Amino acid dipepetide frequency for Arthrospira platensis (strain NIES-39 / IAM M-135) (Spirulina platensis)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.064AlaAla: 6.064 ± 0.085	0.718AlaCys: 0.718 ± 0.02	3.772AlaAsp: 3.772 ± 0.065	4.875AlaGlu: 4.875 ± 0.073	2.63AlaPhe: 2.63 ± 0.049	4.925AlaGly: 4.925 ± 0.064	1.193AlaHis: 1.193 ± 0.03	7.579AlaIle: 7.579 ± 0.109	3.553AlaLys: 3.553 ± 0.053	7.401AlaLeu: 7.401 ± 0.076	1.575AlaMet: 1.575 ± 0.036	3.257AlaAsn: 3.257 ± 0.064	2.564AlaPro: 2.564 ± 0.05	3.493AlaGln: 3.493 ± 0.05	3.403AlaArg: 3.403 ± 0.054	4.37AlaSer: 4.37 ± 0.085	4.48AlaThr: 4.48 ± 0.064	4.811AlaVal: 4.811 ± 0.07	0.95AlaTrp: 0.95 ± 0.026	2.174AlaTyr: 2.174 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.547CysAla: 0.547 ± 0.018	0.231CysCys: 0.231 ± 0.017	0.657CysAsp: 0.657 ± 0.021	0.569CysGlu: 0.569 ± 0.018	0.405CysPhe: 0.405 ± 0.015	0.909CysGly: 0.909 ± 0.027	0.379CysHis: 0.379 ± 0.016	0.551CysIle: 0.551 ± 0.02	0.348CysLys: 0.348 ± 0.015	1.14CysLeu: 1.14 ± 0.028	0.156CysMet: 0.156 ± 0.009	0.408CysAsn: 0.408 ± 0.015	0.631CysPro: 0.631 ± 0.02	0.721CysGln: 0.721 ± 0.025	0.645CysArg: 0.645 ± 0.02	0.606CysSer: 0.606 ± 0.018	0.478CysThr: 0.478 ± 0.017	0.563CysVal: 0.563 ± 0.022	0.235CysTrp: 0.235 ± 0.014	0.462CysTyr: 0.462 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	3.33AspAla: 3.33 ± 0.06	0.55AspCys: 0.55 ± 0.019	2.911AspAsp: 2.911 ± 0.076	3.053AspGlu: 3.053 ± 0.063	2.43AspPhe: 2.43 ± 0.043	3.751AspGly: 3.751 ± 0.103	0.969AspHis: 0.969 ± 0.025	3.88AspIle: 3.88 ± 0.059	1.987AspLys: 1.987 ± 0.037	5.912AspLeu: 5.912 ± 0.074	0.915AspMet: 0.915 ± 0.024	2.369AspAsn: 2.369 ± 0.037	2.589AspPro: 2.589 ± 0.052	1.998AspGln: 1.998 ± 0.038	4.097AspArg: 4.097 ± 0.054	3.366AspSer: 3.366 ± 0.201	2.624AspThr: 2.624 ± 0.053	2.766AspVal: 2.766 ± 0.071	0.99AspTrp: 0.99 ± 0.022	2.128AspTyr: 2.128 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	5.473GluAla: 5.473 ± 0.08	0.5GluCys: 0.5 ± 0.019	2.945GluAsp: 2.945 ± 0.053	3.917GluGlu: 3.917 ± 0.082	2.513GluPhe: 2.513 ± 0.043	3.388GluGly: 3.388 ± 0.054	0.965GluHis: 0.965 ± 0.025	4.884GluIle: 4.884 ± 0.063	3.169GluLys: 3.169 ± 0.05	6.762GluLeu: 6.762 ± 0.072	1.458GluMet: 1.458 ± 0.03	2.917GluAsn: 2.917 ± 0.046	2.862GluPro: 2.862 ± 0.096	3.074GluGln: 3.074 ± 0.052	3.205GluArg: 3.205 ± 0.064	3.809GluSer: 3.809 ± 0.075	4.108GluThr: 4.108 ± 0.142	4.157GluVal: 4.157 ± 0.067	0.862GluTrp: 0.862 ± 0.026	1.913GluTyr: 1.913 ± 0.038	0.0GluXaa: 0.0 ± 0.0
Phe	2.457PheAla: 2.457 ± 0.036	0.524PheCys: 0.524 ± 0.019	2.309PheAsp: 2.309 ± 0.04	2.25PheGlu: 2.25 ± 0.04	1.487PhePhe: 1.487 ± 0.037	2.755PheGly: 2.755 ± 0.055	0.776PheHis: 0.776 ± 0.023	2.364PheIle: 2.364 ± 0.047	1.654PheLys: 1.654 ± 0.032	3.868PheLeu: 3.868 ± 0.053	0.745PheMet: 0.745 ± 0.022	1.951PheAsn: 1.951 ± 0.038	1.938PhePro: 1.938 ± 0.039	1.788PheGln: 1.788 ± 0.038	2.04PheArg: 2.04 ± 0.037	2.726PheSer: 2.726 ± 0.044	2.164PheThr: 2.164 ± 0.039	2.203PheVal: 2.203 ± 0.042	0.748PheTrp: 0.748 ± 0.021	1.328PheTyr: 1.328 ± 0.024	0.0PheXaa: 0.0 ± 0.0
Gly	4.168GlyAla: 4.168 ± 0.071	0.885GlyCys: 0.885 ± 0.027	4.099GlyAsp: 4.099 ± 0.093	4.43GlyGlu: 4.43 ± 0.076	3.209GlyPhe: 3.209 ± 0.052	5.296GlyGly: 5.296 ± 0.102	1.291GlyHis: 1.291 ± 0.036	4.726GlyIle: 4.726 ± 0.064	3.829GlyLys: 3.829 ± 0.056	6.746GlyLeu: 6.746 ± 0.091	1.629GlyMet: 1.629 ± 0.038	3.31GlyAsn: 3.31 ± 0.074	1.422GlyPro: 1.422 ± 0.039	3.004GlyGln: 3.004 ± 0.048	3.618GlyArg: 3.618 ± 0.052	4.336GlySer: 4.336 ± 0.068	3.741GlyThr: 3.741 ± 0.063	4.897GlyVal: 4.897 ± 0.062	1.294GlyTrp: 1.294 ± 0.032	2.426GlyTyr: 2.426 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	0.852HisAla: 0.852 ± 0.024	0.359HisCys: 0.359 ± 0.016	0.9HisAsp: 0.9 ± 0.051	0.89HisGlu: 0.89 ± 0.026	0.726HisPhe: 0.726 ± 0.023	1.203HisGly: 1.203 ± 0.034	0.656HisHis: 0.656 ± 0.024	1.287HisIle: 1.287 ± 0.029	0.957HisLys: 0.957 ± 0.024	2.275HisLeu: 2.275 ± 0.044	0.218HisMet: 0.218 ± 0.012	0.928HisAsn: 0.928 ± 0.023	1.443HisPro: 1.443 ± 0.034	1.461HisGln: 1.461 ± 0.03	1.224HisArg: 1.224 ± 0.032	1.253HisSer: 1.253 ± 0.033	1.043HisThr: 1.043 ± 0.029	0.713HisVal: 0.713 ± 0.021	0.368HisTrp: 0.368 ± 0.017	0.681HisTyr: 0.681 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	7.045IleAla: 7.045 ± 0.093	0.773IleCys: 0.773 ± 0.022	4.008IleAsp: 4.008 ± 0.051	4.297IleGlu: 4.297 ± 0.059	2.525IlePhe: 2.525 ± 0.044	4.361IleGly: 4.361 ± 0.063	1.258IleHis: 1.258 ± 0.027	4.304IleIle: 4.304 ± 0.063	3.181IleLys: 3.181 ± 0.053	6.589IleLeu: 6.589 ± 0.075	1.071IleMet: 1.071 ± 0.025	3.563IleAsn: 3.563 ± 0.058	3.771IlePro: 3.771 ± 0.049	2.916IleGln: 2.916 ± 0.136	3.456IleArg: 3.456 ± 0.051	4.854IleSer: 4.854 ± 0.056	3.913IleThr: 3.913 ± 0.059	3.962IleVal: 3.962 ± 0.055	0.95IleTrp: 0.95 ± 0.025	2.209IleTyr: 2.209 ± 0.043	0.0IleXaa: 0.0 ± 0.0
Lys	3.689LysAla: 3.689 ± 0.062	0.446LysCys: 0.446 ± 0.017	1.825LysAsp: 1.825 ± 0.042	2.095LysGlu: 2.095 ± 0.039	1.669LysPhe: 1.669 ± 0.038	2.639LysGly: 2.639 ± 0.048	0.768LysHis: 0.768 ± 0.02	3.227LysIle: 3.227 ± 0.049	2.323LysLys: 2.323 ± 0.06	4.965LysLeu: 4.965 ± 0.066	0.966LysMet: 0.966 ± 0.027	1.986LysAsn: 1.986 ± 0.033	2.788LysPro: 2.788 ± 0.045	2.495LysGln: 2.495 ± 0.048	2.352LysArg: 2.352 ± 0.047	3.135LysSer: 3.135 ± 0.045	3.21LysThr: 3.21 ± 0.048	2.655LysVal: 2.655 ± 0.048	0.514LysTrp: 0.514 ± 0.018	1.358LysTyr: 1.358 ± 0.03	0.0LysXaa: 0.0 ± 0.0
Leu	8.228LeuAla: 8.228 ± 0.078	0.977LeuCys: 0.977 ± 0.024	5.389LeuAsp: 5.389 ± 0.061	7.377LeuGlu: 7.377 ± 0.084	3.487LeuPhe: 3.487 ± 0.06	7.838LeuGly: 7.838 ± 0.074	1.794LeuHis: 1.794 ± 0.04	6.804LeuIle: 6.804 ± 0.077	5.302LeuLys: 5.302 ± 0.058	10.281LeuLeu: 10.281 ± 0.093	2.278LeuMet: 2.278 ± 0.04	4.73LeuAsn: 4.73 ± 0.065	5.377LeuPro: 5.377 ± 0.062	5.241LeuGln: 5.241 ± 0.077	5.456LeuArg: 5.456 ± 0.069	7.532LeuSer: 7.532 ± 0.119	6.215LeuThr: 6.215 ± 0.068	6.679LeuVal: 6.679 ± 0.079	1.664LeuTrp: 1.664 ± 0.039	2.738LeuTyr: 2.738 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	2.069MetAla: 2.069 ± 0.037	0.131MetCys: 0.131 ± 0.008	0.787MetAsp: 0.787 ± 0.023	1.135MetGlu: 1.135 ± 0.03	0.582MetPhe: 0.582 ± 0.018	1.559MetGly: 1.559 ± 0.034	0.208MetHis: 0.208 ± 0.012	1.333MetIle: 1.333 ± 0.031	0.945MetLys: 0.945 ± 0.024	1.908MetLeu: 1.908 ± 0.035	0.558MetMet: 0.558 ± 0.021	0.89MetAsn: 0.89 ± 0.027	0.864MetPro: 0.864 ± 0.023	0.761MetGln: 0.761 ± 0.023	1.058MetArg: 1.058 ± 0.022	1.459MetSer: 1.459 ± 0.044	1.331MetThr: 1.331 ± 0.034	1.604MetVal: 1.604 ± 0.036	0.195MetTrp: 0.195 ± 0.012	0.458MetTyr: 0.458 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.954AsnAla: 2.954 ± 0.096	0.595AsnCys: 0.595 ± 0.02	1.934AsnAsp: 1.934 ± 0.057	1.692AsnGlu: 1.692 ± 0.039	1.822AsnPhe: 1.822 ± 0.035	2.714AsnGly: 2.714 ± 0.042	1.23AsnHis: 1.23 ± 0.029	2.922AsnIle: 2.922 ± 0.051	1.512AsnLys: 1.512 ± 0.036	5.783AsnLeu: 5.783 ± 0.084	0.8AsnMet: 0.8 ± 0.021	2.121AsnAsn: 2.121 ± 0.097	3.261AsnPro: 3.261 ± 0.051	3.016AsnGln: 3.016 ± 0.042	2.702AsnArg: 2.702 ± 0.048	3.144AsnSer: 3.144 ± 0.053	2.192AsnThr: 2.192 ± 0.037	2.044AsnVal: 2.044 ± 0.037	0.953AsnTrp: 0.953 ± 0.024	1.748AsnTyr: 1.748 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	2.79ProAla: 2.79 ± 0.047	0.36ProCys: 0.36 ± 0.016	3.429ProAsp: 3.429 ± 0.051	4.64ProGlu: 4.64 ± 0.094	1.583ProPhe: 1.583 ± 0.038	3.368ProGly: 3.368 ± 0.056	1.032ProHis: 1.032 ± 0.03	3.244ProIle: 3.244 ± 0.058	2.257ProLys: 2.257 ± 0.04	4.467ProLeu: 4.467 ± 0.055	0.903ProMet: 0.903 ± 0.023	2.476ProAsn: 2.476 ± 0.043	3.033ProPro: 3.033 ± 0.059	2.883ProGln: 2.883 ± 0.048	2.175ProArg: 2.175 ± 0.041	3.32ProSer: 3.32 ± 0.088	3.245ProThr: 3.245 ± 0.068	3.287ProVal: 3.287 ± 0.1	0.674ProTrp: 0.674 ± 0.02	1.455ProTyr: 1.455 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	4.253GlnAla: 4.253 ± 0.065	0.445GlnCys: 0.445 ± 0.019	2.218GlnAsp: 2.218 ± 0.037	3.378GlnGlu: 3.378 ± 0.057	1.846GlnPhe: 1.846 ± 0.035	3.342GlnGly: 3.342 ± 0.051	0.831GlnHis: 0.831 ± 0.021	3.595GlnIle: 3.595 ± 0.05	2.67GlnLys: 2.67 ± 0.053	5.986GlnLeu: 5.986 ± 0.08	1.089GlnMet: 1.089 ± 0.027	2.236GlnAsn: 2.236 ± 0.042	2.733GlnPro: 2.733 ± 0.051	3.736GlnGln: 3.736 ± 0.074	3.031GlnArg: 3.031 ± 0.135	2.922GlnSer: 2.922 ± 0.059	2.973GlnThr: 2.973 ± 0.048	3.605GlnVal: 3.605 ± 0.06	0.785GlnTrp: 0.785 ± 0.025	1.311GlnTyr: 1.311 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	3.299ArgAla: 3.299 ± 0.061	0.655ArgCys: 0.655 ± 0.023	3.016ArgAsp: 3.016 ± 0.05	3.554ArgGlu: 3.554 ± 0.058	2.443ArgPhe: 2.443 ± 0.04	3.429ArgGly: 3.429 ± 0.055	1.376ArgHis: 1.376 ± 0.031	3.468ArgIle: 3.468 ± 0.043	2.083ArgLys: 2.083 ± 0.036	6.51ArgLeu: 6.51 ± 0.11	1.035ArgMet: 1.035 ± 0.024	2.144ArgAsn: 2.144 ± 0.039	2.325ArgPro: 2.325 ± 0.038	3.544ArgGln: 3.544 ± 0.05	3.504ArgArg: 3.504 ± 0.059	3.487ArgSer: 3.487 ± 0.049	2.588ArgThr: 2.588 ± 0.041	3.68ArgVal: 3.68 ± 0.058	0.88ArgTrp: 0.88 ± 0.023	1.918ArgTyr: 1.918 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	4.098SerAla: 4.098 ± 0.06	0.64SerCys: 0.64 ± 0.021	3.998SerAsp: 3.998 ± 0.176	4.199SerGlu: 4.199 ± 0.15	2.221SerPhe: 2.221 ± 0.043	4.756SerGly: 4.756 ± 0.087	1.543SerHis: 1.543 ± 0.034	3.817SerIle: 3.817 ± 0.083	2.407SerLys: 2.407 ± 0.037	7.149SerLeu: 7.149 ± 0.07	1.3SerMet: 1.3 ± 0.031	2.626SerAsn: 2.626 ± 0.046	4.276SerPro: 4.276 ± 0.129	4.229SerGln: 4.229 ± 0.092	3.711SerArg: 3.711 ± 0.05	4.325SerSer: 4.325 ± 0.061	3.188SerThr: 3.188 ± 0.069	3.734SerVal: 3.734 ± 0.054	1.042SerTrp: 1.042 ± 0.028	2.052SerTyr: 2.052 ± 0.056	0.0SerXaa: 0.0 ± 0.0
Thr	4.508ThrAla: 4.508 ± 0.069	0.504ThrCys: 0.504 ± 0.017	2.705ThrAsp: 2.705 ± 0.049	3.664ThrGlu: 3.664 ± 0.06	1.963ThrPhe: 1.963 ± 0.039	4.286ThrGly: 4.286 ± 0.065	1.125ThrHis: 1.125 ± 0.04	4.044ThrIle: 4.044 ± 0.072	2.011ThrLys: 2.011 ± 0.038	6.179ThrLeu: 6.179 ± 0.082	0.942ThrMet: 0.942 ± 0.022	2.237ThrAsn: 2.237 ± 0.044	3.942ThrPro: 3.942 ± 0.1	2.817ThrGln: 2.817 ± 0.046	2.715ThrArg: 2.715 ± 0.05	3.333ThrSer: 3.333 ± 0.136	3.22ThrThr: 3.22 ± 0.062	3.86ThrVal: 3.86 ± 0.067	0.818ThrTrp: 0.818 ± 0.023	1.648ThrTyr: 1.648 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	5.28ValAla: 5.28 ± 0.074	0.683ValCys: 0.683 ± 0.022	3.334ValAsp: 3.334 ± 0.101	4.087ValGlu: 4.087 ± 0.071	2.483ValPhe: 2.483 ± 0.04	4.487ValGly: 4.487 ± 0.076	0.894ValHis: 0.894 ± 0.027	4.344ValIle: 4.344 ± 0.058	3.098ValLys: 3.098 ± 0.048	5.81ValLeu: 5.81 ± 0.062	1.39ValMet: 1.39 ± 0.034	3.024ValAsn: 3.024 ± 0.084	2.588ValPro: 2.588 ± 0.045	2.391ValGln: 2.391 ± 0.038	3.401ValArg: 3.401 ± 0.051	4.255ValSer: 4.255 ± 0.062	3.549ValThr: 3.549 ± 0.05	4.377ValVal: 4.377 ± 0.067	0.895ValTrp: 0.895 ± 0.027	1.842ValTyr: 1.842 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	0.932TrpAla: 0.932 ± 0.028	0.268TrpCys: 0.268 ± 0.015	0.812TrpAsp: 0.812 ± 0.025	1.21TrpGlu: 1.21 ± 0.03	0.638TrpPhe: 0.638 ± 0.023	1.154TrpGly: 1.154 ± 0.032	0.311TrpHis: 0.311 ± 0.014	0.791TrpIle: 0.791 ± 0.022	0.661TrpLys: 0.661 ± 0.018	2.071TrpLeu: 2.071 ± 0.04	0.339TrpMet: 0.339 ± 0.015	0.569TrpAsn: 0.569 ± 0.019	0.334TrpPro: 0.334 ± 0.016	1.027TrpGln: 1.027 ± 0.024	0.933TrpArg: 0.933 ± 0.026	0.964TrpSer: 0.964 ± 0.025	0.797TrpThr: 0.797 ± 0.023	1.148TrpVal: 1.148 ± 0.035	0.266TrpTrp: 0.266 ± 0.016	0.423TrpTyr: 0.423 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.748TyrAla: 1.748 ± 0.035	0.398TyrCys: 0.398 ± 0.016	1.57TyrAsp: 1.57 ± 0.033	1.57TyrGlu: 1.57 ± 0.039	1.411TyrPhe: 1.411 ± 0.029	2.196TyrGly: 2.196 ± 0.042	0.835TyrHis: 0.835 ± 0.024	1.843TyrIle: 1.843 ± 0.038	1.158TyrLys: 1.158 ± 0.029	3.586TyrLeu: 3.586 ± 0.045	0.467TyrMet: 0.467 ± 0.017	1.395TyrAsn: 1.395 ± 0.029	1.881TyrPro: 1.881 ± 0.038	2.39TyrGln: 2.39 ± 0.052	2.215TyrArg: 2.215 ± 0.042	2.116TyrSer: 2.116 ± 0.042	1.459TyrThr: 1.459 ± 0.033	1.468TyrVal: 1.468 ± 0.032	0.563TyrTrp: 0.563 ± 0.019	1.228TyrTyr: 1.228 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6009 proteins (1745523 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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