Common bean-associated gemycircularvirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; unclassified Gemycircularvirus

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C8YZ07|A0A1C8YZ07_9VIRU Capsid protein OS=Common bean-associated gemycircularvirus OX=1897538 PE=4 SV=1
MM1 pKa = 6.83PAKK4 pKa = 10.54YY5 pKa = 9.99KK6 pKa = 11.17LNDD9 pKa = 3.34EE10 pKa = 4.28QFFMLTYY17 pKa = 8.23PTIPDD22 pKa = 3.68GFDD25 pKa = 3.07TDD27 pKa = 3.98GLINVLEE34 pKa = 4.13RR35 pKa = 11.84LGCNYY40 pKa = 10.45RR41 pKa = 11.84IGRR44 pKa = 11.84EE45 pKa = 3.79LHH47 pKa = 6.16QDD49 pKa = 3.95GKK51 pKa = 9.65PHH53 pKa = 6.18LHH55 pKa = 7.03AMLCFDD61 pKa = 4.92EE62 pKa = 5.88PYY64 pKa = 10.44TDD66 pKa = 3.55GDD68 pKa = 3.29ARR70 pKa = 11.84ATFKK74 pKa = 10.69IGTRR78 pKa = 11.84VPNIRR83 pKa = 11.84VRR85 pKa = 11.84RR86 pKa = 11.84VKK88 pKa = 10.46PEE90 pKa = 3.96RR91 pKa = 11.84GWDD94 pKa = 3.59YY95 pKa = 11.17VGKK98 pKa = 9.63HH99 pKa = 5.89AGTKK103 pKa = 8.06EE104 pKa = 3.4GHH106 pKa = 6.39YY107 pKa = 10.45IVGEE111 pKa = 3.92KK112 pKa = 9.01GTRR115 pKa = 11.84PGGDD119 pKa = 3.14EE120 pKa = 4.65DD121 pKa = 3.72STEE124 pKa = 4.08RR125 pKa = 11.84SSNDD129 pKa = 2.27AWFEE133 pKa = 4.08IILARR138 pKa = 11.84TRR140 pKa = 11.84EE141 pKa = 4.02EE142 pKa = 4.24FFDD145 pKa = 3.61SAARR149 pKa = 11.84LAPRR153 pKa = 11.84QLACNFSSLTAYY165 pKa = 10.43ADD167 pKa = 3.1WKK169 pKa = 9.37YY170 pKa = 11.01RR171 pKa = 11.84PVAAAYY177 pKa = 9.4SNPDD181 pKa = 3.11GEE183 pKa = 4.29FSVPFEE189 pKa = 4.33LKK191 pKa = 10.48DD192 pKa = 3.42WVDD195 pKa = 3.86DD196 pKa = 4.02NVRR199 pKa = 11.84GCSGRR204 pKa = 11.84PKK206 pKa = 10.77GLVLFGATRR215 pKa = 11.84LGKK218 pKa = 7.65TVWARR223 pKa = 11.84SLGNHH228 pKa = 6.22YY229 pKa = 10.94YY230 pKa = 10.72CGGLWNLADD239 pKa = 3.85FDD241 pKa = 4.31EE242 pKa = 4.69TVEE245 pKa = 3.99YY246 pKa = 10.68AIFDD250 pKa = 3.91DD251 pKa = 4.15MVNGLRR257 pKa = 11.84AGYY260 pKa = 8.54FQYY263 pKa = 11.18KK264 pKa = 9.87DD265 pKa = 3.33WLGGQYY271 pKa = 10.9EE272 pKa = 4.5FMVQDD277 pKa = 3.39KK278 pKa = 10.52YY279 pKa = 10.9KK280 pKa = 10.72GKK282 pKa = 10.48RR283 pKa = 11.84RR284 pKa = 11.84VKK286 pKa = 8.91WGKK289 pKa = 9.53PSIFICNRR297 pKa = 11.84DD298 pKa = 3.38PRR300 pKa = 11.84DD301 pKa = 3.66EE302 pKa = 5.03IGLDD306 pKa = 3.44DD307 pKa = 4.42HH308 pKa = 6.89NKK310 pKa = 10.55SKK312 pKa = 10.91IEE314 pKa = 3.86WDD316 pKa = 3.12WMEE319 pKa = 4.28EE320 pKa = 3.59NCLFYY325 pKa = 10.65EE326 pKa = 3.95LRR328 pKa = 11.84EE329 pKa = 4.24PIFRR333 pKa = 11.84ASTEE337 pKa = 3.81

Molecular weight: 38.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C8YZ07|A0A1C8YZ07_9VIRU Capsid protein OS=Common bean-associated gemycircularvirus OX=1897538 PE=4 SV=1
MM1 pKa = 7.75PVRR4 pKa = 11.84NRR6 pKa = 11.84NFPRR10 pKa = 11.84GWRR13 pKa = 11.84SRR15 pKa = 11.84YY16 pKa = 8.66RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 10.21NVLNITSRR27 pKa = 11.84KK28 pKa = 9.15KK29 pKa = 10.27QDD31 pKa = 3.19TMLPWTNATASSVSGGSTFTNQAAVFVGGNTYY63 pKa = 10.4ILPWCCTARR72 pKa = 11.84DD73 pKa = 3.85NILANGSPATLFSQSSRR90 pKa = 11.84TATQCYY96 pKa = 7.86MRR98 pKa = 11.84GLKK101 pKa = 9.88EE102 pKa = 4.22RR103 pKa = 11.84IQVQTSTGMPWQWRR117 pKa = 11.84RR118 pKa = 11.84ICFTLKK124 pKa = 10.67GDD126 pKa = 3.74TLTQYY131 pKa = 11.42NEE133 pKa = 3.63TAYY136 pKa = 9.89KK137 pKa = 10.12IIAEE141 pKa = 4.33NSSGWARR148 pKa = 11.84VVSNVGDD155 pKa = 3.69KK156 pKa = 11.45SNLAVAINNLVFRR169 pKa = 11.84GQVEE173 pKa = 4.5SDD175 pKa = 2.52WKK177 pKa = 11.13AFFNAKK183 pKa = 9.79VDD185 pKa = 3.87DD186 pKa = 4.09SRR188 pKa = 11.84VSLKK192 pKa = 10.16YY193 pKa = 10.65DD194 pKa = 2.81KK195 pKa = 10.44TIYY198 pKa = 10.49VRR200 pKa = 11.84TGNNFGSMRR209 pKa = 11.84DD210 pKa = 3.27YY211 pKa = 11.34HH212 pKa = 6.81FWHH215 pKa = 6.81KK216 pKa = 10.46MNSTLIYY223 pKa = 10.44DD224 pKa = 4.02DD225 pKa = 5.62DD226 pKa = 4.13EE227 pKa = 7.16AGVSMVPSYY236 pKa = 11.53YY237 pKa = 9.63STEE240 pKa = 3.96SRR242 pKa = 11.84QGMGDD247 pKa = 3.72YY248 pKa = 10.71YY249 pKa = 11.39VIDD252 pKa = 4.91IISAGTGATSSDD264 pKa = 2.86QMTFNPQATLYY275 pKa = 8.14WHH277 pKa = 6.53EE278 pKa = 4.17RR279 pKa = 3.47

Molecular weight: 31.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

616

279

337

308.0

35.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.656 ± 0.095

1.786 ± 0.217

7.143 ± 1.09

5.032 ± 1.558

4.87 ± 0.572

8.117 ± 0.807

1.623 ± 0.338

4.383 ± 0.171

5.195 ± 0.552

6.006 ± 0.831

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.435 ± 0.488

5.519 ± 1.018

3.896 ± 0.635

2.922 ± 0.851

7.792 ± 0.164

6.494 ± 1.965

6.494 ± 1.523

5.519 ± 0.575

3.084 ± 0.087

5.032 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski