Grosmannia clavigera (strain kw1407 / UAMH 11150) (Blue stain fungus) (Graphiocladiella clavigera)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Ophiostomatales; Ophiostomataceae; Grosmannia; Grosmannia clavigera

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8311 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0XL67|F0XL67_GROCL Amino acid permease OS=Grosmannia clavigera (strain kw1407 / UAMH 11150) OX=655863 GN=CMQ_8220 PE=4 SV=1
MM1 pKa = 7.16RR2 pKa = 11.84QEE4 pKa = 3.85IGEE7 pKa = 4.38GLVDD11 pKa = 3.79TPLVEE16 pKa = 4.24TASQLVQGGGVAGDD30 pKa = 3.44VFVRR34 pKa = 11.84SLLALWRR41 pKa = 11.84IAVVADD47 pKa = 4.8FSSVAITAPVSDD59 pKa = 4.79DD60 pKa = 3.51VASAEE65 pKa = 3.68IWCFVFCGLRR75 pKa = 11.84LIYY78 pKa = 10.28YY79 pKa = 8.94GVVGLINDD87 pKa = 4.76GYY89 pKa = 10.47DD90 pKa = 3.1KK91 pKa = 10.97KK92 pKa = 11.05FKK94 pKa = 10.85VYY96 pKa = 10.64AKK98 pKa = 10.03VVAGGADD105 pKa = 3.73LQVHH109 pKa = 7.17DD110 pKa = 5.4FMQQIGDD117 pKa = 4.03KK118 pKa = 10.89DD119 pKa = 3.97SLDD122 pKa = 3.61KK123 pKa = 11.36SDD125 pKa = 5.32GYY127 pKa = 10.23NTMANLALNMSFASEE142 pKa = 4.48SEE144 pKa = 4.07PCPGDD149 pKa = 3.38FSEE152 pKa = 5.33RR153 pKa = 11.84GYY155 pKa = 11.61GPDD158 pKa = 3.43CLYY161 pKa = 10.84EE162 pKa = 3.88QTVYY166 pKa = 7.72WTLRR170 pKa = 11.84DD171 pKa = 3.68DD172 pKa = 3.9QADD175 pKa = 3.41VFYY178 pKa = 11.29ANLEE182 pKa = 4.27SATGIPKK189 pKa = 10.43DD190 pKa = 3.77KK191 pKa = 10.74IGFGYY196 pKa = 8.57YY197 pKa = 9.73TDD199 pKa = 4.31IDD201 pKa = 3.84NCSGSGSKK209 pKa = 10.61VGDD212 pKa = 4.53GDD214 pKa = 3.86NCWNIDD220 pKa = 3.78YY221 pKa = 10.78EE222 pKa = 4.6LGAPFPNGYY231 pKa = 8.28GTDD234 pKa = 3.72DD235 pKa = 3.46VANPKK240 pKa = 10.11DD241 pKa = 3.55VAKK244 pKa = 10.57II245 pKa = 3.63

Molecular weight: 26.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0X9S3|F0X9S3_GROCL Benzoate 4-monooxygenase cytochrome p450 OS=Grosmannia clavigera (strain kw1407 / UAMH 11150) OX=655863 GN=CMQ_3395 PE=3 SV=1
MM1 pKa = 7.02QSAYY5 pKa = 10.72SAFSLAVPFPCGQAATHH22 pKa = 6.06AVVAFTCAALGSGACDD38 pKa = 3.59GQSMLLALGWKK49 pKa = 9.25KK50 pKa = 9.12RR51 pKa = 11.84TYY53 pKa = 11.32GNTLGPARR61 pKa = 11.84DD62 pKa = 3.61VARR65 pKa = 11.84IAGSEE70 pKa = 3.76ARR72 pKa = 11.84GDD74 pKa = 3.52GRR76 pKa = 11.84LVRR79 pKa = 11.84GLGRR83 pKa = 11.84AEE85 pKa = 3.57AVGRR89 pKa = 11.84RR90 pKa = 11.84GHH92 pKa = 6.38KK93 pKa = 10.21GSGSRR98 pKa = 11.84RR99 pKa = 11.84RR100 pKa = 11.84RR101 pKa = 11.84GGLGRR106 pKa = 11.84GKK108 pKa = 10.46GSKK111 pKa = 9.8EE112 pKa = 3.39ADD114 pKa = 2.93EE115 pKa = 4.98SGRR118 pKa = 11.84EE119 pKa = 3.86THH121 pKa = 6.82GDD123 pKa = 3.65GMVLWTRR130 pKa = 11.84GVYY133 pKa = 10.41CILLEE138 pKa = 4.16RR139 pKa = 4.92

Molecular weight: 14.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8311

0

8311

4531634

35

6207

545.3

59.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.425 ± 0.03

1.156 ± 0.009

5.921 ± 0.017

5.716 ± 0.026

3.408 ± 0.016

7.414 ± 0.021

2.367 ± 0.011

4.08 ± 0.016

3.918 ± 0.023

8.906 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.148 ± 0.009

3.13 ± 0.014

5.904 ± 0.025

4.023 ± 0.02

6.669 ± 0.025

8.318 ± 0.033

6.053 ± 0.018

6.577 ± 0.021

1.352 ± 0.009

2.516 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski