Streptococcus phage Javan597

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AL36|A0A4D6AL36_9CAUD Portal protein OS=Streptococcus phage Javan597 OX=2548279 GN=Javan597_0042 PE=4 SV=1
MM1 pKa = 7.27ITIQLDD7 pKa = 3.89EE8 pKa = 4.65EE9 pKa = 4.81LLTALVFAAAQSSCGFNRR27 pKa = 11.84NTLQEE32 pKa = 3.92NQLWHH37 pKa = 6.24LHH39 pKa = 5.53CCDD42 pKa = 3.53YY43 pKa = 11.38NEE45 pKa = 4.47PVYY48 pKa = 10.62EE49 pKa = 4.08VAKK52 pKa = 10.53QINLDD57 pKa = 4.36DD58 pKa = 4.5IQDD61 pKa = 3.56EE62 pKa = 4.66SYY64 pKa = 10.49RR65 pKa = 11.84AYY67 pKa = 10.33FQEE70 pKa = 4.42VKK72 pKa = 10.88AKK74 pKa = 8.88GDD76 pKa = 3.44KK77 pKa = 10.52YY78 pKa = 10.61FSEE81 pKa = 4.44VEE83 pKa = 4.12EE84 pKa = 4.38NEE86 pKa = 4.22KK87 pKa = 11.01QNN89 pKa = 3.65

Molecular weight: 10.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AK65|A0A4D6AK65_9CAUD Uncharacterized protein OS=Streptococcus phage Javan597 OX=2548279 GN=Javan597_0025 PE=4 SV=1
MM1 pKa = 7.35ATKK4 pKa = 10.43QPIRR8 pKa = 11.84DD9 pKa = 3.53LKK11 pKa = 10.88DD12 pKa = 2.95IQRR15 pKa = 11.84MKK17 pKa = 10.83DD18 pKa = 3.6YY19 pKa = 10.74LIHH22 pKa = 7.67DD23 pKa = 4.01SAKK26 pKa = 10.77NPVLRR31 pKa = 11.84LRR33 pKa = 11.84NYY35 pKa = 8.34TLFVTGINSGLRR47 pKa = 11.84MGDD50 pKa = 2.94IRR52 pKa = 11.84DD53 pKa = 3.93LKK55 pKa = 10.98VKK57 pKa = 10.48DD58 pKa = 3.4VTGWRR63 pKa = 11.84IKK65 pKa = 10.86HH66 pKa = 5.92FDD68 pKa = 3.4EE69 pKa = 4.46KK70 pKa = 10.41TGKK73 pKa = 8.6FTDD76 pKa = 3.27RR77 pKa = 11.84KK78 pKa = 9.58MNSSLKK84 pKa = 10.09KK85 pKa = 10.42AIRR88 pKa = 11.84NYY90 pKa = 10.99LGITKK95 pKa = 10.31LKK97 pKa = 10.79NEE99 pKa = 4.97DD100 pKa = 3.52YY101 pKa = 10.88LFPGSFKK108 pKa = 10.64QNRR111 pKa = 11.84KK112 pKa = 8.1MSEE115 pKa = 3.84SQAWRR120 pKa = 11.84IVTSAANFLGIPEE133 pKa = 4.68IGTHH137 pKa = 6.01SMRR140 pKa = 11.84KK141 pKa = 7.12TFGFQIFTTQGNKK154 pKa = 7.94TVGDD158 pKa = 3.44IMKK161 pKa = 10.32LLNHH165 pKa = 5.95QKK167 pKa = 10.74EE168 pKa = 4.72STTLAYY174 pKa = 9.64IGVTRR179 pKa = 11.84DD180 pKa = 3.62SEE182 pKa = 4.53DD183 pKa = 3.26KK184 pKa = 11.0TVDD187 pKa = 3.4KK188 pKa = 11.49LNLL191 pKa = 3.5

Molecular weight: 21.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

11934

37

1194

183.6

20.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.131 ± 0.554

0.587 ± 0.115

6.058 ± 0.333

7.567 ± 0.454

4.232 ± 0.199

6.36 ± 0.494

1.575 ± 0.189

6.838 ± 0.17

7.826 ± 0.412

8.187 ± 0.393

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.497 ± 0.17

5.581 ± 0.256

3.05 ± 0.222

3.905 ± 0.231

4.491 ± 0.298

6.209 ± 0.421

6.109 ± 0.452

6.519 ± 0.215

1.559 ± 0.181

3.72 ± 0.309
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski