Amino acid dipepetide frequency for Flavobacterium phage FPSV-D15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.509AlaAla: 2.509 ± 0.671
0.279AlaCys: 0.279 ± 0.154
3.067AlaAsp: 3.067 ± 0.587
4.112AlaGlu: 4.112 ± 0.643
3.415AlaPhe: 3.415 ± 0.391
4.043AlaGly: 4.043 ± 0.691
0.767AlaHis: 0.767 ± 0.224
5.228AlaIle: 5.228 ± 0.623
4.949AlaLys: 4.949 ± 1.044
5.158AlaLeu: 5.158 ± 0.727
0.976AlaMet: 0.976 ± 0.224
4.043AlaAsn: 4.043 ± 0.53
1.046AlaPro: 1.046 ± 0.276
2.509AlaGln: 2.509 ± 0.642
1.743AlaArg: 1.743 ± 0.318
3.624AlaSer: 3.624 ± 0.611
3.276AlaThr: 3.276 ± 0.545
2.649AlaVal: 2.649 ± 0.401
0.627AlaTrp: 0.627 ± 0.213
2.37AlaTyr: 2.37 ± 0.34
0.0AlaXaa: 0.0 ± 0.0
Cys
0.209CysAla: 0.209 ± 0.104
0.209CysCys: 0.209 ± 0.125
0.767CysAsp: 0.767 ± 0.257
0.279CysGlu: 0.279 ± 0.19
0.349CysPhe: 0.349 ± 0.137
0.279CysGly: 0.279 ± 0.124
0.209CysHis: 0.209 ± 0.115
0.139CysIle: 0.139 ± 0.108
0.767CysLys: 0.767 ± 0.296
0.418CysLeu: 0.418 ± 0.207
0.209CysMet: 0.209 ± 0.115
0.349CysAsn: 0.349 ± 0.209
0.349CysPro: 0.349 ± 0.158
0.349CysGln: 0.349 ± 0.142
0.279CysArg: 0.279 ± 0.163
0.558CysSer: 0.558 ± 0.178
0.488CysThr: 0.488 ± 0.165
0.488CysVal: 0.488 ± 0.164
0.0CysTrp: 0.0 ± 0.0
0.558CysTyr: 0.558 ± 0.214
0.0CysXaa: 0.0 ± 0.0
Asp
4.043AspAla: 4.043 ± 0.51
0.558AspCys: 0.558 ± 0.207
2.788AspAsp: 2.788 ± 0.515
4.321AspGlu: 4.321 ± 0.577
3.206AspPhe: 3.206 ± 0.505
2.997AspGly: 2.997 ± 0.466
0.627AspHis: 0.627 ± 0.231
4.74AspIle: 4.74 ± 0.542
5.158AspLys: 5.158 ± 0.44
5.158AspLeu: 5.158 ± 0.672
1.115AspMet: 1.115 ± 0.281
2.788AspAsn: 2.788 ± 0.374
0.906AspPro: 0.906 ± 0.227
1.464AspGln: 1.464 ± 0.272
2.23AspArg: 2.23 ± 0.393
3.694AspSer: 3.694 ± 0.662
2.509AspThr: 2.509 ± 0.541
3.346AspVal: 3.346 ± 0.446
0.976AspTrp: 0.976 ± 0.266
3.346AspTyr: 3.346 ± 0.621
0.0AspXaa: 0.0 ± 0.0
Glu
3.903GluAla: 3.903 ± 0.454
0.627GluCys: 0.627 ± 0.214
3.694GluAsp: 3.694 ± 0.548
5.158GluGlu: 5.158 ± 0.6
2.997GluPhe: 2.997 ± 0.429
3.903GluGly: 3.903 ± 0.5
1.255GluHis: 1.255 ± 0.271
7.946GluIle: 7.946 ± 0.887
7.11GluLys: 7.11 ± 0.662
8.155GluLeu: 8.155 ± 0.883
1.673GluMet: 1.673 ± 0.295
5.646GluAsn: 5.646 ± 0.673
1.255GluPro: 1.255 ± 0.341
2.788GluGln: 2.788 ± 0.422
2.997GluArg: 2.997 ± 0.486
3.694GluSer: 3.694 ± 0.507
2.927GluThr: 2.927 ± 0.408
4.809GluVal: 4.809 ± 0.649
0.767GluTrp: 0.767 ± 0.247
3.067GluTyr: 3.067 ± 0.46
0.0GluXaa: 0.0 ± 0.0
Phe
2.021PheAla: 2.021 ± 0.41
0.488PheCys: 0.488 ± 0.139
3.206PheAsp: 3.206 ± 0.434
5.018PheGlu: 5.018 ± 0.781
2.161PhePhe: 2.161 ± 0.406
2.509PheGly: 2.509 ± 0.502
0.697PheHis: 0.697 ± 0.214
3.973PheIle: 3.973 ± 0.616
3.834PheLys: 3.834 ± 0.486
4.112PheLeu: 4.112 ± 0.641
0.906PheMet: 0.906 ± 0.293
2.579PheAsn: 2.579 ± 0.433
1.533PhePro: 1.533 ± 0.352
1.882PheGln: 1.882 ± 0.389
2.718PheArg: 2.718 ± 0.427
3.555PheSer: 3.555 ± 0.494
2.3PheThr: 2.3 ± 0.405
2.509PheVal: 2.509 ± 0.427
1.046PheTrp: 1.046 ± 0.242
2.649PheTyr: 2.649 ± 0.505
0.0PheXaa: 0.0 ± 0.0
Gly
2.788GlyAla: 2.788 ± 0.605
0.349GlyCys: 0.349 ± 0.174
2.091GlyAsp: 2.091 ± 0.401
2.997GlyGlu: 2.997 ± 0.504
2.927GlyPhe: 2.927 ± 0.433
2.3GlyGly: 2.3 ± 0.479
0.558GlyHis: 0.558 ± 0.179
4.67GlyIle: 4.67 ± 0.542
4.043GlyLys: 4.043 ± 0.686
4.531GlyLeu: 4.531 ± 0.563
1.533GlyMet: 1.533 ± 0.329
3.764GlyAsn: 3.764 ± 0.528
0.697GlyPro: 0.697 ± 0.208
1.464GlyGln: 1.464 ± 0.323
1.743GlyArg: 1.743 ± 0.422
2.927GlySer: 2.927 ± 0.439
3.137GlyThr: 3.137 ± 0.34
3.346GlyVal: 3.346 ± 0.657
0.976GlyTrp: 0.976 ± 0.233
2.649GlyTyr: 2.649 ± 0.41
0.0GlyXaa: 0.0 ± 0.0
His
0.488HisAla: 0.488 ± 0.187
0.07HisCys: 0.07 ± 0.075
0.697HisAsp: 0.697 ± 0.2
0.976HisGlu: 0.976 ± 0.285
1.046HisPhe: 1.046 ± 0.34
1.046HisGly: 1.046 ± 0.244
0.209HisHis: 0.209 ± 0.109
1.743HisIle: 1.743 ± 0.365
1.673HisLys: 1.673 ± 0.385
1.952HisLeu: 1.952 ± 0.391
0.279HisMet: 0.279 ± 0.109
1.115HisAsn: 1.115 ± 0.224
0.418HisPro: 0.418 ± 0.16
0.418HisGln: 0.418 ± 0.136
0.836HisArg: 0.836 ± 0.239
0.976HisSer: 0.976 ± 0.249
0.836HisThr: 0.836 ± 0.192
0.418HisVal: 0.418 ± 0.153
0.07HisTrp: 0.07 ± 0.066
0.836HisTyr: 0.836 ± 0.205
0.0HisXaa: 0.0 ± 0.0
Ile
5.646IleAla: 5.646 ± 0.885
0.558IleCys: 0.558 ± 0.234
5.367IleAsp: 5.367 ± 0.562
8.782IleGlu: 8.782 ± 0.64
4.182IlePhe: 4.182 ± 0.504
2.858IleGly: 2.858 ± 0.385
2.021IleHis: 2.021 ± 0.378
5.437IleIle: 5.437 ± 0.588
9.131IleLys: 9.131 ± 0.683
6.691IleLeu: 6.691 ± 0.521
2.3IleMet: 2.3 ± 0.425
6.203IleAsn: 6.203 ± 0.603
2.718IlePro: 2.718 ± 0.337
3.415IleGln: 3.415 ± 0.489
3.206IleArg: 3.206 ± 0.579
5.925IleSer: 5.925 ± 0.634
4.321IleThr: 4.321 ± 0.642
4.461IleVal: 4.461 ± 0.563
0.558IleTrp: 0.558 ± 0.219
3.206IleTyr: 3.206 ± 0.47
0.0IleXaa: 0.0 ± 0.0
Lys
5.715LysAla: 5.715 ± 0.766
0.279LysCys: 0.279 ± 0.149
4.112LysAsp: 4.112 ± 0.409
7.597LysGlu: 7.597 ± 0.816
3.764LysPhe: 3.764 ± 0.542
3.764LysGly: 3.764 ± 0.478
1.394LysHis: 1.394 ± 0.269
9.34LysIle: 9.34 ± 0.793
9.27LysLys: 9.27 ± 1.031
8.364LysLeu: 8.364 ± 0.931
2.44LysMet: 2.44 ± 0.415
7.179LysAsn: 7.179 ± 0.659
2.579LysPro: 2.579 ± 0.35
3.415LysGln: 3.415 ± 0.545
4.112LysArg: 4.112 ± 0.545
6.273LysSer: 6.273 ± 0.519
5.437LysThr: 5.437 ± 0.56
5.646LysVal: 5.646 ± 0.492
1.185LysTrp: 1.185 ± 0.292
4.67LysTyr: 4.67 ± 0.564
0.0LysXaa: 0.0 ± 0.0
Leu
5.367LeuAla: 5.367 ± 0.471
0.767LeuCys: 0.767 ± 0.234
4.809LeuAsp: 4.809 ± 0.584
5.925LeuGlu: 5.925 ± 0.463
4.112LeuPhe: 4.112 ± 0.596
4.112LeuGly: 4.112 ± 0.609
1.394LeuHis: 1.394 ± 0.308
6.691LeuIle: 6.691 ± 0.71
9.479LeuLys: 9.479 ± 0.714
8.225LeuLeu: 8.225 ± 0.788
2.021LeuMet: 2.021 ± 0.378
6.203LeuAsn: 6.203 ± 0.764
3.764LeuPro: 3.764 ± 0.622
4.321LeuGln: 4.321 ± 0.42
3.624LeuArg: 3.624 ± 0.533
6.273LeuSer: 6.273 ± 0.653
4.949LeuThr: 4.949 ± 0.675
4.112LeuVal: 4.112 ± 0.434
1.046LeuTrp: 1.046 ± 0.23
2.858LeuTyr: 2.858 ± 0.355
0.0LeuXaa: 0.0 ± 0.0
Met
1.882MetAla: 1.882 ± 0.347
0.139MetCys: 0.139 ± 0.134
1.533MetAsp: 1.533 ± 0.269
1.324MetGlu: 1.324 ± 0.318
0.836MetPhe: 0.836 ± 0.226
0.697MetGly: 0.697 ± 0.181
0.279MetHis: 0.279 ± 0.149
1.046MetIle: 1.046 ± 0.218
2.858MetLys: 2.858 ± 0.39
2.161MetLeu: 2.161 ± 0.477
0.767MetMet: 0.767 ± 0.271
1.603MetAsn: 1.603 ± 0.265
0.697MetPro: 0.697 ± 0.209
1.046MetGln: 1.046 ± 0.259
0.836MetArg: 0.836 ± 0.221
1.603MetSer: 1.603 ± 0.301
1.812MetThr: 1.812 ± 0.307
1.115MetVal: 1.115 ± 0.337
0.279MetTrp: 0.279 ± 0.135
0.836MetTyr: 0.836 ± 0.297
0.0MetXaa: 0.0 ± 0.0
Asn
4.112AsnAla: 4.112 ± 0.625
0.418AsnCys: 0.418 ± 0.169
4.112AsnAsp: 4.112 ± 0.591
4.949AsnGlu: 4.949 ± 0.596
3.346AsnPhe: 3.346 ± 0.613
3.973AsnGly: 3.973 ± 0.516
1.046AsnHis: 1.046 ± 0.246
6.273AsnIle: 6.273 ± 0.824
6.064AsnLys: 6.064 ± 0.648
5.506AsnLeu: 5.506 ± 0.688
1.324AsnMet: 1.324 ± 0.277
4.321AsnAsn: 4.321 ± 0.653
1.882AsnPro: 1.882 ± 0.322
2.37AsnGln: 2.37 ± 0.449
3.346AsnArg: 3.346 ± 0.48
4.6AsnSer: 4.6 ± 0.529
3.764AsnThr: 3.764 ± 0.55
3.834AsnVal: 3.834 ± 0.522
0.627AsnTrp: 0.627 ± 0.23
2.718AsnTyr: 2.718 ± 0.456
0.0AsnXaa: 0.0 ± 0.0
Pro
1.464ProAla: 1.464 ± 0.351
0.209ProCys: 0.209 ± 0.111
1.255ProAsp: 1.255 ± 0.253
2.579ProGlu: 2.579 ± 0.414
1.533ProPhe: 1.533 ± 0.388
1.046ProGly: 1.046 ± 0.249
0.279ProHis: 0.279 ± 0.117
2.161ProIle: 2.161 ± 0.32
2.44ProLys: 2.44 ± 0.44
2.3ProLeu: 2.3 ± 0.397
0.558ProMet: 0.558 ± 0.205
2.44ProAsn: 2.44 ± 0.29
0.976ProPro: 0.976 ± 0.249
0.976ProGln: 0.976 ± 0.249
0.627ProArg: 0.627 ± 0.197
2.021ProSer: 2.021 ± 0.381
1.464ProThr: 1.464 ± 0.355
2.23ProVal: 2.23 ± 0.608
0.279ProTrp: 0.279 ± 0.115
1.115ProTyr: 1.115 ± 0.299
0.0ProXaa: 0.0 ± 0.0
Gln
2.23GlnAla: 2.23 ± 0.511
0.279GlnCys: 0.279 ± 0.14
1.952GlnAsp: 1.952 ± 0.346
3.485GlnGlu: 3.485 ± 0.474
1.394GlnPhe: 1.394 ± 0.297
1.533GlnGly: 1.533 ± 0.264
0.697GlnHis: 0.697 ± 0.175
3.624GlnIle: 3.624 ± 0.565
4.043GlnLys: 4.043 ± 0.52
3.555GlnLeu: 3.555 ± 0.383
1.046GlnMet: 1.046 ± 0.238
2.091GlnAsn: 2.091 ± 0.334
0.976GlnPro: 0.976 ± 0.249
1.882GlnGln: 1.882 ± 0.375
1.394GlnArg: 1.394 ± 0.262
1.394GlnSer: 1.394 ± 0.275
2.44GlnThr: 2.44 ± 0.399
1.743GlnVal: 1.743 ± 0.394
0.488GlnTrp: 0.488 ± 0.205
1.394GlnTyr: 1.394 ± 0.278
0.0GlnXaa: 0.0 ± 0.0
Arg
2.3ArgAla: 2.3 ± 0.38
0.558ArgCys: 0.558 ± 0.186
2.579ArgAsp: 2.579 ± 0.487
2.788ArgGlu: 2.788 ± 0.418
2.161ArgPhe: 2.161 ± 0.368
1.812ArgGly: 1.812 ± 0.248
0.767ArgHis: 0.767 ± 0.25
3.834ArgIle: 3.834 ± 0.374
4.182ArgLys: 4.182 ± 0.416
3.206ArgLeu: 3.206 ± 0.355
0.697ArgMet: 0.697 ± 0.254
2.37ArgAsn: 2.37 ± 0.341
0.697ArgPro: 0.697 ± 0.192
1.464ArgGln: 1.464 ± 0.287
1.185ArgArg: 1.185 ± 0.324
1.882ArgSer: 1.882 ± 0.438
1.464ArgThr: 1.464 ± 0.302
2.509ArgVal: 2.509 ± 0.388
0.209ArgTrp: 0.209 ± 0.146
0.836ArgTyr: 0.836 ± 0.248
0.0ArgXaa: 0.0 ± 0.0
Ser
3.346SerAla: 3.346 ± 0.415
0.488SerCys: 0.488 ± 0.188
4.461SerAsp: 4.461 ± 0.384
4.182SerGlu: 4.182 ± 0.537
4.252SerPhe: 4.252 ± 0.574
3.834SerGly: 3.834 ± 0.622
0.836SerHis: 0.836 ± 0.255
5.297SerIle: 5.297 ± 0.55
6.064SerLys: 6.064 ± 0.663
5.297SerLeu: 5.297 ± 0.552
1.952SerMet: 1.952 ± 0.477
3.973SerAsn: 3.973 ± 0.468
1.952SerPro: 1.952 ± 0.303
2.44SerGln: 2.44 ± 0.452
1.743SerArg: 1.743 ± 0.348
3.903SerSer: 3.903 ± 0.574
3.346SerThr: 3.346 ± 0.437
3.485SerVal: 3.485 ± 0.467
0.488SerTrp: 0.488 ± 0.17
2.021SerTyr: 2.021 ± 0.387
0.0SerXaa: 0.0 ± 0.0
Thr
3.067ThrAla: 3.067 ± 0.601
0.209ThrCys: 0.209 ± 0.131
3.206ThrAsp: 3.206 ± 0.512
3.694ThrGlu: 3.694 ± 0.469
2.788ThrPhe: 2.788 ± 0.439
3.973ThrGly: 3.973 ± 0.566
0.767ThrHis: 0.767 ± 0.203
5.297ThrIle: 5.297 ± 0.56
4.879ThrLys: 4.879 ± 0.569
4.321ThrLeu: 4.321 ± 0.442
0.906ThrMet: 0.906 ± 0.237
3.346ThrAsn: 3.346 ± 0.485
2.44ThrPro: 2.44 ± 0.444
1.603ThrGln: 1.603 ± 0.305
1.464ThrArg: 1.464 ± 0.338
2.858ThrSer: 2.858 ± 0.464
2.23ThrThr: 2.23 ± 0.362
2.23ThrVal: 2.23 ± 0.387
0.418ThrTrp: 0.418 ± 0.144
1.952ThrTyr: 1.952 ± 0.507
0.0ThrXaa: 0.0 ± 0.0
Val
3.276ValAla: 3.276 ± 0.836
0.488ValCys: 0.488 ± 0.183
3.415ValAsp: 3.415 ± 0.404
3.694ValGlu: 3.694 ± 0.448
2.649ValPhe: 2.649 ± 0.481
2.37ValGly: 2.37 ± 0.401
1.394ValHis: 1.394 ± 0.361
4.67ValIle: 4.67 ± 0.572
3.973ValLys: 3.973 ± 0.579
5.228ValLeu: 5.228 ± 0.537
1.533ValMet: 1.533 ± 0.433
4.321ValAsn: 4.321 ± 0.571
1.394ValPro: 1.394 ± 0.403
1.603ValGln: 1.603 ± 0.33
1.394ValArg: 1.394 ± 0.276
4.112ValSer: 4.112 ± 0.469
2.37ValThr: 2.37 ± 0.365
3.206ValVal: 3.206 ± 0.426
0.976ValTrp: 0.976 ± 0.252
2.161ValTyr: 2.161 ± 0.438
0.0ValXaa: 0.0 ± 0.0
Trp
0.627TrpAla: 0.627 ± 0.196
0.07TrpCys: 0.07 ± 0.069
0.836TrpAsp: 0.836 ± 0.334
0.558TrpGlu: 0.558 ± 0.208
0.349TrpPhe: 0.349 ± 0.124
0.558TrpGly: 0.558 ± 0.193
0.139TrpHis: 0.139 ± 0.092
1.046TrpIle: 1.046 ± 0.358
1.394TrpLys: 1.394 ± 0.346
1.743TrpLeu: 1.743 ± 0.377
0.279TrpMet: 0.279 ± 0.129
0.488TrpAsn: 0.488 ± 0.179
0.209TrpPro: 0.209 ± 0.11
0.558TrpGln: 0.558 ± 0.173
0.279TrpArg: 0.279 ± 0.124
0.488TrpSer: 0.488 ± 0.195
0.418TrpThr: 0.418 ± 0.127
0.836TrpVal: 0.836 ± 0.245
0.139TrpTrp: 0.139 ± 0.094
0.627TrpTyr: 0.627 ± 0.189
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.394TyrAla: 1.394 ± 0.28
0.209TyrCys: 0.209 ± 0.106
2.021TyrAsp: 2.021 ± 0.307
1.952TyrGlu: 1.952 ± 0.332
2.23TyrPhe: 2.23 ± 0.428
1.882TyrGly: 1.882 ± 0.374
0.767TyrHis: 0.767 ± 0.241
3.834TyrIle: 3.834 ± 0.618
5.228TyrLys: 5.228 ± 0.526
3.973TyrLeu: 3.973 ± 0.59
0.767TyrMet: 0.767 ± 0.214
3.764TyrAsn: 3.764 ± 0.532
1.464TyrPro: 1.464 ± 0.33
1.673TyrGln: 1.673 ± 0.318
1.812TyrArg: 1.812 ± 0.375
2.997TyrSer: 2.997 ± 0.451
2.161TyrThr: 2.161 ± 0.38
1.255TyrVal: 1.255 ± 0.338
0.488TyrTrp: 0.488 ± 0.17
1.394TyrTyr: 1.394 ± 0.365
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (14348 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski