa = 11.84DD148 pKa = 3.55KK149 pKa = 11.51KK150 pKa = 8.71NCKK153 pKa = 9.57YY154 pKa = 9.85VLKK157 pKa = 10.08MDD159 pKa = 2.97IRR161 pKa = 11.84HH162 pKa = 5.61FFEE165 pKa = 5.89SVDD168 pKa = 3.64HH169 pKa = 7.2DD170 pKa = 4.43VLKK173 pKa = 11.0AWLKK177 pKa = 10.74KK178 pKa = 10.13KK179 pKa = 10.38IRR181 pKa = 11.84DD182 pKa = 3.38EE183 pKa = 4.2RR184 pKa = 11.84MLYY187 pKa = 9.97ILEE190 pKa = 4.83LIIDD194 pKa = 4.34GSEE197 pKa = 3.68VGLPLGFYY205 pKa = 9.61TSQWLSNFMLQPLDD219 pKa = 3.98HH220 pKa = 7.2FIKK223 pKa = 10.05EE224 pKa = 3.93QLKK227 pKa = 8.12AVHH230 pKa = 6.71YY231 pKa = 9.66IRR233 pKa = 11.84YY234 pKa = 7.55MDD236 pKa = 4.14DD237 pKa = 2.87MVVFGKK243 pKa = 10.11NKK245 pKa = 10.13KK246 pKa = 8.46EE247 pKa = 3.84LHH249 pKa = 6.18RR250 pKa = 11.84MQQEE254 pKa = 3.55IEE256 pKa = 3.91RR257 pKa = 11.84YY258 pKa = 9.42LRR260 pKa = 11.84EE261 pKa = 4.04KK262 pKa = 10.94FNLQMKK268 pKa = 9.27GNWQVFRR275 pKa = 11.84FDD277 pKa = 3.48YY278 pKa = 10.52TEE280 pKa = 3.94KK281 pKa = 10.03KK282 pKa = 7.4TGKK285 pKa = 10.06RR286 pKa = 11.84KK287 pKa = 9.52GRR289 pKa = 11.84PLDD292 pKa = 3.48FMGFQFYY299 pKa = 10.39HH300 pKa = 7.45DD301 pKa = 3.7KK302 pKa = 10.81TILRR306 pKa = 11.84EE307 pKa = 4.29SIMLSCTRR315 pKa = 11.84KK316 pKa = 9.28VNRR319 pKa = 11.84VAKK322 pKa = 10.0KK323 pKa = 10.54EE324 pKa = 3.97KK325 pKa = 8.97ITWYY329 pKa = 10.03DD330 pKa = 2.9ATAILSYY337 pKa = 9.84MGYY340 pKa = 10.76LSNTDD345 pKa = 3.25TYY347 pKa = 12.01DD348 pKa = 3.0MYY350 pKa = 11.21LQRR353 pKa = 11.84VKK355 pKa = 10.63PYY357 pKa = 11.34VNVKK361 pKa = 9.43KK362 pKa = 10.61LKK364 pKa = 10.48KK365 pKa = 9.67IVSKK369 pKa = 10.52HH370 pKa = 4.39SKK372 pKa = 8.58RR373 pKa = 11.84KK374 pKa = 8.75EE375 pKa = 3.98RR376 pKa = 11.84EE377 pKa = 3.37KK378 pKa = 10.62HH379 pKa = 4.49EE380 pKa = 4.08RR381 pKa = 11.84MEE383 pKa = 4.12RR384 pKa = 11.84SVRR387 pKa = 11.84NGGRR391 pKa = 11.84TAGGVRR397 pKa = 11.84HH398 pKa = 6.46NSITDD403 pKa = 3.36NGVSEE408 pKa = 4.32TQYY411 pKa = 10.76QEE413 pKa = 4.04SNEE416 pKa = 4.06RR417 pKa = 11.84GCRR420 pKa = 11.84RR421 pKa = 11.84EE422 pKa = 3.68EE423 pKa = 4.01SHH425 pKa = 7.1RR426 pKa = 11.84MAARR430 pKa = 11.84GAA432 pKa = 3.52

Molecular weight: 51.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

29

0

29

6670

36

1117

230.0

25.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.391 ± 0.995

1.874 ± 0.404

5.847 ± 0.548

7.721 ± 0.571

4.078 ± 0.369

6.492 ± 0.438

1.694 ± 0.339

5.847 ± 0.355

8.291 ± 0.663

7.286 ± 0.372

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.999 ± 0.207

4.798 ± 0.271

3.178 ± 0.466

3.568 ± 0.248

4.303 ± 0.73

6.672 ± 0.599

6.867 ± 0.883

6.117 ± 0.341

1.139 ± 0.155

3.838 ± 0.511
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski