Grasshopper associated circular virus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykolovirus; Gemykolovirus easlu1

Average proteome isoelectric point is 7.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346BP63|A0A346BP63_9VIRU Putative capsid protein OS=Grasshopper associated circular virus 1 OX=2293293 PE=4 SV=1
MM1 pKa = 6.92VRR3 pKa = 11.84KK4 pKa = 9.98FKK6 pKa = 10.86LDD8 pKa = 3.21NVNYY12 pKa = 10.36VLLTYY17 pKa = 10.46SDD19 pKa = 4.65CPNDD23 pKa = 4.17FDD25 pKa = 4.18PQLIIDD31 pKa = 4.19AVVGTGAVYY40 pKa = 10.28RR41 pKa = 11.84LGRR44 pKa = 11.84EE45 pKa = 3.54LHH47 pKa = 6.92ANGKK51 pKa = 7.8PHH53 pKa = 6.25FHH55 pKa = 7.14CFVQWAEE62 pKa = 4.28PFSHH66 pKa = 7.62PDD68 pKa = 2.97AGSLFFVGGRR78 pKa = 11.84RR79 pKa = 11.84ANIKK83 pKa = 10.19RR84 pKa = 11.84FSANPGRR91 pKa = 11.84RR92 pKa = 11.84WDD94 pKa = 3.79YY95 pKa = 8.01VGKK98 pKa = 10.45YY99 pKa = 9.04AGHH102 pKa = 6.91KK103 pKa = 9.66AGHH106 pKa = 6.62FIIGDD111 pKa = 3.44QCDD114 pKa = 3.52RR115 pKa = 11.84PGGDD119 pKa = 3.56KK120 pKa = 10.72DD121 pKa = 4.04DD122 pKa = 4.47SEE124 pKa = 4.46RR125 pKa = 11.84TQTDD129 pKa = 2.27IWSEE133 pKa = 3.94IINAEE138 pKa = 4.1TEE140 pKa = 4.25SEE142 pKa = 4.24FFEE145 pKa = 4.71KK146 pKa = 10.56LASLAPKK153 pKa = 10.27QLGCNFGSLKK163 pKa = 10.7LYY165 pKa = 10.78ADD167 pKa = 3.64WRR169 pKa = 11.84YY170 pKa = 10.03KK171 pKa = 10.78PPVTEE176 pKa = 4.47YY177 pKa = 10.35ISPSGNFYY185 pKa = 8.47PTTALADD192 pKa = 3.62WEE194 pKa = 4.3RR195 pKa = 11.84EE196 pKa = 3.9QLNSEE201 pKa = 3.97MSACLRR207 pKa = 11.84GGPLATPSPSASRR220 pKa = 11.84AGPHH224 pKa = 6.43RR225 pKa = 11.84LLPKK229 pKa = 10.5HH230 pKa = 5.54MLILEE235 pKa = 4.88FIRR238 pKa = 11.84PRR240 pKa = 11.84GLVLFGATRR249 pKa = 11.84LGKK252 pKa = 7.65TVWARR257 pKa = 11.84SLGTHH262 pKa = 6.35SYY264 pKa = 10.87FGGLFNMEE272 pKa = 4.71LFAEE276 pKa = 4.55EE277 pKa = 3.3ARR279 pKa = 11.84YY280 pKa = 9.95AIFDD284 pKa = 5.01DD285 pKa = 3.55ISGGFGFFPSYY296 pKa = 10.73KK297 pKa = 9.81LWLGGQFEE305 pKa = 4.69FSVTDD310 pKa = 3.54KK311 pKa = 11.09YY312 pKa = 10.68KK313 pKa = 10.67HH314 pKa = 5.7KK315 pKa = 11.09RR316 pKa = 11.84NVKK319 pKa = 8.0WGKK322 pKa = 8.34PAIWLCNTDD331 pKa = 5.46PRR333 pKa = 11.84LDD335 pKa = 3.32WYY337 pKa = 11.08KK338 pKa = 10.71PGTGPDD344 pKa = 3.99FEE346 pKa = 4.64WMEE349 pKa = 4.2NNCDD353 pKa = 3.33FVEE356 pKa = 3.99ITEE359 pKa = 4.77PIFRR363 pKa = 11.84ANTTT367 pKa = 3.29

Molecular weight: 41.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346BP63|A0A346BP63_9VIRU Putative capsid protein OS=Grasshopper associated circular virus 1 OX=2293293 PE=4 SV=1
MM1 pKa = 7.99AYY3 pKa = 9.8RR4 pKa = 11.84RR5 pKa = 11.84STRR8 pKa = 11.84RR9 pKa = 11.84YY10 pKa = 7.51AARR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84PTRR18 pKa = 11.84KK19 pKa = 8.63RR20 pKa = 11.84SYY22 pKa = 9.16PSRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84YY28 pKa = 7.07TGKK31 pKa = 9.99RR32 pKa = 11.84RR33 pKa = 11.84STSRR37 pKa = 11.84KK38 pKa = 5.12MTTKK42 pKa = 10.25RR43 pKa = 11.84VLNVTSRR50 pKa = 11.84KK51 pKa = 9.89KK52 pKa = 10.33QDD54 pKa = 2.85TMQNLTNYY62 pKa = 9.68SAASVSGGTQFYY74 pKa = 10.58SDD76 pKa = 3.43NTIISGGTLFASLWCPTARR95 pKa = 11.84TNHH98 pKa = 6.05AQLQAKK104 pKa = 7.75DD105 pKa = 3.48QPEE108 pKa = 4.02ILGGLFDD115 pKa = 4.04KK116 pKa = 10.33ATRR119 pKa = 11.84TASTCYY125 pKa = 9.67MRR127 pKa = 11.84GLKK130 pKa = 10.0EE131 pKa = 4.21KK132 pKa = 10.51IQIQTNTGVAWQWRR146 pKa = 11.84RR147 pKa = 11.84ICFTAKK153 pKa = 10.11GGSYY157 pKa = 10.22YY158 pKa = 11.27NGMTDD163 pKa = 3.1NAKK166 pKa = 10.23LVAFAGTGWARR177 pKa = 11.84VTSNWLFSDD186 pKa = 4.22PSKK189 pKa = 10.84QALFGPLFRR198 pKa = 11.84GQEE201 pKa = 4.17GVDD204 pKa = 2.85WRR206 pKa = 11.84NYY208 pKa = 9.36FSAPLDD214 pKa = 3.52THH216 pKa = 6.9RR217 pKa = 11.84MNVKK221 pKa = 9.69YY222 pKa = 10.18DD223 pKa = 3.32KK224 pKa = 10.87TVIIQSGNDD233 pKa = 3.21SGVMRR238 pKa = 11.84NYY240 pKa = 10.32SRR242 pKa = 11.84SPWTPTAA249 pKa = 3.83

Molecular weight: 28.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

616

249

367

308.0

35.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.468 ± 0.377

1.623 ± 0.279

5.195 ± 0.787

3.896 ± 1.797

5.844 ± 1.489

8.604 ± 0.65

1.948 ± 0.764

3.896 ± 0.456

5.682 ± 0.229

6.981 ± 1.175

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.948 ± 0.576

4.708 ± 0.343

5.357 ± 1.163

3.084 ± 1.158

8.279 ± 1.98

6.981 ± 1.238

7.305 ± 2.362

4.058 ± 0.028

2.922 ± 0.074

4.221 ± 0.4
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski