Amino acid dipepetide frequency for Lachnoclostridium sp. An76

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.071AlaAla: 9.071 ± 0.148	1.119AlaCys: 1.119 ± 0.038	5.405AlaAsp: 5.405 ± 0.084	6.957AlaGlu: 6.957 ± 0.103	3.044AlaPhe: 3.044 ± 0.057	7.493AlaGly: 7.493 ± 0.114	1.161AlaHis: 1.161 ± 0.029	3.948AlaIle: 3.948 ± 0.078	4.223AlaLys: 4.223 ± 0.077	7.221AlaLeu: 7.221 ± 0.1	2.241AlaMet: 2.241 ± 0.051	2.187AlaAsn: 2.187 ± 0.054	2.241AlaPro: 2.241 ± 0.057	2.501AlaGln: 2.501 ± 0.057	3.371AlaArg: 3.371 ± 0.065	3.984AlaSer: 3.984 ± 0.069	2.612AlaThr: 2.612 ± 0.067	7.615AlaVal: 7.615 ± 0.122	0.66AlaTrp: 0.66 ± 0.033	2.867AlaTyr: 2.867 ± 0.061	0.0AlaXaa: 0.0 ± 0.0
Cys	1.124CysAla: 1.124 ± 0.038	0.27CysCys: 0.27 ± 0.019	0.757CysAsp: 0.757 ± 0.03	0.888CysGlu: 0.888 ± 0.031	0.617CysPhe: 0.617 ± 0.025	1.561CysGly: 1.561 ± 0.044	0.286CysHis: 0.286 ± 0.019	1.019CysIle: 1.019 ± 0.039	0.512CysLys: 0.512 ± 0.026	1.181CysLeu: 1.181 ± 0.038	0.471CysMet: 0.471 ± 0.024	0.408CysAsn: 0.408 ± 0.023	0.648CysPro: 0.648 ± 0.026	0.395CysGln: 0.395 ± 0.025	0.969CysArg: 0.969 ± 0.035	0.867CysSer: 0.867 ± 0.029	0.779CysThr: 0.779 ± 0.029	0.991CysVal: 0.991 ± 0.035	0.109CysTrp: 0.109 ± 0.01	0.508CysTyr: 0.508 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	4.707AspAla: 4.707 ± 0.081	0.763AspCys: 0.763 ± 0.031	3.105AspAsp: 3.105 ± 0.071	4.966AspGlu: 4.966 ± 0.078	2.512AspPhe: 2.512 ± 0.049	5.19AspGly: 5.19 ± 0.091	1.036AspHis: 1.036 ± 0.036	4.38AspIle: 4.38 ± 0.067	3.171AspLys: 3.171 ± 0.069	5.069AspLeu: 5.069 ± 0.083	1.868AspMet: 1.868 ± 0.045	2.031AspAsn: 2.031 ± 0.049	2.356AspPro: 2.356 ± 0.053	1.604AspGln: 1.604 ± 0.046	3.161AspArg: 3.161 ± 0.063	3.251AspSer: 3.251 ± 0.071	3.359AspThr: 3.359 ± 0.064	4.027AspVal: 4.027 ± 0.071	0.616AspTrp: 0.616 ± 0.027	2.772AspTyr: 2.772 ± 0.052	0.0AspXaa: 0.0 ± 0.0
Glu	6.127GluAla: 6.127 ± 0.101	0.889GluCys: 0.889 ± 0.033	4.848GluAsp: 4.848 ± 0.084	8.235GluGlu: 8.235 ± 0.126	2.544GluPhe: 2.544 ± 0.05	5.2GluGly: 5.2 ± 0.085	1.365GluHis: 1.365 ± 0.037	5.762GluIle: 5.762 ± 0.081	6.811GluLys: 6.811 ± 0.097	6.945GluLeu: 6.945 ± 0.098	2.604GluMet: 2.604 ± 0.048	4.046GluAsn: 4.046 ± 0.063	2.184GluPro: 2.184 ± 0.055	3.048GluGln: 3.048 ± 0.062	4.018GluArg: 4.018 ± 0.076	3.471GluSer: 3.471 ± 0.071	4.233GluThr: 4.233 ± 0.074	4.553GluVal: 4.553 ± 0.073	0.75GluTrp: 0.75 ± 0.03	3.414GluTyr: 3.414 ± 0.067	0.0GluXaa: 0.0 ± 0.0
Phe	2.96PheAla: 2.96 ± 0.059	0.734PheCys: 0.734 ± 0.029	2.513PheAsp: 2.513 ± 0.055	2.504PheGlu: 2.504 ± 0.055	1.73PhePhe: 1.73 ± 0.054	3.077PheGly: 3.077 ± 0.058	0.789PheHis: 0.789 ± 0.027	2.547PheIle: 2.547 ± 0.058	1.7PheLys: 1.7 ± 0.044	3.751PheLeu: 3.751 ± 0.077	1.107PheMet: 1.107 ± 0.039	1.283PheAsn: 1.283 ± 0.041	1.447PhePro: 1.447 ± 0.038	1.216PheGln: 1.216 ± 0.04	2.073PheArg: 2.073 ± 0.043	2.655PheSer: 2.655 ± 0.059	2.344PheThr: 2.344 ± 0.061	2.573PheVal: 2.573 ± 0.052	0.394PheTrp: 0.394 ± 0.023	1.615PheTyr: 1.615 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	5.767GlyAla: 5.767 ± 0.091	1.262GlyCys: 1.262 ± 0.039	4.111GlyAsp: 4.111 ± 0.084	5.746GlyGlu: 5.746 ± 0.083	3.055GlyPhe: 3.055 ± 0.06	5.535GlyGly: 5.535 ± 0.103	1.26GlyHis: 1.26 ± 0.037	6.014GlyIle: 6.014 ± 0.082	5.522GlyLys: 5.522 ± 0.079	6.003GlyLeu: 6.003 ± 0.086	2.596GlyMet: 2.596 ± 0.061	3.299GlyAsn: 3.299 ± 0.076	1.425GlyPro: 1.425 ± 0.045	2.482GlyGln: 2.482 ± 0.051	4.092GlyArg: 4.092 ± 0.073	4.693GlySer: 4.693 ± 0.079	4.703GlyThr: 4.703 ± 0.095	5.047GlyVal: 5.047 ± 0.073	0.791GlyTrp: 0.791 ± 0.042	3.487GlyTyr: 3.487 ± 0.072	0.0GlyXaa: 0.0 ± 0.0
His	1.112HisAla: 1.112 ± 0.035	0.279HisCys: 0.279 ± 0.018	0.873HisAsp: 0.873 ± 0.036	1.041HisGlu: 1.041 ± 0.035	0.753HisPhe: 0.753 ± 0.03	1.287HisGly: 1.287 ± 0.036	0.371HisHis: 0.371 ± 0.024	1.396HisIle: 1.396 ± 0.04	0.778HisLys: 0.778 ± 0.034	1.417HisLeu: 1.417 ± 0.041	0.544HisMet: 0.544 ± 0.023	0.653HisAsn: 0.653 ± 0.025	0.883HisPro: 0.883 ± 0.031	0.516HisGln: 0.516 ± 0.024	0.92HisArg: 0.92 ± 0.03	0.938HisSer: 0.938 ± 0.031	0.979HisThr: 0.979 ± 0.032	1.076HisVal: 1.076 ± 0.033	0.158HisTrp: 0.158 ± 0.015	0.771HisTyr: 0.771 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.142IleAla: 5.142 ± 0.082	1.12IleCys: 1.12 ± 0.035	3.965IleAsp: 3.965 ± 0.066	4.528IleGlu: 4.528 ± 0.079	2.566IlePhe: 2.566 ± 0.058	4.891IleGly: 4.891 ± 0.083	1.214IleHis: 1.214 ± 0.042	4.273IleIle: 4.273 ± 0.093	3.267IleLys: 3.267 ± 0.063	6.518IleLeu: 6.518 ± 0.106	1.728IleMet: 1.728 ± 0.043	2.392IleAsn: 2.392 ± 0.053	3.05IlePro: 3.05 ± 0.067	2.126IleGln: 2.126 ± 0.041	4.494IleArg: 4.494 ± 0.078	4.358IleSer: 4.358 ± 0.076	3.889IleThr: 3.889 ± 0.078	4.403IleVal: 4.403 ± 0.086	0.596IleTrp: 0.596 ± 0.028	2.678IleTyr: 2.678 ± 0.063	0.0IleXaa: 0.0 ± 0.0
Lys	4.806LysAla: 4.806 ± 0.077	0.654LysCys: 0.654 ± 0.027	3.62LysAsp: 3.62 ± 0.071	6.164LysGlu: 6.164 ± 0.088	1.691LysPhe: 1.691 ± 0.041	4.029LysGly: 4.029 ± 0.07	0.88LysHis: 0.88 ± 0.032	4.201LysIle: 4.201 ± 0.068	5.359LysLys: 5.359 ± 0.083	4.669LysLeu: 4.669 ± 0.072	2.039LysMet: 2.039 ± 0.054	2.911LysAsn: 2.911 ± 0.065	1.855LysPro: 1.855 ± 0.046	1.929LysGln: 1.929 ± 0.048	3.215LysArg: 3.215 ± 0.064	2.842LysSer: 2.842 ± 0.059	3.446LysThr: 3.446 ± 0.062	3.855LysVal: 3.855 ± 0.066	0.573LysTrp: 0.573 ± 0.026	2.741LysTyr: 2.741 ± 0.053	0.0LysXaa: 0.0 ± 0.0
Leu	7.116LeuAla: 7.116 ± 0.092	1.449LeuCys: 1.449 ± 0.041	5.342LeuAsp: 5.342 ± 0.078	6.564LeuGlu: 6.564 ± 0.089	3.533LeuPhe: 3.533 ± 0.071	6.11LeuGly: 6.11 ± 0.089	1.47LeuHis: 1.47 ± 0.04	5.659LeuIle: 5.659 ± 0.091	5.772LeuLys: 5.772 ± 0.08	8.052LeuLeu: 8.052 ± 0.124	2.496LeuMet: 2.496 ± 0.059	3.333LeuAsn: 3.333 ± 0.063	3.548LeuPro: 3.548 ± 0.067	2.427LeuGln: 2.427 ± 0.058	4.415LeuArg: 4.415 ± 0.083	5.807LeuSer: 5.807 ± 0.079	5.141LeuThr: 5.141 ± 0.07	5.144LeuVal: 5.144 ± 0.082	0.783LeuTrp: 0.783 ± 0.03	3.369LeuTyr: 3.369 ± 0.074	0.0LeuXaa: 0.0 ± 0.0
Met	2.397MetAla: 2.397 ± 0.061	0.351MetCys: 0.351 ± 0.018	2.007MetAsp: 2.007 ± 0.047	2.678MetGlu: 2.678 ± 0.058	0.946MetPhe: 0.946 ± 0.033	2.081MetGly: 2.081 ± 0.056	0.479MetHis: 0.479 ± 0.025	1.898MetIle: 1.898 ± 0.053	2.485MetLys: 2.485 ± 0.055	2.719MetLeu: 2.719 ± 0.059	0.84MetMet: 0.84 ± 0.029	1.302MetAsn: 1.302 ± 0.042	1.152MetPro: 1.152 ± 0.032	1.014MetGln: 1.014 ± 0.035	1.409MetArg: 1.409 ± 0.04	1.708MetSer: 1.708 ± 0.042	1.777MetThr: 1.777 ± 0.047	1.665MetVal: 1.665 ± 0.049	0.25MetTrp: 0.25 ± 0.016	0.922MetTyr: 0.922 ± 0.034	0.0MetXaa: 0.0 ± 0.0
Asn	3.007AsnAla: 3.007 ± 0.052	0.545AsnCys: 0.545 ± 0.024	1.907AsnAsp: 1.907 ± 0.043	2.509AsnGlu: 2.509 ± 0.049	1.325AsnPhe: 1.325 ± 0.036	3.418AsnGly: 3.418 ± 0.074	0.653AsnHis: 0.653 ± 0.026	2.875AsnIle: 2.875 ± 0.058	1.891AsnLys: 1.891 ± 0.048	3.291AsnLeu: 3.291 ± 0.067	1.185AsnMet: 1.185 ± 0.036	1.479AsnAsn: 1.479 ± 0.05	1.749AsnPro: 1.749 ± 0.048	1.101AsnGln: 1.101 ± 0.037	2.1AsnArg: 2.1 ± 0.056	2.122AsnSer: 2.122 ± 0.055	2.207AsnThr: 2.207 ± 0.053	2.609AsnVal: 2.609 ± 0.056	0.376AsnTrp: 0.376 ± 0.022	1.58AsnTyr: 1.58 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	2.983ProAla: 2.983 ± 0.064	0.464ProCys: 0.464 ± 0.024	2.753ProAsp: 2.753 ± 0.056	3.702ProGlu: 3.702 ± 0.066	1.514ProPhe: 1.514 ± 0.043	2.988ProGly: 2.988 ± 0.059	0.565ProHis: 0.565 ± 0.028	1.694ProIle: 1.694 ± 0.044	1.643ProLys: 1.643 ± 0.038	2.786ProLeu: 2.786 ± 0.059	0.843ProMet: 0.843 ± 0.033	1.101ProAsn: 1.101 ± 0.036	0.785ProPro: 0.785 ± 0.033	1.059ProGln: 1.059 ± 0.037	1.181ProArg: 1.181 ± 0.035	1.842ProSer: 1.842 ± 0.051	1.431ProThr: 1.431 ± 0.038	3.108ProVal: 3.108 ± 0.051	0.347ProTrp: 0.347 ± 0.019	1.46ProTyr: 1.46 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	2.626GlnAla: 2.626 ± 0.056	0.333GlnCys: 0.333 ± 0.016	1.722GlnAsp: 1.722 ± 0.045	2.694GlnGlu: 2.694 ± 0.059	1.103GlnPhe: 1.103 ± 0.037	2.104GlnGly: 2.104 ± 0.05	0.427GlnHis: 0.427 ± 0.021	2.553GlnIle: 2.553 ± 0.052	2.342GlnLys: 2.342 ± 0.05	2.521GlnLeu: 2.521 ± 0.064	1.157GlnMet: 1.157 ± 0.038	1.311GlnAsn: 1.311 ± 0.039	0.935GlnPro: 0.935 ± 0.031	1.12GlnGln: 1.12 ± 0.035	1.534GlnArg: 1.534 ± 0.044	1.601GlnSer: 1.601 ± 0.044	1.748GlnThr: 1.748 ± 0.043	1.981GlnVal: 1.981 ± 0.053	0.329GlnTrp: 0.329 ± 0.021	1.419GlnTyr: 1.419 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	3.51ArgAla: 3.51 ± 0.07	0.665ArgCys: 0.665 ± 0.03	2.805ArgAsp: 2.805 ± 0.063	4.993ArgGlu: 4.993 ± 0.088	2.192ArgPhe: 2.192 ± 0.039	3.064ArgGly: 3.064 ± 0.06	0.92ArgHis: 0.92 ± 0.032	3.897ArgIle: 3.897 ± 0.069	3.861ArgLys: 3.861 ± 0.069	4.574ArgLeu: 4.574 ± 0.094	1.818ArgMet: 1.818 ± 0.043	1.951ArgAsn: 1.951 ± 0.049	1.537ArgPro: 1.537 ± 0.052	2.006ArgGln: 2.006 ± 0.053	3.319ArgArg: 3.319 ± 0.08	2.593ArgSer: 2.593 ± 0.054	2.751ArgThr: 2.751 ± 0.057	3.033ArgVal: 3.033 ± 0.053	0.488ArgTrp: 0.488 ± 0.022	2.212ArgTyr: 2.212 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	4.7SerAla: 4.7 ± 0.07	0.799SerCys: 0.799 ± 0.033	3.373SerAsp: 3.373 ± 0.073	4.03SerGlu: 4.03 ± 0.066	2.42SerPhe: 2.42 ± 0.054	5.646SerGly: 5.646 ± 0.094	0.974SerHis: 0.974 ± 0.031	3.453SerIle: 3.453 ± 0.056	2.546SerLys: 2.546 ± 0.06	4.92SerLeu: 4.92 ± 0.081	1.671SerMet: 1.671 ± 0.041	1.667SerAsn: 1.667 ± 0.045	1.94SerPro: 1.94 ± 0.047	1.642SerGln: 1.642 ± 0.04	3.249SerArg: 3.249 ± 0.069	3.362SerSer: 3.362 ± 0.069	2.543SerThr: 2.543 ± 0.053	4.451SerVal: 4.451 ± 0.074	0.539SerTrp: 0.539 ± 0.023	2.314SerTyr: 2.314 ± 0.053	0.0SerXaa: 0.0 ± 0.0
Thr	4.95ThrAla: 4.95 ± 0.085	0.609ThrCys: 0.609 ± 0.026	3.614ThrAsp: 3.614 ± 0.07	4.393ThrGlu: 4.393 ± 0.078	2.128ThrPhe: 2.128 ± 0.049	5.227ThrGly: 5.227 ± 0.08	0.813ThrHis: 0.813 ± 0.027	3.133ThrIle: 3.133 ± 0.064	2.689ThrLys: 2.689 ± 0.045	4.675ThrLeu: 4.675 ± 0.072	1.402ThrMet: 1.402 ± 0.039	1.698ThrAsn: 1.698 ± 0.039	2.199ThrPro: 2.199 ± 0.05	1.449ThrGln: 1.449 ± 0.037	2.159ThrArg: 2.159 ± 0.049	2.66ThrSer: 2.66 ± 0.054	2.54ThrThr: 2.54 ± 0.072	4.955ThrVal: 4.955 ± 0.09	0.526ThrTrp: 0.526 ± 0.025	2.199ThrTyr: 2.199 ± 0.061	0.0ThrXaa: 0.0 ± 0.0
Val	4.243ValAla: 4.243 ± 0.075	1.275ValCys: 1.275 ± 0.033	3.835ValAsp: 3.835 ± 0.075	4.552ValGlu: 4.552 ± 0.07	3.118ValPhe: 3.118 ± 0.063	4.083ValGly: 4.083 ± 0.074	1.062ValHis: 1.062 ± 0.031	5.173ValIle: 5.173 ± 0.086	4.204ValLys: 4.204 ± 0.071	6.681ValLeu: 6.681 ± 0.096	2.06ValMet: 2.06 ± 0.055	2.706ValAsn: 2.706 ± 0.055	2.62ValPro: 2.62 ± 0.056	2.097ValGln: 2.097 ± 0.047	3.799ValArg: 3.799 ± 0.079	4.659ValSer: 4.659 ± 0.08	4.457ValThr: 4.457 ± 0.072	4.442ValVal: 4.442 ± 0.08	0.683ValTrp: 0.683 ± 0.029	2.86ValTyr: 2.86 ± 0.053	0.0ValXaa: 0.0 ± 0.0
Trp	0.618TrpAla: 0.618 ± 0.026	0.155TrpCys: 0.155 ± 0.013	0.615TrpAsp: 0.615 ± 0.025	0.732TrpGlu: 0.732 ± 0.028	0.435TrpPhe: 0.435 ± 0.021	0.67TrpGly: 0.67 ± 0.028	0.188TrpHis: 0.188 ± 0.014	0.663TrpIle: 0.663 ± 0.027	0.687TrpLys: 0.687 ± 0.025	0.912TrpLeu: 0.912 ± 0.034	0.333TrpMet: 0.333 ± 0.02	0.492TrpAsn: 0.492 ± 0.02	0.184TrpPro: 0.184 ± 0.014	0.386TrpGln: 0.386 ± 0.021	0.372TrpArg: 0.372 ± 0.022	0.479TrpSer: 0.479 ± 0.027	0.498TrpThr: 0.498 ± 0.027	0.499TrpVal: 0.499 ± 0.023	0.105TrpTrp: 0.105 ± 0.011	0.43TrpTyr: 0.43 ± 0.03	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.955TyrAla: 2.955 ± 0.062	0.625TyrCys: 0.625 ± 0.026	2.774TyrAsp: 2.774 ± 0.064	3.172TyrGlu: 3.172 ± 0.053	1.787TyrPhe: 1.787 ± 0.041	3.235TyrGly: 3.235 ± 0.067	0.833TyrHis: 0.833 ± 0.03	2.664TyrIle: 2.664 ± 0.052	2.013TyrLys: 2.013 ± 0.047	3.624TyrLeu: 3.624 ± 0.065	1.075TyrMet: 1.075 ± 0.032	1.679TyrAsn: 1.679 ± 0.042	1.509TyrPro: 1.509 ± 0.045	1.446TyrGln: 1.446 ± 0.043	2.339TyrArg: 2.339 ± 0.053	2.348TyrSer: 2.348 ± 0.052	2.582TyrThr: 2.582 ± 0.058	2.581TyrVal: 2.581 ± 0.052	0.38TyrTrp: 0.38 ± 0.023	1.929TyrTyr: 1.929 ± 0.056	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2953 proteins (975717 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---