Maize white line mosaic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Aureusvirus

Average proteome isoelectric point is 8.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5YVA8|A5YVA8_9TOMB Silencing suppressor protein OS=Maize white line mosaic virus OX=445227 PE=4 SV=1
MM1 pKa = 7.87ASPEE5 pKa = 4.04GWVLHH10 pKa = 6.14PNRR13 pKa = 11.84CDD15 pKa = 3.16LCGYY19 pKa = 8.25ATYY22 pKa = 10.68LRR24 pKa = 11.84EE25 pKa = 3.5SWRR28 pKa = 11.84YY29 pKa = 9.64GDD31 pKa = 4.09CQTHH35 pKa = 4.67RR36 pKa = 11.84RR37 pKa = 11.84IRR39 pKa = 11.84HH40 pKa = 4.89EE41 pKa = 4.7PIPSAVRR48 pKa = 11.84DD49 pKa = 3.54QGVQPWPWDD58 pKa = 3.31GTGGVSIAVLPANRR72 pKa = 11.84GISYY76 pKa = 7.47TLRR79 pKa = 11.84GHH81 pKa = 6.6GVTITVSGRR90 pKa = 11.84TDD92 pKa = 2.84NVLNITRR99 pKa = 11.84VANDD103 pKa = 3.49VFSHH107 pKa = 6.22PVIQGSICVRR117 pKa = 11.84GCAPTSVGCGPEE129 pKa = 3.85FLNEE133 pKa = 3.96NQKK136 pKa = 10.41EE137 pKa = 4.37VV138 pKa = 3.22

Molecular weight: 15.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5YVA6|A5YVA6_9TOMB Capsid protein OS=Maize white line mosaic virus OX=445227 PE=3 SV=1
MM1 pKa = 7.45ALARR5 pKa = 11.84GVLSQRR11 pKa = 11.84VVTAAVDD18 pKa = 3.68VTFGSVDD25 pKa = 3.31YY26 pKa = 10.81SDD28 pKa = 4.16PRR30 pKa = 11.84IVAALCDD37 pKa = 3.76GGLKK41 pKa = 10.19GRR43 pKa = 11.84ATVRR47 pKa = 11.84RR48 pKa = 11.84QIVTALKK55 pKa = 8.63WLVMVLTWPVRR66 pKa = 11.84MPAMAIVWCLTWVALMVTRR85 pKa = 11.84TTRR88 pKa = 11.84KK89 pKa = 7.43ICCVVSRR96 pKa = 11.84LYY98 pKa = 10.9SEE100 pKa = 4.95SSALVRR106 pKa = 11.84AYY108 pKa = 9.01WRR110 pKa = 11.84VYY112 pKa = 10.08NKK114 pKa = 8.48RR115 pKa = 11.84TRR117 pKa = 11.84AVACTGLVGSLALYY131 pKa = 10.34GPAAVLVWVCLLVVFVFCTLPADD154 pKa = 3.12ARR156 pKa = 11.84YY157 pKa = 9.63YY158 pKa = 10.33IKK160 pKa = 10.49LAKK163 pKa = 9.8KK164 pKa = 10.05IQDD167 pKa = 2.77AWDD170 pKa = 3.54AVEE173 pKa = 4.94EE174 pKa = 4.71DD175 pKa = 5.16DD176 pKa = 6.09SITPAADD183 pKa = 3.53GGPLEE188 pKa = 4.37VRR190 pKa = 11.84SGRR193 pKa = 11.84NRR195 pKa = 11.84FACRR199 pKa = 11.84LAARR203 pKa = 11.84AISRR207 pKa = 11.84VGLLKK212 pKa = 9.19PTKK215 pKa = 10.48ANALVYY221 pKa = 10.32QKK223 pKa = 11.18VILDD227 pKa = 3.55EE228 pKa = 4.24MKK230 pKa = 10.57VLNVRR235 pKa = 11.84FGDD238 pKa = 3.83RR239 pKa = 11.84VRR241 pKa = 11.84VLPLAVVACLEE252 pKa = 4.28RR253 pKa = 11.84PDD255 pKa = 4.16AVDD258 pKa = 3.1RR259 pKa = 11.84VEE261 pKa = 4.36GVIDD265 pKa = 5.4ALTCLPGSLL274 pKa = 4.4

Molecular weight: 30.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1768

138

797

353.6

38.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.05 ± 1.012

2.828 ± 0.311

4.638 ± 0.344

4.412 ± 0.643

3.337 ± 0.475

8.145 ± 1.02

1.301 ± 0.505

3.733 ± 0.487

4.186 ± 0.716

8.824 ± 0.975

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.206 ± 0.304

2.432 ± 0.495

5.204 ± 0.688

2.602 ± 0.286

7.919 ± 0.609

5.882 ± 0.695

7.579 ± 1.107

10.577 ± 0.932

1.81 ± 0.293

3.281 ± 0.319
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski