Amycolatopsis marina

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6643 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I1CQQ6|A0A1I1CQQ6_9PSEU DDE superfamily endonuclease (Fragment) OS=Amycolatopsis marina OX=490629 GN=SAMN05216266_1341 PE=4 SV=1
GG1 pKa = 7.29DD2 pKa = 3.42QDD4 pKa = 5.33AEE6 pKa = 4.26GNYY9 pKa = 9.57IGSATVTVTATDD21 pKa = 3.56ADD23 pKa = 4.13SGVDD27 pKa = 3.37TVEE30 pKa = 3.91YY31 pKa = 10.55QLDD34 pKa = 3.59GGAYY38 pKa = 8.5QAYY41 pKa = 5.56TTPITVDD48 pKa = 2.93TAGEE52 pKa = 3.91HH53 pKa = 5.08TLQYY57 pKa = 10.77RR58 pKa = 11.84ATDD61 pKa = 3.4NSGNTSEE68 pKa = 4.33AGSVIFTVTEE78 pKa = 3.94PAPDD82 pKa = 4.55DD83 pKa = 3.59STAPEE88 pKa = 3.93VSGEE92 pKa = 4.04VSGDD96 pKa = 2.97QDD98 pKa = 4.45AEE100 pKa = 4.21GNYY103 pKa = 9.94VGSATVTVTATDD115 pKa = 3.69ADD117 pKa = 4.02SGLDD121 pKa = 3.5TVHH124 pKa = 6.76FAIDD128 pKa = 3.47GGSYY132 pKa = 10.71NPYY135 pKa = 8.88TEE137 pKa = 5.31PIVVSEE143 pKa = 4.3PGEE146 pKa = 4.08HH147 pKa = 4.89TVSFRR152 pKa = 11.84ATDD155 pKa = 3.49NAGNTSEE162 pKa = 4.09IASVSFTVVAEE173 pKa = 4.83DD174 pKa = 4.78PDD176 pKa = 4.12DD177 pKa = 3.9TAPPQVNAEE186 pKa = 4.11VTGDD190 pKa = 3.25QDD192 pKa = 4.86AEE194 pKa = 4.26GNYY197 pKa = 9.94VGSATVTLSASDD209 pKa = 3.35TGSGVFALRR218 pKa = 11.84YY219 pKa = 9.72SLDD222 pKa = 3.7GGSFTPYY229 pKa = 10.51DD230 pKa = 4.07DD231 pKa = 4.92PLVLTAPGEE240 pKa = 4.22HH241 pKa = 5.31TVLYY245 pKa = 10.25RR246 pKa = 11.84ATDD249 pKa = 3.31NAGNVSEE256 pKa = 4.46TGSLTFTVVASDD268 pKa = 3.95SDD270 pKa = 3.95ACPGSDD276 pKa = 3.3VRR278 pKa = 11.84EE279 pKa = 3.95TVIIGNNDD287 pKa = 3.11STVANVDD294 pKa = 3.36TGDD297 pKa = 3.45GCTINDD303 pKa = 4.85LIDD306 pKa = 3.86EE307 pKa = 4.48NGEE310 pKa = 4.07YY311 pKa = 10.68ANHH314 pKa = 6.02GKK316 pKa = 9.4FVKK319 pKa = 10.01HH320 pKa = 5.22VRR322 pKa = 11.84QVTDD326 pKa = 3.49ALVADD331 pKa = 5.79GIISDD336 pKa = 3.94QEE338 pKa = 3.79KK339 pKa = 10.33GRR341 pKa = 11.84IMNAAARR348 pKa = 11.84SDD350 pKa = 3.5VGKK353 pKa = 10.72

Molecular weight: 36.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I0XF82|A0A1I0XF82_9PSEU Two-component system OmpR family sensor histidine kinase SenX3 OS=Amycolatopsis marina OX=490629 GN=SAMN05216266_103167 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.71QGKK33 pKa = 8.65

Molecular weight: 4.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6643

0

6643

2101265

27

18730

316.3

33.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.959 ± 0.039

0.782 ± 0.011

5.982 ± 0.027

5.95 ± 0.032

2.831 ± 0.019

9.221 ± 0.028

2.229 ± 0.014

3.566 ± 0.019

1.942 ± 0.018

10.485 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.773 ± 0.013

1.981 ± 0.016

5.73 ± 0.031

2.925 ± 0.019

7.944 ± 0.033

5.385 ± 0.02

6.006 ± 0.021

8.817 ± 0.029

1.489 ± 0.013

1.999 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski