Drosophila melanogaster (Fruit fly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha;

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23524 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3Q7U5|A0A6M3Q7U5_DROME Fish-lips isoform E OS=Drosophila melanogaster OX=7227 GN=Fili PE=4 SV=1
MM1 pKa = 7.6ILNTTSFLLILVCQAWGQSIKK22 pKa = 10.4IRR24 pKa = 11.84IPVWDD29 pKa = 4.06ASTIFSRR36 pKa = 11.84LTGIGINSGNTNNQNPSNQANNNQQNVEE64 pKa = 4.13YY65 pKa = 10.84NDD67 pKa = 3.64YY68 pKa = 11.38LNNYY72 pKa = 8.42YY73 pKa = 10.93NNYY76 pKa = 7.64WASAYY81 pKa = 10.58QNVANYY87 pKa = 10.31DD88 pKa = 3.44PYY90 pKa = 11.44GYY92 pKa = 9.95YY93 pKa = 10.13SPYY96 pKa = 10.39GPYY99 pKa = 10.5GSYY102 pKa = 11.28APGQYY107 pKa = 7.79QTITTPTVTQTTTPMVPTTTAVISTTEE134 pKa = 3.96TPICAVFPHH143 pKa = 6.89LPACQTSTSATSDD156 pKa = 3.35STTSSTPMTTSTTTTEE172 pKa = 4.64RR173 pKa = 11.84ITASTSSTTTTSTEE187 pKa = 3.76LSTTSTAIPTSTTSEE202 pKa = 4.27STSTAIPTSSTTDD215 pKa = 2.91STTTSTTSPSTEE227 pKa = 3.85STTTSTAIPTSTTSEE242 pKa = 4.27STSTAIPTSSTTDD255 pKa = 2.91STTTSTTSSSTEE267 pKa = 3.77STTTSTAIPTSTTSEE282 pKa = 4.27STSTAIPTSSTTDD295 pKa = 2.91STTTSTTSSSTEE307 pKa = 3.77STTTSTAIPTSTTSEE322 pKa = 4.27STSTAIPTSSTTDD335 pKa = 2.91STTTSTTSSSTEE347 pKa = 3.77STTTSTAIPTSTTSEE362 pKa = 4.27STSTAIPTSSTTDD375 pKa = 2.91STTTSTTSSSTEE387 pKa = 3.77STTTSTAIPTSTTSEE402 pKa = 4.28STSTALPTSSSTDD415 pKa = 2.99STTTSTTSSSTEE427 pKa = 3.77STTTSTAIPNSTTSEE442 pKa = 4.25STSTAIPTSSTTDD455 pKa = 2.91STTTSTTSSSTEE467 pKa = 3.77STTTSTAIPTSTTSEE482 pKa = 4.28STSTALPTSSSTDD495 pKa = 2.99STTTSTTSSSTEE507 pKa = 3.77STTTSTAIPNSTTSEE522 pKa = 4.25STSTAIPTSSTTDD535 pKa = 2.91STTTSTTSSSTEE547 pKa = 3.77STTTSTAIPTSTTSEE562 pKa = 4.27STSTAIPTSSTTDD575 pKa = 2.91STTTSTTSSPTEE587 pKa = 3.85STTTSTAIPTSTTSEE602 pKa = 4.27STSTAIPTSSTTDD615 pKa = 3.11STKK618 pKa = 10.66TSTTSSSTEE627 pKa = 3.7SSTTSTAIPTSTTSEE642 pKa = 4.28STSTALPTSSSTDD655 pKa = 2.99STTTSTTSSSTEE667 pKa = 3.77STTTSTAIPNSTTSEE682 pKa = 4.25STSTAIPTSSTTDD695 pKa = 2.91STTTSTTSSSTEE707 pKa = 3.77STTTSTAIPTSTTSEE722 pKa = 4.27STSTAIPTSSTTDD735 pKa = 2.91STTTSTTSSPTEE747 pKa = 3.85STTTSTAIPTSTTSEE762 pKa = 4.27STSTAIPTSSTTDD775 pKa = 2.91STTTSTTSSSTEE787 pKa = 3.7SSTTSTAIPTSSTSDD802 pKa = 3.26SSDD805 pKa = 3.06STTTSTASPSDD816 pKa = 3.37STTTSTEE823 pKa = 3.95SSTSTTTEE831 pKa = 3.8STSTSTAIPTSSTSDD846 pKa = 3.13STTTSTASPSDD857 pKa = 3.37STTTSTEE864 pKa = 3.95SSTSTTTEE872 pKa = 3.8STSTSTAIPTSSTSDD887 pKa = 3.13STTTSTASSSDD898 pKa = 3.28SATTSTAIPTSTTTGGISTSTAIPQTKK925 pKa = 8.55STDD928 pKa = 4.04SICNEE933 pKa = 3.88YY934 pKa = 10.56PMLPNCQSEE943 pKa = 4.42KK944 pKa = 10.84AVNLKK949 pKa = 10.45KK950 pKa = 10.17IEE952 pKa = 4.46SNMFEE957 pKa = 4.47TNPEE961 pKa = 3.76VSVFKK966 pKa = 10.7PEE968 pKa = 3.79QKK970 pKa = 9.64LTITSMPQSVTSLCHH985 pKa = 6.32FYY987 pKa = 10.59PRR989 pKa = 11.84LCLKK993 pKa = 10.49PEE995 pKa = 3.74EE996 pKa = 4.2AQFVRR1001 pKa = 11.84LADD1004 pKa = 4.08EE1005 pKa = 4.15YY1006 pKa = 11.48GKK1008 pKa = 9.67PLLLISSIEE1017 pKa = 3.9GRR1019 pKa = 11.84SVYY1022 pKa = 9.92AQKK1025 pKa = 11.04LPALWRR1031 pKa = 11.84AIRR1034 pKa = 11.84RR1035 pKa = 11.84THH1037 pKa = 6.44RR1038 pKa = 11.84NKK1040 pKa = 10.79

Molecular weight: 105.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9V399|CP303_DROME Probable cytochrome P450 303a1 OS=Drosophila melanogaster OX=7227 GN=Cyp303a1 PE=2 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 7.85RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.6MRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 11.11

Molecular weight: 3.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13821

9703

23524

16289205

11

22949

692.5

76.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.421 ± 0.022

1.918 ± 0.049

5.196 ± 0.015

6.582 ± 0.039

3.293 ± 0.016

6.21 ± 0.022

2.644 ± 0.01

4.797 ± 0.015

5.504 ± 0.029

8.717 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.237 ± 0.014

4.735 ± 0.014

5.764 ± 0.038

5.455 ± 0.024

5.461 ± 0.017

8.61 ± 0.032

5.872 ± 0.027

5.864 ± 0.019

0.917 ± 0.007

2.799 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski