Amino acid dipepetide frequency for Escherichia phage vB_EcoS_CEB_EC3a

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.249AlaAla: 8.249 ± 1.103
0.825AlaCys: 0.825 ± 0.263
3.975AlaAsp: 3.975 ± 0.525
5.249AlaGlu: 5.249 ± 0.588
3.225AlaPhe: 3.225 ± 0.439
6.299AlaGly: 6.299 ± 0.631
1.2AlaHis: 1.2 ± 0.326
5.774AlaIle: 5.774 ± 0.72
6.074AlaLys: 6.074 ± 1.051
7.049AlaLeu: 7.049 ± 0.991
2.325AlaMet: 2.325 ± 0.512
4.649AlaAsn: 4.649 ± 0.672
2.1AlaPro: 2.1 ± 0.367
3.15AlaGln: 3.15 ± 0.549
4.724AlaArg: 4.724 ± 0.622
5.624AlaSer: 5.624 ± 0.688
5.099AlaThr: 5.099 ± 0.552
5.699AlaVal: 5.699 ± 0.738
1.05AlaTrp: 1.05 ± 0.272
2.475AlaTyr: 2.475 ± 0.324
0.0AlaXaa: 0.0 ± 0.0
Cys
0.75CysAla: 0.75 ± 0.334
0.3CysCys: 0.3 ± 0.149
0.675CysAsp: 0.675 ± 0.195
0.825CysGlu: 0.825 ± 0.26
0.3CysPhe: 0.3 ± 0.138
1.125CysGly: 1.125 ± 0.293
0.3CysHis: 0.3 ± 0.131
0.375CysIle: 0.375 ± 0.188
1.35CysLys: 1.35 ± 0.326
1.05CysLeu: 1.05 ± 0.268
0.45CysMet: 0.45 ± 0.194
0.525CysAsn: 0.525 ± 0.191
0.3CysPro: 0.3 ± 0.134
0.15CysGln: 0.15 ± 0.112
0.675CysArg: 0.675 ± 0.27
1.05CysSer: 1.05 ± 0.323
0.675CysThr: 0.675 ± 0.26
0.75CysVal: 0.75 ± 0.227
0.375CysTrp: 0.375 ± 0.151
0.225CysTyr: 0.225 ± 0.127
0.0CysXaa: 0.0 ± 0.0
Asp
4.574AspAla: 4.574 ± 0.621
0.525AspCys: 0.525 ± 0.241
3.225AspAsp: 3.225 ± 0.53
4.199AspGlu: 4.199 ± 0.611
2.775AspPhe: 2.775 ± 0.473
7.124AspGly: 7.124 ± 0.799
0.825AspHis: 0.825 ± 0.3
4.124AspIle: 4.124 ± 0.542
4.649AspLys: 4.649 ± 0.592
4.049AspLeu: 4.049 ± 0.62
1.8AspMet: 1.8 ± 0.394
2.925AspAsn: 2.925 ± 0.473
1.425AspPro: 1.425 ± 0.335
1.05AspGln: 1.05 ± 0.234
1.725AspArg: 1.725 ± 0.387
3.225AspSer: 3.225 ± 0.581
3.75AspThr: 3.75 ± 0.566
4.049AspVal: 4.049 ± 0.505
1.35AspTrp: 1.35 ± 0.251
3.075AspTyr: 3.075 ± 0.434
0.0AspXaa: 0.0 ± 0.0
Glu
5.474GluAla: 5.474 ± 0.55
0.75GluCys: 0.75 ± 0.257
3.075GluAsp: 3.075 ± 0.376
4.724GluGlu: 4.724 ± 0.812
3.6GluPhe: 3.6 ± 0.546
3.675GluGly: 3.675 ± 0.569
0.6GluHis: 0.6 ± 0.204
5.624GluIle: 5.624 ± 0.588
4.274GluLys: 4.274 ± 0.605
5.999GluLeu: 5.999 ± 0.763
2.85GluMet: 2.85 ± 0.641
3.075GluAsn: 3.075 ± 0.451
2.025GluPro: 2.025 ± 0.343
3.075GluGln: 3.075 ± 0.72
2.7GluArg: 2.7 ± 0.477
4.349GluSer: 4.349 ± 0.533
2.925GluThr: 2.925 ± 0.387
5.474GluVal: 5.474 ± 0.764
0.825GluTrp: 0.825 ± 0.235
2.475GluTyr: 2.475 ± 0.398
0.0GluXaa: 0.0 ± 0.0
Phe
2.55PheAla: 2.55 ± 0.388
0.825PheCys: 0.825 ± 0.324
3.45PheAsp: 3.45 ± 0.466
3.15PheGlu: 3.15 ± 0.594
1.05PhePhe: 1.05 ± 0.257
3.9PheGly: 3.9 ± 0.445
0.825PheHis: 0.825 ± 0.265
2.25PheIle: 2.25 ± 0.382
3.15PheLys: 3.15 ± 0.569
2.325PheLeu: 2.325 ± 0.461
0.9PheMet: 0.9 ± 0.234
1.725PheAsn: 1.725 ± 0.343
1.125PhePro: 1.125 ± 0.253
1.5PheGln: 1.5 ± 0.315
2.25PheArg: 2.25 ± 0.534
2.7PheSer: 2.7 ± 0.504
2.4PheThr: 2.4 ± 0.305
2.55PheVal: 2.55 ± 0.465
0.375PheTrp: 0.375 ± 0.127
1.2PheTyr: 1.2 ± 0.302
0.0PheXaa: 0.0 ± 0.0
Gly
5.174GlyAla: 5.174 ± 0.753
1.275GlyCys: 1.275 ± 0.3
4.049GlyAsp: 4.049 ± 0.516
4.874GlyGlu: 4.874 ± 0.637
3.0GlyPhe: 3.0 ± 0.456
6.224GlyGly: 6.224 ± 1.117
0.975GlyHis: 0.975 ± 0.316
5.849GlyIle: 5.849 ± 0.642
5.324GlyLys: 5.324 ± 0.75
7.199GlyLeu: 7.199 ± 0.683
2.025GlyMet: 2.025 ± 0.46
3.45GlyAsn: 3.45 ± 0.616
0.75GlyPro: 0.75 ± 0.24
2.25GlyGln: 2.25 ± 0.416
3.15GlyArg: 3.15 ± 0.433
5.174GlySer: 5.174 ± 0.631
3.525GlyThr: 3.525 ± 0.524
5.624GlyVal: 5.624 ± 0.63
1.275GlyTrp: 1.275 ± 0.359
4.274GlyTyr: 4.274 ± 0.64
0.0GlyXaa: 0.0 ± 0.0
His
1.125HisAla: 1.125 ± 0.295
0.3HisCys: 0.3 ± 0.158
0.825HisAsp: 0.825 ± 0.227
0.825HisGlu: 0.825 ± 0.23
0.525HisPhe: 0.525 ± 0.173
1.05HisGly: 1.05 ± 0.324
0.45HisHis: 0.45 ± 0.189
1.275HisIle: 1.275 ± 0.287
1.35HisLys: 1.35 ± 0.318
1.05HisLeu: 1.05 ± 0.247
0.375HisMet: 0.375 ± 0.179
0.675HisAsn: 0.675 ± 0.243
0.075HisPro: 0.075 ± 0.082
0.525HisGln: 0.525 ± 0.182
0.675HisArg: 0.675 ± 0.202
0.6HisSer: 0.6 ± 0.198
0.9HisThr: 0.9 ± 0.249
0.9HisVal: 0.9 ± 0.262
0.15HisTrp: 0.15 ± 0.11
0.75HisTyr: 0.75 ± 0.258
0.0HisXaa: 0.0 ± 0.0
Ile
6.149IleAla: 6.149 ± 0.875
0.45IleCys: 0.45 ± 0.17
5.774IleAsp: 5.774 ± 0.596
3.825IleGlu: 3.825 ± 0.499
1.95IlePhe: 1.95 ± 0.366
4.574IleGly: 4.574 ± 0.561
1.05IleHis: 1.05 ± 0.296
3.825IleIle: 3.825 ± 0.602
4.874IleLys: 4.874 ± 0.69
3.525IleLeu: 3.525 ± 0.619
1.575IleMet: 1.575 ± 0.427
4.049IleAsn: 4.049 ± 0.622
2.85IlePro: 2.85 ± 0.483
2.625IleGln: 2.625 ± 0.513
2.7IleArg: 2.7 ± 0.359
5.249IleSer: 5.249 ± 0.645
5.699IleThr: 5.699 ± 0.56
3.45IleVal: 3.45 ± 0.522
0.675IleTrp: 0.675 ± 0.196
2.7IleTyr: 2.7 ± 0.499
0.0IleXaa: 0.0 ± 0.0
Lys
6.824LysAla: 6.824 ± 0.947
0.6LysCys: 0.6 ± 0.238
4.349LysAsp: 4.349 ± 0.565
5.024LysGlu: 5.024 ± 0.902
3.225LysPhe: 3.225 ± 0.449
3.15LysGly: 3.15 ± 0.429
0.75LysHis: 0.75 ± 0.222
3.15LysIle: 3.15 ± 0.427
4.124LysLys: 4.124 ± 0.687
4.799LysLeu: 4.799 ± 0.837
3.0LysMet: 3.0 ± 0.545
3.15LysAsn: 3.15 ± 0.519
1.8LysPro: 1.8 ± 0.395
3.225LysGln: 3.225 ± 0.418
2.7LysArg: 2.7 ± 0.555
4.049LysSer: 4.049 ± 0.561
3.525LysThr: 3.525 ± 0.567
5.174LysVal: 5.174 ± 0.672
0.825LysTrp: 0.825 ± 0.275
3.15LysTyr: 3.15 ± 0.429
0.0LysXaa: 0.0 ± 0.0
Leu
5.924LeuAla: 5.924 ± 0.651
0.975LeuCys: 0.975 ± 0.244
4.574LeuAsp: 4.574 ± 0.432
3.675LeuGlu: 3.675 ± 0.619
1.875LeuPhe: 1.875 ± 0.302
4.274LeuGly: 4.274 ± 0.646
1.2LeuHis: 1.2 ± 0.36
6.149LeuIle: 6.149 ± 0.746
4.049LeuLys: 4.049 ± 0.52
4.574LeuLeu: 4.574 ± 0.595
0.825LeuMet: 0.825 ± 0.254
3.525LeuAsn: 3.525 ± 0.448
2.925LeuPro: 2.925 ± 0.476
2.55LeuGln: 2.55 ± 0.767
4.199LeuArg: 4.199 ± 0.595
6.824LeuSer: 6.824 ± 0.699
4.574LeuThr: 4.574 ± 0.704
5.849LeuVal: 5.849 ± 0.504
0.3LeuTrp: 0.3 ± 0.165
2.025LeuTyr: 2.025 ± 0.477
0.0LeuXaa: 0.0 ± 0.0
Met
3.15MetAla: 3.15 ± 0.477
0.225MetCys: 0.225 ± 0.142
1.05MetAsp: 1.05 ± 0.267
1.5MetGlu: 1.5 ± 0.318
1.35MetPhe: 1.35 ± 0.324
0.825MetGly: 0.825 ± 0.236
0.15MetHis: 0.15 ± 0.104
1.8MetIle: 1.8 ± 0.289
1.875MetLys: 1.875 ± 0.405
2.1MetLeu: 2.1 ± 0.484
0.825MetMet: 0.825 ± 0.3
1.5MetAsn: 1.5 ± 0.336
0.6MetPro: 0.6 ± 0.192
1.05MetGln: 1.05 ± 0.262
1.425MetArg: 1.425 ± 0.345
1.875MetSer: 1.875 ± 0.42
2.1MetThr: 2.1 ± 0.45
1.425MetVal: 1.425 ± 0.283
0.375MetTrp: 0.375 ± 0.176
0.375MetTyr: 0.375 ± 0.209
0.0MetXaa: 0.0 ± 0.0
Asn
4.424AsnAla: 4.424 ± 0.635
0.75AsnCys: 0.75 ± 0.224
3.0AsnAsp: 3.0 ± 0.332
4.349AsnGlu: 4.349 ± 0.67
2.325AsnPhe: 2.325 ± 0.419
5.924AsnGly: 5.924 ± 1.224
0.9AsnHis: 0.9 ± 0.295
2.85AsnIle: 2.85 ± 0.454
2.55AsnLys: 2.55 ± 0.406
3.375AsnLeu: 3.375 ± 0.71
0.9AsnMet: 0.9 ± 0.234
3.0AsnAsn: 3.0 ± 0.48
1.8AsnPro: 1.8 ± 0.348
2.175AsnGln: 2.175 ± 0.516
1.95AsnArg: 1.95 ± 0.328
3.525AsnSer: 3.525 ± 0.46
2.25AsnThr: 2.25 ± 0.5
3.75AsnVal: 3.75 ± 0.411
0.825AsnTrp: 0.825 ± 0.201
1.875AsnTyr: 1.875 ± 0.404
0.0AsnXaa: 0.0 ± 0.0
Pro
3.225ProAla: 3.225 ± 0.486
0.375ProCys: 0.375 ± 0.173
1.95ProAsp: 1.95 ± 0.433
3.15ProGlu: 3.15 ± 0.416
1.65ProPhe: 1.65 ± 0.381
2.1ProGly: 2.1 ± 0.406
0.375ProHis: 0.375 ± 0.164
1.5ProIle: 1.5 ± 0.234
1.35ProLys: 1.35 ± 0.301
1.8ProLeu: 1.8 ± 0.428
0.375ProMet: 0.375 ± 0.188
1.35ProAsn: 1.35 ± 0.271
0.525ProPro: 0.525 ± 0.201
1.2ProGln: 1.2 ± 0.315
1.5ProArg: 1.5 ± 0.309
1.05ProSer: 1.05 ± 0.27
1.95ProThr: 1.95 ± 0.42
2.85ProVal: 2.85 ± 0.568
0.375ProTrp: 0.375 ± 0.185
1.425ProTyr: 1.425 ± 0.283
0.0ProXaa: 0.0 ± 0.0
Gln
3.975GlnAla: 3.975 ± 0.927
0.3GlnCys: 0.3 ± 0.21
1.275GlnAsp: 1.275 ± 0.28
2.775GlnGlu: 2.775 ± 0.449
1.2GlnPhe: 1.2 ± 0.278
2.475GlnGly: 2.475 ± 0.395
0.3GlnHis: 0.3 ± 0.143
3.525GlnIle: 3.525 ± 0.669
2.025GlnLys: 2.025 ± 0.409
3.0GlnLeu: 3.0 ± 0.527
0.975GlnMet: 0.975 ± 0.326
1.575GlnAsn: 1.575 ± 0.344
1.35GlnPro: 1.35 ± 0.34
2.7GlnGln: 2.7 ± 0.839
1.725GlnArg: 1.725 ± 0.377
2.925GlnSer: 2.925 ± 0.505
1.725GlnThr: 1.725 ± 0.32
2.175GlnVal: 2.175 ± 0.362
0.45GlnTrp: 0.45 ± 0.17
1.275GlnTyr: 1.275 ± 0.262
0.0GlnXaa: 0.0 ± 0.0
Arg
3.9ArgAla: 3.9 ± 0.5
0.75ArgCys: 0.75 ± 0.332
2.175ArgAsp: 2.175 ± 0.384
3.15ArgGlu: 3.15 ± 0.51
2.475ArgPhe: 2.475 ± 0.333
2.55ArgGly: 2.55 ± 0.412
0.825ArgHis: 0.825 ± 0.259
3.825ArgIle: 3.825 ± 0.554
4.274ArgLys: 4.274 ± 0.593
3.3ArgLeu: 3.3 ± 0.511
1.125ArgMet: 1.125 ± 0.327
2.475ArgAsn: 2.475 ± 0.452
1.65ArgPro: 1.65 ± 0.422
1.35ArgGln: 1.35 ± 0.37
2.475ArgArg: 2.475 ± 0.337
2.475ArgSer: 2.475 ± 0.348
1.8ArgThr: 1.8 ± 0.469
3.45ArgVal: 3.45 ± 0.435
0.375ArgTrp: 0.375 ± 0.144
1.95ArgTyr: 1.95 ± 0.412
0.0ArgXaa: 0.0 ± 0.0
Ser
5.474SerAla: 5.474 ± 0.647
0.45SerCys: 0.45 ± 0.167
5.399SerAsp: 5.399 ± 0.509
5.024SerGlu: 5.024 ± 0.496
2.625SerPhe: 2.625 ± 0.422
6.149SerGly: 6.149 ± 0.69
1.275SerHis: 1.275 ± 0.237
4.274SerIle: 4.274 ± 0.416
3.375SerLys: 3.375 ± 0.534
4.799SerLeu: 4.799 ± 0.621
1.275SerMet: 1.275 ± 0.265
3.825SerAsn: 3.825 ± 0.672
2.25SerPro: 2.25 ± 0.385
2.175SerGln: 2.175 ± 0.386
2.85SerArg: 2.85 ± 0.426
4.499SerSer: 4.499 ± 0.715
4.274SerThr: 4.274 ± 0.533
5.099SerVal: 5.099 ± 0.524
0.675SerTrp: 0.675 ± 0.28
2.025SerTyr: 2.025 ± 0.405
0.0SerXaa: 0.0 ± 0.0
Thr
4.724ThrAla: 4.724 ± 0.592
0.675ThrCys: 0.675 ± 0.21
2.85ThrAsp: 2.85 ± 0.462
3.375ThrGlu: 3.375 ± 0.422
2.1ThrPhe: 2.1 ± 0.412
5.849ThrGly: 5.849 ± 0.704
0.6ThrHis: 0.6 ± 0.182
4.049ThrIle: 4.049 ± 0.447
3.3ThrLys: 3.3 ± 0.536
3.75ThrLeu: 3.75 ± 0.552
1.5ThrMet: 1.5 ± 0.325
3.525ThrAsn: 3.525 ± 0.565
2.475ThrPro: 2.475 ± 0.404
2.25ThrGln: 2.25 ± 0.353
2.1ThrArg: 2.1 ± 0.342
3.75ThrSer: 3.75 ± 0.484
2.925ThrThr: 2.925 ± 0.536
4.199ThrVal: 4.199 ± 0.498
0.525ThrTrp: 0.525 ± 0.199
3.075ThrTyr: 3.075 ± 0.455
0.0ThrXaa: 0.0 ± 0.0
Val
5.849ValAla: 5.849 ± 0.642
0.9ValCys: 0.9 ± 0.274
4.799ValAsp: 4.799 ± 0.539
4.724ValGlu: 4.724 ± 0.627
2.775ValPhe: 2.775 ± 0.368
4.349ValGly: 4.349 ± 0.554
0.9ValHis: 0.9 ± 0.203
4.199ValIle: 4.199 ± 0.508
5.249ValLys: 5.249 ± 0.682
3.825ValLeu: 3.825 ± 0.574
1.575ValMet: 1.575 ± 0.34
4.649ValAsn: 4.649 ± 0.481
2.1ValPro: 2.1 ± 0.375
2.775ValGln: 2.775 ± 0.639
3.975ValArg: 3.975 ± 0.586
5.249ValSer: 5.249 ± 0.567
4.424ValThr: 4.424 ± 0.546
4.724ValVal: 4.724 ± 0.656
0.9ValTrp: 0.9 ± 0.224
2.925ValTyr: 2.925 ± 0.452
0.0ValXaa: 0.0 ± 0.0
Trp
0.675TrpAla: 0.675 ± 0.192
0.3TrpCys: 0.3 ± 0.152
1.125TrpAsp: 1.125 ± 0.275
0.525TrpGlu: 0.525 ± 0.206
0.9TrpPhe: 0.9 ± 0.265
0.9TrpGly: 0.9 ± 0.234
0.3TrpHis: 0.3 ± 0.126
0.75TrpIle: 0.75 ± 0.228
1.05TrpLys: 1.05 ± 0.267
0.75TrpLeu: 0.75 ± 0.208
0.225TrpMet: 0.225 ± 0.126
0.45TrpAsn: 0.45 ± 0.138
0.45TrpPro: 0.45 ± 0.174
0.375TrpGln: 0.375 ± 0.138
0.75TrpArg: 0.75 ± 0.248
0.825TrpSer: 0.825 ± 0.322
0.525TrpThr: 0.525 ± 0.203
1.05TrpVal: 1.05 ± 0.27
0.075TrpTrp: 0.075 ± 0.08
0.225TrpTyr: 0.225 ± 0.118
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.625TyrAla: 2.625 ± 0.504
0.675TyrCys: 0.675 ± 0.216
2.85TyrAsp: 2.85 ± 0.577
2.625TyrGlu: 2.625 ± 0.467
1.275TyrPhe: 1.275 ± 0.325
2.925TyrGly: 2.925 ± 0.48
0.6TyrHis: 0.6 ± 0.224
2.4TyrIle: 2.4 ± 0.408
2.325TyrLys: 2.325 ± 0.494
2.55TyrLeu: 2.55 ± 0.382
0.6TyrMet: 0.6 ± 0.224
2.4TyrAsn: 2.4 ± 0.397
1.5TyrPro: 1.5 ± 0.351
1.575TyrGln: 1.575 ± 0.377
2.1TyrArg: 2.1 ± 0.342
2.85TyrSer: 2.85 ± 0.407
2.55TyrThr: 2.55 ± 0.437
2.625TyrVal: 2.625 ± 0.374
0.45TyrTrp: 0.45 ± 0.148
1.725TyrTyr: 1.725 ± 0.331
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 70 proteins (13336 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski