Banana bunchy top virus (BBTV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Mulpavirales; Nanoviridae; Babuvirus

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4A1I8|Q4A1I8_BBTV Master replication initiator protein OS=Banana bunchy top virus OX=12585 GN=rep PE=3 SV=1
MM1 pKa = 7.33EE2 pKa = 4.97FWEE5 pKa = 4.67SSAMPDD11 pKa = 3.64DD12 pKa = 3.95VKK14 pKa = 11.31RR15 pKa = 11.84EE16 pKa = 3.72IKK18 pKa = 10.1EE19 pKa = 4.02IYY21 pKa = 9.15WEE23 pKa = 4.07DD24 pKa = 3.32RR25 pKa = 11.84KK26 pKa = 11.05KK27 pKa = 11.13LLFCQKK33 pKa = 9.66LKK35 pKa = 10.9SYY37 pKa = 9.52VRR39 pKa = 11.84RR40 pKa = 11.84ILVYY44 pKa = 10.64GDD46 pKa = 3.7QEE48 pKa = 4.09DD49 pKa = 3.97ALAGVKK55 pKa = 9.73DD56 pKa = 4.52LKK58 pKa = 10.44TSIIRR63 pKa = 11.84YY64 pKa = 8.98SEE66 pKa = 4.17YY67 pKa = 10.68LKK69 pKa = 10.7KK70 pKa = 10.21PCVVICCVSNKK81 pKa = 10.3SIVYY85 pKa = 9.52RR86 pKa = 11.84LNSMVFFYY94 pKa = 10.84HH95 pKa = 7.31EE96 pKa = 4.24YY97 pKa = 10.77LEE99 pKa = 4.32EE100 pKa = 5.46LGGDD104 pKa = 3.39YY105 pKa = 10.77SVYY108 pKa = 10.45QDD110 pKa = 4.41LYY112 pKa = 9.79CDD114 pKa = 3.93EE115 pKa = 4.72VLSSSSTEE123 pKa = 3.93EE124 pKa = 3.64EE125 pKa = 4.28DD126 pKa = 3.54VGVIYY131 pKa = 10.6RR132 pKa = 11.84NVIMASTQEE141 pKa = 3.96KK142 pKa = 10.32FSWSDD147 pKa = 3.2CQKK150 pKa = 10.72IVISDD155 pKa = 3.75YY156 pKa = 11.63DD157 pKa = 3.64VTLLL161 pKa = 4.53

Molecular weight: 18.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9PWT9|Q9PWT9_BBTV Capsid protein OS=Banana bunchy top virus OX=12585 GN=cp PE=3 SV=1
MM1 pKa = 7.37ARR3 pKa = 11.84YY4 pKa = 7.71PKK6 pKa = 10.43KK7 pKa = 10.22SIKK10 pKa = 9.36KK11 pKa = 8.63RR12 pKa = 11.84RR13 pKa = 11.84VGRR16 pKa = 11.84RR17 pKa = 11.84KK18 pKa = 10.11YY19 pKa = 10.24GSKK22 pKa = 10.38AATSHH27 pKa = 7.34DD28 pKa = 4.11YY29 pKa = 11.37SSLGSILVPEE39 pKa = 4.2NTVKK43 pKa = 10.29VFRR46 pKa = 11.84IEE48 pKa = 4.17PTDD51 pKa = 3.37KK52 pKa = 10.24TLPRR56 pKa = 11.84YY57 pKa = 8.25FIWKK61 pKa = 8.52MFMLLVCKK69 pKa = 10.15VKK71 pKa = 10.32PGRR74 pKa = 11.84ILHH77 pKa = 5.87WAMIKK82 pKa = 10.45SSWEE86 pKa = 3.49INQPTTCLEE95 pKa = 4.07APGLFIKK102 pKa = 10.26PEE104 pKa = 3.95HH105 pKa = 5.73SHH107 pKa = 6.35LVKK110 pKa = 10.53LVCSGEE116 pKa = 4.15LEE118 pKa = 4.25AGVATGTSDD127 pKa = 4.34VEE129 pKa = 3.99CLLRR133 pKa = 11.84KK134 pKa = 6.9TTVLRR139 pKa = 11.84KK140 pKa = 9.69NVTEE144 pKa = 3.73VDD146 pKa = 3.52YY147 pKa = 11.13LYY149 pKa = 11.24LAFYY153 pKa = 9.92CSSGVSINYY162 pKa = 7.83QNRR165 pKa = 11.84ITYY168 pKa = 9.37HH169 pKa = 4.27VV170 pKa = 3.46

Molecular weight: 19.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

888

117

286

177.6

20.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.829 ± 0.401

3.041 ± 0.707

5.518 ± 1.044

7.207 ± 0.779

4.505 ± 0.727

5.856 ± 0.775

1.464 ± 0.454

6.194 ± 0.398

6.982 ± 0.967

7.432 ± 0.975

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.041 ± 0.528

3.829 ± 0.868

3.604 ± 0.604

3.153 ± 1.161

6.982 ± 0.95

7.432 ± 1.482

4.279 ± 0.819

8.221 ± 0.769

1.689 ± 0.108

5.743 ± 0.639
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski