Amino acid dipepetide frequency for Shigella phage 75/02 Stx

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.957AlaAla: 7.957 ± 0.747
1.447AlaCys: 1.447 ± 0.39
5.509AlaAsp: 5.509 ± 0.496
8.513AlaGlu: 8.513 ± 0.787
3.561AlaPhe: 3.561 ± 0.609
7.178AlaGly: 7.178 ± 0.776
1.335AlaHis: 1.335 ± 0.249
3.839AlaIle: 3.839 ± 0.435
4.841AlaLys: 4.841 ± 0.499
6.176AlaLeu: 6.176 ± 0.546
3.45AlaMet: 3.45 ± 0.446
2.615AlaAsn: 2.615 ± 0.44
2.838AlaPro: 2.838 ± 0.317
5.119AlaGln: 5.119 ± 0.756
5.898AlaArg: 5.898 ± 0.708
5.954AlaSer: 5.954 ± 0.541
5.731AlaThr: 5.731 ± 0.547
6.232AlaVal: 6.232 ± 0.658
1.558AlaTrp: 1.558 ± 0.34
1.725AlaTyr: 1.725 ± 0.333
0.0AlaXaa: 0.0 ± 0.0
Cys
1.113CysAla: 1.113 ± 0.295
0.334CysCys: 0.334 ± 0.145
0.389CysAsp: 0.389 ± 0.163
0.723CysGlu: 0.723 ± 0.228
0.445CysPhe: 0.445 ± 0.152
1.113CysGly: 1.113 ± 0.338
0.389CysHis: 0.389 ± 0.144
0.389CysIle: 0.389 ± 0.133
0.668CysLys: 0.668 ± 0.221
0.556CysLeu: 0.556 ± 0.184
0.278CysMet: 0.278 ± 0.139
0.445CysAsn: 0.445 ± 0.17
0.556CysPro: 0.556 ± 0.177
0.334CysGln: 0.334 ± 0.159
1.391CysArg: 1.391 ± 0.3
1.002CysSer: 1.002 ± 0.285
0.668CysThr: 0.668 ± 0.218
1.002CysVal: 1.002 ± 0.293
0.111CysTrp: 0.111 ± 0.076
0.389CysTyr: 0.389 ± 0.155
0.0CysXaa: 0.0 ± 0.0
Asp
5.675AspAla: 5.675 ± 0.653
0.556AspCys: 0.556 ± 0.169
4.451AspAsp: 4.451 ± 0.61
4.563AspGlu: 4.563 ± 0.579
1.836AspPhe: 1.836 ± 0.324
4.563AspGly: 4.563 ± 0.431
0.946AspHis: 0.946 ± 0.237
3.728AspIle: 3.728 ± 0.453
4.006AspLys: 4.006 ± 0.412
3.895AspLeu: 3.895 ± 0.461
1.391AspMet: 1.391 ± 0.245
2.949AspAsn: 2.949 ± 0.361
2.782AspPro: 2.782 ± 0.313
1.502AspGln: 1.502 ± 0.382
3.339AspArg: 3.339 ± 0.375
3.45AspSer: 3.45 ± 0.445
3.005AspThr: 3.005 ± 0.419
4.062AspVal: 4.062 ± 0.481
1.28AspTrp: 1.28 ± 0.341
1.947AspTyr: 1.947 ± 0.28
0.0AspXaa: 0.0 ± 0.0
Glu
6.51GluAla: 6.51 ± 0.5
1.057GluCys: 1.057 ± 0.296
2.782GluAsp: 2.782 ± 0.377
4.229GluGlu: 4.229 ± 0.405
2.337GluPhe: 2.337 ± 0.421
4.284GluGly: 4.284 ± 0.457
0.835GluHis: 0.835 ± 0.165
4.284GluIle: 4.284 ± 0.652
5.063GluLys: 5.063 ± 0.636
5.787GluLeu: 5.787 ± 0.599
2.393GluMet: 2.393 ± 0.347
3.116GluAsn: 3.116 ± 0.467
1.836GluPro: 1.836 ± 0.401
4.451GluGln: 4.451 ± 0.496
4.952GluArg: 4.952 ± 0.582
3.672GluSer: 3.672 ± 0.46
3.951GluThr: 3.951 ± 0.554
4.563GluVal: 4.563 ± 0.624
1.168GluTrp: 1.168 ± 0.216
2.504GluTyr: 2.504 ± 0.363
0.0GluXaa: 0.0 ± 0.0
Phe
2.726PheAla: 2.726 ± 0.406
0.501PheCys: 0.501 ± 0.176
1.725PheAsp: 1.725 ± 0.318
1.502PheGlu: 1.502 ± 0.4
1.168PhePhe: 1.168 ± 0.283
2.114PheGly: 2.114 ± 0.264
0.723PheHis: 0.723 ± 0.184
2.059PheIle: 2.059 ± 0.322
1.391PheLys: 1.391 ± 0.218
2.059PheLeu: 2.059 ± 0.325
1.002PheMet: 1.002 ± 0.228
1.614PheAsn: 1.614 ± 0.294
1.224PhePro: 1.224 ± 0.269
0.779PheGln: 0.779 ± 0.213
2.56PheArg: 2.56 ± 0.308
3.116PheSer: 3.116 ± 0.436
2.281PheThr: 2.281 ± 0.368
2.782PheVal: 2.782 ± 0.444
0.779PheTrp: 0.779 ± 0.199
0.946PheTyr: 0.946 ± 0.219
0.0PheXaa: 0.0 ± 0.0
Gly
5.62GlyAla: 5.62 ± 0.948
0.501GlyCys: 0.501 ± 0.187
4.507GlyAsp: 4.507 ± 0.445
5.175GlyGlu: 5.175 ± 0.801
2.726GlyPhe: 2.726 ± 0.426
5.286GlyGly: 5.286 ± 0.746
0.779GlyHis: 0.779 ± 0.217
4.173GlyIle: 4.173 ± 0.647
5.397GlyLys: 5.397 ± 0.811
4.897GlyLeu: 4.897 ± 0.486
2.226GlyMet: 2.226 ± 0.296
3.227GlyAsn: 3.227 ± 0.375
2.838GlyPro: 2.838 ± 0.946
2.838GlyGln: 2.838 ± 0.412
4.507GlyArg: 4.507 ± 0.493
3.672GlySer: 3.672 ± 0.547
4.006GlyThr: 4.006 ± 0.501
5.453GlyVal: 5.453 ± 0.495
0.946GlyTrp: 0.946 ± 0.216
2.114GlyTyr: 2.114 ± 0.352
0.0GlyXaa: 0.0 ± 0.0
His
2.17HisAla: 2.17 ± 0.3
0.334HisCys: 0.334 ± 0.168
1.002HisAsp: 1.002 ± 0.203
0.946HisGlu: 0.946 ± 0.236
0.779HisPhe: 0.779 ± 0.251
1.28HisGly: 1.28 ± 0.298
0.389HisHis: 0.389 ± 0.134
0.89HisIle: 0.89 ± 0.222
0.668HisLys: 0.668 ± 0.208
1.502HisLeu: 1.502 ± 0.354
0.278HisMet: 0.278 ± 0.111
1.224HisAsn: 1.224 ± 0.209
0.723HisPro: 0.723 ± 0.249
0.556HisGln: 0.556 ± 0.208
0.89HisArg: 0.89 ± 0.193
1.113HisSer: 1.113 ± 0.216
1.002HisThr: 1.002 ± 0.206
0.779HisVal: 0.779 ± 0.205
0.111HisTrp: 0.111 ± 0.092
0.723HisTyr: 0.723 ± 0.221
0.0HisXaa: 0.0 ± 0.0
Ile
5.23IleAla: 5.23 ± 0.518
0.835IleCys: 0.835 ± 0.234
3.784IleAsp: 3.784 ± 0.483
2.838IleGlu: 2.838 ± 0.473
1.224IlePhe: 1.224 ± 0.256
2.671IleGly: 2.671 ± 0.508
0.723IleHis: 0.723 ± 0.172
2.56IleIle: 2.56 ± 0.447
3.116IleLys: 3.116 ± 0.404
3.561IleLeu: 3.561 ± 0.522
1.224IleMet: 1.224 ± 0.222
2.56IleAsn: 2.56 ± 0.432
2.893IlePro: 2.893 ± 0.424
2.17IleGln: 2.17 ± 0.25
4.507IleArg: 4.507 ± 0.484
4.173IleSer: 4.173 ± 0.482
4.062IleThr: 4.062 ± 0.684
2.059IleVal: 2.059 ± 0.354
0.278IleTrp: 0.278 ± 0.111
1.947IleTyr: 1.947 ± 0.345
0.0IleXaa: 0.0 ± 0.0
Lys
5.453LysAla: 5.453 ± 0.579
0.501LysCys: 0.501 ± 0.172
3.116LysAsp: 3.116 ± 0.395
3.784LysGlu: 3.784 ± 0.427
1.447LysPhe: 1.447 ± 0.271
4.173LysGly: 4.173 ± 0.812
1.224LysHis: 1.224 ± 0.247
3.45LysIle: 3.45 ± 0.466
4.451LysLys: 4.451 ± 0.519
5.063LysLeu: 5.063 ± 0.598
1.502LysMet: 1.502 ± 0.263
3.172LysAsn: 3.172 ± 0.38
2.393LysPro: 2.393 ± 0.298
2.893LysGln: 2.893 ± 0.4
3.227LysArg: 3.227 ± 0.592
3.283LysSer: 3.283 ± 0.429
4.062LysThr: 4.062 ± 0.408
3.505LysVal: 3.505 ± 0.342
0.779LysTrp: 0.779 ± 0.214
1.502LysTyr: 1.502 ± 0.27
0.0LysXaa: 0.0 ± 0.0
Leu
8.179LeuAla: 8.179 ± 0.702
1.28LeuCys: 1.28 ± 0.298
4.006LeuAsp: 4.006 ± 0.449
4.34LeuGlu: 4.34 ± 0.486
2.226LeuPhe: 2.226 ± 0.321
4.173LeuGly: 4.173 ± 0.486
1.725LeuHis: 1.725 ± 0.334
3.561LeuIle: 3.561 ± 0.518
4.34LeuLys: 4.34 ± 0.501
7.233LeuLeu: 7.233 ± 0.598
2.003LeuMet: 2.003 ± 0.395
4.173LeuAsn: 4.173 ± 0.514
3.561LeuPro: 3.561 ± 0.467
3.005LeuGln: 3.005 ± 0.586
5.509LeuArg: 5.509 ± 0.655
5.564LeuSer: 5.564 ± 0.52
4.674LeuThr: 4.674 ± 0.486
3.951LeuVal: 3.951 ± 0.491
0.668LeuTrp: 0.668 ± 0.211
2.281LeuTyr: 2.281 ± 0.326
0.0LeuXaa: 0.0 ± 0.0
Met
3.172MetAla: 3.172 ± 0.368
0.111MetCys: 0.111 ± 0.08
1.558MetAsp: 1.558 ± 0.265
1.558MetGlu: 1.558 ± 0.306
0.723MetPhe: 0.723 ± 0.161
1.558MetGly: 1.558 ± 0.331
0.334MetHis: 0.334 ± 0.109
1.224MetIle: 1.224 ± 0.252
2.003MetLys: 2.003 ± 0.295
1.725MetLeu: 1.725 ± 0.281
0.946MetMet: 0.946 ± 0.224
1.725MetAsn: 1.725 ± 0.281
1.447MetPro: 1.447 ± 0.296
1.447MetGln: 1.447 ± 0.209
1.447MetArg: 1.447 ± 0.263
2.504MetSer: 2.504 ± 0.337
2.114MetThr: 2.114 ± 0.28
1.669MetVal: 1.669 ± 0.305
0.167MetTrp: 0.167 ± 0.099
0.612MetTyr: 0.612 ± 0.187
0.0MetXaa: 0.0 ± 0.0
Asn
4.73AsnAla: 4.73 ± 0.573
0.668AsnCys: 0.668 ± 0.207
2.226AsnAsp: 2.226 ± 0.467
3.617AsnGlu: 3.617 ± 0.546
1.224AsnPhe: 1.224 ± 0.234
3.617AsnGly: 3.617 ± 0.479
1.113AsnHis: 1.113 ± 0.267
2.726AsnIle: 2.726 ± 0.393
2.393AsnLys: 2.393 ± 0.359
3.394AsnLeu: 3.394 ± 0.34
0.946AsnMet: 0.946 ± 0.215
1.947AsnAsn: 1.947 ± 0.415
2.281AsnPro: 2.281 ± 0.366
1.781AsnGln: 1.781 ± 0.356
2.671AsnArg: 2.671 ± 0.534
2.615AsnSer: 2.615 ± 0.422
1.836AsnThr: 1.836 ± 0.303
2.281AsnVal: 2.281 ± 0.359
0.612AsnTrp: 0.612 ± 0.17
1.447AsnTyr: 1.447 ± 0.296
0.0AsnXaa: 0.0 ± 0.0
Pro
2.949ProAla: 2.949 ± 0.484
0.334ProCys: 0.334 ± 0.118
4.897ProAsp: 4.897 ± 0.593
5.62ProGlu: 5.62 ± 0.729
1.28ProPhe: 1.28 ± 0.206
3.339ProGly: 3.339 ± 0.432
0.556ProHis: 0.556 ± 0.187
0.89ProIle: 0.89 ± 0.231
2.114ProLys: 2.114 ± 0.364
2.671ProLeu: 2.671 ± 0.389
0.668ProMet: 0.668 ± 0.162
0.835ProAsn: 0.835 ± 0.185
1.614ProPro: 1.614 ± 0.293
2.059ProGln: 2.059 ± 0.324
1.447ProArg: 1.447 ± 0.325
2.671ProSer: 2.671 ± 0.379
1.614ProThr: 1.614 ± 0.303
4.785ProVal: 4.785 ± 0.468
0.556ProTrp: 0.556 ± 0.172
1.224ProTyr: 1.224 ± 0.245
0.0ProXaa: 0.0 ± 0.0
Gln
4.396GlnAla: 4.396 ± 0.599
0.501GlnCys: 0.501 ± 0.167
2.337GlnAsp: 2.337 ± 0.372
2.893GlnGlu: 2.893 ± 0.438
1.502GlnPhe: 1.502 ± 0.288
2.838GlnGly: 2.838 ± 0.486
0.668GlnHis: 0.668 ± 0.139
2.226GlnIle: 2.226 ± 0.391
3.45GlnLys: 3.45 ± 0.575
3.005GlnLeu: 3.005 ± 0.479
1.447GlnMet: 1.447 ± 0.312
1.892GlnAsn: 1.892 ± 0.368
2.003GlnPro: 2.003 ± 0.427
4.173GlnGln: 4.173 ± 0.842
3.505GlnArg: 3.505 ± 0.591
2.393GlnSer: 2.393 ± 0.275
2.337GlnThr: 2.337 ± 0.348
2.671GlnVal: 2.671 ± 0.504
0.612GlnTrp: 0.612 ± 0.163
1.28GlnTyr: 1.28 ± 0.243
0.0GlnXaa: 0.0 ± 0.0
Arg
4.451ArgAla: 4.451 ± 0.465
0.556ArgCys: 0.556 ± 0.266
3.561ArgAsp: 3.561 ± 0.55
5.063ArgGlu: 5.063 ± 0.75
2.393ArgPhe: 2.393 ± 0.375
4.396ArgGly: 4.396 ± 0.491
1.725ArgHis: 1.725 ± 0.258
3.895ArgIle: 3.895 ± 0.65
4.118ArgLys: 4.118 ± 0.493
5.175ArgLeu: 5.175 ± 0.446
2.226ArgMet: 2.226 ± 0.334
3.339ArgAsn: 3.339 ± 0.431
1.892ArgPro: 1.892 ± 0.336
3.06ArgGln: 3.06 ± 0.351
5.731ArgArg: 5.731 ± 0.585
3.505ArgSer: 3.505 ± 0.43
3.505ArgThr: 3.505 ± 0.526
4.451ArgVal: 4.451 ± 0.56
1.224ArgTrp: 1.224 ± 0.266
2.671ArgTyr: 2.671 ± 0.433
0.0ArgXaa: 0.0 ± 0.0
Ser
5.842SerAla: 5.842 ± 0.549
0.723SerCys: 0.723 ± 0.221
4.229SerAsp: 4.229 ± 0.393
4.451SerGlu: 4.451 ± 0.383
1.947SerPhe: 1.947 ± 0.239
6.121SerGly: 6.121 ± 0.628
1.057SerHis: 1.057 ± 0.285
2.337SerIle: 2.337 ± 0.318
2.671SerLys: 2.671 ± 0.397
6.343SerLeu: 6.343 ± 0.643
1.558SerMet: 1.558 ± 0.273
2.504SerAsn: 2.504 ± 0.351
3.394SerPro: 3.394 ± 0.481
2.838SerGln: 2.838 ± 0.482
3.784SerArg: 3.784 ± 0.501
3.06SerSer: 3.06 ± 0.607
3.005SerThr: 3.005 ± 0.406
4.284SerVal: 4.284 ± 0.599
0.89SerTrp: 0.89 ± 0.217
1.502SerTyr: 1.502 ± 0.273
0.0SerXaa: 0.0 ± 0.0
Thr
5.787ThrAla: 5.787 ± 0.504
0.445ThrCys: 0.445 ± 0.158
3.172ThrAsp: 3.172 ± 0.467
4.396ThrGlu: 4.396 ± 0.422
2.059ThrPhe: 2.059 ± 0.453
5.509ThrGly: 5.509 ± 0.514
1.113ThrHis: 1.113 ± 0.237
3.505ThrIle: 3.505 ± 0.477
2.56ThrLys: 2.56 ± 0.366
4.841ThrLeu: 4.841 ± 0.5
0.89ThrMet: 0.89 ± 0.199
1.725ThrAsn: 1.725 ± 0.274
3.45ThrPro: 3.45 ± 0.396
2.337ThrGln: 2.337 ± 0.382
2.949ThrArg: 2.949 ± 0.441
3.784ThrSer: 3.784 ± 0.557
3.617ThrThr: 3.617 ± 0.466
3.895ThrVal: 3.895 ± 0.6
0.946ThrTrp: 0.946 ± 0.189
1.224ThrTyr: 1.224 ± 0.302
0.0ThrXaa: 0.0 ± 0.0
Val
6.51ValAla: 6.51 ± 0.611
1.057ValCys: 1.057 ± 0.256
4.284ValAsp: 4.284 ± 0.464
3.895ValGlu: 3.895 ± 0.457
2.504ValPhe: 2.504 ± 0.372
3.45ValGly: 3.45 ± 0.519
1.002ValHis: 1.002 ± 0.201
4.062ValIle: 4.062 ± 0.499
3.505ValLys: 3.505 ± 0.379
5.397ValLeu: 5.397 ± 0.647
1.836ValMet: 1.836 ± 0.302
3.116ValAsn: 3.116 ± 0.317
2.504ValPro: 2.504 ± 0.436
2.337ValGln: 2.337 ± 0.353
4.897ValArg: 4.897 ± 0.933
4.618ValSer: 4.618 ± 0.556
4.34ValThr: 4.34 ± 0.564
4.451ValVal: 4.451 ± 0.374
0.556ValTrp: 0.556 ± 0.171
1.558ValTyr: 1.558 ± 0.256
0.0ValXaa: 0.0 ± 0.0
Trp
0.723TrpAla: 0.723 ± 0.215
0.167TrpCys: 0.167 ± 0.107
0.501TrpAsp: 0.501 ± 0.172
0.556TrpGlu: 0.556 ± 0.149
0.556TrpPhe: 0.556 ± 0.169
0.779TrpGly: 0.779 ± 0.213
0.111TrpHis: 0.111 ± 0.071
0.668TrpIle: 0.668 ± 0.188
1.002TrpLys: 1.002 ± 0.205
1.447TrpLeu: 1.447 ± 0.353
0.779TrpMet: 0.779 ± 0.185
0.501TrpAsn: 0.501 ± 0.177
0.556TrpPro: 0.556 ± 0.2
1.28TrpGln: 1.28 ± 0.228
1.447TrpArg: 1.447 ± 0.302
0.723TrpSer: 0.723 ± 0.172
0.612TrpThr: 0.612 ± 0.207
1.057TrpVal: 1.057 ± 0.239
0.223TrpTrp: 0.223 ± 0.131
0.445TrpTyr: 0.445 ± 0.132
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.281TyrAla: 2.281 ± 0.365
0.389TyrCys: 0.389 ± 0.149
1.892TyrAsp: 1.892 ± 0.282
1.113TyrGlu: 1.113 ± 0.273
1.113TyrPhe: 1.113 ± 0.241
2.448TyrGly: 2.448 ± 0.377
0.612TyrHis: 0.612 ± 0.163
1.947TyrIle: 1.947 ± 0.326
1.224TyrLys: 1.224 ± 0.277
1.725TyrLeu: 1.725 ± 0.323
0.946TyrMet: 0.946 ± 0.254
1.447TyrAsn: 1.447 ± 0.303
1.335TyrPro: 1.335 ± 0.23
1.224TyrGln: 1.224 ± 0.249
2.281TyrArg: 2.281 ± 0.408
1.669TyrSer: 1.669 ± 0.231
1.781TyrThr: 1.781 ± 0.459
1.947TyrVal: 1.947 ± 0.326
0.668TyrTrp: 0.668 ± 0.184
1.224TyrTyr: 1.224 ± 0.21
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (17973 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski