Bacillus salacetis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4589 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A1RD64|A0A3A1RD64_9BACI DUF2553 family protein OS=Bacillus salacetis OX=2315464 GN=D3H55_00630 PE=4 SV=1
MM1 pKa = 7.01NLEE4 pKa = 3.72WFDD7 pKa = 3.8RR8 pKa = 11.84VSGEE12 pKa = 4.11LQDD15 pKa = 4.0HH16 pKa = 6.73LEE18 pKa = 4.59SICEE22 pKa = 4.09KK23 pKa = 10.33YY24 pKa = 10.79DD25 pKa = 3.92QIGQMNIDD33 pKa = 4.24RR34 pKa = 11.84GSKK37 pKa = 9.0HH38 pKa = 5.97PRR40 pKa = 11.84IEE42 pKa = 4.63FYY44 pKa = 11.28VEE46 pKa = 3.9TEE48 pKa = 3.75DD49 pKa = 4.03QEE51 pKa = 5.45RR52 pKa = 11.84DD53 pKa = 3.9FFCTLLFDD61 pKa = 5.09PYY63 pKa = 11.21NEE65 pKa = 3.93EE66 pKa = 5.09FYY68 pKa = 10.67MEE70 pKa = 5.19SYY72 pKa = 10.78DD73 pKa = 3.58IDD75 pKa = 3.68YY76 pKa = 10.37GQSLKK81 pKa = 10.59IILPDD86 pKa = 3.32IEE88 pKa = 5.78DD89 pKa = 3.37IVDD92 pKa = 3.65AVHH95 pKa = 6.95EE96 pKa = 4.38SLHH99 pKa = 7.2DD100 pKa = 3.81YY101 pKa = 11.61LNMEE105 pKa = 4.4EE106 pKa = 4.15VDD108 pKa = 5.46FYY110 pKa = 11.65DD111 pKa = 5.43DD112 pKa = 3.15TDD114 pKa = 3.97EE115 pKa = 4.92YY116 pKa = 11.18DD117 pKa = 3.34DD118 pKa = 5.44HH119 pKa = 8.83IDD121 pKa = 4.31YY122 pKa = 10.82IVDD125 pKa = 3.58DD126 pKa = 5.13EE127 pKa = 5.35GPVHH131 pKa = 6.23YY132 pKa = 10.92VVDD135 pKa = 4.6DD136 pKa = 3.77YY137 pKa = 11.81DD138 pKa = 4.11YY139 pKa = 11.78EE140 pKa = 4.36EE141 pKa = 5.38YY142 pKa = 11.06GDD144 pKa = 3.81EE145 pKa = 4.04DD146 pKa = 4.87HH147 pKa = 7.28ILEE150 pKa = 5.02EE151 pKa = 5.27IDD153 pKa = 4.09VAWEE157 pKa = 4.27TPEE160 pKa = 3.83VTAFLHH166 pKa = 5.88GDD168 pKa = 3.69EE169 pKa = 5.35VEE171 pKa = 4.08VTYY174 pKa = 10.81QFGIITEE181 pKa = 4.32TGDD184 pKa = 3.33GVLRR188 pKa = 11.84RR189 pKa = 11.84VNRR192 pKa = 11.84LRR194 pKa = 11.84AEE196 pKa = 4.33DD197 pKa = 4.04GEE199 pKa = 4.58LIKK202 pKa = 11.12DD203 pKa = 3.4EE204 pKa = 4.41SHH206 pKa = 7.38FIFSKK211 pKa = 10.96EE212 pKa = 3.74EE213 pKa = 3.86ANTIIAMIASHH224 pKa = 6.34SDD226 pKa = 2.99KK227 pKa = 10.94LSEE230 pKa = 4.4FSFDD234 pKa = 3.23

Molecular weight: 27.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A1QYA9|A0A3A1QYA9_9BACI ABC transporter ATP-binding protein OS=Bacillus salacetis OX=2315464 GN=D3H55_13470 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.55RR3 pKa = 11.84TFQPNSRR10 pKa = 11.84KK11 pKa = 9.5RR12 pKa = 11.84SKK14 pKa = 10.67NHH16 pKa = 4.97GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 10.48NGRR28 pKa = 11.84KK29 pKa = 8.81VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4589

0

4589

1258239

26

2662

274.2

30.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.923 ± 0.041

0.673 ± 0.011

5.286 ± 0.032

8.033 ± 0.048

4.687 ± 0.032

7.101 ± 0.037

2.003 ± 0.019

7.493 ± 0.035

6.878 ± 0.035

9.767 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.827 ± 0.02

4.269 ± 0.028

3.599 ± 0.02

3.53 ± 0.025

4.197 ± 0.029

6.342 ± 0.03

5.109 ± 0.024

6.704 ± 0.031

1.067 ± 0.014

3.512 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski