Frondihabitans sp. PhB188

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Frondihabitans; unclassified Frondihabitans

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3539 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N1QKP1|A0A3N1QKP1_9MICO Uncharacterized protein OS=Frondihabitans sp. PhB188 OX=2485200 GN=EDF46_0686 PE=4 SV=1
MM1 pKa = 7.14VLAACTSGGTGAGPTASSAAGVGQGGATSRR31 pKa = 11.84SSDD34 pKa = 3.48PATPSPSPTGGAGALTATPLSVACDD59 pKa = 3.64TLAPDD64 pKa = 4.02AAVTAAYY71 pKa = 10.49AGMAPVTEE79 pKa = 4.43PDD81 pKa = 3.47VPAKK85 pKa = 9.96TDD87 pKa = 3.31AAVIGAYY94 pKa = 9.73QGTVCQWKK102 pKa = 10.3SDD104 pKa = 3.86TAVMTLAVGRR114 pKa = 11.84FDD116 pKa = 5.85DD117 pKa = 6.03DD118 pKa = 3.82SLTRR122 pKa = 11.84LKK124 pKa = 11.0NSLIRR129 pKa = 11.84TSNAVPTYY137 pKa = 10.48DD138 pKa = 3.47GEE140 pKa = 5.1GYY142 pKa = 10.41FDD144 pKa = 4.69LVGTTGTAEE153 pKa = 4.37AFTGSYY159 pKa = 8.09WVVAVSDD166 pKa = 4.2DD167 pKa = 3.92PVFGEE172 pKa = 4.29PGGAEE177 pKa = 4.19PLVDD181 pKa = 3.77AAITALQAGGG191 pKa = 3.37

Molecular weight: 18.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N1QFV8|A0A3N1QFV8_9MICO DNA-binding MarR family transcriptional regulator OS=Frondihabitans sp. PhB188 OX=2485200 GN=EDF46_1762 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3539

0

3539

1122767

31

1947

317.3

33.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.235 ± 0.058

0.452 ± 0.009

6.2 ± 0.038

5.165 ± 0.042

3.225 ± 0.025

8.992 ± 0.035

1.935 ± 0.018

4.407 ± 0.038

2.309 ± 0.031

9.96 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.699 ± 0.016

2.001 ± 0.02

5.288 ± 0.033

2.764 ± 0.023

6.833 ± 0.044

6.359 ± 0.038

6.641 ± 0.053

9.13 ± 0.044

1.399 ± 0.014

2.007 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski