Pelargonium vein banding virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Caulimoviridae; Badnavirus; unclassified Badnavirus

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|C9DT06|C9DT06_9VIRU Uncharacterized protein OS=Pelargonium vein banding virus OX=671126 PE=4 SV=1
MM1 pKa = 7.75SEE3 pKa = 4.29RR4 pKa = 11.84FEE6 pKa = 3.93QAIQKK11 pKa = 8.73WYY13 pKa = 10.45NEE15 pKa = 3.75SRR17 pKa = 11.84TADD20 pKa = 3.75LRR22 pKa = 11.84YY23 pKa = 10.0LDD25 pKa = 5.15LATEE29 pKa = 4.36SPTVKK34 pKa = 10.31DD35 pKa = 3.58YY36 pKa = 11.79CSVINNNVSVIYY48 pKa = 10.4DD49 pKa = 3.53RR50 pKa = 11.84LLLHH54 pKa = 6.55SKK56 pKa = 10.18VAIKK60 pKa = 9.28DD61 pKa = 3.37TYY63 pKa = 10.83KK64 pKa = 10.53ILEE67 pKa = 4.32AQEE70 pKa = 4.18ANQDD74 pKa = 3.3ILVQEE79 pKa = 4.21IRR81 pKa = 11.84RR82 pKa = 11.84LSKK85 pKa = 10.2RR86 pKa = 11.84VKK88 pKa = 10.27ALEE91 pKa = 4.15AEE93 pKa = 4.25ALASKK98 pKa = 10.18PLTAEE103 pKa = 3.89QVRR106 pKa = 11.84EE107 pKa = 4.03LVKK110 pKa = 10.82EE111 pKa = 4.06IAAQPKK117 pKa = 9.83LVEE120 pKa = 4.04EE121 pKa = 4.28QAFKK125 pKa = 10.2LTTDD129 pKa = 3.54LRR131 pKa = 11.84SQVLQVKK138 pKa = 9.31EE139 pKa = 3.96LVEE142 pKa = 4.33KK143 pKa = 10.32VQHH146 pKa = 6.26LLVSS150 pKa = 4.15

Molecular weight:
17.27 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|C9DT07|C9DT07_9VIRU Reverse transcriptase OS=Pelargonium vein banding virus OX=671126 PE=4 SV=1
MM1 pKa = 7.46SRR3 pKa = 11.84PDD5 pKa = 3.23SATTAEE11 pKa = 4.57YY12 pKa = 10.16IAAAQATEE20 pKa = 4.11ALGSPAEE27 pKa = 4.41GFLSVKK33 pKa = 10.23NIPPGSSSQTALLIKK48 pKa = 10.35QNNFLLEE55 pKa = 4.18LVLDD59 pKa = 3.85LHH61 pKa = 6.9RR62 pKa = 11.84KK63 pKa = 8.29INGVEE68 pKa = 3.85ARR70 pKa = 11.84LSRR73 pKa = 11.84AKK75 pKa = 10.43EE76 pKa = 3.74PAEE79 pKa = 4.24GSQGQGLEE87 pKa = 4.22EE88 pKa = 5.03IIQKK92 pKa = 10.1LDD94 pKa = 3.13KK95 pKa = 11.16LKK97 pKa = 10.83IGDD100 pKa = 4.3GEE102 pKa = 4.42QKK104 pKa = 8.24THH106 pKa = 6.6SIKK109 pKa = 11.0GPGKK113 pKa = 9.13VYY115 pKa = 9.99FYY117 pKa = 10.82RR118 pKa = 11.84DD119 pKa = 3.51PKK121 pKa = 11.05ALAEE125 pKa = 4.05EE126 pKa = 4.37EE127 pKa = 3.95RR128 pKa = 11.84RR129 pKa = 11.84KK130 pKa = 10.73AKK132 pKa = 10.12LRR134 pKa = 11.84YY135 pKa = 9.09AA136 pKa = 3.92

Molecular weight:
14.86 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2256

136

1970

752.0

85.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.979 ± 1.301

1.463 ± 0.558

5.053 ± 0.454

8.333 ± 0.88

2.97 ± 0.597

5.541 ± 1.422

1.995 ± 0.288

6.161 ± 0.281

6.693 ± 1.182

8.732 ± 1.71

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.261 ± 0.757

4.167 ± 0.503

5.674 ± 1.125

4.787 ± 0.779

6.738 ± 0.387

6.25 ± 0.383

4.965 ± 0.413

5.496 ± 1.167

1.418 ± 0.536

3.324 ± 0.109

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski