Klebsiella phage JD001

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Jedunavirus; Klebsiella virus JD001

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0ARS9|L0ARS9_9CAUD Uncharacterized protein OS=Klebsiella phage JD001 OX=1236000 PE=4 SV=1
MM1 pKa = 7.92AEE3 pKa = 4.19LTSTGYY9 pKa = 9.8SVKK12 pKa = 10.61SQNDD16 pKa = 3.0WFDD19 pKa = 3.59EE20 pKa = 4.25EE21 pKa = 4.28KK22 pKa = 10.67QLYY25 pKa = 10.35LDD27 pKa = 5.34IDD29 pKa = 4.75SNWNLDD35 pKa = 3.15PSTPDD40 pKa = 3.22GLKK43 pKa = 9.64IAHH46 pKa = 6.95DD47 pKa = 3.98AEE49 pKa = 4.46IFSALDD55 pKa = 3.24EE56 pKa = 4.48VLQQAYY62 pKa = 10.17NSKK65 pKa = 10.83DD66 pKa = 3.36PNKK69 pKa = 10.71ASGYY73 pKa = 10.2DD74 pKa = 3.51LDD76 pKa = 4.94VICALTGTVRR86 pKa = 11.84SEE88 pKa = 4.03GTASTVTGFVLTGTPGTQVPAGTRR112 pKa = 11.84FEE114 pKa = 4.52SSVTGYY120 pKa = 10.93RR121 pKa = 11.84FTLDD125 pKa = 3.18QTWTLDD131 pKa = 3.37SSGTATVDD139 pKa = 3.23ITCTTVGEE147 pKa = 4.34IEE149 pKa = 4.58ADD151 pKa = 3.23ANTITTIVDD160 pKa = 3.73TVAGLVSVNNPTPATPGTSAEE181 pKa = 4.23SDD183 pKa = 3.05GSLRR187 pKa = 11.84LKK189 pKa = 10.49RR190 pKa = 11.84ATAVGLPGSNQVDD203 pKa = 3.73SMLGQLFNVDD213 pKa = 2.55GVRR216 pKa = 11.84RR217 pKa = 11.84VRR219 pKa = 11.84VYY221 pKa = 11.09EE222 pKa = 4.02NDD224 pKa = 3.25EE225 pKa = 4.03ATTDD229 pKa = 3.55SNGQPGHH236 pKa = 6.53SIAPIVDD243 pKa = 3.38GGTDD247 pKa = 3.87DD248 pKa = 3.93NVAMAIYY255 pKa = 9.96LKK257 pKa = 10.46KK258 pKa = 10.73NPGVTLYY265 pKa = 10.7QAGTPVTVTVTSPTYY280 pKa = 8.95PTMTKK285 pKa = 9.97DD286 pKa = 3.14IKK288 pKa = 10.47FSRR291 pKa = 11.84PVYY294 pKa = 9.2VDD296 pKa = 2.87MVVVIEE302 pKa = 4.51IKK304 pKa = 10.65DD305 pKa = 3.9DD306 pKa = 3.66GTLPSQATLEE316 pKa = 4.24PLIQDD321 pKa = 4.96AIMEE325 pKa = 4.3YY326 pKa = 10.66AAGGLIPTEE335 pKa = 4.23YY336 pKa = 10.73GFKK339 pKa = 10.12PDD341 pKa = 3.92GFDD344 pKa = 2.96IGEE347 pKa = 4.3TVPYY351 pKa = 10.09SSLYY355 pKa = 9.26TPINKK360 pKa = 9.51VIGSYY365 pKa = 10.9GNSYY369 pKa = 9.48VNSMTLNGGTANVTIDD385 pKa = 3.64FNEE388 pKa = 4.08LSRR391 pKa = 11.84WTTSNITVTIVV402 pKa = 2.6

Molecular weight: 42.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0AQN9|L0AQN9_9CAUD Uncharacterized protein OS=Klebsiella phage JD001 OX=1236000 PE=4 SV=1
MM1 pKa = 7.49GSLVEE6 pKa = 5.26DD7 pKa = 4.22ILRR10 pKa = 11.84HH11 pKa = 5.25VPTKK15 pKa = 8.54QWRR18 pKa = 11.84KK19 pKa = 8.12KK20 pKa = 9.44CDD22 pKa = 3.71RR23 pKa = 11.84IASRR27 pKa = 11.84EE28 pKa = 3.89LHH30 pKa = 6.12LARR33 pKa = 11.84VPGIHH38 pKa = 6.4HH39 pKa = 7.56RR40 pKa = 11.84YY41 pKa = 8.84GKK43 pKa = 9.13WEE45 pKa = 3.77AVVFYY50 pKa = 11.0RR51 pKa = 11.84NVTLTIGCFNTPHH64 pKa = 7.12RR65 pKa = 11.84ALLARR70 pKa = 11.84KK71 pKa = 8.75LWYY74 pKa = 9.31FWRR77 pKa = 11.84EE78 pKa = 3.56RR79 pKa = 11.84GFTPQEE85 pKa = 3.75IPKK88 pKa = 9.88GPKK91 pKa = 8.95RR92 pKa = 11.84EE93 pKa = 4.08PYY95 pKa = 10.14SRR97 pKa = 11.84SS98 pKa = 3.19

Molecular weight: 11.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

14611

43

898

214.9

23.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.685 ± 0.471

1.383 ± 0.154

6.379 ± 0.199

5.496 ± 0.287

3.532 ± 0.192

7.672 ± 0.453

1.848 ± 0.165

5.893 ± 0.198

5.489 ± 0.342

7.535 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.614 ± 0.14

4.88 ± 0.28

3.997 ± 0.249

3.887 ± 0.264

5.065 ± 0.212

6.146 ± 0.267

7.002 ± 0.359

7.262 ± 0.266

1.554 ± 0.106

3.682 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski