Gordonia phage Bachita

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Smoothievirus; Gordonia virus Bachita

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 182 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A166YC14|A0A166YC14_9CAUD Uncharacterized protein OS=Gordonia phage Bachita OX=1838061 GN=189 PE=4 SV=1
MM1 pKa = 6.51MTLNEE6 pKa = 4.1YY7 pKa = 10.37HH8 pKa = 6.8VICADD13 pKa = 3.57GTSMNVEE20 pKa = 3.47AWGPADD26 pKa = 3.36AAEE29 pKa = 4.07RR30 pKa = 11.84VYY32 pKa = 10.75DD33 pKa = 3.68TDD35 pKa = 4.61RR36 pKa = 11.84DD37 pKa = 3.96GLQSEE42 pKa = 4.52IVAIIEE48 pKa = 3.88TEE50 pKa = 4.16YY51 pKa = 11.38AHH53 pKa = 6.43VNSKK57 pKa = 9.65IADD60 pKa = 4.0MIVSAVNLDD69 pKa = 3.79LPFVLPANHH78 pKa = 6.9

Molecular weight: 8.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A160DFR1|A0A160DFR1_9CAUD Uncharacterized protein OS=Gordonia phage Bachita OX=1838061 GN=123 PE=4 SV=1
MM1 pKa = 7.5TSGFAPSDD9 pKa = 3.46RR10 pKa = 11.84SVGRR14 pKa = 11.84PTSGLHH20 pKa = 5.72NLTAFGFAHH29 pKa = 7.0RR30 pKa = 11.84ASARR34 pKa = 11.84SRR36 pKa = 11.84AEE38 pKa = 3.68RR39 pKa = 11.84ALGRR43 pKa = 11.84LPWARR48 pKa = 11.84VGGRR52 pKa = 11.84AWAGARR58 pKa = 11.84GRR60 pKa = 11.84ARR62 pKa = 11.84VGGRR66 pKa = 11.84AWAGARR72 pKa = 11.84GRR74 pKa = 11.84ARR76 pKa = 11.84PRR78 pKa = 11.84ARR80 pKa = 11.84RR81 pKa = 11.84HH82 pKa = 5.43CPNRR86 pKa = 11.84ANQRR90 pKa = 11.84CHH92 pKa = 6.41GLGFPKK98 pKa = 10.24PAGAWW103 pKa = 3.28

Molecular weight: 11.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

182

0

182

29574

31

2931

162.5

18.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.552 ± 0.387

0.994 ± 0.124

7.104 ± 0.194

6.824 ± 0.224

3.206 ± 0.108

7.811 ± 0.235

2.337 ± 0.171

4.808 ± 0.159

4.108 ± 0.167

7.584 ± 0.179

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.522 ± 0.093

3.621 ± 0.148

4.866 ± 0.175

3.615 ± 0.245

7.091 ± 0.22

5.677 ± 0.143

5.894 ± 0.2

7.351 ± 0.251

2.188 ± 0.114

2.847 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski