Apis mellifera associated microvirus 47

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UUC8|A0A3S8UUC8_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 47 OX=2494777 PE=4 SV=1
MM1 pKa = 7.76LDD3 pKa = 3.43DD4 pKa = 4.1QGRR7 pKa = 11.84EE8 pKa = 4.11IPDD11 pKa = 3.48DD12 pKa = 3.84TPIVISIRR20 pKa = 11.84GQRR23 pKa = 11.84VSQFDD28 pKa = 3.17QVRR31 pKa = 11.84AYY33 pKa = 9.54IRR35 pKa = 11.84NEE37 pKa = 3.77LSRR40 pKa = 11.84QAEE43 pKa = 4.38GMQMEE48 pKa = 4.66TFEE51 pKa = 4.96DD52 pKa = 3.98ANDD55 pKa = 3.67FDD57 pKa = 5.75VEE59 pKa = 4.52DD60 pKa = 4.81DD61 pKa = 3.97MFPVSPHH68 pKa = 6.74EE69 pKa = 4.03YY70 pKa = 10.63SKK72 pKa = 10.56DD73 pKa = 3.27TEE75 pKa = 4.23EE76 pKa = 4.86ADD78 pKa = 4.46RR79 pKa = 11.84EE80 pKa = 4.18ALQAEE85 pKa = 5.13LEE87 pKa = 4.18RR88 pKa = 11.84QRR90 pKa = 11.84RR91 pKa = 11.84AKK93 pKa = 10.1AASSGKK99 pKa = 9.22PPSGNPDD106 pKa = 2.82GGAGSEE112 pKa = 4.47GSSPSPSEE120 pKa = 4.28PEE122 pKa = 4.08VQQ124 pKa = 3.55

Molecular weight: 13.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UUF5|A0A3S8UUF5_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 47 OX=2494777 PE=4 SV=1
MM1 pKa = 7.31SKK3 pKa = 10.33KK4 pKa = 10.21ARR6 pKa = 11.84RR7 pKa = 11.84STRR10 pKa = 11.84STEE13 pKa = 3.96PARR16 pKa = 11.84PPYY19 pKa = 10.38SGARR23 pKa = 11.84PSPAGSRR30 pKa = 11.84DD31 pKa = 3.23WPDD34 pKa = 3.37LLFSRR39 pKa = 11.84STVYY43 pKa = 10.17PRR45 pKa = 11.84STSVAHH51 pKa = 6.05RR52 pKa = 11.84ADD54 pKa = 5.44RR55 pKa = 11.84IRR57 pKa = 11.84QTLVAAYY64 pKa = 7.91QRR66 pKa = 11.84RR67 pKa = 11.84VAEE70 pKa = 4.51SLRR73 pKa = 11.84KK74 pKa = 9.83APIRR78 pKa = 11.84APGAFSFGGWSLPPSMLSQKK98 pKa = 10.15LQAASVCARR107 pKa = 11.84RR108 pKa = 11.84SVRR111 pKa = 11.84RR112 pKa = 11.84EE113 pKa = 3.41VLFATRR119 pKa = 11.84STGPGSHH126 pKa = 5.92ARR128 pKa = 11.84KK129 pKa = 8.51RR130 pKa = 11.84HH131 pKa = 4.54YY132 pKa = 10.94SNRR135 pKa = 11.84SCRR138 pKa = 3.55

Molecular weight: 15.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1351

124

474

270.2

29.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.881 ± 1.046

0.962 ± 0.522

4.589 ± 0.6

5.403 ± 0.808

3.701 ± 0.371

8.142 ± 0.666

2.591 ± 0.454

3.183 ± 0.372

3.035 ± 0.484

7.402 ± 0.74

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.443 ± 0.479

2.147 ± 0.272

6.366 ± 0.999

4.441 ± 0.731

9.03 ± 1.02

9.03 ± 0.949

6.07 ± 0.897

5.848 ± 0.476

1.406 ± 0.317

3.331 ± 0.675
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski