Amino acid dipepetide frequency for Microbacterium phage Krampus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.934AlaAla: 14.934 ± 1.808
0.389AlaCys: 0.389 ± 0.135
6.163AlaAsp: 6.163 ± 0.624
8.661AlaGlu: 8.661 ± 0.924
3.22AlaPhe: 3.22 ± 0.367
7.273AlaGly: 7.273 ± 0.652
1.832AlaHis: 1.832 ± 0.327
5.718AlaIle: 5.718 ± 0.81
6.607AlaLys: 6.607 ± 1.09
8.827AlaLeu: 8.827 ± 0.814
2.831AlaMet: 2.831 ± 0.398
4.108AlaAsn: 4.108 ± 0.551
3.387AlaPro: 3.387 ± 0.477
4.775AlaGln: 4.775 ± 0.597
5.718AlaArg: 5.718 ± 0.662
5.33AlaSer: 5.33 ± 0.638
5.385AlaThr: 5.385 ± 0.701
6.718AlaVal: 6.718 ± 0.719
1.666AlaTrp: 1.666 ± 0.351
2.11AlaTyr: 2.11 ± 0.3
0.0AlaXaa: 0.0 ± 0.0
Cys
0.722CysAla: 0.722 ± 0.22
0.222CysCys: 0.222 ± 0.184
0.722CysAsp: 0.722 ± 0.204
0.444CysGlu: 0.444 ± 0.185
0.278CysPhe: 0.278 ± 0.121
0.944CysGly: 0.944 ± 0.268
0.167CysHis: 0.167 ± 0.099
0.111CysIle: 0.111 ± 0.073
0.111CysLys: 0.111 ± 0.073
0.278CysLeu: 0.278 ± 0.133
0.056CysMet: 0.056 ± 0.055
0.222CysAsn: 0.222 ± 0.098
0.611CysPro: 0.611 ± 0.209
0.222CysGln: 0.222 ± 0.114
0.222CysArg: 0.222 ± 0.101
0.5CysSer: 0.5 ± 0.201
0.444CysThr: 0.444 ± 0.147
0.444CysVal: 0.444 ± 0.168
0.056CysTrp: 0.056 ± 0.057
0.5CysTyr: 0.5 ± 0.146
0.0CysXaa: 0.0 ± 0.0
Asp
6.052AspAla: 6.052 ± 0.546
0.555AspCys: 0.555 ± 0.159
4.997AspAsp: 4.997 ± 0.758
6.607AspGlu: 6.607 ± 1.03
2.054AspPhe: 2.054 ± 0.349
6.662AspGly: 6.662 ± 0.781
0.722AspHis: 0.722 ± 0.227
4.053AspIle: 4.053 ± 0.485
2.72AspLys: 2.72 ± 0.418
6.218AspLeu: 6.218 ± 0.599
1.721AspMet: 1.721 ± 0.345
1.777AspAsn: 1.777 ± 0.263
4.775AspPro: 4.775 ± 0.632
1.832AspGln: 1.832 ± 0.321
3.775AspArg: 3.775 ± 0.46
2.887AspSer: 2.887 ± 0.353
3.72AspThr: 3.72 ± 0.455
3.886AspVal: 3.886 ± 0.515
1.166AspTrp: 1.166 ± 0.226
1.388AspTyr: 1.388 ± 0.345
0.0AspXaa: 0.0 ± 0.0
Glu
7.773GluAla: 7.773 ± 0.728
0.555GluCys: 0.555 ± 0.142
6.496GluAsp: 6.496 ± 0.891
7.439GluGlu: 7.439 ± 1.01
3.775GluPhe: 3.775 ± 0.431
4.775GluGly: 4.775 ± 0.545
2.221GluHis: 2.221 ± 0.357
3.664GluIle: 3.664 ± 0.524
4.053GluLys: 4.053 ± 0.669
5.496GluLeu: 5.496 ± 0.64
2.276GluMet: 2.276 ± 0.333
2.276GluAsn: 2.276 ± 0.363
3.054GluPro: 3.054 ± 0.392
2.887GluGln: 2.887 ± 0.391
5.108GluArg: 5.108 ± 0.704
2.72GluSer: 2.72 ± 0.344
3.886GluThr: 3.886 ± 0.606
4.553GluVal: 4.553 ± 0.658
1.332GluTrp: 1.332 ± 0.302
2.998GluTyr: 2.998 ± 0.402
0.0GluXaa: 0.0 ± 0.0
Phe
2.332PheAla: 2.332 ± 0.357
0.389PheCys: 0.389 ± 0.133
2.276PheAsp: 2.276 ± 0.337
2.665PheGlu: 2.665 ± 0.403
0.888PhePhe: 0.888 ± 0.265
3.109PheGly: 3.109 ± 0.318
0.722PheHis: 0.722 ± 0.207
1.499PheIle: 1.499 ± 0.253
1.666PheLys: 1.666 ± 0.299
2.72PheLeu: 2.72 ± 0.425
0.888PheMet: 0.888 ± 0.204
0.999PheAsn: 0.999 ± 0.208
1.832PhePro: 1.832 ± 0.35
0.944PheGln: 0.944 ± 0.203
2.165PheArg: 2.165 ± 0.276
1.999PheSer: 1.999 ± 0.3
1.721PheThr: 1.721 ± 0.286
1.443PheVal: 1.443 ± 0.287
0.555PheTrp: 0.555 ± 0.194
0.666PheTyr: 0.666 ± 0.189
0.0PheXaa: 0.0 ± 0.0
Gly
6.496GlyAla: 6.496 ± 0.598
0.333GlyCys: 0.333 ± 0.123
4.83GlyAsp: 4.83 ± 0.505
5.496GlyGlu: 5.496 ± 0.517
2.72GlyPhe: 2.72 ± 0.396
5.774GlyGly: 5.774 ± 0.641
1.443GlyHis: 1.443 ± 0.253
4.441GlyIle: 4.441 ± 0.568
4.664GlyLys: 4.664 ± 0.522
5.052GlyLeu: 5.052 ± 0.6
2.221GlyMet: 2.221 ± 0.281
2.665GlyAsn: 2.665 ± 0.433
2.609GlyPro: 2.609 ± 0.387
3.276GlyGln: 3.276 ± 0.332
4.386GlyArg: 4.386 ± 0.534
5.274GlySer: 5.274 ± 0.602
5.552GlyThr: 5.552 ± 0.63
5.996GlyVal: 5.996 ± 0.657
1.888GlyTrp: 1.888 ± 0.299
3.054GlyTyr: 3.054 ± 0.478
0.0GlyXaa: 0.0 ± 0.0
His
1.888HisAla: 1.888 ± 0.261
0.278HisCys: 0.278 ± 0.117
1.11HisAsp: 1.11 ± 0.235
0.833HisGlu: 0.833 ± 0.264
0.722HisPhe: 0.722 ± 0.212
1.555HisGly: 1.555 ± 0.316
0.5HisHis: 0.5 ± 0.151
0.611HisIle: 0.611 ± 0.161
0.833HisLys: 0.833 ± 0.238
1.221HisLeu: 1.221 ± 0.246
0.444HisMet: 0.444 ± 0.146
0.777HisAsn: 0.777 ± 0.212
1.221HisPro: 1.221 ± 0.246
0.444HisGln: 0.444 ± 0.173
1.277HisArg: 1.277 ± 0.255
0.833HisSer: 0.833 ± 0.215
1.555HisThr: 1.555 ± 0.334
1.166HisVal: 1.166 ± 0.279
0.389HisTrp: 0.389 ± 0.156
0.722HisTyr: 0.722 ± 0.222
0.0HisXaa: 0.0 ± 0.0
Ile
5.829IleAla: 5.829 ± 0.632
0.5IleCys: 0.5 ± 0.167
3.831IleAsp: 3.831 ± 0.445
3.831IleGlu: 3.831 ± 0.373
1.221IlePhe: 1.221 ± 0.236
3.942IleGly: 3.942 ± 0.506
0.611IleHis: 0.611 ± 0.218
2.609IleIle: 2.609 ± 0.422
2.443IleLys: 2.443 ± 0.258
3.442IleLeu: 3.442 ± 0.468
0.944IleMet: 0.944 ± 0.259
1.888IleAsn: 1.888 ± 0.327
1.999IlePro: 1.999 ± 0.356
0.944IleGln: 0.944 ± 0.227
3.276IleArg: 3.276 ± 0.58
2.054IleSer: 2.054 ± 0.386
3.72IleThr: 3.72 ± 0.492
3.498IleVal: 3.498 ± 0.458
1.555IleTrp: 1.555 ± 0.43
0.888IleTyr: 0.888 ± 0.249
0.0IleXaa: 0.0 ± 0.0
Lys
6.385LysAla: 6.385 ± 0.726
0.222LysCys: 0.222 ± 0.106
3.22LysAsp: 3.22 ± 0.472
2.998LysGlu: 2.998 ± 0.395
0.999LysPhe: 0.999 ± 0.234
3.498LysGly: 3.498 ± 0.481
0.611LysHis: 0.611 ± 0.152
2.942LysIle: 2.942 ± 0.385
2.998LysLys: 2.998 ± 0.474
4.053LysLeu: 4.053 ± 0.575
1.888LysMet: 1.888 ± 0.323
1.721LysAsn: 1.721 ± 0.294
2.276LysPro: 2.276 ± 0.408
1.943LysGln: 1.943 ± 0.317
2.998LysArg: 2.998 ± 0.437
2.942LysSer: 2.942 ± 0.417
3.553LysThr: 3.553 ± 0.439
3.775LysVal: 3.775 ± 0.385
0.999LysTrp: 0.999 ± 0.207
1.499LysTyr: 1.499 ± 0.292
0.0LysXaa: 0.0 ± 0.0
Leu
7.384LeuAla: 7.384 ± 0.599
0.5LeuCys: 0.5 ± 0.155
4.83LeuAsp: 4.83 ± 0.534
4.941LeuGlu: 4.941 ± 0.447
2.11LeuPhe: 2.11 ± 0.368
5.33LeuGly: 5.33 ± 0.528
1.221LeuHis: 1.221 ± 0.241
3.498LeuIle: 3.498 ± 0.537
4.108LeuLys: 4.108 ± 0.604
5.219LeuLeu: 5.219 ± 0.576
1.943LeuMet: 1.943 ± 0.349
2.72LeuAsn: 2.72 ± 0.379
4.386LeuPro: 4.386 ± 0.579
2.498LeuGln: 2.498 ± 0.392
4.997LeuArg: 4.997 ± 0.474
3.775LeuSer: 3.775 ± 0.493
5.219LeuThr: 5.219 ± 0.576
5.052LeuVal: 5.052 ± 0.549
1.332LeuTrp: 1.332 ± 0.241
1.721LeuTyr: 1.721 ± 0.334
0.0LeuXaa: 0.0 ± 0.0
Met
3.387MetAla: 3.387 ± 0.411
0.056MetCys: 0.056 ± 0.052
1.388MetAsp: 1.388 ± 0.286
1.443MetGlu: 1.443 ± 0.263
0.999MetPhe: 0.999 ± 0.184
1.666MetGly: 1.666 ± 0.312
0.222MetHis: 0.222 ± 0.102
1.499MetIle: 1.499 ± 0.307
0.999MetLys: 0.999 ± 0.232
2.054MetLeu: 2.054 ± 0.334
0.722MetMet: 0.722 ± 0.232
1.277MetAsn: 1.277 ± 0.281
1.721MetPro: 1.721 ± 0.35
0.833MetGln: 0.833 ± 0.229
1.166MetArg: 1.166 ± 0.241
2.11MetSer: 2.11 ± 0.317
1.943MetThr: 1.943 ± 0.324
1.277MetVal: 1.277 ± 0.25
0.5MetTrp: 0.5 ± 0.159
0.333MetTyr: 0.333 ± 0.13
0.0MetXaa: 0.0 ± 0.0
Asn
4.33AsnAla: 4.33 ± 0.717
0.278AsnCys: 0.278 ± 0.104
2.221AsnAsp: 2.221 ± 0.259
2.665AsnGlu: 2.665 ± 0.366
0.833AsnPhe: 0.833 ± 0.239
2.387AsnGly: 2.387 ± 0.429
0.888AsnHis: 0.888 ± 0.227
1.388AsnIle: 1.388 ± 0.321
1.499AsnLys: 1.499 ± 0.285
2.332AsnLeu: 2.332 ± 0.403
0.833AsnMet: 0.833 ± 0.23
1.943AsnAsn: 1.943 ± 0.397
2.11AsnPro: 2.11 ± 0.469
1.388AsnGln: 1.388 ± 0.271
2.498AsnArg: 2.498 ± 0.392
1.499AsnSer: 1.499 ± 0.283
2.72AsnThr: 2.72 ± 0.461
2.165AsnVal: 2.165 ± 0.401
0.611AsnTrp: 0.611 ± 0.194
0.722AsnTyr: 0.722 ± 0.22
0.0AsnXaa: 0.0 ± 0.0
Pro
5.163ProAla: 5.163 ± 0.73
0.056ProCys: 0.056 ± 0.056
3.609ProAsp: 3.609 ± 0.419
5.052ProGlu: 5.052 ± 0.707
1.443ProPhe: 1.443 ± 0.346
3.664ProGly: 3.664 ± 0.518
0.944ProHis: 0.944 ± 0.212
2.332ProIle: 2.332 ± 0.466
3.22ProLys: 3.22 ± 0.466
3.331ProLeu: 3.331 ± 0.351
1.221ProMet: 1.221 ± 0.243
1.332ProAsn: 1.332 ± 0.312
1.666ProPro: 1.666 ± 0.308
1.61ProGln: 1.61 ± 0.271
2.11ProArg: 2.11 ± 0.322
3.054ProSer: 3.054 ± 0.444
3.775ProThr: 3.775 ± 0.561
3.22ProVal: 3.22 ± 0.483
0.833ProTrp: 0.833 ± 0.211
1.721ProTyr: 1.721 ± 0.374
0.0ProXaa: 0.0 ± 0.0
Gln
3.387GlnAla: 3.387 ± 0.4
0.167GlnCys: 0.167 ± 0.087
1.721GlnAsp: 1.721 ± 0.296
2.665GlnGlu: 2.665 ± 0.452
1.277GlnPhe: 1.277 ± 0.262
2.498GlnGly: 2.498 ± 0.306
1.055GlnHis: 1.055 ± 0.237
1.888GlnIle: 1.888 ± 0.371
1.999GlnLys: 1.999 ± 0.261
2.665GlnLeu: 2.665 ± 0.36
1.221GlnMet: 1.221 ± 0.257
1.221GlnAsn: 1.221 ± 0.324
2.054GlnPro: 2.054 ± 0.459
1.166GlnGln: 1.166 ± 0.291
2.665GlnArg: 2.665 ± 0.342
2.054GlnSer: 2.054 ± 0.369
2.332GlnThr: 2.332 ± 0.283
1.832GlnVal: 1.832 ± 0.25
0.777GlnTrp: 0.777 ± 0.221
0.722GlnTyr: 0.722 ± 0.181
0.0GlnXaa: 0.0 ± 0.0
Arg
5.829ArgAla: 5.829 ± 0.59
0.611ArgCys: 0.611 ± 0.226
4.275ArgAsp: 4.275 ± 0.469
3.997ArgGlu: 3.997 ± 0.446
1.999ArgPhe: 1.999 ± 0.314
3.72ArgGly: 3.72 ± 0.425
1.055ArgHis: 1.055 ± 0.253
2.831ArgIle: 2.831 ± 0.374
2.887ArgLys: 2.887 ± 0.501
4.164ArgLeu: 4.164 ± 0.487
1.943ArgMet: 1.943 ± 0.34
1.555ArgAsn: 1.555 ± 0.313
2.942ArgPro: 2.942 ± 0.374
3.054ArgGln: 3.054 ± 0.533
5.441ArgArg: 5.441 ± 0.602
3.109ArgSer: 3.109 ± 0.408
4.441ArgThr: 4.441 ± 0.664
4.275ArgVal: 4.275 ± 0.528
1.443ArgTrp: 1.443 ± 0.337
2.387ArgTyr: 2.387 ± 0.394
0.0ArgXaa: 0.0 ± 0.0
Ser
6.44SerAla: 6.44 ± 0.57
0.5SerCys: 0.5 ± 0.2
2.942SerAsp: 2.942 ± 0.518
4.275SerGlu: 4.275 ± 0.528
1.943SerPhe: 1.943 ± 0.354
6.218SerGly: 6.218 ± 0.49
0.999SerHis: 0.999 ± 0.245
1.999SerIle: 1.999 ± 0.27
2.72SerLys: 2.72 ± 0.415
3.553SerLeu: 3.553 ± 0.363
1.277SerMet: 1.277 ± 0.324
1.61SerAsn: 1.61 ± 0.333
2.776SerPro: 2.776 ± 0.42
1.777SerGln: 1.777 ± 0.312
2.72SerArg: 2.72 ± 0.398
2.831SerSer: 2.831 ± 0.373
2.554SerThr: 2.554 ± 0.415
3.387SerVal: 3.387 ± 0.396
1.166SerTrp: 1.166 ± 0.227
1.61SerTyr: 1.61 ± 0.318
0.0SerXaa: 0.0 ± 0.0
Thr
5.829ThrAla: 5.829 ± 0.769
0.5ThrCys: 0.5 ± 0.184
4.553ThrAsp: 4.553 ± 0.468
4.608ThrGlu: 4.608 ± 0.42
1.721ThrPhe: 1.721 ± 0.297
5.496ThrGly: 5.496 ± 0.686
1.277ThrHis: 1.277 ± 0.347
3.054ThrIle: 3.054 ± 0.463
3.054ThrLys: 3.054 ± 0.479
3.72ThrLeu: 3.72 ± 0.368
1.055ThrMet: 1.055 ± 0.22
2.332ThrAsn: 2.332 ± 0.355
4.219ThrPro: 4.219 ± 0.841
1.999ThrGln: 1.999 ± 0.281
4.441ThrArg: 4.441 ± 0.508
3.054ThrSer: 3.054 ± 0.392
4.219ThrThr: 4.219 ± 0.668
5.108ThrVal: 5.108 ± 0.399
1.388ThrTrp: 1.388 ± 0.233
2.276ThrTyr: 2.276 ± 0.361
0.0ThrXaa: 0.0 ± 0.0
Val
7.217ValAla: 7.217 ± 0.571
0.555ValCys: 0.555 ± 0.202
4.053ValAsp: 4.053 ± 0.384
5.052ValGlu: 5.052 ± 0.483
1.332ValPhe: 1.332 ± 0.296
5.829ValGly: 5.829 ± 0.76
0.611ValHis: 0.611 ± 0.168
3.054ValIle: 3.054 ± 0.363
2.998ValLys: 2.998 ± 0.303
5.219ValLeu: 5.219 ± 0.641
0.999ValMet: 0.999 ± 0.23
2.665ValAsn: 2.665 ± 0.419
3.553ValPro: 3.553 ± 0.79
2.221ValGln: 2.221 ± 0.396
3.831ValArg: 3.831 ± 0.558
4.608ValSer: 4.608 ± 0.577
4.553ValThr: 4.553 ± 0.587
4.886ValVal: 4.886 ± 0.786
1.555ValTrp: 1.555 ± 0.351
2.276ValTyr: 2.276 ± 0.308
0.0ValXaa: 0.0 ± 0.0
Trp
1.721TrpAla: 1.721 ± 0.349
0.278TrpCys: 0.278 ± 0.114
1.388TrpAsp: 1.388 ± 0.274
2.165TrpGlu: 2.165 ± 0.365
1.11TrpPhe: 1.11 ± 0.269
1.277TrpGly: 1.277 ± 0.266
0.666TrpHis: 0.666 ± 0.203
0.833TrpIle: 0.833 ± 0.196
0.666TrpLys: 0.666 ± 0.198
1.721TrpLeu: 1.721 ± 0.305
0.278TrpMet: 0.278 ± 0.114
0.833TrpAsn: 0.833 ± 0.359
0.999TrpPro: 0.999 ± 0.276
0.555TrpGln: 0.555 ± 0.175
1.166TrpArg: 1.166 ± 0.326
0.999TrpSer: 0.999 ± 0.207
0.999TrpThr: 0.999 ± 0.311
1.388TrpVal: 1.388 ± 0.245
0.333TrpTrp: 0.333 ± 0.123
0.666TrpTyr: 0.666 ± 0.196
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.554TyrAla: 2.554 ± 0.425
0.333TyrCys: 0.333 ± 0.169
2.942TyrAsp: 2.942 ± 0.468
1.499TyrGlu: 1.499 ± 0.297
0.833TyrPhe: 0.833 ± 0.168
2.554TyrGly: 2.554 ± 0.338
0.611TyrHis: 0.611 ± 0.178
0.999TyrIle: 0.999 ± 0.194
1.277TyrLys: 1.277 ± 0.233
1.499TyrLeu: 1.499 ± 0.268
0.777TyrMet: 0.777 ± 0.206
1.388TyrAsn: 1.388 ± 0.346
1.166TyrPro: 1.166 ± 0.279
0.999TyrGln: 0.999 ± 0.265
2.054TyrArg: 2.054 ± 0.362
1.666TyrSer: 1.666 ± 0.283
1.61TyrThr: 1.61 ± 0.274
2.998TyrVal: 2.998 ± 0.473
0.5TyrTrp: 0.5 ± 0.163
0.666TyrTyr: 0.666 ± 0.199
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 83 proteins (18013 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski