Gordonibacter pamelaeae 7-10-1-b

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Eggerthellales; Eggerthellaceae; Gordonibacter; Gordonibacter pamelaeae

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2027 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D6EAT7|D6EAT7_9ACTN Uncharacterized protein OS=Gordonibacter pamelaeae 7-10-1-b OX=657308 GN=GPA_28330 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.37SYY4 pKa = 10.8IEE6 pKa = 4.32PLNAADD12 pKa = 5.21ALDD15 pKa = 3.7VLDD18 pKa = 5.74LYY20 pKa = 11.56GIDD23 pKa = 3.43TPGAFFSSEE32 pKa = 3.96HH33 pKa = 5.9EE34 pKa = 4.33GCVEE38 pKa = 4.03VEE40 pKa = 4.27LSGHH44 pKa = 5.04TALLEE49 pKa = 4.36TPEE52 pKa = 4.17SVAMLFGHH60 pKa = 7.42DD61 pKa = 4.21AATALCQHH69 pKa = 6.27SNFRR73 pKa = 11.84RR74 pKa = 11.84DD75 pKa = 3.01AAEE78 pKa = 3.8IMSRR82 pKa = 11.84ALDD85 pKa = 4.23LDD87 pKa = 3.96DD88 pKa = 5.35FLYY91 pKa = 10.93NVYY94 pKa = 10.48SGTAFIPDD102 pKa = 3.6KK103 pKa = 11.24ASDD106 pKa = 3.87GEE108 pKa = 4.27FFGFHH113 pKa = 6.02VAHH116 pKa = 6.93IPLSAILEE124 pKa = 4.3DD125 pKa = 6.15DD126 pKa = 4.37IDD128 pKa = 4.31PLSPDD133 pKa = 3.06ACDD136 pKa = 3.65YY137 pKa = 11.37GLDD140 pKa = 4.19SISEE144 pKa = 4.34TTEE147 pKa = 3.86DD148 pKa = 3.39AAVGYY153 pKa = 8.96FDD155 pKa = 4.84PAGAVDD161 pKa = 4.72DD162 pKa = 4.89EE163 pKa = 4.85KK164 pKa = 11.33FLTSADD170 pKa = 3.22WALCDD175 pKa = 3.99IEE177 pKa = 5.94SSVLTAFGAGCSPEE191 pKa = 4.0LAGWTLGNAEE201 pKa = 4.75SVAEE205 pKa = 4.37TCASLGVDD213 pKa = 3.42IATDD217 pKa = 4.24LAPDD221 pKa = 3.43NPARR225 pKa = 11.84VAAAAKK231 pKa = 9.82AATKK235 pKa = 9.31ATTHH239 pKa = 5.63GVAAAAGNRR248 pKa = 11.84LL249 pKa = 3.39

Molecular weight: 25.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D6E6U0|D6E6U0_9ACTN Uncharacterized protein OS=Gordonibacter pamelaeae 7-10-1-b OX=657308 GN=GPA_04100 PE=4 SV=1
MM1 pKa = 7.59EE2 pKa = 4.7IVRR5 pKa = 11.84FAVGALLLVASALLAVQLWNGRR27 pKa = 11.84WQFLVARR34 pKa = 11.84PEE36 pKa = 3.98RR37 pKa = 11.84TKK39 pKa = 10.9KK40 pKa = 9.71GTFFPAGTRR49 pKa = 11.84EE50 pKa = 4.19TGRR53 pKa = 11.84HH54 pKa = 5.39LSWVMVACFAAVATLMASEE73 pKa = 4.55MGRR76 pKa = 11.84LSGSALFAQAGFVVSGVALVAYY98 pKa = 9.37GAFVVWTVAFHH109 pKa = 6.54CKK111 pKa = 9.63AHH113 pKa = 5.46GRR115 pKa = 11.84PYY117 pKa = 10.34FAGGSSRR124 pKa = 11.84LVVTLAATCAVLTALSLLFAA144 pKa = 5.61

Molecular weight: 15.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2027

0

2027

591018

18

3536

291.6

31.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.659 ± 0.084

1.621 ± 0.026

6.143 ± 0.057

6.757 ± 0.05

3.952 ± 0.039

8.332 ± 0.051

1.777 ± 0.023

4.828 ± 0.048

3.99 ± 0.049

9.298 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.766 ± 0.034

2.801 ± 0.035

4.43 ± 0.033

2.796 ± 0.029

5.981 ± 0.069

5.482 ± 0.042

5.083 ± 0.047

8.031 ± 0.057

1.24 ± 0.023

3.034 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski