Variola virus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 188 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q85376|Q85376_VARV Homolog of vaccinia virus CDS O1L; putative OS=Variola virus OX=10255 GN=Q1L PE=4 SV=1
MM1 pKa = 7.08YY2 pKa = 10.91NSMLPMFMCNNIVDD16 pKa = 5.42DD17 pKa = 5.2IDD19 pKa = 6.64DD20 pKa = 4.41IDD22 pKa = 6.7DD23 pKa = 4.29IDD25 pKa = 6.8DD26 pKa = 4.29IDD28 pKa = 6.8DD29 pKa = 4.29IDD31 pKa = 6.8DD32 pKa = 4.65IDD34 pKa = 6.85DD35 pKa = 4.29IDD37 pKa = 5.65DD38 pKa = 3.92KK39 pKa = 11.81ASNNDD44 pKa = 3.16DD45 pKa = 3.58HH46 pKa = 8.33NYY48 pKa = 10.23VYY50 pKa = 10.27PLPEE54 pKa = 3.79NMVYY58 pKa = 10.57RR59 pKa = 11.84FNKK62 pKa = 7.94STNILDD68 pKa = 3.87YY69 pKa = 11.21LSTEE73 pKa = 4.1RR74 pKa = 11.84DD75 pKa = 3.57HH76 pKa = 10.24VMMAVQYY83 pKa = 10.31YY84 pKa = 8.63MSKK87 pKa = 10.26QRR89 pKa = 11.84LDD91 pKa = 3.35DD92 pKa = 4.85LYY94 pKa = 11.09RR95 pKa = 11.84QLPTKK100 pKa = 8.67TRR102 pKa = 11.84SYY104 pKa = 10.88IDD106 pKa = 4.43IINMYY111 pKa = 9.31CDD113 pKa = 3.99KK114 pKa = 11.33VNNDD118 pKa = 3.47YY119 pKa = 11.25NRR121 pKa = 11.84DD122 pKa = 3.51MNIMYY127 pKa = 10.73DD128 pKa = 3.5MASTEE133 pKa = 4.21SFTVYY138 pKa = 10.63DD139 pKa = 3.93INNEE143 pKa = 3.95VNTILMDD150 pKa = 3.83NKK152 pKa = 10.79GLGVRR157 pKa = 11.84LATISFITEE166 pKa = 3.99LGKK169 pKa = 10.52RR170 pKa = 11.84CMNPVEE176 pKa = 4.32TIKK179 pKa = 10.52MFTLLSHH186 pKa = 6.92TICDD190 pKa = 3.93DD191 pKa = 3.62CFIDD195 pKa = 4.37YY196 pKa = 8.72ITDD199 pKa = 3.57ISPPDD204 pKa = 3.36NTIPNISTRR213 pKa = 11.84EE214 pKa = 3.87YY215 pKa = 10.62LKK217 pKa = 11.04LIGITAIMFATYY229 pKa = 7.95KK230 pKa = 8.27TLKK233 pKa = 10.56YY234 pKa = 10.15MIGG237 pKa = 3.33

Molecular weight: 27.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0N525|Q0N525_VARV A17L OS=Variola virus OX=10255 GN=A17L PE=3 SV=1
MM1 pKa = 7.19KK2 pKa = 10.33AKK4 pKa = 10.41RR5 pKa = 11.84NLEE8 pKa = 3.78RR9 pKa = 11.84EE10 pKa = 4.14IARR13 pKa = 11.84KK14 pKa = 9.35NCEE17 pKa = 3.85GNPCEE22 pKa = 5.71RR23 pKa = 11.84EE24 pKa = 3.93LEE26 pKa = 4.24YY27 pKa = 10.97EE28 pKa = 3.9RR29 pKa = 11.84SNVKK33 pKa = 10.2RR34 pKa = 11.84LEE36 pKa = 4.1YY37 pKa = 10.58QLDD40 pKa = 3.7AEE42 pKa = 4.28KK43 pKa = 10.93EE44 pKa = 4.0KK45 pKa = 11.21VKK47 pKa = 10.42FYY49 pKa = 10.87KK50 pKa = 10.29RR51 pKa = 11.84EE52 pKa = 4.09LEE54 pKa = 3.73RR55 pKa = 11.84DD56 pKa = 3.35RR57 pKa = 11.84YY58 pKa = 10.44LSSRR62 pKa = 11.84YY63 pKa = 8.1LTSSSDD69 pKa = 3.31PHH71 pKa = 6.52KK72 pKa = 10.57KK73 pKa = 9.65HH74 pKa = 5.54YY75 pKa = 10.34QIIHH79 pKa = 5.91FLALKK84 pKa = 9.69MYY86 pKa = 10.55LRR88 pKa = 11.84RR89 pKa = 11.84QLRR92 pKa = 11.84LQVLL96 pKa = 4.01

Molecular weight: 11.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

185

3

188

53522

63

1897

284.7

32.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.759 ± 0.114

2.117 ± 0.096

6.539 ± 0.122

5.304 ± 0.146

4.669 ± 0.137

3.948 ± 0.114

2.096 ± 0.091

9.346 ± 0.142

7.489 ± 0.106

8.849 ± 0.158

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.082

7.057 ± 0.12

3.357 ± 0.106

2.279 ± 0.085

4.266 ± 0.102

7.799 ± 0.15

6.188 ± 0.138

6.166 ± 0.108

0.678 ± 0.043

5.325 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski