Amino acid dipepetide frequency for Actinobacillus delphinicola

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.556AlaAla: 6.556 ± 0.183
0.899AlaCys: 0.899 ± 0.042
4.36AlaAsp: 4.36 ± 0.108
5.321AlaGlu: 5.321 ± 0.144
3.355AlaPhe: 3.355 ± 0.112
5.49AlaGly: 5.49 ± 0.171
1.508AlaHis: 1.508 ± 0.05
6.383AlaIle: 6.383 ± 0.192
6.046AlaLys: 6.046 ± 0.17
9.293AlaLeu: 9.293 ± 0.139
2.404AlaMet: 2.404 ± 0.073
3.907AlaAsn: 3.907 ± 0.134
2.867AlaPro: 2.867 ± 0.087
4.257AlaGln: 4.257 ± 0.124
3.379AlaArg: 3.379 ± 0.109
4.02AlaSer: 4.02 ± 0.113
4.911AlaThr: 4.911 ± 0.181
5.383AlaVal: 5.383 ± 0.112
0.86AlaTrp: 0.86 ± 0.045
2.477AlaTyr: 2.477 ± 0.076
0.0AlaXaa: 0.0 ± 0.0
Cys
0.771CysAla: 0.771 ± 0.042
0.169CysCys: 0.169 ± 0.019
0.676CysAsp: 0.676 ± 0.032
0.644CysGlu: 0.644 ± 0.036
0.499CysPhe: 0.499 ± 0.034
0.947CysGly: 0.947 ± 0.046
0.384CysHis: 0.384 ± 0.037
0.657CysIle: 0.657 ± 0.041
0.47CysLys: 0.47 ± 0.031
0.991CysLeu: 0.991 ± 0.047
0.154CysMet: 0.154 ± 0.021
0.305CysAsn: 0.305 ± 0.023
0.423CysPro: 0.423 ± 0.028
0.496CysGln: 0.496 ± 0.035
0.408CysArg: 0.408 ± 0.033
0.598CysSer: 0.598 ± 0.037
0.525CysThr: 0.525 ± 0.029
0.706CysVal: 0.706 ± 0.037
0.115CysTrp: 0.115 ± 0.013
0.379CysTyr: 0.379 ± 0.03
0.0CysXaa: 0.0 ± 0.0
Asp
4.092AspAla: 4.092 ± 0.105
0.576AspCys: 0.576 ± 0.036
2.592AspAsp: 2.592 ± 0.084
3.648AspGlu: 3.648 ± 0.1
2.677AspPhe: 2.677 ± 0.076
3.279AspGly: 3.279 ± 0.145
1.064AspHis: 1.064 ± 0.049
4.064AspIle: 4.064 ± 0.089
3.758AspLys: 3.758 ± 0.113
5.256AspLeu: 5.256 ± 0.112
1.292AspMet: 1.292 ± 0.053
2.794AspAsn: 2.794 ± 0.12
2.169AspPro: 2.169 ± 0.067
1.695AspGln: 1.695 ± 0.058
2.119AspArg: 2.119 ± 0.071
2.546AspSer: 2.546 ± 0.09
2.577AspThr: 2.577 ± 0.068
3.602AspVal: 3.602 ± 0.087
0.644AspTrp: 0.644 ± 0.036
2.089AspTyr: 2.089 ± 0.069
0.0AspXaa: 0.0 ± 0.0
Glu
4.324GluAla: 4.324 ± 0.113
0.466GluCys: 0.466 ± 0.033
2.909GluAsp: 2.909 ± 0.1
3.936GluGlu: 3.936 ± 0.141
2.158GluPhe: 2.158 ± 0.064
3.19GluGly: 3.19 ± 0.088
1.229GluHis: 1.229 ± 0.053
4.426GluIle: 4.426 ± 0.107
5.195GluLys: 5.195 ± 0.124
5.726GluLeu: 5.726 ± 0.153
1.833GluMet: 1.833 ± 0.059
4.074GluAsn: 4.074 ± 0.127
1.541GluPro: 1.541 ± 0.06
3.008GluGln: 3.008 ± 0.085
2.974GluArg: 2.974 ± 0.092
2.813GluSer: 2.813 ± 0.095
3.006GluThr: 3.006 ± 0.088
3.706GluVal: 3.706 ± 0.097
0.628GluTrp: 0.628 ± 0.039
1.678GluTyr: 1.678 ± 0.064
0.0GluXaa: 0.0 ± 0.0
Phe
3.559PheAla: 3.559 ± 0.106
0.665PheCys: 0.665 ± 0.04
2.494PheAsp: 2.494 ± 0.088
2.128PheGlu: 2.128 ± 0.069
2.187PhePhe: 2.187 ± 0.09
3.227PheGly: 3.227 ± 0.091
0.878PheHis: 0.878 ± 0.04
3.494PheIle: 3.494 ± 0.111
2.319PheLys: 2.319 ± 0.068
4.231PheLeu: 4.231 ± 0.143
1.175PheMet: 1.175 ± 0.054
2.265PheAsn: 2.265 ± 0.069
1.573PhePro: 1.573 ± 0.066
1.526PheGln: 1.526 ± 0.059
1.489PheArg: 1.489 ± 0.058
3.028PheSer: 3.028 ± 0.086
2.445PheThr: 2.445 ± 0.071
2.787PheVal: 2.787 ± 0.079
0.531PheTrp: 0.531 ± 0.034
1.68PheTyr: 1.68 ± 0.058
0.0PheXaa: 0.0 ± 0.0
Gly
5.004GlyAla: 5.004 ± 0.134
0.81GlyCys: 0.81 ± 0.042
3.622GlyAsp: 3.622 ± 0.168
4.217GlyGlu: 4.217 ± 0.12
3.106GlyPhe: 3.106 ± 0.073
4.423GlyGly: 4.423 ± 0.128
1.428GlyHis: 1.428 ± 0.054
5.377GlyIle: 5.377 ± 0.122
5.05GlyLys: 5.05 ± 0.149
6.664GlyLeu: 6.664 ± 0.14
1.768GlyMet: 1.768 ± 0.062
3.134GlyAsn: 3.134 ± 0.184
1.419GlyPro: 1.419 ± 0.185
2.592GlyGln: 2.592 ± 0.078
2.679GlyArg: 2.679 ± 0.082
3.481GlySer: 3.481 ± 0.13
3.671GlyThr: 3.671 ± 0.169
4.839GlyVal: 4.839 ± 0.099
0.858GlyTrp: 0.858 ± 0.043
2.358GlyTyr: 2.358 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
1.601HisAla: 1.601 ± 0.051
0.364HisCys: 0.364 ± 0.027
0.991HisAsp: 0.991 ± 0.043
1.001HisGlu: 1.001 ± 0.045
1.266HisPhe: 1.266 ± 0.048
1.426HisGly: 1.426 ± 0.058
0.754HisHis: 0.754 ± 0.042
1.623HisIle: 1.623 ± 0.056
1.129HisLys: 1.129 ± 0.043
2.445HisLeu: 2.445 ± 0.072
0.433HisMet: 0.433 ± 0.029
0.98HisAsn: 0.98 ± 0.045
1.116HisPro: 1.116 ± 0.048
1.186HisGln: 1.186 ± 0.05
0.953HisArg: 0.953 ± 0.043
1.305HisSer: 1.305 ± 0.048
1.175HisThr: 1.175 ± 0.049
1.177HisVal: 1.177 ± 0.05
0.325HisTrp: 0.325 ± 0.026
0.98HisTyr: 0.98 ± 0.044
0.0HisXaa: 0.0 ± 0.0
Ile
6.874IleAla: 6.874 ± 0.147
0.817IleCys: 0.817 ± 0.047
3.946IleAsp: 3.946 ± 0.087
4.204IleGlu: 4.204 ± 0.112
3.281IlePhe: 3.281 ± 0.101
5.366IleGly: 5.366 ± 0.192
1.523IleHis: 1.523 ± 0.059
5.121IleIle: 5.121 ± 0.138
4.131IleLys: 4.131 ± 0.098
7.095IleLeu: 7.095 ± 0.175
1.433IleMet: 1.433 ± 0.054
3.474IleAsn: 3.474 ± 0.106
3.01IlePro: 3.01 ± 0.083
2.895IleGln: 2.895 ± 0.073
2.761IleArg: 2.761 ± 0.086
4.506IleSer: 4.506 ± 0.108
4.204IleThr: 4.204 ± 0.112
4.53IleVal: 4.53 ± 0.114
0.672IleTrp: 0.672 ± 0.042
2.057IleTyr: 2.057 ± 0.075
0.0IleXaa: 0.0 ± 0.0
Lys
5.886LysAla: 5.886 ± 0.19
0.449LysCys: 0.449 ± 0.031
3.661LysAsp: 3.661 ± 0.104
4.087LysGlu: 4.087 ± 0.125
2.063LysPhe: 2.063 ± 0.071
3.946LysGly: 3.946 ± 0.11
1.257LysHis: 1.257 ± 0.042
4.547LysIle: 4.547 ± 0.114
4.438LysLys: 4.438 ± 0.128
5.923LysLeu: 5.923 ± 0.117
2.007LysMet: 2.007 ± 0.065
3.936LysAsn: 3.936 ± 0.11
2.43LysPro: 2.43 ± 0.081
3.117LysGln: 3.117 ± 0.092
2.748LysArg: 2.748 ± 0.087
3.34LysSer: 3.34 ± 0.105
3.871LysThr: 3.871 ± 0.129
4.22LysVal: 4.22 ± 0.114
0.676LysTrp: 0.676 ± 0.036
1.743LysTyr: 1.743 ± 0.06
0.0LysXaa: 0.0 ± 0.0
Leu
9.248LeuAla: 9.248 ± 0.168
1.125LeuCys: 1.125 ± 0.05
5.756LeuAsp: 5.756 ± 0.115
5.546LeuGlu: 5.546 ± 0.135
4.471LeuPhe: 4.471 ± 0.142
7.091LeuGly: 7.091 ± 0.139
2.221LeuHis: 2.221 ± 0.071
6.604LeuIle: 6.604 ± 0.169
6.088LeuLys: 6.088 ± 0.116
10.097LeuLeu: 10.097 ± 0.221
2.547LeuMet: 2.547 ± 0.08
5.119LeuAsn: 5.119 ± 0.115
4.792LeuPro: 4.792 ± 0.123
4.499LeuGln: 4.499 ± 0.134
4.549LeuArg: 4.549 ± 0.122
6.853LeuSer: 6.853 ± 0.137
6.042LeuThr: 6.042 ± 0.116
6.021LeuVal: 6.021 ± 0.125
1.082LeuTrp: 1.082 ± 0.048
2.789LeuTyr: 2.789 ± 0.09
0.0LeuXaa: 0.0 ± 0.0
Met
2.338MetAla: 2.338 ± 0.076
0.191MetCys: 0.191 ± 0.017
1.062MetAsp: 1.062 ± 0.047
1.199MetGlu: 1.199 ± 0.056
0.83MetPhe: 0.83 ± 0.045
1.717MetGly: 1.717 ± 0.06
0.551MetHis: 0.551 ± 0.033
1.552MetIle: 1.552 ± 0.062
1.606MetLys: 1.606 ± 0.062
2.731MetLeu: 2.731 ± 0.095
0.655MetMet: 0.655 ± 0.042
1.181MetAsn: 1.181 ± 0.048
1.212MetPro: 1.212 ± 0.058
1.541MetGln: 1.541 ± 0.058
1.168MetArg: 1.168 ± 0.048
1.484MetSer: 1.484 ± 0.053
1.513MetThr: 1.513 ± 0.052
1.575MetVal: 1.575 ± 0.063
0.232MetTrp: 0.232 ± 0.023
0.568MetTyr: 0.568 ± 0.031
0.0MetXaa: 0.0 ± 0.0
Asn
4.165AsnAla: 4.165 ± 0.142
0.399AsnCys: 0.399 ± 0.03
2.427AsnAsp: 2.427 ± 0.088
2.466AsnGlu: 2.466 ± 0.081
2.126AsnPhe: 2.126 ± 0.064
3.695AsnGly: 3.695 ± 0.234
1.066AsnHis: 1.066 ± 0.045
3.853AsnIle: 3.853 ± 0.127
3.409AsnLys: 3.409 ± 0.177
5.045AsnLeu: 5.045 ± 0.113
1.162AsnMet: 1.162 ± 0.044
2.525AsnAsn: 2.525 ± 0.114
2.568AsnPro: 2.568 ± 0.069
2.416AsnGln: 2.416 ± 0.082
2.003AsnArg: 2.003 ± 0.065
2.559AsnSer: 2.559 ± 0.094
2.928AsnThr: 2.928 ± 0.171
3.305AsnVal: 3.305 ± 0.135
0.587AsnTrp: 0.587 ± 0.037
1.613AsnTyr: 1.613 ± 0.058
0.0AsnXaa: 0.0 ± 0.0
Pro
3.075ProAla: 3.075 ± 0.107
0.318ProCys: 0.318 ± 0.025
2.015ProAsp: 2.015 ± 0.066
2.815ProGlu: 2.815 ± 0.09
1.875ProPhe: 1.875 ± 0.068
1.63ProGly: 1.63 ± 0.068
1.043ProHis: 1.043 ± 0.041
2.709ProIle: 2.709 ± 0.073
2.304ProLys: 2.304 ± 0.074
3.977ProLeu: 3.977 ± 0.092
1.01ProMet: 1.01 ± 0.051
2.178ProAsn: 2.178 ± 0.077
1.199ProPro: 1.199 ± 0.054
2.107ProGln: 2.107 ± 0.068
1.363ProArg: 1.363 ± 0.055
2.059ProSer: 2.059 ± 0.059
2.475ProThr: 2.475 ± 0.076
2.83ProVal: 2.83 ± 0.076
0.399ProTrp: 0.399 ± 0.028
1.539ProTyr: 1.539 ± 0.057
0.0ProXaa: 0.0 ± 0.0
Gln
4.287GlnAla: 4.287 ± 0.111
0.438GlnCys: 0.438 ± 0.028
2.302GlnAsp: 2.302 ± 0.073
2.648GlnGlu: 2.648 ± 0.078
1.864GlnPhe: 1.864 ± 0.067
3.069GlnGly: 3.069 ± 0.125
1.125GlnHis: 1.125 ± 0.048
3.188GlnIle: 3.188 ± 0.089
3.294GlnLys: 3.294 ± 0.084
4.776GlnLeu: 4.776 ± 0.112
1.133GlnMet: 1.133 ± 0.049
2.494GlnAsn: 2.494 ± 0.102
1.656GlnPro: 1.656 ± 0.062
3.218GlnGln: 3.218 ± 0.104
2.167GlnArg: 2.167 ± 0.077
2.611GlnSer: 2.611 ± 0.072
2.377GlnThr: 2.377 ± 0.082
2.895GlnVal: 2.895 ± 0.082
0.544GlnTrp: 0.544 ± 0.035
1.487GlnTyr: 1.487 ± 0.053
0.0GlnXaa: 0.0 ± 0.0
Arg
3.108ArgAla: 3.108 ± 0.085
0.371ArgCys: 0.371 ± 0.027
2.098ArgAsp: 2.098 ± 0.068
2.689ArgGlu: 2.689 ± 0.096
2.087ArgPhe: 2.087 ± 0.063
2.521ArgGly: 2.521 ± 0.095
1.077ArgHis: 1.077 ± 0.046
3.244ArgIle: 3.244 ± 0.082
2.547ArgLys: 2.547 ± 0.082
4.579ArgLeu: 4.579 ± 0.136
1.019ArgMet: 1.019 ± 0.047
1.976ArgAsn: 1.976 ± 0.066
1.625ArgPro: 1.625 ± 0.068
2.236ArgGln: 2.236 ± 0.076
2.074ArgArg: 2.074 ± 0.079
2.013ArgSer: 2.013 ± 0.073
2.063ArgThr: 2.063 ± 0.068
2.616ArgVal: 2.616 ± 0.092
0.47ArgTrp: 0.47 ± 0.029
1.714ArgTyr: 1.714 ± 0.068
0.0ArgXaa: 0.0 ± 0.0
Ser
4.653SerAla: 4.653 ± 0.188
0.512SerCys: 0.512 ± 0.034
2.934SerAsp: 2.934 ± 0.07
3.03SerGlu: 3.03 ± 0.075
2.553SerPhe: 2.553 ± 0.076
4.116SerGly: 4.116 ± 0.14
1.446SerHis: 1.446 ± 0.061
3.931SerIle: 3.931 ± 0.114
2.999SerLys: 2.999 ± 0.074
5.908SerLeu: 5.908 ± 0.116
1.342SerMet: 1.342 ± 0.059
2.354SerAsn: 2.354 ± 0.092
2.236SerPro: 2.236 ± 0.071
2.728SerGln: 2.728 ± 0.068
2.323SerArg: 2.323 ± 0.085
3.156SerSer: 3.156 ± 0.093
3.119SerThr: 3.119 ± 0.091
3.559SerVal: 3.559 ± 0.101
0.629SerTrp: 0.629 ± 0.038
1.87SerTyr: 1.87 ± 0.065
0.0SerXaa: 0.0 ± 0.0
Thr
4.939ThrAla: 4.939 ± 0.184
0.511ThrCys: 0.511 ± 0.034
2.909ThrAsp: 2.909 ± 0.105
3.017ThrGlu: 3.017 ± 0.082
2.533ThrPhe: 2.533 ± 0.073
4.085ThrGly: 4.085 ± 0.144
1.283ThrHis: 1.283 ± 0.053
3.795ThrIle: 3.795 ± 0.114
2.974ThrLys: 2.974 ± 0.091
6.428ThrLeu: 6.428 ± 0.137
1.107ThrMet: 1.107 ± 0.044
2.488ThrAsn: 2.488 ± 0.135
2.791ThrPro: 2.791 ± 0.069
2.9ThrGln: 2.9 ± 0.098
2.052ThrArg: 2.052 ± 0.071
2.963ThrSer: 2.963 ± 0.095
3.548ThrThr: 3.548 ± 0.193
3.903ThrVal: 3.903 ± 0.153
0.509ThrTrp: 0.509 ± 0.034
1.628ThrTyr: 1.628 ± 0.063
0.0ThrXaa: 0.0 ± 0.0
Val
5.94ValAla: 5.94 ± 0.139
0.657ValCys: 0.657 ± 0.039
3.587ValAsp: 3.587 ± 0.098
4.155ValGlu: 4.155 ± 0.117
2.412ValPhe: 2.412 ± 0.081
4.48ValGly: 4.48 ± 0.116
1.181ValHis: 1.181 ± 0.044
4.681ValIle: 4.681 ± 0.11
4.328ValLys: 4.328 ± 0.098
6.315ValLeu: 6.315 ± 0.139
1.61ValMet: 1.61 ± 0.069
3.303ValAsn: 3.303 ± 0.164
2.612ValPro: 2.612 ± 0.077
2.284ValGln: 2.284 ± 0.071
2.633ValArg: 2.633 ± 0.095
3.855ValSer: 3.855 ± 0.105
3.962ValThr: 3.962 ± 0.139
4.592ValVal: 4.592 ± 0.113
0.6ValTrp: 0.6 ± 0.035
1.719ValTyr: 1.719 ± 0.063
0.0ValXaa: 0.0 ± 0.0
Trp
0.72TrpAla: 0.72 ± 0.039
0.156TrpCys: 0.156 ± 0.017
0.527TrpAsp: 0.527 ± 0.034
0.525TrpGlu: 0.525 ± 0.03
0.535TrpPhe: 0.535 ± 0.035
0.685TrpGly: 0.685 ± 0.04
0.312TrpHis: 0.312 ± 0.023
0.722TrpIle: 0.722 ± 0.04
0.629TrpLys: 0.629 ± 0.038
1.439TrpLeu: 1.439 ± 0.055
0.245TrpMet: 0.245 ± 0.02
0.477TrpAsn: 0.477 ± 0.031
0.178TrpPro: 0.178 ± 0.021
1.029TrpGln: 1.029 ± 0.051
0.57TrpArg: 0.57 ± 0.035
0.462TrpSer: 0.462 ± 0.032
0.499TrpThr: 0.499 ± 0.032
0.704TrpVal: 0.704 ± 0.042
0.188TrpTrp: 0.188 ± 0.021
0.395TrpTyr: 0.395 ± 0.032
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.536TyrAla: 2.536 ± 0.072
0.425TyrCys: 0.425 ± 0.029
1.665TyrAsp: 1.665 ± 0.061
1.545TyrGlu: 1.545 ± 0.063
1.805TyrPhe: 1.805 ± 0.07
2.115TyrGly: 2.115 ± 0.073
0.901TyrHis: 0.901 ± 0.04
1.925TyrIle: 1.925 ± 0.066
1.632TyrLys: 1.632 ± 0.069
3.622TyrLeu: 3.622 ± 0.095
0.576TyrMet: 0.576 ± 0.037
1.352TyrAsn: 1.352 ± 0.068
1.504TyrPro: 1.504 ± 0.065
1.844TyrGln: 1.844 ± 0.066
1.714TyrArg: 1.714 ± 0.058
1.747TyrSer: 1.747 ± 0.06
1.45TyrThr: 1.45 ± 0.05
1.957TyrVal: 1.957 ± 0.062
0.451TyrTrp: 0.451 ± 0.034
1.177TyrTyr: 1.177 ± 0.055
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1624 proteins (538582 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski